Pannexin channels increase propidium iodide permeability in frozen–thawed dog spermatozoa

2017 ◽  
Vol 29 (11) ◽  
pp. 2269 ◽  
Author(s):  
J. L. Torres ◽  
J. Palomino ◽  
R. D. Moreno ◽  
M. De los Reyes
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Microscopy ◽  
Analysis Of Variance ◽  
Propidium Iodide ◽  
Membrane Channels ◽  
Flow Cytometry Data ◽  
Viable Cells ◽  
Head Segment ◽  
Relationship Of ◽  
The Relationship

Pannexins (Panx) are proteins that form functional single membrane channels, but they have not yet been described in dogs. The aim of the present study was to detect Panx1, Panx2 and Panx3 in frozen–thawed dog spermatozoa using flow cytometry and immunofluorescence analyses, evaluating the relationship of these proteins with propidium iodide (PI) in frozen–thawed spermatozoa. Fresh and frozen–thawed dog spermatozoa from eight dogs were preincubated with 3 μM PI with or without 15 μM carbenoxolone (CBX) or 1 mM probenecid (PBD), two Panx channel inhibitors, and then incubated with rabbit anti-Panx1, anti-Panx2 and anti-Panx3 antibodies (1 : 200). Panx immunolocalisation was assessed by fluorescence microscopy. Flow cytometry data were evaluated by analysis of variance. All three Panx proteins were found in dog spermatozoa: Panx1 was mostly localised to the acrosomal and equatorial segment, Panx2 was found in the posterior region of the head and tail and Panx3 was localised to the equatorial and posterior head segment. The percentage of PI-positive cells determined by flow cytometry was reduced (P < 0.05) in the presence of Panx inhibitors. These results show that Panx proteins are present in dog spermatozoa and increase PI permeability in frozen–thawed dog sperm, suggesting that the percentage of PI-positive spermatozoa used as an indicator of non-viable cells may lead to overestimation of non-viable cells.

Strategy and structure of the hotel industry in Malaysia

2007 ◽  
Vol 4 (1) ◽  
pp. 97
Author(s):  
Salleh Mohd Radzi ◽  
Mohamed Amran ◽  
Abdul Razak Aziz ◽  
Azlan Supardi
Keyword(s):  
Structural Equation Modeling ◽  
Analysis Of Variance ◽  
Structural Equation ◽  
Hotel Industry ◽  
Strategic Orientation ◽  
Equation Modeling ◽  
Major Purpose ◽  
Strategy And Structure ◽  
Relationship Of ◽  
The Relationship

The major purpose ofthis study was to examine the relationship of strategy and structure. Porter s(/980) strategic typology was utilized to classify hotel firms by strategic orientation; and, an analysis of variance was performed to determine the differences in their performance. Structural Equation Modeling was used to confirm the factors underlying the strategy and structure constructs.

Attitudes of Parents and Guardians towards Education, School and Teachers, and Children's Academic Achievement

1996 ◽  
Vol 79 (3_suppl) ◽  
pp. 1387-1390 ◽  
Author(s):  
Varghese I. Cherian ◽  
Lily Cherian
Keyword(s):  
Academic Achievement ◽  
Analysis Of Variance ◽  
Positive Relationship ◽  
Education School ◽  
Relationship Of ◽  
The Relationship

To study the relationship of parents' attitudes towards teachers, school, and education with the academic achievement of their children, a questionnaire was given to the parents of 1021 Standard 7 pupils (369 boys and 652 girls) randomly chosen from the Standard 7/Year 9 population of 14,765 boys and 26,109 girls. Analysis of variance indicated a positive relationship between parents' attitudes and the academic achievement of their children regardless of whether the children's parents were deceased or alive.

The ventricular-brain ratio (VBR) in functional psychoses: the relationship of lateral ventricular and total intracranial area

1990 ◽  
Vol 20 (1) ◽  
pp. 55-62 ◽  
Author(s):  
I. Harvey ◽  
M. Williams ◽  
B. K. Toone ◽  
S. W. Lewis ◽  
S. W. Turner ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Structural Changes ◽  
Sexual Differences ◽  
Ct Scans ◽  
Specific Difference ◽  
Functional Psychosis ◽  
Functional Psychoses ◽  
Community Controls ◽  
Relationship Of ◽  
The Relationship

SYNOPSISCT scans were performed on 72 consecutive patients with a functional psychosis, and compared with 50 community controls. There was a specific difference in lateral ventricular area between these two groups but it accounted for only 11% of the variance in this measure. In contrast, racial and sexual differences in the ventricular-brain ratio (VBR) related to differences in intra-cranial area. It is argued that analysis of variance, using lateral ventricular area and intra-cranial area, is more informative than VBR in examining these structural changes in functional psychoses.

The Relationship of Area Conservation to Area Measurement as Affected by Sequence of Presentation of Piagetian Area Tasks to Boys and Girls in Grades One through Three

1975 ◽  
Vol 6 (4) ◽  
pp. 232-242
Author(s):  
Thalia Taloumis
Keyword(s):  
Middle Class ◽  
Multiple Regression ◽  
Analysis Of Variance ◽  
Area Measurement ◽  
Pearson Product Moment Correlation ◽  
Statistical Procedures ◽  
Significant Difference ◽  
Relationship Of ◽  
The Relationship ◽  
Regression Correlation

Three area conservation tasks and two area measurement tasks, all representative models of area tasks reported by Piaget, were administered in one sitting to each of 168 middle-class children, ages six years five months to nine years four months. Statistical procedures included an analysis of variance, a Pearson product-moment correlation, and multiple-regression correlation for subgroups. The findings indicated (1) that whichever group of area tasks (area conservation tasks or area measurement tasks) was presented second, that group of tasks resulted in higher scores; (2) that there was a significant correlation between area conservation scores and area measurement scores; (3) that boys and girls did not score significantly differently on area conservation tasks and area measurement tasks; and (4) that scores in area conservation tasks and area measurement tasks showed no significant difference in amount of increase from grade to grade.

Collaboration or Appropriation? Examining a 17th c. Panel by David Teniers the Younger and Jan Brueghel the Younger Using Confocal X-Ray Fluorescence Microscopy

2007 ◽  
Vol 1047 ◽  
Author(s):  
Jennifer L. Mass ◽  
Arthur R. Woll ◽  
Noelle Ocon ◽  
Christina Bisulca ◽  
Tomasz Wazny ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cross Sections ◽  
Historical Research ◽  
Middle Ground ◽  
X Ray ◽  
Visual Elements ◽  
Bottom Left ◽  
Relationship Of ◽  
The Relationship

AbstractThe 17th c. Flemish painting on panel, The Armorer's Shop, has long been attributed to David Teniers the Younger (1610-1690). The painting depicts an opulent pile of parade armor at the bottom left foreground, a seated armorer at the bottom right foreground, and a forge surrounded by workers in the middle ground. The Teniers attribution is derived from his signature at the bottom right as well as figural groups and other visual elements that are commonly associated with him and executed in his style. During dendrochronological examination of the painting, a portion of the oak plank comprising the overall structure was found to have been carved out so that a smaller plank (containing the parade armor) could be inserted into the resulting depression. This unusual construction, combined with the identification of several paintings by Jan Brueghel the Younger (1601-1678) depicting the same parade armor, raised questions about the attribution and chronology of construction of the painting. Art historical research suggests that the smaller plank with the armor was painted by Brueghel and that the remainder of the panel with the workers and forge was painted by his brother-in-law Teniers. While Brueghel writes of collaborating with Teniers in his journal, this appears to be the only identified collaboration of the two artists. Conventional microanalysis methods did not resolve the painting's construction chronology. However, confocal x-ray fluorescence microscopy (CXRF) revealed the composition and location of buried paint layers at the panel interfaces by combining depth scans at a number of adjacent lateral positions to produce virtual cross-sections over 20 mm in length. The relationship of the paint layers at the panel interfaces provided evidence for the armor panel having been painted separately and prior to the rest of the composition. This data, along with dendrochronological and IRR data, provided a chronology of construction for the painting that provided additional evidence for a Brueghel attribution. An overview of the CXRF technique will be provided along with a discussion of how CXRF data relates to data collected using SEM-EDS, FTIR, Raman, conventional XRF, x-radiography, IRR, and dendrochronology.

dna flow cytometry of canine mammary tumours: the relationship of dna ploidy and S-phase fraction to clinical and histological features

1995 ◽  
Vol 58 (3) ◽  
pp. 238-243 ◽  
Author(s):  
M.D Péreze Alenza ◽  
G.R Rutteman ◽  
N.J Kuipers-Dijkshoorn ◽  
L Peña ◽  
A Montoya ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Flow Cytometry ◽  
Histological Features ◽  
Mammary Tumours ◽  
Relationship Of ◽  
The Relationship

A new method for the analysis of three-dimensional data matrices in agricultural experimentation

1974 ◽  
Vol 25 (5) ◽  
pp. 803 ◽  
Author(s):  
WT Williams ◽  
LA Edye
Keyword(s):  
Analysis Of Variance ◽  
Euclidean Distance ◽  
Three Dimensional ◽  
Real Life ◽  
Marine Ecology ◽  
New Method ◽  
Data Sets ◽  
Classical Statistics ◽  
Relationship Of ◽  
The Relationship

The analysis of three-dimensional data sets has received considerable attention in ecology, but relatively little in agriculture, in which such data sets are equally common. A model has recently been proposed for the (sites x species x times) case in marine ecology; it can be regarded as based either on Euclidean distance or on the analysis of variance. The model is recapitulated in outline, its properties are somewhat extended, and its application to agronomic experiments is discussed. A brief account is given of the use of the model in the analysis of two real-life agronomic experiments. Finally, the relationship of such methods to those of classical statistics is briefly discussed.

The relationship of flow cytometry results with classical measures of bacterial counts in raw refrigerated milk

2010 ◽  
Vol 63 (2) ◽  
pp. 297-300 ◽  
Author(s):  
LAERTE DAGHER CASSOLI ◽  
GRAZIELA FRANCISCHETTI ◽  
PAULO FERNANDO MACHADO ◽  
GERSON BARRETO MOURÃO
Keyword(s):  
Flow Cytometry ◽  
Bacterial Counts ◽  
Relationship Of ◽  
The Relationship

Usage and Influence of E-resources on Academic Efficiency in Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India

2020 ◽  
Vol 13 (2) ◽  
pp. 95-104
Author(s):  
J. Verma ◽  
◽  
N. Ahmad ◽  
Keyword(s):  
Data Collection ◽  
Analysis Of Variance ◽  
Correlation Coefficients ◽  
Statistical Techniques ◽  
Likert Scales ◽  
Access Points ◽  
Different Types ◽  
Data Collection Instrument ◽  
Relationship Of ◽  
The Relationship

The study discussed in this article was aimed to investigate the usages of e-resources, the relationship of access points with devices for accessing e-resources and influence of the use of e-resources on academic efficiency of faculty and technical staff of India’s first Agricultural University—Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India. The study also showed usage levels of different types of e-resources amongst users if access points are correlated with devices employed and the effect of e-resources on academic efficiency of academics. A questionnaire was used as a data collection instrument. The use of e-resources was studied using a method to scaling responses, following Likert Scales. Data obtained were converted into scores using scale rating. Standard statistical techniques were used for the analysis of variance, correlation coefficients, and others. Originality/value. This study will help understand the extent to which different e-resources can be used, the relationship between access points and devices used, and the influence of e-resources on academic efficiency. The librarians engaged in agricultural universities can also utilize findings of the study for carrying out acquisition and management of e-resources.

Accumulation of copper and silver onto cell body and its effect on the inactivation of Pseudomonas aeruginosa

2006 ◽  
Vol 54 (3) ◽  
pp. 29-34 ◽  
Author(s):  
M.G. Hwang ◽  
H. Katayama ◽  
S. Ohgaki
Keyword(s):  
Flow Cytometry ◽  
Pseudomonas Aeruginosa ◽  
Drinking Water ◽  
High Tolerance ◽  
Gram Negative ◽  
Syto 9 ◽  
Relationship Of ◽  
The Relationship ◽  
Specific Amount

Pseudomonas aeruginosa, a gram-negative rod bacterium, is a causative agent of waterborne pneumonia and presents high tolerance against conventional disinfectants. The inorganic biocidal reagents, copper and silver, were applied to inactivate P. aeruginosa inoculated in a synthetic drinking water (SDW). Additionally, the relationship of the specific amount of accumulated copper and silver reagents (Cs) on P. aeruginosa with inactivation profile was elucidated in this study. Flow cytometry (FCM) following staining with SYTO 9 and PI was used for detection of bacterial viability and density. Individual copper and silver reagents, and their combination, exhibited excellent biocidal abilities even at the concentration of 0.05 mgCu/L and 0.005 mgAg/L. The critical amounts of accumulated disinfectant (Cs) were calculated at 2.82×10−7 μgCu/cellsi and 5.13×10−8 μgAg/cellsi at an incubation of 70 h. Consequently, the role of disinfectant on the inactivation of P. aeruginosa and the assessment of biocidal ability of copper, silver, and their combination were successfully explained by evaluating the terms Cs and Cc.

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