Rosmarinic acid reverses the effects of metronidazole-induced infertility in male albino rats

2017 ◽  
Vol 29 (10) ◽  
pp. 1910 ◽  
Author(s):  
Zina M. Al-Alami ◽  
Ziad A. Shraideh ◽  
Mutasem O. Taha
Keyword(s):  
Rosmarinic Acid ◽  
Sperm Count ◽  
Biological Activities ◽  
Albino Rats ◽  
Distilled Water ◽  
Negative Effects ◽  
Ultrastructural Studies ◽  
Cauda Epididymidis ◽  
Sexually Mature ◽  
Harmful Effects

Rosmarinic acid (RA) is a natural antioxidant that has many biological activities. In the present study we investigated the potential of RA to reverse the negative effects of the widely used antibiotic and antiprotozoal agent metronidazole (MTZ), which is known to induce reversible male infertility. Two doses of RA (5 and 15 mg kg–1) were studied in sexually mature rats with and without MTZ-induced infertility. Rats were intraperitoneally injected with 5 mg kg–1 RA or 15 mg kg–1 RA (in distilled water) and, 45 min later, they were intraperitoneally injected with 40 mg kg–1 MTZ (in distilled water). Cauda epididymidal sperm suspensions were used to assess sperm count, motility and morphology. Histological and ultrastructural studies were performed on the testes and cauda epididymidis. In rats in which infertility was not induced, neither dose of RA affected the parameters assessed. However, in sexually mature rats in which infertility was induced by 40 mg kg–1 MTZ, RA at both 5 and 15 mg kg–1 ameliorated the damaging effects of MTZ on final bodyweight (30 days later), sperm motility and morphology. Only 5 mg kg–1 RA, and not 15 mg kg–1 RA, improved the harmful effects of MTZ on the sperm count and testis ultrastructure. The findings of the present study have considerable clinical implications and suggest a possible use for RA to reverse the negative effects of MTZ on male fertility, the male reproductive system and spermatogenesis.

The Impact of L-Carnitine and Coenzyme Q10 as Protection Against Busulfan-Oxidative Stress in the Liver of Adult Rats

2020 ◽  
Vol 10 (5) ◽  
pp. 578-586
Author(s):  
Areeg M. Abdelrazek ◽  
Shimaa A. Haredy
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Protective Role ◽  
Albino Rats ◽  
Distilled Water ◽  
Negative Effects ◽  
Adult Rats ◽  
Stress Damage ◽  
The Impact ◽  
Liver Tissues

Background: Busulfan (Bu) is an anticancer drug with a variety of adverse effects for cancer patients. Oxidative stress has been considered as a common pathological mechanism and it has a key role in the initiation and progression of liver injury by Bu. Aim: The study aimed to evaluate the antioxidant impact of L-Carnitine and Coenzyme Q10 and their protective role against oxidative stress damage in liver tissues. Methods and Material: Thirty-six albino rats were divided equally into six groups. G1 (con), received I.P. injection of DMSO plus 1 ml of distilled water daily by oral gavages; G2 (Bu), received I.P. injection of Bu plus 1 ml of the distilled water daily; G3 (L-Car), received 1 ml of L-Car orally; G4 (Bu + L-Car) received I.P. injection of Bu plus 1 ml of L-Car, G5 (CoQ10) 1 ml of CoQ10 daily; and G6 (Bu + CoQ10) received I.P. injection of Bu plus 1 ml of CoQ10 daily. Results: The recent data showed that Bu induced significant (P<0.05) elevation in serum ALT, AST, liver GSSG, NO, MDA and 8-OHDG, while showing significant (P<0.05) decrease in liver GSH and ATP. On the other hand, L-Carnitine and Coenzyme Q10 ameliorated the negative effects prompted by Bu. Immunohistochemical expression of caspase-3 in liver tissues reported pathological alterations in Bu group while also showed significant recovery in L-Car more than CoQ10. Conclusion: L-Car, as well as CoQ10, can enhance the hepatotoxic effects of Bu by promoting energy production in oxidative phosphorylation process and by scavenging the free radicals.

scholarly journals Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Godwin Adakole Ujah ◽  
Victor Udo Nna ◽  
Joseph Bagi Suleiman ◽  
Chinedum Eleazu ◽  
Chukwuemeka Nwokocha ◽  
...  
Keyword(s):  
Body Weight ◽  
Sperm Count ◽  
Reproductive Hormones ◽  
Nuclear Antigen ◽  
Albino Rats ◽  
Target Cells ◽  
Negative Effects ◽  
Related Proteins ◽  
Proliferating Cell ◽  
Cancerous Cells

AbstractDoxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. This study investigated the benefits of the antioxidant, tert-butylhydroquinone (tBHQ), on testicular toxicity following DOX therapy. Twenty-four adult male albino rats were assigned randomly into four groups (n = 6), namely: normal control (NC), tBHQ, DOX and tBHQ + DOX groups. tBHQ (50 mg/kg body weight in 1% DMSO) was administered orally for 14 consecutive days, while a single DOX dose (7 mg/kg body weight) was administered intraperitoneally on Day 8. DOX decreased sperm count, motility and viability, and decreased the levels of steroidogenesis-related proteins, and reproductive hormones. Furthermore, DOX decreased the expression of antioxidant cytoprotective genes, and decreased the protein level of proliferating cell nuclear antigen in the testis. Conversely, DOX increased the expression of pro-inflammatory and pro-apoptotic genes in the testis. These negative effects were ameliorated following the intervention with tBHQ. Our results suggest that tBHQ protects the testis and preserves both steroidogenesis and spermatogenesis in DOX-treated rats through the suppression of oxidative stress, inflammation and apoptosis.

Does rosmarinic acid treatment have protective role against sepsis-induced oxidative damage in Wistar Albino rats?

2016 ◽  
Vol 35 (8) ◽  
pp. 877-886 ◽  
Author(s):  
M Bacanlı ◽  
S Aydın ◽  
G Taner ◽  
HG Göktaş ◽  
T Şahin ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Damage ◽  
Rosmarinic Acid ◽  
Biological Activities ◽  
Albino Rats ◽  
Repair Capacity ◽  
Tnf Α ◽  
Liver And Kidney ◽  
Wistar Albino Rats ◽  
Α Level

Reactive oxygen species are believed to be involved in the development of sepsis. Plant-derived phenolic compounds are thought to be possible therapeutic agents against sepsis because of their antioxidant properties. Rosmarinic acid (RA) is a phenolic compound commonly found in various plants, which has many biological activities including antioxidant activity. The aim of this study was to investigate the effects of RA on sepsis-induced DNA damage in the lymphocytes and liver and kidney cells of Wistar albino rats by alkaline comet assay with and without formamidopyrimidine DNA glycosylase protein. The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels in the liver and kidney tissues and an inflammatory cytokine, tumor necrosis factor α (TNF-α) level in plasma were also evaluated. It is found that DNA damage in the lymphocytes, livers, and kidneys of the RA-treated rats was significantly lower than that in the sepsis-induced rats. RA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the livers and kidneys of the sepsis-induced rats. Plasma TNF-α level was found to be decreased in the RA-treated rats. It seems that RA might have a role in the attenuation of sepsis-induced oxidative damage not only by decreasing the DNA damage but also by increasing the antioxidant status and DNA repair capacity of the animals.

scholarly journals Spermatotoxic effects of selected traditional alcoholic beverages from North-Central Nigeria on adult male albino rats

2021 ◽  
Vol 2 (2) ◽  
pp. 014-022
Author(s):  
Olusayo Moritiwon ◽  
Timothy Olugbenga Ogundeko ◽  
David Oyebode ◽  
James Bitrus ◽  
Adikpe Emmanuel Edugbe ◽  
...  
Keyword(s):  
Sperm Count ◽  
Reproductive Age ◽  
Alcoholic Beverages ◽  
Albino Rats ◽  
Normal Male ◽  
Sperm Cells ◽  
Negative Effects ◽  
North Central ◽  
Caudal Portion

Challenges associated with habitual intake of alcohol including health, social, psychological and especially reproductive health needs urgent attention. This study aimed to determine the spermatotoxic effect of selected traditional alcoholic beverages in rats. A total of 30 normal male Spaque dawley strain albino rats weighing 180-220g, divided into 5 groups of 6 rats in each were administered with 10ml/kg p.o each of pito, goskolo and ogogoro, goskolo respectively and 0.5ml/kg normal saline for a period of 21 days. Sperm samples were harvested from the left caudal portion epididymis assayed for sperm motility, sperm morphology and sperm count after which histological examination was carried out on the testes. Results showed that active, sluggish and dead sperm cells were goskolo>pito>burukutu>control>ogogoro, ogogoro> burukutu> control>goskolo>pito and control>pito>ogogoro>burukutu>goskolo respectively. For morphology of sperm cells, it was goskolo>ogogoro>burukutu>control>pito (normal) and pito>control>burukutu> ogogoro> goskolo (abnormal). Also, that of sperm count was goskolo>ogogoro>pito>burukutu>control. Results further showed that ogogoro and goskolo caused significant negative effects on quantity and quality of sperm cells with alteration of histological parameters marked with altered secondary spermatogonia and spermatid. These effects were however mild with pito and burukutu. Traditional alcoholic beverages from North central Nigeria ‘pito’, 'burukutu', 'ogogoro', and goskolo' have negative on the quantity and quality of sperm cells with marked with infraction of spermatogonia of male albino rats. As a result of the spermatotoxic properties of these locally available and often ingested drinks by males predominantly in the reproductive age brackets will do well to avoid and or minimize its use as it leads to reprotoxicity.

scholarly journals The effectiveness of the protective role of beetroot juice for the male reproductive system of albino rats against the toxicity of cadmium chloride

2019 ◽  
Vol 24 (1) ◽  
pp. 43
Author(s):  
Qasim Azeez Razooqi AL-Janabi ◽  
Rashid Khamees Shaban ◽  
Noor Ibrahem Hasan AL-Kraie
Keyword(s):  
Cadmium Chloride ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Beetroot Juice ◽  
Negative Effects ◽  
Fourth Group ◽  
Abnormal Sperm

This study was designed to evaluate the protective role of beetroot juice against the toxicity of cadmium chloride (CdCl2) in male albino rats. The study included 20 male albino rats ages ranged between (2-3 months) and weights ranged between (220-260g), which were randomly distributed into 4 groups, the first group that has been promised to control group was given distilled water, the second group was given beetroot juice 10 ml/kg b.w., group third was given at a dose of cadmium chloride 5 mg/kg  b.w. for 30 days, which promised an infected control, and the fourth group were treated beetroot juice 10 ml/kg b. w. with cadmium chloride. The results showed that the treatment of animals cadmium chloride led group to a significant decrease (P ≤ 0.05) in the hormonal serum, to negative effects on of the natural shape of the sperm and to negative effects cleared on histological in testes tissues compared with the control group, In general has dosage rats treatment cadmium chloride juice of beetroot, to the positive improvement for most of the values ​​of previous indicators has led to increase significantly, the concentration of the LH, FSH and Testosterone hormone , to an improvement in the natural shape of the sperm, decreased significantly in the form abnormal sperm, led to an improvement cleared tissue in these totals became approach with what has been observed in the control group.   http://dx.doi.org/10.25130/tjps.24.2019.007     

The influence of media on upbringing and behavior of children

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 656-666
Author(s):  
Dr. Entisar Al-Obaidi
Keyword(s):  
New Technologies ◽  
The Internet ◽  
Negative Effects ◽  
Online Newspapers ◽  
Media Messages ◽  
Other Information ◽  
Family Home ◽  
Effects Of Media ◽  
And Behavior ◽  
Harmful Effects

Media refers to the channels of communication through which we distribute news, education, movies, music, advertising messages and other information. It includes physical and online newspapers and magazines, television, radio, telephone, the Internet, fax and billboards, are a dominant force in lives of children. Although television is remaining the predominant medium for children and adolescents, the new technologies are become more popular. We have to concern about the potential harmful effects of media "messages and images"; however, the positive and negative effects of media should be recognized. Parents have to establish the plan for all media in family home. Media that are influences on children should be recognized by "schools, policymakers, product advertisers, and entertainment producers".

scholarly journals Ocimum campechianum Mill. from Amazonian Ecuador: Chemical Composition and Biological Activities of Extracts and Their Main Constituents (Eugenol and Rosmarinic Acid)

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 84
Author(s):  
Massimo Tacchini ◽  
Monica Paulina Echeverria Guevara ◽  
Alessandro Grandini ◽  
Immacolata Maresca ◽  
Matteo Radice ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Syringae ◽  
Rosmarinic Acid ◽  
Plant Protection ◽  
Biological Activities ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Antioxidant Agents ◽  
Scavenger Activity

The essential oil (EO), the methanolic (MeOH), and the 70% ethanolic (70% EtOH) extracts obtained from the aerial parts of Ocimum campechianum Mill. (Ecuador) were chemically characterized through gas-chromatography coupled to mass spectrometry detector (GC-MS), high-performance liquid chromatography coupled to diode array-mass spectrometry detectors (HPLC-DAD-MS) and studied for their in vitro biological activity. The radical scavenger activity, performed by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, highlighted significant IC50 values for the EO, extracts and their main constituents (eugenol and rosmarinic acid). EO (and eugenol) showed noteworthy activity against Pseudomonas syringae pv. syringae and a moderate effect against clinical Candida strains, with possible synergism in association to fluconazole against the latter microorganisms. The extracts and pure molecules exhibited weak cytotoxic activity against the HaCat cell line and no mutagenicity against Salmonella typhimurium TA98 and TA100 strains, giving indication of safety. Instead, EO showed a weak activity against adenocarcinomic human alveolar basal epithelial cells (A549). The above-mentioned evidence leads us to suggest a potential use of the crude drug, extracts, and EO in cosmetic formulation and food supplements as antioxidant agents. In addition, EO may also have a possible application in plant protection and anti-Candida formulations.

scholarly journals The Chronic Effects of Narghile Use on Males’ Cardiovascular Response During Exercise: A Systematic Review

2021 ◽  
Vol 15 (2) ◽  
pp. 155798832199770
Author(s):  
Faten Chaieb ◽  
Helmi Ben Saad
Keyword(s):  
Systematic Review ◽  
Heart Rate ◽  
Cardiovascular Response ◽  
Recovery Period ◽  
Cardiopulmonary Exercise Test ◽  
Negative Effects ◽  
Chronic Effects ◽  
Blood Pressures ◽  
Response To Exercise ◽  
Harmful Effects

Narghile use has regained popularity throughout the world. Public opinion misjudges its chronic harmful effects on health, especially on the cardiovascular system. This systematic review aimed to evaluate the chronic effects of narghile use on cardiovascular response during exercise. It followed the preferred reporting items for systematic reviews guidelines. Original articles from PubMed and Scopus published until January 31, 2020, written in English, and tackling the chronic effects of narghile use on human cardiovascular response during exercise were considered. Five studies met the inclusion criteria. Only males were included in these studies. They were published between 2014 and 2017 by teams from Tunisia ( n = 4) and Jordan ( n = 1). One study applied the 6-min walk test, and four studies opted for the cardiopulmonary exercise test. Narghile use was associated with reduced submaximal (e.g., lower 6-min walk distance) and maximal aerobic capacities (e.g., lower maximal oxygen uptake) with abnormal cardiovascular status at rest (e.g., increase in heart rate and blood pressures), at the end of the exercise (e.g., lower heart rate, tendency to chronotropic insufficiency) and during the recovery period (e.g., lower recovery index). To conclude, chronic narghile use has negative effects on cardiovascular response to exercise with reduced submaximal and maximal exercise capacities.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

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