Recovery of fertility from adult ovarian tissue transplanted into week-old Japanese quail chicks

2015 ◽  
Vol 27 (2) ◽  
pp. 281 ◽  
Author(s):  
Jianan Liu ◽  
Kimberly M. Cheng ◽  
Frederick G. Silversides
Keyword(s):  
Japanese Quail ◽  
Sexual Maturity ◽  
Egg Production ◽  
In Vitro Maturation ◽  
Ovarian Tissue ◽  
Reproductive Potential ◽  
Special Importance ◽  
Old Japanese ◽  
Cryopreserved Ovarian Tissue

Fertility of cryopreserved ovarian tissue from immature chickens and Japanese quail has been recovered by transplantation. This is of special importance for non-mammalian vertebrates in which cryopreservation and in vitro maturation of oocytes are challenging because their oogenesis is characterised by vitellogenesis. This study tested whether fertility of adult quail ovarian tissue could be recovered by transplantation. Ovaries were isolated from mature Japanese quail hens, trimmed, cut into 3- to 4-mm2 pieces and transplanted into ovariectomised, week-old chicks. Recipients were administered an immunosuppressant for two weeks. Ten of 12 recipients survived until sexual maturity and seven laid eggs, but all stopped laying by 17 weeks of age. The age at first egg of recipients laying eggs (75.7 ± 4.2 days) was greater than that of untreated hens (51.8 ± 1.7 days) and egg production of recipients during the laying period (21.7 ± 5.7) was less than that of untreated hens (60.8 ± 3.5). Recipients were paired with males from the WB line for test mating. Only two hens laid eggs during the test period but both produced 100% donor-derived offspring. This research demonstrated that the reproductive potential of ovarian tissue from adult quail hens can be restored by transplantation.

In-vitro maturation of oocytes from ovarian tissue expands fertility preservation options

2011 ◽  
Vol 96 (3) ◽  
pp. S49
Author(s):  
L. Clark ◽  
W. Vitek ◽  
J. Witmyer ◽  
R. Hackett ◽  
S.A. Carson ◽  
...  
Keyword(s):  
Fertility Preservation ◽  
In Vitro Maturation ◽  
Ovarian Tissue

P–431 Human oocytes in-vitro maturation efficacy from infancy to adulthood – is there an optimal age?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
G Karavani ◽  
P Wasserzug-Pash ◽  
T Mordechai-Daniel ◽  
M Klutstein ◽  
T Imbar
Keyword(s):  
Fertility Preservation ◽  
In Vitro Maturation ◽  
Ovarian Tissue ◽  
Age Groups ◽  
Ovarian Tissue Cryopreservation ◽  
Success Rates ◽  
Human Oocytes ◽  
Tertiary Center ◽  
Significant Difference

Abstract Study question Does human oocytes in-vitro maturation (IVM) effectiveness change throughout childhood, adolescence and adulthood in girls and women undergoing fertility preservation via ovarian tissue cryopreservation (OTC) prior to chemo-radiotherapy exposure? Summary answer The optimal age for IVM is from menarche to 25 years, while pre-menarche girls and women older than 30 years have extremely low maturation rates. What is known already In vitro maturation of oocytes from antral follicles seen during tissue harvesting is a fertility preservation technique with potential advantages over OTC, as mature frozen and later thawed oocyte used for fertilization poses decreased risk of malignant cells re-seeding, as compared to ovarian tissue implantation. We previously demonstrated that IVM performed following OTC in fertility preservation patients, even in pre-menarche girls, yields a fair amount of oocytes available for IVM and freezing for future use. Study design, size, duration A retrospective cohort study, evaluating IVM outcomes in chemotherapy naïve patients referred for fertility preservation by OTC that had oocyte collected from the medium with attempted IVM between 2003 and 2020 in a university affiliated tertiary center. Participants/materials, setting, methods A total of 133 chemotherapy naïve patients aged 1–35 years with attempted IVM were included in the study. The primary outcome was IVM rate in the different age groups – pre-menarche (1–5 years and ≥6 years), post-menarche (menarche–17 years), young adults (18–24 years) and adults (25–29 and 30–35 years). Comparison between paired groups for significant difference in the IVM rate parameter was done using the Tukey’s Studentized Range (HSD) Test. Main results and the role of chance A gradual increase in mean IVM rate was demonstrated in the age groups over 1 to 25 years (4.6% (1–5 years), 23.8% (6 years to menarche) and 28.4% (menarche to 17 years), with a peak of 38.3% in the 18–24 years group, followed by a decrease in the 25–29 years group (19.3%), down to a very low IVM rate (8.9%) in the 30–35 years group. A significant difference in IVM rates was noted between the age extremes – the very young (1–5 years) and the oldest (30–35 years) groups, as compared with the 18–24-year group (p < 0.001). Number of oocytes matured, percent of patients with matured oocytes and overall maturation rate differed significantly (p < 0.001). Limitations, reasons for caution Data regarding ovarian reserve evaluation was not available for most of the patients, due to our pre-op OTC procedures protocol. None of our patients have used their frozen in-vitro matured oocytes, as such further implications of age on in-vitro matured oocytes quality and implantation potential has yet to be evaluated. Wider implications of the findings: Our finding of extremely low success rates in those very young (under 6 years) and older (≥30 years) patients suggest that IVM of oocyte retrieved during OTC prior to chemotherapy should not be attempted in these age group. Trial registration number N/A

First in vitro maturation (IVM) of oocytes retrieved from extracorporeal ovarian tissue aspiration leading to livebirth

2014 ◽  
Vol 102 (3) ◽  
pp. e126
Author(s):  
P.S. Uzelac ◽  
A.A. Delaney ◽  
G.L. Christensen ◽  
C.H.L. Bohler ◽  
S.T. Nakajima
Keyword(s):  
In Vitro Maturation ◽  
Ovarian Tissue

scholarly journals OVARIAN TISSUE OOCYTES IN VITRO MATURATION AS A METHOD OF FERTILITY PRESERVATION IN PATIENTS WITH GYNECOLOGICAL CANCER: A PILOT STUDY

2021 ◽  
Vol 116 (3) ◽  
pp. e2-e3
Author(s):  
Anastasia Kirillova ◽  
Ekaterina Bunyaeva ◽  
Nona Mishieva ◽  
Aydar Abubakirov ◽  
Johan E.J. Smitz
Keyword(s):  
Pilot Study ◽  
Fertility Preservation ◽  
In Vitro Maturation ◽  
Ovarian Tissue ◽  
Gynecological Cancer

scholarly journals New methods for the recovery of oocytes from bovine ovarian tissue in relation to in vitro maturation and fertilization.

1987 ◽  
Vol 33 (4) ◽  
pp. 188-192 ◽  
Author(s):  
Setsuo IWASAKI ◽  
Tomohiro KONO ◽  
Tatsuo NAKAHARA ◽  
Yasuo SHIOYA ◽  
Moriyuki FUKUSHIMA ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Ovarian Tissue ◽  
New Methods

scholarly journals Oncofertility case report: live birth 10 years after oocyte in vitro maturation and zygote cryopreservation

2020 ◽  
Vol 37 (12) ◽  
pp. 3089-3094
Author(s):  
P. Rodrigues ◽  
M. Marques ◽  
S. Pimentel ◽  
M. Rato ◽  
P. Carvalho ◽  
...  
Keyword(s):  
Live Birth ◽  
Ovarian Stimulation ◽  
In Vitro Maturation ◽  
Reproductive Potential ◽  
Growth Factor Receptor ◽  
Cumulus Cells ◽  
Healthy Baby ◽  
Immature Oocytes ◽  
Oncological Patient

Abstract Purpose This study aims to report a case of urgent fertility preservation in an oncological patient with collection of immature oocytes in the absence of ovarian stimulation that, through in vitro maturation (IVM), followed by ICSI and cryopreservation of zygotes resulted, 10 years later, in the live birth of a healthy baby. Methods In September 2008, our clinic performed IVM in a 32-year-old woman diagnosed with a ductal invasive carcinoma with positive estradiol receptors, negative progesterone receptors and positive human epidermal growth factor receptor 2. The retrieval of immature oocytes was performed in the absence of ovarian stimulation after a simple mastectomy and prior to any chemotherapy treatment. The compact cumulus-oocyte complexes (COCs) collected were placed in Lag medium for 2 h, followed by incubation in IVM medium, supplemented with heat inactivated patient serum, recombinant FSH, and recombinant LH. After 30 h in culture, cumulus cells were removed, the metaphase II oocytes were microinjected, and the zygotes obtained were cryopreserved. In 2017, the zygotes were thawed and cultured until day 3. One embryo was transferred and the other cryopreserved. Results Four compact COCs were collected and subjected to IVM. Two oocytes reached metaphase II and were microinjected. Two zygotes were obtained and were cryopreserved at the two pronuclear stage. Approximately 9 years later, the two zygotes were thawed and cultured until day 3. An embryo with 10 cells was transferred and implanted, resulting in the birth of a healthy baby. Conclusions In cases where urgency to start adjuvant therapy requires immediate oocyte collection, IVM may be the only option to obtain fully competent mature oocytes allowing for effective preservation of the reproductive potential.

Contrasting sizes at sexual maturity of southern rock lobsters (Jasus edwardsii) in the two Victorian fishing zones: implications for total egg production and management

1997 ◽  
Vol 48 (8) ◽  
pp. 1009 ◽  
Author(s):  
D. K. Hobday ◽  
T. J. Ryan
Keyword(s):  
Sexual Maturity ◽  
Egg Production ◽  
Carapace Length ◽  
Reproductive Potential ◽  
Size Class ◽  
Commercial Catch ◽  
Size Classes ◽  
Eastern Zone ◽  
Jasus Edwardsii ◽  
Western Zone

Fecundity (F) of Jasus edwardsii Hutton (Decapoda:Palinuridae) in the two Victorian fishing zones (Eastern and Western) was estimated from egg masses collected from 98 mature females (97–164 mm carapace length, CL) and was found to be related to carapace length (CL) by the equation F = 0·0316L3.359 (r2 = 0·8539; n = 571). Size at onset of sexual maturity (SOM), estimated from samples of the commercial catch (n = 3891) and analysed to determine the smallest size class in which 50% of females were carrying eggs or possessed ovigerous setae, was lower in the Western Zone (90 mm CL) than in the Eastern Zone (112 mm CL). Fecundity and SOM estimates and length frequencies in the commercial catch were used to estimate the relative reproductive potential (RRP) of each 5-mm-CL size class. The maximum RRP in the Western Zone was attributed to the size classes of 105 (38%) and 110 mm CL (25%), whereas the maximum RRP in the Eastern Zone was attributed to the size classes between 130 (40%) and 135 mm CL (16%). This study indicates that the current minimum legal size limits need to be reassessed and that separate management strategies for the two zones need to be considered.

Monitoring the ovaries after autotransplantation of cryopreserved ovarian tissue: endocrine studies, in vitro fertilization cycles, and live birth

2007 ◽  
Vol 87 (2) ◽  
pp. 418.e7-418.e15 ◽  
Author(s):  
Dror Meirow ◽  
Jacob Levron ◽  
Talia Eldar-Geva ◽  
Izhar Hardan ◽  
Eduard Fridman ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Live Birth ◽  
Ovarian Tissue ◽  
Vitro Fertilization ◽  
Cryopreserved Ovarian Tissue
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