A superovulation protocol for the spiny mouse (Acomys cahirinus)

2012 ◽  
Vol 24 (8) ◽  
pp. 1117 ◽  
Author(s):  
Rachael Pasco ◽  
David K. Gardner ◽  
David W. Walker ◽  
Hayley Dickinson
Keyword(s):  
Histological Analysis ◽  
Fold Increase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Spiny Mouse ◽  
Acomys Cahirinus ◽  
Oestrus Cycle ◽  
Ovulatory Follicles ◽  
Large Corpus

This study aimed to develop a superovulation protocol for the spiny mouse (Acomys cahirinus). The spiny mouse is a desert-adapted rodent species, with a long oestrus cycle (11 days) compared with rat and mouse, and gives birth to few (mean litter size is 3) precocial offspring after a relatively long gestation (39 days). We successfully optimised a superovulation protocol that elicited a 5-fold increase in the normal ovulation rate of this species. To induce superovulation in the spiny mouse 2 injections of equine chorionic gonadotrophin (eCG, 10 IU each), 9 h apart, were required, followed by 20 IU of human chorionic gonadotrophin (hCG). This protocol was successful in 100% of females trialed and at 33 h post-hCG an average of 14.7 ± 1.5, 1–2 cell embryos were recovered. Histological analysis of ovaries following superovulation revealed large corpus lutea and post-ovulatory follicles occupying a large part of the ovary. Ovulation commenced 6–12 h after the hCG injection and continued until 24–33 h post-hCG as indicated by both histological analysis of ovaries and the presence of oocytes/embryos in the oviduct. This superovulation protocol will facilitate the development of an in vitro culture system for spiny mouse embryos.

OESTROGEN BIOSYNTHESIS AND STEROID METABOLISM IN THE PORCINE OVARY

1969 ◽  
Vol 62 (3) ◽  
pp. 449-460 ◽  
Author(s):  
P. Preumont ◽  
I. D. Cooke ◽  
K. J. Ryan
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Steroid Metabolism ◽  
Corpora Lutea ◽  
17 Hydroxyprogesterone ◽  
Ovulatory Follicles ◽  
Cellular Compartments

ABSTRACT In vitro incubations of isolated porcine pre-ovulatory follicles, corpora lutea, and minced whole pre- and post-ovulatory ovaries were undertaken in an attempt to elucidate the pathways of oestrogen biosynthesis and steroid metabolism in the various cellular compartments of the porcine ovary. The conversion of radioactive acetate to cholesterol, the conversions of pregnenolone and progesterone to 16-hydroxyprogesterone, 17-hydroxyprogesterone, oestrone and oestradiol and the conversion of pregnenolone to 17-hydroxypregnenolone were observed following incubation with pre-ovulatory follicles in vitro. No significant effect of human chorionic gonadotrophin (HCG) added in vitro on steroid metabolism could be demonstrated. The conversions of dehydroepiandrosterone to oestradiol and 16-hydroxydehydroepiandrosterone to 16-hydroxyandrostenedione were obtained with corpora lutea in vitro. Pre-ovulatory whole ovarian mince incubations converted progesterone to 16-hydroxyprogesterone and androstenedione to oestrone. When 16-hydroxyandrostenedione, 16-hydroxyprogesterone and 16-hydroxydehydroepiandrosterone were incubated as substrates and/or formed in these various studies, no evidence for the formation of oestriol or other 16-oxygenated oestrogens could be demonstrated, although non-16 hydroxylated neutral steroids were aromatized in paired experiments.

Prolactin effects on basal and gonadotrophin-stimulated progesterone accumulation by PMSG-primed mouse ovaries in vitro

1985 ◽  
Vol 110 (4) ◽  
pp. 553-557 ◽  
Author(s):  
Julie A. Jonassen ◽  
Alan S. McNeilly
Keyword(s):  
Incubation Period ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Immature Female ◽  
Female Mice ◽  
Follicular Maturation ◽  
Progesterone Secretion ◽  
Ovulatory Follicles ◽  
Primed Mouse

Abstract. To examine the effects of prolactin (Prl) and human chorionic gonadotrophin (hCG) on progesterone production by murine ovarian explants, immature female mice were injected with 4 IU pregnant mare's serum gonadotrophin (PMSG) to induce follicular maturation. After 24 or 40 h mice were killed, ovaries removed, cut into fragments and maintained as explants for 24 h in the presence or absence of ovine or human Prl (25–2500 ng/ml). None of these doses of Prl affected basal progesterone accumulation into media over 24 h. To determine if Prl could modify the capacity of ovarian explants to respond to gonadotrophin, ovaries were incubated with 25 IU/ml hCG for 3 h after an initial 24 h incubation period with or without Prl. Prl had no effect on basal progesterone accumulation but significantly enhanced hCG-stimulated progesterone accumulation during the 3 h incubation period. We conclude that Prl does not inhibit but may enhance progesterone secretion by pre-ovulatory follicles in the mouse.

Insulin secretion in the spiny mouse (Acomys cahirinus). Dose and time kinetic studies with glucose in vivo and in vitro

Diabetes ◽  
1975 ◽  
Vol 24 (12) ◽  
pp. 1094-1100 ◽  
Author(s):  
A. Rabinovitch ◽  
A. Gutzeit ◽  
A. E. Renold ◽  
E. Cerasi
Keyword(s):  
Insulin Secretion ◽  
Kinetic Studies ◽  
Spiny Mouse ◽  
Acomys Cahirinus

STIMULATION BY HUMAN CHORIONIC GONADOTROPHIN OF OESTRADIOL PRODUCTION BY DISPERSED CELLS FROM HUMAN CORPUS LUTEUM: COMPARISON WITH PROGESTERONE PRODUCTION; UTILIZATION OF EXOGENOUS TESTOSTERONE

1981 ◽  
Vol 91 (2) ◽  
pp. 197-203 ◽  
Author(s):  
M. C. RICHARDSON ◽  
G. M. MASSON
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cell Suspensions ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Late Luteal Phase ◽  
Oestradiol Production ◽  
Dispersed Cells

Cell suspensions were prepared from tissue samples of human corpora lutea obtained during the mid- and late-luteal phase of the menstrual cycle. Both oestradiol and progesterone production by dispersed cells were stimulated by similar concentrations of human chorionic gonadotrophin (hCG). As the degree of stimulation of production by hCG was greater for progesterone than for oestradiol (five- to tenfold compared with two- to threefold higher than basal production), the ratio of progesterone to oestradiol produced varied according to the level of trophic stimulation. A comparison of cell suspensions prepared from mid- and late-luteal phase corpora lutea, exposed to the same concentration of hCG (10 i.u./ml) in vitro, did not reveal a shift to oestradiol production in the late-luteal phase. Provision of additional testosterone during incubation raised the level of oestradiol production by dispersed luteal cells. At an optimum concentration of testosterone (1 μmol/l), oestradiol synthesis was not raised further in the presence of hCG or N6, O2-dibutyryl cyclic AMP, suggesting a lack of induction or activation of the aromatase system by gonadotrophin in short-term cultures. Basal and stimulated levels of progesterone production were not significantly impaired in the presence of testosterone.

scholarly journals Association Between Serum Oestradiol Level on the Human Chorionic Gonadotrophin Administration Day and Neonatal Birthweight After in Vitro Fertilization-embryo Transfer: A Retrospective Study of 3659 Singleton Live Births

2020 ◽  
Author(s):  
Yu Liu ◽  
Jing Li ◽  
Yihong Guo
Keyword(s):  
Retrospective Study ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Vitro Fertilization ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Ivf Et ◽  
Group 1

Abstract BackgroundOestradiol, an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization-embryo transfer (IVF-ET) cycles. A supraphysiologic E2 level is inevitable during controlled ovarian hyper-stimulation (COH), and its effect on the outcome of IVF-ET is controversial. The aim of this retrospective study is to evaluate the association between elevated serum oestradiol (E2) levels on the day of human chorionic gonadotrophin (hCG) administration and neonatal birthweight after IVF-ET cycles.MethodsThe data of 3659 infertile patients with fresh IVF-ET cycles were analysed retrospectively between August 2009 and February 2017 in First Hospital of Zhengzhou University. Patients were categorized by serum E2 levels on the day of hCG administration into six groups: group 1 (serum E2 levels≤1000 pg/mL, n=230), group 2 (serum E2 levels between 1001 and 2000 pg/mL, n=524), group 3 (serum E2 levels between 2001 and 3000 pg/mL, n=783), group 4 (serum E2 levels between 3001 and 4000 pg/mL, n = 721), group 5 (serum E2 levels between 4001 and 5000 pg/mL, n=548 ), and group 6 (serum E2 levels > 5000 pg/mL, n=852). Univariate linear regression was used to evaluate the independent correlation between each factor and outcome index. Multiple logistic regression was used to adjust for confounding factors.ResultsThe LBW rates were as follows: 3.0% (group 1), 2.9% (group 2), 1.9% (group 3), 2.9% (group 4), and 2.0% (group 6) (P =0.629), respectively. There were no statistically significant differences in the incidences of neonatal LBW among the six groups. We did not detect an association between peak serum E2 level during ovarian stimulation and neonatal birthweight after IVF-ET.ConclusionThe results of this retrospective cohort study showed that serum E2 peak levels during ovarian stimulation were not associated with birth weight during IVF cycles. In addition, no association was found between higher E2 levels and increased LBW risk. Our observations suggest that the hyper-oestrogenic milieu during COS does not seem to have adverse effects on the birthweight of offspring after IVF.

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