Effect of cooled storage on quality and DNA integrity of Asian elephant (Elephas maximus) spermatozoa

2012 ◽  
Vol 24 (8) ◽  
pp. 1105 ◽  
Author(s):  
P. Imrat ◽  
S. Mahasawangkul ◽  
J. Gosálvez ◽  
P. Suthanmapinanth ◽  
P. Sombutputorn ◽  
...  
Keyword(s):  
Semen Quality ◽  
Storage Conditions ◽  
Quality Parameters ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Dna Stability ◽  
Subjective Impression ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Rate Of Increase

Artificial insemination (AI) is a potentially useful tool for breeding captive elephants because it facilitates efforts to minimise inbreeding. However, cooled storage of elephant semen markedly reduces fertility. This study compared the effects on semen-quality parameters, including sperm DNA fragmentation, of storing elephant semen at 4°C or 15°C in a commonly-used diluent (TEST) or a diluent developed to protect against sperm DNA damage (BullMax). Storing elephant semen for >24 h in either extender at either temperature resulted in decreases in sperm motility, viability, acrosome integrity and DNA integrity (P < 0.05); the decrease in motility was especially rapid. A subjective impression of circular sperm movement in TEST was confirmed by a higher curvilinear velocity and amplitude of lateral head displacement, but lower straight-line velocity and linearity than in BullMax. Initial percentages of spermatozoa with fragmented DNA (%SDF) did not differ between extenders or temperatures, but the rate of increase in %SDF during a 48-h incubation at 37°C was higher in TEST than in BullMax (P < 0.05). In conclusion, BullMax allows more linear movement and better preserves DNA stability of stored elephant spermatozoa than TEST. Sperm DNA stability during incubation at 37°C is a promising, discriminative parameter for selecting semen storage conditions of bulls for elephant AI.

scholarly journals Are semen quality parameters sufficient for biomonitoring spermatozoa DNA integrity and oxidatively damaged DNA

Biomonitoring ◽  
2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Hueiwang Anna Jeng ◽  
Ruei-Nian Li ◽  
Wen-Yi Lin
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Semen Quality ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Phase System ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna

Abstract:The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of 8-oxodGuo levels in sperm were related to the percentages of DNA fragmentation measured by both the TUNEL and SCSA (r = 0.22, p = 0.048; r = 0.12, p = 0.039). Sperm vitality, motility and morphology from all of the participants exhibited a weak correlation with the levels of 8-oxodGuo and the percentages of DNA fragmentation. Semen quality parameters may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Semen quality parameters may be insufficient to monitor sperm DNA fragmentation and oxidative damage. DNA damage in sperm is recommended to be included in routine measurements.

scholarly journals Urinary Concentrations of Parabens and Serum Hormone Levels, Semen Quality Parameters, and Sperm DNA Damage

2011 ◽  
Vol 119 (2) ◽  
pp. 252-257 ◽  
Author(s):  
John D. Meeker ◽  
Tiffany Yang ◽  
Xiaoyun Ye ◽  
Antonia M. Calafat ◽  
Russ Hauser
Keyword(s):  
Dna Damage ◽  
Semen Quality ◽  
Quality Parameters ◽  
Hormone Levels ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Serum Hormone

scholarly journals The aging male: Relationship between male age, sperm quality and sperm DNA damage in an unselected population of 3124 men attending the fertility centre for the first time

2019 ◽  
Vol 90 (4) ◽  
pp. 254-259 ◽  
Author(s):  
Alessandro Colasante ◽  
Maria Giulia Minasi ◽  
Filomena Scarselli ◽  
Valentina Casciani ◽  
Vincenzo Zazzaro ◽  
...  
Keyword(s):  
Dna Damage ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Quality ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Male Age ◽  
Sperm Dna

Objective: the aim of our study was to put forward insights to treat any possible correlation among sperm quality, sperm DNA damage and male age as they may have fertility implications for men who choose to delay fatherhood. Materials and methods: Our study is a non-interventional retrospective analysis of 3124 semen samples from patients that were investigated for the conventional semen parameters. Tunel test assay was set up for the evaluation of the sperm DNA fragmentation index (DFI). We applied the Kappa index to compare both the 1999 and the 2010 World Health Organization (WHO) reference criteria to evaluate the competence of such semen parameters categorization during the standard routine of our laboratory. Results: With regards to our findings, it is possible to underline a significant relationship between aging and semen volume (p = 0.001), motility (p = 0.009), semen viscosity (p < 0.003) and sperm DNA damage (p < 0.009). We found a trend when focusing on the semen concentration (p = 0.05). The analysis of sperm morphology did not show any influence with advancing age (p = 0.606). When comparing both the 1999 and the 2010 WHO scales we found no accordance in the appraisal of sperm morphology but a very good one in the evaluation of the other parameters. Conclusions: Conventional semen analysis represents the opportunity to draw up a proxy insight on the male fertility status even if semen quality can only indirectly assess the probability of pregnancy. Several studies have verified a decay in the male reproductive system, sperm quality and fertility with advancing age although the reported results are not yet conclusive. Our results substantially agree with those findings outlined in the literature. Moreover we find that the discrepancy between the two WHO reference scales would eventually lead to an improper diagnosis of infertility.

scholarly journals Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Essam-Elden M. Mohammed ◽  
Eman Mosad ◽  
Asmaa M. Zahran ◽  
Diaa A. Hameed ◽  
Emad A. Taha ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Acridine Orange ◽  
Pregnancy Outcome ◽  
Dna Fragmentation ◽  
Chromatin Condensation ◽  
Semen Parameters ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Sperm Dna

We evaluated the effect of varicocelectomy on semen parameters and levels of sperm DNA damage in infertile men. A total of 75 infertile men with varicocele and 40 fertile men (controls) were included in this study. Semen analysis and sperm DNA damage expressed as the DNA fragmentation index using acridine orange staining and chromatin condensation test by flow cytometry were assessed before and 6 months after varicocelectomy. The patients were also followed up for 1 year for pregnancy outcome. Semen parameters were significantly lower in varicocele patients compared to controls (P<0.05). Mean percentages of sperm DNA fragmentation and sperm DNA chromatin condensation in patients were significantly higher than those in controls (P<0.05). After varicocelectomy, sperm DNA fragmentation improved significantly, whereas sperm chromatin condensation was not significantly changed. In 15 out of 75 varicocele patients, clinical pregnancy was diagnosed; those with positive pregnancy outcome had significant improvement in sperm count, progressive sperm motility, and sperm DNA fragmentation, but there was no significant difference in sperm DNA condensation compared to negative pregnancy outcome patients. We concluded from this study that acridine orange stain is more reliable method than flow cytometry in the evaluation of sperm DNA integrity after varicocelectomy.

scholarly journals The effect of addition selected amino acids in extender semen on quality and DNA stability of frozen-thawed Sumba Ongole bull spermatozoa

2019 ◽  
Vol 24 (1) ◽  
Author(s):  
Syahruddin Said ◽  
Setiorini Setiorini ◽  
Amaitshaa Adella ◽  
Indah Sari ◽  
Nursafira Fathaniah ◽  
...  
Keyword(s):  
Amino Acids ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Dna Integrity ◽  
Freezing And Thawing ◽  
Dna Stability ◽  
Cattle Breeding ◽  
Egg Yolks

<p class="abstrak1"><span lang="IN">The objective of the current study was to asses the optimal concentration of glutamine, glycine and cysteine amino acids in tris-citric-acid-fructose egg yolks (TCFY) extender on quality of SO bull spermatozoa during freezing and thawing. </span><span lang="EN-US">In t</span><span lang="IN">his study the DNA stability of frozen-thawed Sperm</span><span lang="EN-US"> was </span><span lang="IN">also indentified. Three mature bulls maintained at PT. Karya Anugerah Rumpin, private cattle breeding company, West Java, Indonesia were used as semen donors. Semen was collected using artificial vagina and were evaluated </span><span lang="EN-US">prior</span><span lang="IN"> to freezing. Semen was diluted with TCFY supplemented with different concentrations of amino acids (5, 15 and 25 mM glycine and glutamine, and 3, 5 and 7 mM cysteine) then processed for colling and freezing. Semen quality parameters (subjective motility, viability and membrane and DNA integrity). </span><span lang="EN-US">D</span><span lang="IN">ata showed that in general the effect of addition of selected amino acids (glycine, glutamine and cysteine) </span><span lang="EN-US">in</span><span lang="IN">to TCFY extenders on motility, viability and membrane integrity of SO spermatozoa after cooling were significantly different (p&lt;0.05) higher than</span><span lang="EN-US"> that of</span><span lang="IN"> control. Addition of 15 mM glycine, 15 mM glutamine and 5 mM cysteine resulted in significant (p&lt;0.05) increase post-thawing sperm motility and sperm viability as compared to th</span><span lang="EN-US">at of</span><span lang="IN"> control. Furthermore, when spermatozoa were stained with acridine orange after fixation with acetic alcohol, the DNA integrity of post-thawing spermatozoa showed that all spermatozoa were remain intact. In conclusion </span><span lang="EN-US">,</span><span lang="IN">addition of 15 mM glycine, glutamine and 5 mM cysteine increase the cryoprotecting efficacy of bovine bull cryopreservation extender, and furthermore all DNA spermatozoa were remain intact. </span></p>

Sperm DNA fragmentation: etiology, pathogenesis, the influence on reproductive function

2020 ◽  
Vol 10 (4) ◽  
pp. 337-345
Author(s):  
Maxim N. Korshunov ◽  
Ekaterina S. Korshunova ◽  
Sergey P. Darenkov
Keyword(s):  
Dna Fragmentation ◽  
Reproductive Function ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Dna Breaks ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Recurrent Pregnancy Losses ◽  
Clinical Interest ◽  
The Relationship

The literature review of sperm DNA fragmentation is evaluated. Russian and foreign literary data over the past 10 years, including fundamental researching of the evaluation of the gametes genome integrity, are analyzed. The main etiological factors and the possible reasons of the DNA breaks formation on the different stages of spermatogenesis are described. The influence of the sperm oxidative stress reaction to the DNA integrity is analyzed. The relationship between DNA fragmentation of spermatozoa pregnancy and live birth rates in the assisted reproductive techniqueare noted. Risk of the recurrent pregnancy losses in male infertility cases with the sperm DNA damage is presented. Review confirms the significant prognostic value of sperm DNA fragmentation detection in infertility cases. Further studies in evaluation of pathogenesis of sperm DNA have a clinical interest to reproductive health physicians.

scholarly journals Human Semen Quality, Sperm DNA Damage, and the Level of Urinary Concentrations of 1N and TCPY, the Biomarkers of Nonpersistent Insecticides

2018 ◽  
Vol 13 (1) ◽  
pp. 155798831881659 ◽  
Author(s):  
Emila Dziewirska ◽  
Michał Radwan ◽  
Bartosz Wielgomas ◽  
Anna Klimowska ◽  
Paweł Radwan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Environmental Exposure ◽  
Semen Quality ◽  
Semen Analysis ◽  
Positive Association ◽  
Quality Parameters ◽  
Normal Morphology ◽  
Sperm Dna Damage ◽  
Fragmentation Index ◽  
Sperm Dna

The aim of the study was to evaluate the association between environmental exposure to nonpersistent insecticides and semen quality (concentration, motility, morphology, computer-aided semen analysis [CASA] parameters, and sperm DNA damage). Urine samples ( n = 315) collected from men who attended the infertility clinic with normal semen concentration of 15 to 300 mln/ml and age under 45 years were analyzed for two metabolites (1-naphthol [1N] and 3,5,6-trichloro-2-pyridinol [TCPY]) of nonpersistent insecticides. Participants provided semen, blood, and saliva samples; additionally, men filled a detailed questionnaire. The results identified that urinary TCPY concentration was significantly associated with a decrease in motility; also there was a positive association between TCPY and DNA fragmentation index (DFI). 1N concentration was negatively associated with a percentage of sperm with normal morphology and positively with one of the CASA parameters (curvilinear velocity [VCL]). The results suggest that environmental exposure to nonpersistent insecticides may have an impact on semen quality parameters and sperm DNA damage.

scholarly journals A Modified Flotation Density Gradient Centrifugation Technique Improves the Semen Quality of Stallions with a High DNA Fragmentation Index

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1973
Author(s):  
Muhammad Umair ◽  
Heiko Henning ◽  
Tom A. E. Stout ◽  
Anthony Claes
Keyword(s):  
Dna Fragmentation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Gradient Centrifugation ◽  
Quality Parameters ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Cushion Layer ◽  
Dna Fragmentation Index

Sperm DNA fragmentation compromises fertilization and early embryo development. Since spermatozoa lack the machinery to repair DNA damage, to improve the likelihood of establishing a healthy pregnancy, it is preferable to process ejaculates of stallions with a high sperm DNA fragmentation index (DFI) before artificial insemination or intracytoplasmic sperm injection. The aim of this study was to examine a modified flotation density gradient centrifugation (DGC) technique in which semen was diluted with a colloid solution (Opti-prepTM) to increase its density prior to layering between colloid layers of lower and higher density. The optimal Opti-prepTM solution (20–60%) for use as the bottom/cushion layer was first determined, followed by a comparison between a modified sedimentation DGC and the modified flotation DGC technique, using different Opti-prepTM solutions (20%, 25% and 30%) as the top layer. Finally, the most efficient DGC technique was selected to process ejaculates from Friesian stallions (n = 3) with high sperm DFI (>20%). The optimal Opti-prepTM solution for the cushion layer was 40%. The modified sedimentation technique resulted in two different sperm populations, whereas the modified flotation technique yielded three populations. Among the variants tested, the modified flotation DGC using 20% Opti-prepTM as the top layer yielded the best results; the average sperm recovery was 57%; the DFI decreased significantly (from 12% to 4%) and the other sperm quality parameters, including progressive and total motility, percentages of spermatozoa with normal morphology and viable spermatozoa with an intact acrosome, all increased (p < 0.05). In Friesian stallions with high sperm DFI, the modified flotation DGC markedly decreased the DFI (from 31% to 5%) and significantly improved the other semen quality parameters, although sperm recovery was low (approximately 20%). In conclusion, stallion sperm DFI and other sperm quality parameters can be markedly improved using a modified flotation DGC technique employing a 40% Opti-prepTM cushion and a 20% top layer.

scholarly journals Sperm DNA Integrity Assessment: A New Tool in Diagnosis and Treatment of Fertility

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Mona Bungum
Keyword(s):  
Semen Quality ◽  
Significant Proportion ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Integrity Assessment ◽  
Sperm Dna Integrity ◽  
Sperm Dna

Infertility affects 15% of all couples. Although male infertility factors with reduced semen quality are contributing to about half of all involuntary childlessness, the value of standard semen parameters in prediction of fertilityin vivoand choice of proper method for assisted reproduction is limited. In the search for better markers of male fertility, during the last 10 years, assessment of sperm DNA integrity has emerged as a strong new biomarker of semen quality that may have the potential to discriminate between infertile and fertile men. Sperm DNA Fragmentation Index (DFI) as assessed by the flow cytometric Sperm Chromatin Structure Assay (SCSA) can be used for evaluation of sperm chromatin integrity. The biological background for abnormal DFI is not completely known, but clinical data show that DFI above 30% is associated with very low chance for achieving pregnancy in natural way or by insemination, but notin vitro. Already when the DFI is above 20%, the chance of natural pregnancy may be reduced, despite other sperm parameters being normal. Thus this method may explain a significant proportion of cases of unexplained infertility and can be beneficial in counselling involuntary childless couples need ofin vitrofertilisation.

Effect of in vitro exposure to lead chloride on semen quality and sperm DNA fragmentation

Zygote ◽  
2014 ◽  
Vol 23 (3) ◽  
pp. 384-393 ◽  
Author(s):  
M. Gomes ◽  
A. Gonçalves ◽  
E. Rocha ◽  
R. Sá ◽  
A. Alves ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Room Temperature ◽  
Semen Quality ◽  
Lead Chloride ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Progressive Motility ◽  
Sperm Dna ◽  
In Vitro Exposure

SummaryExposure to lead may cause changes in the male reproductive system. We evaluated the effect of lead chloride (PbCl2) in vitro on semen quality from 31 individuals. Samples were incubated at room temperature for two exposure times (4 h and 8 h) and with two concentrations of PbCl2 (15 μg/ml or 30 μg/ml). Results showed that PbCl2 significantly inhibited rapid progressive motility and caused an increase in the percentage of tail anomalies in both times and concentrations assessed, as well as a decrease in vitality in the group exposed to 30 μg/ml PbCl2. A significant increase in immotile sperm was also observed between the group control and the groups submitted to lead. Total motility and DNA fragmentation also showed a significant decrease and increase, respectively, after 4 h of incubation in the group exposed to 30 μg/ml and in both groups after 8 h of incubation. In conclusion, PbCl2 affected sperm parameters and DNA integrity, which are essential for male fertility.

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