Effect of Uv Radiation on Endogenous Inhibitors of Growth in the Hypocotyl of Phaseolus vulgaris

1994 ◽  
Vol 21 (2) ◽  
pp. 235 ◽  
Author(s):  
H Kato-Noguchi ◽  
M Sumitomo
Keyword(s):  
Phaseolus Vulgaris ◽  
Uv Radiation ◽  
Time Course ◽  
Phaseolus Vulgaris L ◽  
Inhibition Of Growth ◽  
Endogenous Inhibitors ◽  
Bean Plants ◽  
The Difference ◽  
Acetone Extracts

In order to correlate the role of endogenous inhibitors of growth of bean plants with the inhibition of such growth by UV radiation, a search for specific growth inhibitors was undertaken using the neutral and acidic fractions of acetone extracts from the hypocotyls of UV-irradiated bean seedlings (Phaseolus vulgaris L. cv. Morocco). Tho neutral and two acidic inhibitors were isolated and they were named N-1 and N-2 (neutral inhibitors) and A-1 and A-2 (acidic inhibitors), respectively, on the basis of the order of their elution from a silica-gel column. Variations in the activity of the inhibitors in hypocotyls of two cultivars (cvv. Morocco and Kentucky 101) after the onset of UV radiation were determined by use of a bean bioassay, and results were compared with those of UV-induced inhibition of growth. The growth of hypocotyls of the two cultivars decreased rapidly. However, the more noticeable change was a marked decrease in the growth of the hypocotyls of cv. Morocco. On the other side, the difference in the activity of N-1 between the two cultivars was particularly great, and the changes with time in levels of N-1 in hypocotyls of each cultivar matched the time course of the UV-induced inhibition of growth of hypocotyls of the same cultivar. No such correlation was found with N-2, A-1 and A-2. These results suggest that the activity of N-1 may serve to distinguish the growth habit of the two cultivars to UV radiation.

Perception and first defense responses against Pseudomonas syringae pv. phaseolicola in Phaseolus vulgaris L.: identification of Wall-Associated Kinase receptors

2021 ◽  
Author(s):  
Alfonso Gonzalo De la Rubia ◽  
María Luz Centeno ◽  
Victor Moreno-González ◽  
María De Castro ◽  
Penélope García-Angulo
Keyword(s):  
Phaseolus Vulgaris ◽  
Pseudomonas Syringae ◽  
Time Course ◽  
Early Time ◽  
Experimental System ◽  
Antioxidant Response ◽  
Defense Responses ◽  
Cellular Damage ◽  
Initial Increase ◽  
Phaseolus Vulgaris L

Common bean (Phaseolus vulgaris L.) is attacked by several pathogens such as the biotrophic gamma-proteobacterium Pseudomonas syringae pv. phaseolicola (Pph). In order to study the Pph-bean interaction during the first stages of infection, leaf disks of a susceptible bean variety named Riñón were infected with a pathogenic Pph. Using this experimental system, six new putative Wall-Associated Kinase (WAKs) receptors, previously identified in silico, were tested. These six bean WAKs (PvWAKs) showed high protein sequence homology to the well-described Arabidopsis WAK1 (AtWAK1) receptor and, by phylogenetic analysis, clustered together with AtWAKs. The expression of PvWAK1 increased at very early stages after the Pph infection. Time course experiments were performed to evaluate the accumulation of apoplastic H2O2, Ca2+ influx, total H2O2, antioxidant enzymatic activities, lipid peroxidation, and the concentrations of abscisic acid (ABA) and salicylic acid (SA), as well as the expression of six defense-related genes – MEKK-1, MAPKK, WRKY33, RIN4, PR1 and NPR1. The results showed that overexpression of PR1 occurred 2 h after Pph infection without a concomitant increase in SA levels. Although apoplastic H2O2 increased after infection, the oxidative burst was neither intense nor rapid and an efficient antioxidant response did not occur, suggesting that the observed cellular damage was due to the initial increase in total H2O2 at early time points after infection. In conclusion, the Riñón variety can perceive the presence of Pph, but this recognition only results in a modest and slow activation of host defenses, leading to high susceptibility to Pph.

scholarly journals Soil Application of Effective Microorganisms (EM) Maintains Leaf Photosynthetic Efficiency, Increases Seed Yield and Quality Traits of Bean (Phaseolus vulgaris L.) Plants Grown on Different Substrates

2019 ◽  
Vol 20 (9) ◽  
pp. 2327 ◽  
Author(s):  
Marcello Iriti ◽  
Alessio Scarafoni ◽  
Simon Pierce ◽  
Giulia Castorina ◽  
Sara Vitalini
Keyword(s):  
Phaseolus Vulgaris ◽  
Photosystem Ii ◽  
Sandy Soil ◽  
Photosynthetic Efficiency ◽  
Quality Traits ◽  
Phaseolus Vulgaris L ◽  
Effective Microorganisms ◽  
Yield And Quality ◽  
Bean Plants

EM (effective microorganisms) is a biofertilizer consisting of a mixed culture of potentially beneficial microorganisms. In this study, we investigated the effects of EM treatment on leaf in vivo chlorophyll a fluorescence of photosystem II (PSII), yield, and macronutrient content of bean plants grown on different substrates (nutrient rich substrate vs. nutrient poor sandy soil) in controlled environmental conditions (pot experiment in greenhouse). EM-treated plants maintained optimum leaf photosynthetic efficiency two weeks longer than the control plants, and increased yield independent of substrate. The levels of seed nutritionally-relevant molecules (proteins, lipids, and starch) were only slightly modified, apart from the protein content, which increased in plants grown in sandy soil. Although EM can be considered a promising and environmentally friendly technology for sustainable agriculture, more studies are needed to elucidate the mechanism(s) of action of EM, as well as its efficacy under open field conditions.

scholarly journals Investigations on the pathogenicity of Helminthosporium sorokinianum Sacc. in relation to Phaseolus vulgaris L.

2014 ◽  
Vol 10 (2) ◽  
pp. 283-293
Author(s):  
Barbara Łacicowa ◽  
Zofia Machowicz
Keyword(s):  
Phaseolus Vulgaris ◽  
Root System ◽  
Field Experiments ◽  
Phaseolus Vulgaris L ◽  
Bean Plants ◽  
Before And After

The results obtained in pot and field experiments have shown that <i>Helminthosporium sorokinianum</i> is able to infect bean plants. The cotyledons and roots of shoots during the first three weeks of growth are attacked the most frequently. Dark brown spots occur on the above-mentined organs. The infection of roots and cotyledonsof shoots is responsible for gangrene both before and after germination. Infected plants which remain alive only show symptoms of infection in the root system. The infection of roots by <i>H. sorokinianum</i> in older plants is detromental to growth and causes a decrease in the vield obtained from bean plants.

Studies on phytocides. I. Diurnal variation in effectiveness of 'methoxone'

1950 ◽  
Vol 1 (2) ◽  
pp. 148 ◽  
Author(s):  
CG Greenham
Keyword(s):  
Phaseolus Vulgaris ◽  
Diurnal Variation ◽  
Phaseolus Vulgaris L ◽  
Bean Plants ◽  
Spray Applications ◽  
Chondrilla Juncea

As determined by the length for which roots were killed, there was no diurnal variation in the effectiveness on skeleton weed (Chondrilla juncea L.) of spray applications of 'Methoxone' at two-hourly intervals over a period of 24 hours. As determined by reduction in growth above the primary leaves, there was a well-marked diurnal variation in the effectiveness on bean plants (Phaseolus vulgaris L.) of drop applications of 'Methoxone.'

Sign in / Sign up

Export Citation Format

Share Document