Thidiazuron Induces High-Frequency Shoot Regeneration in Intact Seedlings of Pea (Pisum sativum), Chickpea (Cicer arietinum) and Lentil (Lens culinaris)

1992 ◽  
Vol 19 (6) ◽  
pp. 731 ◽  
Author(s):  
KA Malik ◽  
PK Saxena
Keyword(s):  
Pisum Sativum ◽  
Cicer Arietinum ◽  
Lens Culinaris ◽  
De Novo ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Grain Legumes ◽  
Genotypic Difference ◽  
Vascular Connection ◽  
Shoot Buds

Axenic seedling cultures of chickpea (Cicer arietinum L.), lentil (Lens culinaris Medik.) and garden pea (Pisum sativum L.) were established by culturing mature seeds on Murashige and Skoog medium (MS) supplemented with thidiazuron (TDZ). Of various cytokinins or compounds with cytokinin-like activity (Kinetin, TDZ, Zeatin) tested for inducing shoot formation in pea seeds cultures, TDZ was found to be most effective. Pea seedlings exhibited a unique pattern of shoot formation which was accomplished in two distinct phases. Multiple shoots developed within a week, from the nodal and basal regions of the primary epicotyl in a medium that contained 5-50 μM TDZ. When these seedlings were exposed for a prolonged time period (3-4 weeks), to the same medium, numerous shoot buds emerged de novo from the base and/or from the upper part of multiple shoots. These shoots had no apparent vascular connection with parent tissues. The inductive capability of TDZ was then tested in several other genotypes of Pisum sativum and two other large-seeded grain legumes, Cicer arietinum, and Lens culinaris. In Cicer arietinum, and Lens culinaris, multiple shoots developed after 1 week of seed culture on media that contained 1-50 μM TDZ. However, de novo differentiation of shoot buds occurred in cultures exposed to TDZ for 4-6 weeks, only from nodal and subjacent areas. Secondary shoot formation occurred frequently in all of the species tested. Developing shoots were able to form roots and eventually whole plants on a modified MS medium containing 2.5 μM NAA. No genotypic difference for morphogenesis was observed.

scholarly journals Development and Use of Core Subsets of Cool-season Food Legume Germplasm Collections

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 907C-907 ◽  
Author(s):  
Charles J. Simon ◽  
Richard M. Hannan
Keyword(s):  
Pisum Sativum ◽  
Cicer Arietinum ◽  
Entire Range ◽  
Lens Culinaris ◽  
Germplasm Evaluation ◽  
Cool Season ◽  
Logarithmic Model ◽  
Adaptation Potential ◽  
Germplasm Collections ◽  
Food Legume

Core subsets have been selected for the USDA chickpea (Cicer arietinum), lentil (Lens culinaris), and pea (Pisum sativum) germplasm collections. These subsets are specifically intended to increase the efficiency of the utilization of the entire collections of these taxa. The cores consist of 13% of the 3873 chickpeas, 12% of the 2390 lentils, and 17.5% of the 2886 pea accessions. They were selected by a proportional logarithmic model, and also contain additional accessions based upon documented concentrations of diversity. Each core has been screened for disease reactions, and results suggest that the cores can effectively direct germplasm users toward portions of the entire collections that contain resistant germplasm. These cores have also been useful for those interested in assessing the adaptation potential of these crops in new environments, because the entire range of adaptation is represented. Although cores may not always enhance access to germplasm with unique or extremely rare characteristics, the legume cores have been very useful for directing users toward desirable germplasm from defined geographic areas, and assisting users at the preliminary stages of germplasm evaluation.

Identification of cystathionine γ-synthase and threonine synthase from Cicer arietinum and Lens culinaris

2013 ◽  
Vol 91 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Dominique J.K. Morneau ◽  
Allison F. Jaworski ◽  
Susan M. Aitken
Keyword(s):  
Cicer Arietinum ◽  
Lens Culinaris ◽  
Amino Acid Sequence Identity ◽  
Grain Legumes ◽  
Model Organisms ◽  
Grain Legume ◽  
Target Species ◽  
Active Site Residues ◽  
Threonine Synthase ◽  
Sequence Identity

In plants, cystathionine γ-synthase (CGS) and threonine synthase (TS) compete for the branch-point metabolite O-phospho-l-homoserine. These enzymes are potential targets for metabolic engineering studies, aiming to alter the flux through the competing methionine and threonine biosynthetic pathways, with the goal of increasing methionine production. Although CGS and TS have been characterized in the model organisms Escherichia coli and Arabidopsis thaliana, little information is available on these enzymes in other, particularly plant, species. The functional CGS and TS coding sequences from the grain legumes Cicer arietinum (chickpea) and Lens culinaris (lentil) identified in this study share approximately 80% amino acid sequence identity with the corresponding sequences from Glycine max. At least 7 active-site residues of grain legume CGS and TS are conserved in the model bacterial enzymes, including the catalytic base. Putative processing sites that remove the targeting sequence and result in functional TS were identified in the target species.

Heterodera ciceri. [Distribution map].

2003 ◽  
Author(s):  
Keyword(s):  
Pisum Sativum ◽  
Cicer Arietinum ◽  
Geographical Distribution ◽  
Lens Culinaris ◽  
Distribution Map ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Heterodera ciceri Vovlas et al. Nematoda: Heteroderidae Hosts: Chickpea (Cicer arietinum) and lentil (Lens culinaris), also pea (Pisum sativum). Information is given on the geographical distribution in EUROPE, Italy, Spain, ASIA, Jordan, Lebanon, Syria, Turkey.

Pea and soybean performance in Newfoundland

2001 ◽  
Vol 81 (4) ◽  
pp. 723-726 ◽  
Author(s):  
D. Spaner ◽  
A. G. Todd ◽  
D. B. McKenzie
Keyword(s):  
Glycine Max ◽  
Pisum Sativum ◽  
Grain Legumes ◽  
Yield Potential ◽  
Nodule Formation ◽  
Soil Temperatures ◽  
Faba Beans ◽  
Glycine Max L ◽  
Livestock Farmers ◽  
Near Future

Newfoundland livestock farmers import all feed grain legumes. Our objective was to compare field pea (Pisum sativum L.) and soybean [Glycine max (L.) Merr.] nodule formation, plant growth, grain and protein yield potential in Newfoundland. Two-year experimental mean pea yield was high (≈0.3 t grain ha–1), although lodging was severe. Vision soybean yielded around 400 kg grain protein ha–1 when seeds were inoculated, even with soil temperatures below 20°C. Despite reasonable yield potentials, agronomic adaptation problems mitigate against the local production of all common feed grain legumes in the near future. Key words: Pisum sativum; Glycine max; lupins; faba beans; inoculant

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

2011 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Suttinee JINGJIT ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
In Vitro Flowering ◽  
Nodal Explants ◽  
Callus Growth ◽  
Ms Medium ◽  
Old Field ◽  
Gypsophila Paniculata ◽  
Initial Culture

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

Sign in / Sign up

Export Citation Format

Share Document