NMR study of low subcellular pH during the development of cherry tomato fruit

2000 ◽  
Vol 27 (1) ◽  
pp. 61 ◽  
Author(s):  
Dominique Rolin ◽  
Pierre Baldet ◽  
Daniel Just ◽  
Christian Chevalier ◽  
Marc Biran ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Organic Acids ◽  
Fruit Development ◽  
Tomato Fruit ◽  
31P Nmr Spectroscopy ◽  
Cytoplasmic Ph ◽  
Cherry Tomato ◽  
Ph Changes ◽  
Nmr Study

Changes in metabolites (organic acids, sugars and amino acids) and subcellular pH were studied during fruit development of cherry tomato (Lycopersicon esculentum Mill. var. cerasiformae). Fructose and glucose were the major sugars, whereas citrate and malate the two major organic acids. At different stages of fruit development, vacuolar and cytoplasmic pH changes were followed by in vivo 13C and 31P NMR spectroscopy. Fruit compartments had a cytoplasmic pH around 7.1 as early as the cell-divi-sion and -expansion stages. The vacuolar pH measured by in vivo 13C NMR spectroscopy decreased from 4.5 to 3.6. Concomitantly, strong accumulation of γ-aminobutyric acid (GABA) was observed during the first 15 days after anthesis and glutamate decarboxylase (GAD) activity increased 10-fold during the first 8 days of development. The relationships between organic acid biosynthesis and storage, GABA produc-tion, and subcellular pH changes during development of cherry tomato fruit are discussed.

31P-NMR spectroscopy of isolated perfused rat lung

1993 ◽  
Vol 74 (4) ◽  
pp. 1549-1554 ◽  
Author(s):  
Y. Hayashi ◽  
T. Inubushi ◽  
S. Nioka ◽  
R. E. Forster
Keyword(s):  
Nmr Spectroscopy ◽  
Intracellular Ph ◽  
31P Nmr ◽  
Peak Height ◽  
31P Nmr Spectroscopy ◽  
Line Broadening ◽  
Membrane Phospholipids ◽  
The Difference ◽  
Inflated Lung

We obtained 202.5-MHz 31P-nuclear magnetic resonance (NMR) spectra of isolated perfused rat lungs, degassed and inflated, and of lung extract. The spectra included those of ATP, ADP, phosphocreatine (PCr), inorganic phosphate (Pi), phosphomonoesters, phosphodiesters, and a broad component due to the membrane phospholipids. The line width at one-half peak height for beta-ATP was 1.0 ppm for the degassed lung and 1.2 ppm for the inflated lung. This suggests that the air-water interfaces in inflated lung, which produce proton NMR line broadening, do not act prominently in 31P-NMR spectroscopy. In a degassed lung, when perfusion was stopped for up to 30 min, PCr and ATP peaks decreased progressively with time while Pi and phosphomonoester peaks increased. On return of flow, these changes reversed. The intracellular pH values calculated from the difference in magnetic field between PCr and Pi peaks of inflated and degassed lungs were 7.16 +/- 0.10 (SD; n = 4) and 6.99 +/- 0.10 (n = 4), respectively. The change of intracellular pH caused by 30 min of ischemia was -0.2 pH units. Our findings indicate that air-water interfaces should not broaden lung 31P peaks in vivo.

NMR study of low subcellular pH during the development of cherry tomato fruit

2000 ◽  
Vol 13 (5) ◽  
Author(s):  
Dominique Rolin ◽  
Pierre Baldet ◽  
Daniel Just ◽  
Christian Chevalier ◽  
Marc Biran ◽  
...  
Keyword(s):  
Tomato Fruit ◽  
Cherry Tomato ◽  
Nmr Study

31P NMR spectroscopy of in vivo tumors

1984 ◽  
Vol 2 (1) ◽  
pp. 70-71
Author(s):  
T.C. Ng ◽  
W.T. Evanochko ◽  
R.N. Hiramoto ◽  
V.K. Ghanta ◽  
M.B. Lilly ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
31P Nmr Spectroscopy

Partial cerebral ischemia assessed by “in vivo” 31P NMR spectroscopy in rats

1992 ◽  
Vol 10 (5) ◽  
pp. 769-772 ◽  
Author(s):  
M.A. Macrì ◽  
R. Campanella ◽  
G. Garreffa ◽  
M. Occhigrossi ◽  
F. De Luca ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Nmr Spectroscopy ◽  
31P Nmr ◽  
31P Nmr Spectroscopy

Development of fruit cuticle in cherry tomato (Solanum lycopersicum)

2008 ◽  
Vol 35 (5) ◽  
pp. 403 ◽  
Author(s):  
Eva Domínguez ◽  
Gloria López-Casado ◽  
Jesús Cuartero ◽  
Antonio Heredia
Keyword(s):  
Fruit Development ◽  
Tomato Fruit ◽  
Significant Loss ◽  
Fruit Growth ◽  
Epidermal Differentiation ◽  
Cherry Tomato ◽  
Cellulose Material ◽  
Cuticle Formation ◽  
Fruit Development And Ripening ◽  
Cuticle Thickness

The cuticle of a plant plays an important role in many physiological events of fruit development and ripening. Despite this, little is known about cuticle formation and development. We include a detailed morphological study at the microscopic level of cuticle during fruit growth and ripening using tomato as a fruit model. In addition, a study of the differences in cuticle thickness and composition during development is included. The four genotypes studied in this work showed a similar timing of the main morphological events: initiation of epidermal differentiation, changes in the distribution of the lipid, pectin and cellulose material within the cuticle, appearance of pegs, beginning of cuticle invaginations, maximum thickness and loss of polysaccharidic material. Fruit growth, measured by fruit diameter, showed a positive correlation with the increase of cuticle thickness and the amount of cuticle and their cutin and polysaccharide components per fruit unit during development. By contrast, cuticle waxes showed a different behaviour. Two important characteristics of cuticle growth were observed during tomato fruit development. First, the amount of cuticle per surface area reached its maximum in the first 15 days after anthesis and remained more or less constant until ripening. Second, there was a significant loss of polysaccharidic material from the beginning of ripening (breaker stage) to full red ripe.

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