Sucrose Accumulation in Red Beet Vacuoles by UDP Glucose-Dependent Group Translocation — Fact or Artefact?

1988 ◽  
Vol 15 (3) ◽  
pp. 359 ◽  
Author(s):  
C Niemietz ◽  
JS Hawker
Keyword(s):  
Sucrose Accumulation ◽  
Sucrose Transport ◽  
Chromatographic Separations ◽  
Glucan Synthase ◽  
Red Beet ◽  
Translocation Mechanism ◽  
Insoluble Product ◽  
Group Translocation

Beetroot vacuoles obtained by slicing or enzymic techniques incorporated UDP[14C] glucose predominantly into an ethanol insoluble product. No labelled sucrose was detected. Chromatographic separations and kinetic experiments suggested the results were due to glucan synthase II (EC 2.4.1.34). Evidence is presented that this enzyme can account for many of the features previously attributed to the proposed UDPglucose-dependent group translocation mechanism for sucrose transport into red beet vacuoles. The possible interference by glucan synthase II in experiments published previously is discussed.

scholarly journals Immunological Evidence for the Existence of a Carrier Protein for Sucrose Transport in Tonoplast Vesicles from Red Beet (Beta vulgaris L.) Root Storage Tissue

1993 ◽  
Vol 102 (3) ◽  
pp. 751-760 ◽  
Author(s):  
H. P. Getz ◽  
J. Grosclaude ◽  
A. Kurkdjian ◽  
F. Lelievre ◽  
A. Maretzki ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Carrier Protein ◽  
Sucrose Transport ◽  
Storage Tissue ◽  
Red Beet

Inactivation of red beet β-glucan synthase by native and oxidized phenolic compounds

1987 ◽  
Vol 26 (8) ◽  
pp. 2197-2202 ◽  
Author(s):  
Theresa L. Mason ◽  
Wasserman Bruce P.
Keyword(s):  
Phenolic Compounds ◽  
Glucan Synthase ◽  
Red Beet

scholarly journals Regulation of Plasma Membrane β-Glucan Synthase from Red Beet Root by Phospholipids

1986 ◽  
Vol 82 (2) ◽  
pp. 396-400 ◽  
Author(s):  
Bruce P. Wasserman ◽  
Kevin J. McCarthy
Keyword(s):  
Plasma Membrane ◽  
Glucan Synthase ◽  
Beet Root ◽  
Red Beet

scholarly journals CHAPS Solubilization and Functional Reconstitution of β-Glucan Synthase from Red Beet Root (Beta vulgaris L.) Storage Tissue

1987 ◽  
Vol 85 (2) ◽  
pp. 516-522 ◽  
Author(s):  
Margaret E. Sloan ◽  
Panayotis Rodis ◽  
Bruce P. Wasserman
Keyword(s):  
Beta Vulgaris ◽  
Storage Tissue ◽  
Glucan Synthase ◽  
Beet Root ◽  
Red Beet

scholarly journals Partial Purification of Digitonin-Solubilized β-Glucan Synthase from Red Beet Root

1987 ◽  
Vol 83 (4) ◽  
pp. 982-987 ◽  
Author(s):  
Laura L. Eiberger ◽  
Bruce P. Wasserman
Keyword(s):  
Partial Purification ◽  
Glucan Synthase ◽  
Beet Root ◽  
Red Beet

scholarly journals Developmental Transition Between Sucrose Uptake and Sucrose Efflux Systems in the Vacuole of Red Beet Hypocotyl Cells

HortScience ◽  
2001 ◽  
Vol 36 (7) ◽  
pp. 1300-1301 ◽  
Author(s):  
Ed Etxeberria ◽  
Pedro Gonzalez
Keyword(s):  
Concentration Gradient ◽  
Developmental Stages ◽  
Developmental State ◽  
Sucrose Accumulation ◽  
Sucrose Uptake ◽  
Physiological Mechanisms ◽  
Developmental Transition ◽  
Red Beet

The mechanisms of sucrose uptake into the vacuole and sucrose efflux from the vacuole were studied using tonoplast vesicles from red beet at two distinct developmental stages. Vesicles from both developing and mobilizing hypocotyls (sucrose uptake and efflux, respectively) accumulated sucrose against a concentration gradient. However, higher rates and maximal levels of sucrose accumulation were obtained with tonoplast from developing hypocotyls. ATP-dependent sucrose efflux was more pronounced in vesicles from mobilizing hypocotyls. Despite the apparent overlapping, the data indicate that the physiological mechanisms for sucrose uptake and sucrose efflux are separated in time and governed by the developmental state of the cell. Chemical name used: adenosine 5′-triphosphate (ATP).

scholarly journals Exogenous Application of Sucrose Promotes Postharvest Ripening of Kiwifruit

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 245 ◽  
Author(s):  
Liuying Fei ◽  
Xin Yuan ◽  
Chuying Chen ◽  
Chunpeng Wan ◽  
Yongqi Fu ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Sucrose Accumulation ◽  
Ethylene Synthesis ◽  
Sucrose Transport ◽  
Ripening Process ◽  
Ethylene Content ◽  
Sugar Signal ◽  
Postharvest Ripening ◽  
Fruit Flavor ◽  
Ethylene Signal

Sucrose is an important component of fruit flavor, but whether sucrose signaling affects the postharvest ripening process of kiwifruit is unclear. The aim of this article was to study the effect of sucrose application on postharvest kiwifruit ripening to clarify the effect of sucrose in this process. Our present study found that exogenous sucrose can promote ethylene synthesis, which increases the ethylene content during fruit ripening, thereby accelerating the ripening and softening of kiwifruit after harvest. A significantly higher expression of AcACS1 and AcACO2 was found in sucrose-treated fruits compared to that in mannitol-treated fruits. Blocking the ethylene signal significantly inhibited the sucrose-modulated expression of most selected ripening-related genes. Sucrose transport is essential for sucrose accumulation in fruits; therefore, we isolated the gene family related to sucrose transport in kiwifruit and analyzed the gene expression of its members. The results show that AcSUT1 and AcTST1 expression increased with fruit ripening and AcSUT4 expression decreased with ripening, indicating that they may have different roles in the regulation of fruit ripening. Additionally, many cis-elements associated with phytohormones and sugar responses were found in the promoter of the three genes, which suggests that they were transcriptionally regulated by sugar signal and phytohormones. This study demonstrates the effect of sucrose on postharvest ripening of kiwifruit, providing a good foundation for further research.

Solubilization of a digitonin-stable glucan synthase from red beet root

1985 ◽  
Vol 37 (3) ◽  
pp. 195-198 ◽  
Author(s):  
Laura L. Eiberger ◽  
Carol L. Ventola ◽  
Bruce P. Wasserman
Keyword(s):  
Glucan Synthase ◽  
Beet Root ◽  
Red Beet

Temporal and Spatial Regulation of Sucrose Accumulation in the Sugarcane Stem

1995 ◽  
Vol 22 (4) ◽  
pp. 661 ◽  
Author(s):  
PH Moore
Keyword(s):  
Dynamic Model ◽  
Sucrose Synthesis ◽  
Sucrose Accumulation ◽  
Rapid Cycling ◽  
Sucrose Transport ◽  
Spatial Regulation ◽  
Cells In Culture ◽  
Molecular Studies ◽  
Temporal And Spatial ◽  
Sugarcane Stem

Sucrose accumulation has been studied extensively in sugarcane-an example of a highly productive crop plant with the capacity for storing large quantities of sugar. Initial recognition and characterisation of the enzymes involved in sucrose synthesis and cleavage led to widely accepted models of how sucrose transport and accumulation occur. Studies on cells in culture and on isolated cellular fragments initially supported and strengthened these models but more recently have revealed weaknesses in them. Biophysical measurements and anatomical, histochemical, and tracer dye studies further eroded the older models. Molecular studies are beginning to reveal details at the gene and transcriptional levels of the enzymes involved in sucrose transport and metabolism. Collectively, results of recent research indicate the need for a new sucrose accumulation model. A dynamic model of rapid cycling and turnover of sucrose between the vacuole and metabolic and apoplastic compartments explains much of the data, but details of how the cycling is regulated remains to be discovered.

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