The maize transcription factor Sn alters proanthocyanidin synthesis in transgenic Lotus corniculatus plants

1999 ◽  
Vol 26 (2) ◽  
pp. 159 ◽  
Author(s):  
Francesco Damiani ◽  
Francesco Paolocci ◽  
Paul D. Cluster ◽  
Sergio Arcioni ◽  
Gregory J. Tanner ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Binary Vector ◽  
Regulatory Gene ◽  
Lotus Corniculatus ◽  
35S Promoter ◽  
Gus Gene ◽  
Plant Leaves ◽  
Negative Interaction ◽  
Transformed Plants ◽  
And Control

Lotus corniculatus L. plants were transformed with Agrobacterium rhizogenes binary vector carrying the maize Sn regulatory gene driven by the 35S promoter. These plants showed modifications in the pattern of accumulation of proanthocyanidin (PA). All the transformed plants but one showed an increase in PA content in the root relative to control untransformed and control gus gene transformed plants (C). With respect to the PAaccumulation in leaves, Sn transgenic plants were grouped in two classes: suppressed (S), that showed a consistent reduction of foliar PAcontent, and unsuppressed (U) that did not differ significantly from controls. Dihydroflavanol reductase (DFR) and leucocyanidin reductase (LAR) enzyme activities in S and U plant leaves mirrored the changes seen with foliar PA accumulation. LAR activity in the roots was consistent with the root PA levels. Mature Sn mRNA accumulated in the leaves of U plants, but not in leaves of S plants; however, leaves of both S and U plants were able to initiate Sn transcription. All Sn-transformed plants accumulated Sn message in root tissue. A possible negative interaction of Sn and an unidentified homologous endogene is proposed for explaining the behaviour of S plants.

scholarly journals INTRODUCTION OF β-GLUCURONIDASE GENE INTO GINSENG (PANAX CINSENG) BY A TI-PLASMID VECTOR SYSTEM AND ITS EXPRESSION

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 621e-621
Author(s):  
Jang R. Liu ◽  
Haeng S. Lee ◽  
Suk W. Kim ◽  
Hyo W. Lee
Keyword(s):  
Somatic Embryos ◽  
Binary Vector ◽  
Zygotic Embryo ◽  
Plasmid Vector ◽  
Vector System ◽  
Selection Marker ◽  
35S Promoter ◽  
Dichlorophenoxyacetic Acid ◽  
Gus Gene ◽  
2,4 Dichlorophenoxyacetic Acid

β-Glucuronidase (GUS) gene of Escherichia coli was introduced into ginseng cells by an Agrobacterium binary vector system and expressed in somatic embryos derived from the cells. A binary vector pBI121 carrying CaMV 35S promoter-GUS gene fusion and a neomycin phosphotransferase gene as selection marker was transferred into Agrobacterium tumefaciens LBA4404. Zygotic embryo cotyledonary segments were co-cultivated with A. tumefaciens and transferred to the medium containg 1 mg 2,4-dichlorophenoxyacetic acid/liter, 0.5 mg kinetin/liter, and 100 mg kanamycin/liter. Kanamycin-resistant calli were formed after 3 to 4 weeks of culture. Southern analysis confirmed the resistant calli were transformed with GUS gene. High GUS activities were detected in somatic embryos developed from the calli.

Antipyretic activity of the root extracts of Desmodium Gangeticum

2018 ◽  
Vol 8 (2) ◽  
pp. 20-23
Author(s):  
Raja Sheker K ◽  
Naveen B ◽  
Anil kumar A ◽  
Abhilash G
Keyword(s):  
Gastric Ulcers ◽  
Plant Leaves ◽  
Medical Systems ◽  
Human Race ◽  
Standard Drug ◽  
Root Extracts ◽  
Antipyretic Activity ◽  
Scientific Investigations ◽  
And Control ◽  
Effective Drugs

Fevers are considered as the most important parameters to evaluate and diagnose most of the disease conditions like inflammations, wounds and other infections. There are effective drugs that treat and control the fevers out of which NSAID's are most important ones. They cause notable side effects like gastric ulcers, gastric mucosal perforations etc. which make the use of those drugs limited. Herbs are used to treat various diseases, starting from the evolution of the human race. During this, herbs had been introduced to many types of tests and scientific investigations to prove the activities that herbs possess. The diseases that the herbs are used for are notable in the medical systems like Ayurveda and other systems. The need for the validation of the activities of the herbs and medicinal plants is utmost important these days. The extracts of the plant leaves of Desmodium gangeticum were extracted with ethanol and then investigated for the antipyretic activity in yeast induced pyretic method. The extract was tested in two doses 200 and 400mg/kg. This was found significant when compared to the standard drug.

Bioinspired photo-responsive membrane enhanced with the “light-cleaning” feature for controlled molecule release

2022 ◽  
Author(s):  
Qisheng Ye ◽  
Rui Wang ◽  
Saitao Yan ◽  
Baoliang Chen ◽  
Xiaoying Zhu
Keyword(s):  
Membrane Fouling ◽  
Plant Leaves ◽  
Responsive Polymers ◽  
And Control

Inspired by the stomatal of plant leaves, a photo-responsive membrane was developed to enhance the removal of irreversible membrane fouling and control molecule release. The photo-responsive polymers were prepared by...

scholarly journals Altered Glut-2 accumulation and β-cell function in mice lacking the exocrine-specific transcription factor, Mist1

2005 ◽  
Vol 187 (3) ◽  
pp. 407-418 ◽  
Author(s):  
E N Fazio ◽  
M Everest ◽  
R Colman ◽  
R Wang ◽  
C L Pin
Keyword(s):  
Transcription Factor ◽  
Glucose Tolerance ◽  
Cell Function ◽  
Regulatory Gene ◽  
Immunohistochemical Analysis ◽  
Endocrine Function ◽  
Pancreatic Acinar Cells ◽  
Protein Accumulation ◽  
Specific Transcription Factor ◽  
And Function

Mist1 is an exocrine-specific transcription factor that is necessary for the establishment of cell organization and function of pancreatic acinar cells. While Mist1 is not expressed in the endocrine pancreas, the disorganized phenotype of the exocrine component may affect endocrine function. Therefore, we examined endocrine tissue morphology and function in Mist1-knockout (Mist1KO) mice. Endocrine function was evaluated using a glucose-tolerance test on 2–10-month-old female mice and revealed a significant reduction in glucose-clearing ability in 10-month-old Mist1KO mice compared with wild-type mice. Immunohistochemical analysis of islet hormone expression indicated that the decreased endocrine function was not due to a decrease in insulin-, glucagon- or somatostatin-expressing cells. However, a decrease in the size of islets in 10-month-old Mist1KO mice was observed along with a decrease in Glut-2 protein accumulation. These results suggest that the islets in Mist1KO mice are functionally compromised, likely accounting for the decreased glucose tolerance. Based on these findings, we have identified that the loss of a regulatory gene in the exocrine compartment can affect the endocrine component, providing a possible link between susceptibility for various pancreatic diseases.

scholarly journals Differential timing of a conserved transcriptional network underlies divergent cortical projection routes across mammalian brain evolution

2020 ◽  
Vol 117 (19) ◽  
pp. 10554-10564 ◽  
Author(s):  
Annalisa Paolino ◽  
Laura R. Fenlon ◽  
Peter Kozulin ◽  
Elizabeth Haines ◽  
Jonathan W. C. Lim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Corpus Callosum ◽  
Anterior Commissure ◽  
Regulatory Gene ◽  
Brain Evolution ◽  
Projection Neuron ◽  
Egg Laying ◽  
Mammalian Brain ◽  
Transcriptional Networks ◽  
Cortical Projection

A unique combination of transcription factor expression and projection neuron identity demarcates each layer of the cerebral cortex. During mouse and human cortical development, the transcription factor CTIP2 specifies neurons that project subcerebrally, while SATB2 specifies neuronal projections via the corpus callosum, a large axon tract connecting the two neocortical hemispheres that emerged exclusively in eutherian mammals. Marsupials comprise the sister taxon of eutherians but do not have a corpus callosum; their intercortical commissural neurons instead project via the anterior commissure, similar to egg-laying monotreme mammals. It remains unknown whether divergent transcriptional networks underlie these cortical wiring differences. Here, we combine birth-dating analysis, retrograde tracing, gene overexpression and knockdown, and axonal quantification to compare the functions of CTIP2 and SATB2 in neocortical development, between the eutherian mouse and the marsupial fat-tailed dunnart. We demonstrate a striking degree of structural and functional homology, whereby CTIP2 or SATB2 of either species is sufficient to promote a subcerebral or commissural fate, respectively. Remarkably, we reveal a substantial delay in the onset of developmental SATB2 expression in mice as compared to the equivalent stage in dunnarts, with premature SATB2 overexpression in mice to match that of dunnarts resulting in a marsupial-like projection fate via the anterior commissure. Our results suggest that small alterations in the timing of regulatory gene expression may underlie interspecies differences in neuronal projection fate specification.

scholarly journals Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor

Cell ◽  
1984 ◽  
Vol 38 (1) ◽  
pp. 175-182 ◽  
Author(s):  
Robert Landick ◽  
Vicki Vaughn ◽  
Elizabeth T. Lau ◽  
Ruth A. VanBogelen ◽  
James W. Erickson ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Nucleotide Sequence ◽  
Regulatory Gene ◽  
Protein Product ◽  
E Coli

Anthocyanin regulatory gene expression in transgenic white clover can result in an altered pattern of pigmentation

2000 ◽  
Vol 27 (7) ◽  
pp. 659
Author(s):  
John de Majnik ◽  
Jeremy J. Weinman ◽  
Michael A. Djordjevic ◽  
Barry G. Rolfe ◽  
Greg J. Tanner ◽  
...  
Keyword(s):  
White Clover ◽  
Regulatory Gene ◽  
Phenylpropanoid Pathway ◽  
Regulatory Genes ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Anthocyanin Accumulation ◽  
Cauliflower Mosaic Virus 35S ◽  
White Clover Plant ◽  
Clover Plant

This study presents the first evidence of heterologous anthocyanin regulatory genes altering anthocyanin expression in stably transformed leguminous plants. Two families of anthocyanin regulatory genes, myc (delila, B-Peru) and myb (myb.Ph2, C1), are involved in the activation of the phenylpropanoid pathway. White clover (Trifolium repens cv. Haifa) plants were transformed with dicotyledonous and monocotyledonous myb or myc genes. Some of these transformed plants exhibited enhanced anthocyanin accumulation in a range of tissues. One plant, transformed with the B-Peru gene driven by the Cauliflower Mosaic Virus 35S promoter, displayed a unique pattern of anthocyanin accumulation in the leaf. The accumulation of anthocyanin in this plant was closely associated with the crescent of leaves, which is normally white. The red pigmentation declined in intensity in the oldest leaf stage. The B-Peru message was detected in all leaf stages of this white clover plant. This anthocyanin pattern was shown to be heritable.

scholarly journals Successful genetic transformation in date palm (Phoenix dactylifera)

2014 ◽  
Vol 11 (2) ◽  
pp. 171-176 ◽  
Author(s):  
L Hassan
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Agrobacterium Rhizogenes ◽  
Reporter Gene ◽  
Hairy Roots ◽  
Date Palm ◽  
Binary Vector ◽  
Nptii Gene ◽  
Neomycin Phosphotransferase ◽  
Gus Gene ◽  
Nos Terminator

The introduction of foreign genes into most of the Phoenix spp using recombinant DNA technology is not a straight forward task. In Phoenix spp application of this technology towards successful transformation proved to be a more difficult one – so far no report on the successful regeneration of transgenic date palm plants has been published. We developed an efficient and reproducible variety-independent method for producing transgenic date palm (Phoenix spp) via Agrobacterium-mediated transformation. Agrobacterium rhizogenes strains LBA 9402 were used and for cotransformation experiments the strain LBA 9402 with the binary vector pBIN19 with the p35S GUS INT gene was used. Off-shoot segments from different Phoenix spp cultivars were infected with Agrobacterium rhizogenes. The development of ‘hairy roots’ at a high frequency only on infected tissue pieces showed that transformation is possible. Various parameters like, effect of different genotypes on root initiation, root number and root length have been studied. Regeneration of transformed root cultures to plantlets was also attempted. Histochemical GUS assay and polymerase chain reaction analysis of hairy roots confirmed the presence of GUS gene. Agrobacterium tumifaciensmediated transformation was also performed using the leaves of off-shoot explants. Agrobacterium tumefaciens strains: I) GV3101 with the vir plasmid pMP90 the strain C58C1 ATHV with the vir-plasmid pTiBo542 (=pEHA101; Hood et al. 1986) was used. The nptII gene (neomycin phosphotransferase) was used as a selectable marker gene. The ?-Glucuronidase-gene (GUS-Gene: Jefferson et al. 1987) under control of the Ubi- and 35S-Promotors, with an Intron (Vancanneyt et al. 1990), was used as the reporter gene. We also used the genetically engineered Agrobacterium tumefaciens strain LBA4404 as a vector for infection in the transformation experiment, which contains plasmid pBI121 of 14 KDa (binary vector). This binary vector contains following genes within the right border (RB) and left border (LB) region of the construct: The udiA gene (Jefferson, 1986) predetermining GUS (?-glucuronidase), driven by CaMV promoter and NOS terminator. This reporter gene can be used to assess the efficiency of transformation. The nptII gene (Herrera-Estrella et al., 1983) encoding neomycin phosphotransferase II (nptII) conferring kanamycin resistance, driven by NOS promoter and NOS terminator. The bacterium also contains plasmid pAL4404 which is a disarmed Ti plasmid (132 KDa) containing the virulence genes. For the confirmation of transgenes, calli were taken from the growing callus mass for DNA isolation. PCR- and Southern analysis was performed to determine the integration and the copy number of the transgene. The GUS-test was performed to demonstrate ß-glucuronidase expression. The transgenic plantlets were kept in a hardening room for four weeks and they will be transferred to a growth chamber with controlled environment for further establishment. DOI: http://dx.doi.org/10.3329/jbau.v11i2.19841 J. Bangladesh Agril. Univ. 11(2): 171-176, 2013

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