Effects of water stress and rewatering on turnover and gene expression of photosystem II reaction center polypeptide D1 in Zea mays

1999 ◽  
Vol 26 (4) ◽  
pp. 375 ◽  
Author(s):  
Limin Hao ◽  
Houguo Liang ◽  
Zongling Wang ◽  
Xinmin Liu
Keyword(s):  
Water Stress ◽  
Electron Transport ◽  
Photosystem Ii ◽  
Reaction Center ◽  
Control Level ◽  
State Level ◽  
Transcript Level ◽  
Dot Blot ◽  
Psii Reaction Center ◽  
Severe Water Stress

Photosystem II oxygen evolution capacity, the steady-state level of photosystem II (PSII) reaction center polypeptide D1 and its transcript and template levels inZea mays L. (Xinyu No. 4) under water stress and rewatering were studied. The results indicated that PSII and whole-chain electron transport capacities decreased slightly under moderate water stress and appreciably under severe water stress, and could not recover to control level upon rewatering. The results of western and northern blots showed that the content of PSII reaction center polypeptide D1 changed as a similar pattern to PSII and whole-chain electron transport capacities. Dot blot analysis for DNA showed that there was no obvious response of the template level of D1 to water stress or rewatering. From the results, it was concluded that PSII was the major site affected by water stress, where the functional loss of PSII could be attributed to the reduction of PSII reaction center polypeptide D1, which may be caused by the decrease in its transcript level. Rewatering could only ameliorate slightly under moderate water stress but could not recover to control level under severe water stress.

scholarly journals Exploring the Link between Photosystem II Assembly and Translation of the Chloroplast psbA mRNA

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 152 ◽  
Author(s):  
Prakitchai Chotewutmontri ◽  
Rosalind Williams-Carrier ◽  
Alice Barkan
Keyword(s):  
Photosystem Ii ◽  
Water Splitting ◽  
Reaction Center ◽  
Ribosome Profiling ◽  
Oxygenic Photosynthesis ◽  
Dual Roles ◽  
Psii Reaction Center ◽  
Core Proteins ◽  
Prosthetic Groups ◽  
Autoregulatory Mechanism

Photosystem II (PSII) in chloroplasts and cyanobacteria contains approximately fifteen core proteins, which organize numerous pigments and prosthetic groups that mediate the light-driven water-splitting activity that drives oxygenic photosynthesis. The PSII reaction center protein D1 is subject to photodamage, whose repair requires degradation of damaged D1 and its replacement with nascent D1. Mechanisms that couple D1 synthesis with PSII assembly and repair are poorly understood. We address this question by using ribosome profiling to analyze the translation of chloroplast mRNAs in maize and Arabidopsis mutants with defects in PSII assembly. We found that OHP1, OHP2, and HCF244, which comprise a recently elucidated complex involved in PSII assembly and repair, are each required for the recruitment of ribosomes to psbA mRNA, which encodes D1. By contrast, HCF136, which acts upstream of the OHP1/OHP2/HCF244 complex during PSII assembly, does not have this effect. The fact that the OHP1/OHP2/HCF244 complex brings D1 into proximity with three proteins with dual roles in PSII assembly and psbA ribosome recruitment suggests that this complex is the hub of a translational autoregulatory mechanism that coordinates D1 synthesis with need for nascent D1 during PSII biogenesis and repair.

Thiazolylidene-Ketonitriles are Efficient Inhibitors of Electron Transport in Reaction Centers from Photosynthetic Bacteria

1991 ◽  
Vol 46 (11-12) ◽  
pp. 1059-1062 ◽  
Author(s):  
Walter Oettmeier ◽  
Silvana Preuße ◽  
Michael Haefs
Keyword(s):  
Electron Transport ◽  
Photosystem Ii ◽  
Reaction Center ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Bacteria ◽  
Rhodobacter Capsulatus ◽  
Electron Flow ◽  
Reaction Centers ◽  
Inhibitory Potency ◽  
Photosynthetic Electron Flow

Thiazolylidene-ketonitriles are efficient inhibitors of photosynthetic electron flow in reaction centers from either Rhodobacter sphaeroides or Rhodobacter capsulatus. Some compounds of this class exhibit a higher inhibitory potency in the bacterial system as compared to photosystem II. Up to now, photosystem II inhibitors were generally less active in photosynthetic bacteria. An azido-thiazolylidene-ketonitrile upon illumination almost exclusively tags the L-subunit in the bacterial reaction center.

Photosystem II Inhibition by Pyran-enamine Derivatives

1993 ◽  
Vol 48 (3-4) ◽  
pp. 163-167
Author(s):  
Koichi Yoneyama ◽  
Yoshihiro Nakajima ◽  
Masaru Ogasawara ◽  
Hitoshi Kuramochi ◽  
Makoto Konnai ◽  
...  
Keyword(s):  
Electron Transport ◽  
Photosystem Ii ◽  
Thylakoid Membranes ◽  
Major Determinant ◽  
Ps Ii ◽  
Structure Activity Relationships ◽  
Structure Activity

Abstract Through the studies on structure-activity relationships of 5-acyl-3-(1-aminoalkylidene)-4-hydroxy-2 H-pyran-2,6(3 H)-dione derivatives in photosystem II (PS II) inhibition, overall lipophilicity of the molecule was found to be a major determinant for the activity. In the substituted N -benzyl derivatives, not only the lipophilicity but also the electronic and steric characters of the substituents greatly affected the activity. Their mode of PS II inhibition seemed to be similar to that of DCMU , whereas pyran-enamine derivatives needed to be highly lipophilic to block the electron transport in thylakoid membranes, which in turn diminished the permeability through biomembranes.

scholarly journals Physiological and Yield Responses of Green-Shelled Beans (Phaseolus vulgaris L.) Grown under Restricted Irrigation

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 562
Author(s):  
Karen Campos ◽  
Andrés R. Schwember ◽  
Daniel Machado ◽  
Mónica Ozores-Hampton ◽  
Pilar M. Gil
Keyword(s):  
Water Stress ◽  
Deficit Irrigation ◽  
Pot Experiment ◽  
Water Restriction ◽  
One Pot ◽  
Dry Land ◽  
Severe Water Stress ◽  
The Face ◽  
Use Efficiency ◽  
Yield Responses

Common bean is an important crop, consumed as green-shelled bean in several countries. In Chile, green-shelled beans are cultivated often as a dry land crop, vulnerable to drought. The objective of this study was to characterize the hydric and productive responses of four green-shelled bean genotypes subjected to deficit irrigation in order to outline production strategies in the face of increasing water scarcity. Two experiments were evaluated: one pot experiment with three irrigation treatments, supplying 100% of the crop evapotranspiration (ETc) (T100), 50% (T50), and 30% (T30); and an open field experiment with two treatments: 100% (I100) and 40% of ETc (I40). Treatments were applied during reproductive stage in determinate cultivars and vegetative stage in indeterminate plants. Severe water restriction (T30 and I40) in both experiments showed a significant decrease in stomatal conductances, as well as biomass and number of grains per pod; I40 treatment also showed a reduction in chlorophyll fluorescence. Water use efficiency (WUE) was higher under water stress in field (I40), but lower on the T30 treatment from the pot experiment. Determinate cultivars showed 22.7% higher of 100-seed weight compared to indeterminate type, and, thus, higher tolerance to drought. Our results indicate that severe water stress is highly harmful in terms of yield, and a moderate controlled deficit irrigation plus the use of determinate genotypes may be a strategy for producing green-shelled bean successfully under a drought scenario.

Interaction of Photosystem II Herbicides with Bicarbonate and Formate in Their Effects on Photosynthetic Electron Flow

1984 ◽  
Vol 39 (5) ◽  
pp. 374-377 ◽  
Author(s):  
J. J. S. van Rensen
Keyword(s):  
Electron Transport ◽  
Photosystem Ii ◽  
Electron Flow ◽  
Photosynthetic Electron Transport ◽  
Hill Reaction ◽  
Amaranthus Hybridus ◽  
Apparent Affinity ◽  
Photosynthetic Electron Flow ◽  
Different Characteristics ◽  
The Hill

The reactivation of the Hill reaction in CO2-depleted broken chloroplasts by various concentrations of bicarbonate was measured in the absence and in the presence of photosystem II herbicides. It appears that these herbicides decrease the apparent affinity of the thylakoid membrane for bicarbonate. Different characteristics of bicarbonate binding were observed in chloroplasts of triazine-resistant Amaranthus hybridus compared to the triazine-sensitive biotype. It is concluded that photosystem II herbicides, bicarbonate and formate interact with each other in their binding to the Qв-protein and their interference with photosynthetic electron transport.

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