An Open-Circuit Infrared Gas Analysis System for Measuring Aquatic Plant Photosynthesis at Physiological pH

1979 ◽  
Vol 6 (4) ◽  
pp. 493 ◽  
Author(s):  
JA Browse

A temperature-controlled assimilation chamber and gas circuit are described which allow steady-state measurement of photosynthesis by aquatic plants in media containing bicarbonate ions. Gas entering the assimilation chamber is divided into fine bubbles by passage through a sintered glass disc which forms the chamber floor. Rapid achievement and subsequent maintenance of chemical and phase equilibria are ensured by sustaining a high gas flow through the chamber via a recirculating loop in the gas circuit. Incorporation of this unit in a conventional open infrared gas analysis system allowed accurate and sensitive measurements to be made over wide ranges of pH and CO2 concentration.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 576c-576
Author(s):  
E.D. Leonardos ◽  
M.J. Tsujita ◽  
B. Grodzinski ◽  
T.J. Blom

Gas exchange (net photosynthesis Pn, dark respiration, transpiration, and stomatal resistance) of `Jaqueline' Alstroemeria, grown in pots in a greenhouse, were measured. Measurements were made under laboratory conditions using an open-flow infrared gas analysis system for leaf studies, and a semi-closed computer controlled whole plant photosynthesis system for whole plant studies. Apical fully expanded leaves on non-flowering and flowering (at two stages) shoots had similar photosynthetic responses in respect to photosynthetically active radiation (PAR) and to CO2 concentration. Light saturation occurred at 600 umol/m2/s PAR with maximum leaf Pn rates ranging from 9 to 11 umol CO2/m2/s. CO2 saturation was estimated at approximately 1100 to 1200 ppm with maximum leaf Pn rates from 17 to 22 umol CO2/m2/s. Whole plant Pn rates increased with increased PAR. Maximum rates 4 to 5 umol CO2/m2/s (half that of individual leaves) occurred at approximately 1000 to 1100 umol/m2/s PAR. CO2 saturation was estimated at 1100 to 1200 ppm, with maximum whole plant Pn rates ranging from 7 to 8 umol CO2/m2/s. These data will be discussed in relation to respiration and mutual shading at the leaf canopy.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 540a-540
Author(s):  
K.J. Prevete ◽  
R.T. Fernandez

Three species of herbaceous perennials were tested on their ability to withstand and recover from drought stress periods of 2, 4, and 6 days. Eupatorium rugosum and Boltonia asteroides `Snowbank' were chosen because of their reported drought intolerance, while Rudbeckia triloba was chosen based on its reported drought tolerance. Drought stress began on 19 Sept. 1997. Plants were transplanted into the field the day following the end of each stress period. The effects of drought on transpiration rate, stomatal conductance, and net photosynthetic rate were measured during the stress and throughout recovery using an infrared gas analysis system. Leaf gas exchange measurements were taken through recovery until there were no differences between the stressed plants and the control plants. Transpiration, stomatal conductance, and photosynthesis of Rudbeckia and Boltonia were not affected until 4 days after the start of stress. Transpiration of Eupatorium decreased after 3 days of stress. After rewatering, leaf gas exchange of Boltonia and Rudbeckia returned to non-stressed levels quicker than Eupatorium. Growth measurements were taken every other day during stress, and then weekly following transplanting. Measurements were taken until a killing frost that occurred on 3 Nov. There were no differences in the growth between the stressed and non-stressed plants in any of the species. Plants will be monitored throughout the winter, spring, and summer to determine the effects of drought on overwintering capability and regrowth.


1994 ◽  
Vol 40 (1) ◽  
pp. 124-129 ◽  
Author(s):  
R J Wong ◽  
J J Mahoney ◽  
J A Harvey ◽  
A L Van Kessel

Abstract We evaluated a new portable instrument, the PPG StatPal II pH and Blood Gas Analysis System, designed for "point-of-care" measurements of blood gases and pH. Inaccuracy (% of target value) and imprecision (CV%) were assessed by blood tonometry and comparison with a Corning 178. Within-day results for PCO2 inaccuracy and imprecision ranged from 98.2% to 102.9% and 3.3% to 3.9%, respectively; for PO2, these were 95.5% to 102.3% and 2.3% to 3.0%, respectively. Between-day results for PCO2 inaccuracy and imprecision ranged from 99.2% to 99.3% and from 2.9% to 3.2%, respectively; for PO2, the ranges were 96.2% to 98.2% and 2.6% to 3.0%, respectively. Two PCO2 outliers (in 645 samples = 0.3%) were observed. In general, tonometry recovery, measurement stability, and pH bias results for the StatPal II and Corning 178 were comparable. We conclude that the StatPal II performs within acceptable ranges of inaccuracy and imprecision.


2011 ◽  
Vol 9 (6) ◽  
pp. 060101-60104 ◽  
Author(s):  
孙友文 Youwen Sun ◽  
刘文清 Wenqing Liu ◽  
汪世美 Shimei Wang ◽  
黄书华 Shuhua Huang ◽  
于晓曼 Xiaoman Yu
Keyword(s):  

2019 ◽  
Vol 8 (4) ◽  
pp. 3054-3058

The rise of atmospheric carbon dioxide (CO2 )concentration as well as depletion of fossil fuel reserves calls for the development of clean and ecofriendly alternative fuel source. Recently, lipid rich microalgal biomass is being extensively studied for generation of biodiesel however, the expensesincurred on production of microalgal biomassis a significant hurdle. Almost 80 % of the production costis generated from the cultivation medium which majorly comprise of carbon, nitrogen and phosphate. If the microalgal cultivation could be linked to a CO2 capturing unit than the cost of production could be reduced to a large extent. CO2 absorption by means of aqueous amine solvents is known to be a mature technology and could be integrated with microalgal cultivation unit for efficient utilization of the captured CO2 . In this present research work, blended solution of piperazine (PZ) and2-amino2-methyl-1-propanol (AMP) (5/25 wt. %) was used to capture CO2 and then the captured CO2 was utilized as an inorganic carbon stream for growing Chlorella sorokiniana BTA 9031 for biodiesel production. The CO2rate absorption was governed by series of process variablesviz.solvent flow rate ranges (1.5 to 3) ×10⁻4 m 3 min-1 , absorption temperature (298 to 313) K,concentration of CO2 (10 to 15) kPa and gas flow rate(5 to 8) ×10⁻3 m 3 min-1 . The detected final biomass strengthofChlorella sorokiniana BTA 9031 was0.955g L-1 . The fatty acid methyl esters (FAME) determinedsubsequentlyacid transesterification was observed to contain fatty acids suitable for biodiesel production.


1971 ◽  
Vol 24 (4) ◽  
pp. 1077 ◽  
Author(s):  
MM Ludlow ◽  
GL Wilson

Grass and legume plants were grown under near-optimum conditions in controlled-environment cabinets. Changes in net photosynthetic rate, dark respiration rate, and carbon dioxide transfer resistances during leaf ontogeny, and variability between leaves on grass tillers and legume runners were studied under controlled conditions in an open gas analysis system.


2016 ◽  
Vol 11 (2) ◽  
pp. 239
Author(s):  
Arif Dwi Santoso

BPPT conducted the mass of CO2 gas calculation in the gas absorption experiments with phytoplankton cultivation in the photobioreaktor (FBR) batch and continous syatem using the ideal gas equation. This study stated that the method of calculation with the ideal gas equation is more simple and practical in providing data analysis compared with biomass methods. Some things to note in this method include good knowledge about the movement of the gas flow diagram of inputs and outputs FBR, an appropriate gas sampling, and accuracy of measuring instruments. The required data in the mass calculation of CO2 gas in a batch photobioreactor system was resultant CO2 concentration during measurement. Meanwhile in a continuous systems, the requireddata was CO2 concentration at the reactor input and output , the rate and duration of the injection gas.Keywods : massa gas CO2, dry weight, ideal gas formula


2021 ◽  
Vol 8 (12) ◽  
pp. 164
Author(s):  
Eleftherios Karatzanos ◽  
Panagiotis Ferentinos ◽  
Georgios Mitsiou ◽  
Stavros Dimopoulos ◽  
Argyrios Ntalianis ◽  
...  

The purpose of this study was to compare the acute cardiorespiratory responses and time spent above different %VO2peak intensities between three “iso-work” protocols: (a) a high intensity interval training protocol (HIIT), (b) a higher intensity continuous protocol (CON70) and (c) a lower intensity continuous protocol (CON50) in patients with chronic heart failure (CHF). Ten male CHF patients (aged 55.1 ± 16.2 years) performed in separate days a single session of a HIIT protocol consisted of 4 sets × 4 min cycling at 80% VO2peak with 3 min of recovery at 50% VO2peak, a CON70 protocol corresponding to 70% VO2peak and a CON50 protocol corresponding to 50% VO2peak. Cardiopulmonary data were collected by an online gas analysis system. The HIIT and CON70 elicited higher cardiorespiratory responses compared to CON50 with no differences between them (p > 0.05). In HIIT and CON70, patients exercised longer at >80% and >90% VO2peak. The completion rate was 100% for the three protocols. Not any adverse events were observed in either protocol. Both HIIT and CON70 elicited a stronger physiological stimulus and required shorter time than CON50. Both HIIT and CON70 also induced comparable hemodynamic responses and ventilatory demand.


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