Cotyledonary Storage Proteins in Pisum sativum. III. Patterns of Accumulation During Development

1978 ◽  
Vol 5 (4) ◽  
pp. 519 ◽  
Author(s):  
A Millerd ◽  
JA Thomson ◽  
HE Schroeder
Keyword(s):  
Antigenic Determinant ◽  
Storage Proteins ◽  
Subsequent Development ◽  
The Other ◽  
Polypeptide Composition ◽  
Crossed Immunoelectrophoresis ◽  
Antigen Present ◽  
Pea Seeds ◽  
Quantitative Changes ◽  
Change In Mean

An antiserum raised against proteins from isolated protein bodies of mature pea seeds recognized seven antigenically distinct protein families in cotyledon extracts analysed by crossed immunoelectrophoresis. This antiserum has been used to follow the sequence of appearance and pattern of accumulation of these proteins in peas grown under controlled environmental conditions. One antigen present in mature cotyledons was shown to be a haemagglutinin, one corresponded to legumin, and four distinct, non-crossreacting antigenic species were related to the vicilin series of proteins. Both the number of components detected and the order of their appearance during development were similar in three genotypes examined, but the quantitative representation of legumin relative to other components was genotype-specific. Quantitative differences were observed in the polypeptide composition of the vicilin components responsible for separate antigenic specificities, but qualitative differences were not excluded. During development, similar conspicuous quantitative changes in polypeptide composition, accompanied by a change in mean electrophoretic mobility, occurred amongst the molecules carrying the predominant antigenic determinant related to vicilin (peak 4); again, qualitative differences might also distinguish these components. The antigen responsible for peak 6 contained two major polypeptides distinct from the components of the legumin and vicilin fractions. Peak 6 represented the first fully immunocompetent species to appear during development, but later became quantitatively less significant as the other antigenic species accumuIated. Legumin and the antigen forming peak 6 were first detected immunologically in an apparently incomplete form. These putative precursors disappeared during subsequent development, so that only the corresponding stable precipitin peaks were seen in the immunoelectrophoretic profiles of extracts from mature cotyledons.

Cotyledonary Storage Proteins in Pisum sativum. I. Molecular Heterogeneity

1978 ◽  
Vol 5 (3) ◽  
pp. 263 ◽  
Author(s):  
JA Thomson ◽  
HE Schroeder ◽  
WF Dudman
Keyword(s):  
Cellulose Acetate ◽  
Electrophoretic Mobility ◽  
Storage Protein ◽  
Storage Proteins ◽  
Electrophoretic Analysis ◽  
Molecular Heterogeneity ◽  
Polypeptide Composition ◽  
Ph Values ◽  
Pea Seeds ◽  
Differential Solubility

The two storage-protein fractions of pea seeds, legumin and vicilin, have each been resolved electrophoretically on a cellulose acetate gel matrix into multiple molecular species distinguished by electrophoretic mobility and by quantitative or qualitative differences in subunit composition or both. Electrophoretograms of these apparent holoproteins from a range of lines and cultivars were found to be genotype-specific, generally showing three strong bands, together with up to three additional minor bands, assignable to a vicilin series of components. A further three or four bands could be assigned to a legumin series, although the slowest of these showed apparent admixture of certain polypeptides typical of the vicilin fractions. Each putative holoprotein band in both the legumin and vicilin series behaved additively in the electrophoretograms of F1 offspring from reciprocal crosses between lines showing distinct patterns. For comparison with the proteins distinguished electrophoretically on cellulose acetate gels, storage proteins from lyophilized protein bodies were fractionated on the basis of differential solubility at various ionic strengths and pH values. The single legumin and three vicilin fractions obtained by this method showed sedimentation velocities typical of the respective holoproteins. No overlap in the polypeptide composition of legumin with that of vicilin fractions was observed. The components represented in the four fractions accounted for all the major polypeptides in total storage-protein extracts and in the bands eluted from cellulose acetate gels. The distinctive polypeptide pattern and electrophoretic mobility of vicilin fraction 4 identified this protein as a contaminant of slow legumin bands in cellulose acetate gels, and as an additional vicilin species not recognized directly from the electrophoretic analysis.

THE “REVOLUTION” IN FINANCIAL REPORTING THEORY: A KUHNIAN INTERPRETATION

1993 ◽  
Vol 20 (1) ◽  
pp. 33-57 ◽  
Author(s):  
Tom Mouck
Keyword(s):  
Financial Reporting ◽  
Subsequent Development ◽  
The Other ◽  
Other Hand ◽  
Research Problems ◽  
Market Economics ◽  
Economic Income ◽  
The Revolution

A Kuhnian perspective is used to explain the transition in financial reporting theory from an “economic income perspective” to an “informational perspective” (a transition that Beaver refers to as a “revolution”), and to examine the subsequent development of the latter. The demise of the economic income perspective (represented by the normative a priorists) is attributed to the lack of a paradigm which could serve to identify research problems and provide methodological guidance. The success of the informational paradigm, on the other hand, is attributed to the fact that it was, in essence, a sub-paradigm of the broader and well-established market economics paradigm. The study concludes, however, with a discussion of two types of persistent anomalous findings (the first with respect to the EMH and the second with respect to the CAPM) that have the potential to generate a crisis for the informational paradigm.

Changes in Seryl-tRNA Formative in Developing Chick Muscle

1974 ◽  
Vol 52 (10) ◽  
pp. 838-844 ◽  
Author(s):  
Mark Nwagwu ◽  
John Lianga
Keyword(s):  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Deae Cellulose ◽  
The Other ◽  
Embryonic Chick ◽  
Qualitative And Quantitative ◽  
Embryonic Muscle ◽  
Quantitative Changes ◽  
The One

As a prelude to an analysis of the dependence of muscle protein synthesis on aminoacyl tRNA's, we have investigated the rates of seryl-tRNA formation, in vitro, by aminoacylating systems isolated from 11-, 14-, and 17-day chick embryonic muscle. The results show that the combination of 14-day tRNA and 14-day aminoacyl synthetase is the most efficient in seryl-tRNA formation. We have also studied the qualitative and quantitative changes in seryl-tRNA prepared from 11-, 14-, and 17-day embryonic chick muscle by chromatography of seryl-tRNA on benzoylated DEAE-cellulose columns. The results show that, although there are no qualitative differences in the chromatographic patterns of seryl-tRNA from the different ages, there are significant quantitative differences between the patterns for 11-day and 17-day seryl-tRNA on the one hand, and the pattern for 14-day seryl-tRNA on the other.

Basaglia in France

2020 ◽  
pp. 275-294
Author(s):  
Nicolas Henckes ◽  
Anne M. Lovell
Keyword(s):  
Subsequent Development ◽  
Second World War ◽  
The Other ◽  
World War ◽  
Community Based ◽  
Institutional Practice ◽  
The One ◽  
Mental Health Policies ◽  
Second World ◽  
French Paradox

This chapter assesses Franco Basaglia’s enduring influence in France by focusing on the circulation of concepts and practices and their effects on French mental health policies and scattered experimentation. Despite similar origins, Basaglia’s early work contrasts with the Second World War movement of French psychiatric reformers to humanize the asylum, including through ‘psychothérapie institutionnelle’ and the subsequent development of a sectorization policy. The chapter then examines the extent to which Basaglia’s ideas took ground in France through the efforts of a small network of psychiatric practitioners and intellectuals, within roughly three periods: 1960–1980, 1980–2000, and 2000 to the present. In conclusion, the chapter asks what might explain the French paradox: the early receptivity to Basaglia’s politically-oriented, community-based, anti-institutional practice, on the one hand; and a tenacious hospital-centric psychiatric system and increased use of constraints and high-security confinement, on the other.

Age Differences in Coping: Does Less Mean Worse?

1989 ◽  
Vol 28 (2) ◽  
pp. 127-140 ◽  
Author(s):  
Suzanne Meeks ◽  
Laura L. Carstensen ◽  
Brenda-Fay Tamsky ◽  
Thomas L. Wright ◽  
David Pellegrini
Keyword(s):  
Older Adults ◽  
Older People ◽  
Coping Strategies ◽  
Age Differences ◽  
Adaptive Strategies ◽  
The Other ◽  
The Third ◽  
Older Subjects ◽  
Quantitative Changes

Previous research suggests that elderly people utilize fewer coping strategies than younger people. Some researchers suggest that these quantitative changes reflect decreases in the use of maladaptive strategies; others contend that they reflect decreases in the use of adaptive strategies by older adults. The present article reports the findings of three studies of coping in older people, two addressing coping with health problems, and the other addressing coping with moving. In all three studies, the number of self-reported coping strategies decreases with age. Results do not support the idea that decreases in the number of strategies imply decrements in the quality of coping, however: in two studies, age was unrelated to the effectiveness of strategies, in the third, effectiveness ratings were higher for older subjects. The need for evaluation of specific outcomes of coping strategies is discussed, along with the need for task-specific measurement of coping. It is proposed that decreases in the number of coping strategies reflect improved coping efficiency, rather than a deterioration of adaptational skills.

scholarly journals CARRIAGE OF THE VIRUS OF POLIOMYELITIS, WITH SUBSEQUENT DEVELOPMENT OF THE INFECTION

1917 ◽  
Vol 26 (5) ◽  
pp. 745-754 ◽  
Author(s):  
Edward Taylor ◽  
Harold L. Amoss
Keyword(s):  
Subsequent Development ◽  
Immune Serum ◽  
Family Group ◽  
The Other ◽  
Source Of Infection ◽  
Initial Symptoms ◽  
Original Case ◽  
Perfect Recovery

A family group containing four children of whom all showed in varying degree symptoms of poliomyelitis is described. The source of infection and periods of incubation have been followed. Two of the children were proven by inoculation tests to carry the virus of poliomyelitis in the nasopharynx. Of these, one was detected to be a carrier after recovering from a non-paralytic attack of the disease, and the other was discovered to be a carrier about 5 days before the initial symptoms, attended later by paralysis, appeared. The original case from which the three others took origin was fatal; the youngest child, after quite a severe onset, was treated with immune serum, and made a prompt and almost perfect recovery. The nasopharyngeal secretions of two of the cases, taken 1 month after the attack, proved incapable of neutralizing an active poliomyelitic virus. The proposition is presented that every case of poliomyelitis develops from a carrier of the microbic cause, or virus, of poliomyelitis.

Cotyledonary Storage Proteins in Pisum Sativum. II. Hereditary Variation in Components of the Legumin and Vicilin Fractions

1978 ◽  
Vol 5 (3) ◽  
pp. 281 ◽  
Author(s):  
JA Thomson ◽  
HE Schroeder
Keyword(s):  
Pisum Sativum ◽  
Phenotypic Variation ◽  
Storage Protein ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Gene Products ◽  
Hereditary Variation ◽  
Pea Seeds ◽  
Parental Lines ◽  
Variant System

Gel electrophoresis has been used to investigate genetically controlled variation in storage-protein constituents forming five series of bands (LA-LE) derived from legumin fractions, and three series of bands (VA-VC) from vicilin fractions, of pea seeds. In each variant system, the phenotypes of the storage-protein polypeptides from F1 seeds were additive with respect to the band patterns of the parental lines, and identical in reciprocal crosses. Neither dominance nor formation of new interaction products was observed. Variation in the three systems involving vicilin polypeptides and two of those involving legumin components was found to be based on allelic alternatives at single loci designated Vicilin A (Vca), Vicilin B (Vcb), Vicilin C (Vcc), Legumin A (Lga) and Legumin C (Lgc). For each of these variant systems, the gene products involved and the basis of the phenotypic variation have been discussed. Variants of the VC band complex, in which mobility of two bands both composed of 12 and 14 kdalton polypeptides is altered, appear likely to correspond to vicilin variants described previously. Type lines are specified for each of the variant phenotypes analysed, and for the genes designated.

Quantitative changes of organelles in early cirrhosis of rat

1988 ◽  
Vol 46 ◽  
pp. 332-333
Author(s):  
J. W. Ryoo ◽  
R. J. Buschmann
Keyword(s):  
Electron Microscopy ◽  
Left Lobe ◽  
The Other ◽  
Cell Cytoplasm ◽  
Ito Cell ◽  
Functional Changes ◽  
Regenerative Nodules ◽  
Quantitative Changes ◽  
Rat Livers ◽  
Subcellular Level

Cirrhosis involves major architectural changes in the liver. The changes are appreciated histologically as regenerative nodules and septa, but they do not reflect directly the structural/functional changes which must occur at the subcellular level. In order to test for these subcellular changes, we chose to carry out a morphometric analysis of two organelle systems that are most likely to be affected in liver cirrhosis.Early cirrhosis was produced by CC14 inhalation along with phenobarbital continuously in the drinking water (CCl4+PB). Two groups of control rats were used. One group was given neither the CCI4 nor the phenobarbital (CONT); the other group was given just the phenobarbital (PB). After 8 wk the rat livers were fixed by perfusion, The left lobe was isolated, weighed, and its volume determined. Tissue was prepared for electron microscopy. Micrographs of random areas of hepatocyte cytoplasm (36000x), hepatocyte mitochondria (90000x), and Ito cell cytoplasm (54000x).

scholarly journals Major loss of the 28-kD protein of gap junction in proliferating hepatocytes.

1987 ◽  
Vol 105 (4) ◽  
pp. 1925-1934 ◽  
Author(s):  
R Dermietzel ◽  
S B Yancey ◽  
O Traub ◽  
K Willecke ◽  
J P Revel
Keyword(s):  
Monoclonal Antibody ◽  
Gap Junction ◽  
The Other ◽  
Regenerating Liver ◽  
Cultured Hepatocytes ◽  
Junction Protein ◽  
Major Loss ◽  
Gap Junction Protein ◽  
Quantitative Changes ◽  
The Relationship

There is a reduction in the 28-kD gap junction protein detectable by immunofluorescence in livers of partially hepatectomized rats and in cultured hepatocytes stimulated to proliferate. By the coordinate use of antibodies directed to the hepatic junction protein (HJP28) and the use of a monoclonal antibody that recognizes bromodeoxyuridine (BrdU) incorporated into DNA, we have been able to study the relationship between detectable gap junction protein and cell division. Hepatocytes that label with BrdU in the regenerating liver and in cell culture show a significant reduction of HJP28. Cells that do not synthesize DNA, on the other hand, show normal levels and distribution of immunoreactive gap junction protein. We postulate that the quantitative changes in gap junction expression might play an important role in the control of proliferation in the liver.

PIXE STUDIES ON POTASSIUM AND CALCIUM IN MOUSE BLOOD PLASMA AFTER TRANSPLANTATION OF EL-4 TUMOR CELLS

1995 ◽  
Vol 05 (04) ◽  
pp. 255-264 ◽  
Author(s):  
ITSURO TAMANOI ◽  
AKEMI NAKAMURA ◽  
KIYOFUSA HOSHIKAWA ◽  
MUTSUMI KACHI ◽  
KUNIO OOHASHI ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Tumor Cells ◽  
Time Course ◽  
The Other ◽  
Histocompatibility Antigens ◽  
Mouse Blood ◽  
Major Histocompatibility Antigens ◽  
The Difference ◽  
Quantitative Changes ◽  
Pixe Method

The quantitative changes in the elements, amounts of Cl, K, Ca, in blood plasma were measured by PIXE method. The samples were obtained at appropriate intervals after transplantation of EL-4 tumor cells in three strains of mice, C57BL/6J (H-2b), C57BL/10J (abbreviation: B10; H-2b) and A/J (H-2a). Transplanted EL-4 tumor cells proliferated in both strains of C57BL/6J and B10. In A/J mice, transplanted EL-4 cells proliferated about 10 days and then were rejected completely by the immunological reaction according to the difference of major histocompatibility antigens. The amounts of Cl in plasma remained at similar level in the time course in any strains, but K fluctuated in C57BL/6J and B10, and less in A/J. On the other hand, Ca showed always higher values in C57BL/6J than other two strains of mice. In B10 mice, Ca increased just before death, but in A/J it decreased at the time of healing by rejection. These changes of Ca in the three strains of mice were related quantitatively 10 the hematocrit values of these strains of mice after transplantation of EL-4 cells.

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