Two CO2 uptake systems in cyanobacteria: four systems for inorganic carbon acquisition in Synechocystis sp. strain PCC6803

2002 ◽  
Vol 29 (3) ◽  
pp. 123 ◽  
Author(s):  
Mari Shibata ◽  
Hiroshi Ohkawa ◽  
Hirokazu Katoh ◽  
Masaya Shimoyama ◽  
Teruo Ogawa
Keyword(s):  
Promoter Region ◽  
Inorganic Carbon ◽  
Co2 Uptake ◽  
Coding Region ◽  
Synechocystis Pcc6803 ◽  
Low Co2 ◽  
Neutral Site ◽  
Sodium Dependent ◽  
Synechocystis Sp ◽  
Inorganic Carbon Acquisition

The cyanobacterium Synechocystis sp. strain PCC6803 possesses two CO2 uptake systems; one constitutive, dependent on NdhD3/NdhF3/CupA (Sll1734), and one low-CO2 inducible, dependent on NdhD4/NdhF4/CupB (Slr1302). Homologues of these genes are present in pairs in most cyanobacterial strains. Synechocystis PCC6803 also possesses two types of HCO3– transporters; an ATP-binding cassette (ABC)-type transporter encoded by the cmp operon, and a novel sodium-dependent transporter encoded byslr1512(sbtA) that plays a central role in HCO3– uptake. Mutants impaired for one of these four inorganic-carbon acquisition systems did not show mutant phenotype. Mutants inactivated for both CO2 uptake systems were unable to grow at pH 7.0 in air, although they grew normally at pH 9.0 in air. Additional inactivation of the SbtA-type HCO3– transporter abolished growth at pH 9.0 in air. A fragment containing the promoter region of ndhF3 fused to the coding region of luxAB was inserted into a neutral site of the ΔndhD4 mutant to construct apF3-lux/ ΔndhD4 strain. The luminescence intensity of this strain was low in high-CO2 grown cells, and was increased about 100 times after acclimation to air. Inactivation of the pF3-lux/ ΔndhD4 strain with a transposon-tagging library enabled us to isolate mutants incapable of acclimation to low CO2.

FtsH protease is required for induction of inorganic carbon acquisition complexes in Synechocystis sp. PCC 6803

2007 ◽  
Vol 65 (3) ◽  
pp. 728-740 ◽  
Author(s):  
Pengpeng Zhang ◽  
Cosmin I. Sicora ◽  
Natalia Vorontsova ◽  
Yagut Allahverdiyeva ◽  
Natalia Battchikova ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Ftsh Protease ◽  
Synechocystis Sp ◽  
Pcc 6803 ◽  
Inorganic Carbon Acquisition

The diversity of inorganic carbon acquisition mechanisms in eukaryotic microalgae

2002 ◽  
Vol 29 (3) ◽  
pp. 261 ◽  
Author(s):  
Brian Colman ◽  
I. Emma Huertas ◽  
Shabana Bhatti ◽  
Jeffrey S. Dason
Keyword(s):  
Active Transport ◽  
Active Site ◽  
Inorganic Carbon ◽  
Algal Species ◽  
Isochrysis Galbana ◽  
Co2 Uptake ◽  
Ecological Distribution ◽  
Aquatic Medium ◽  
Low Co2 ◽  
Marine Dinoflagellates

Eukaryotic microalgae have developed CO2concentrating mechanisms to maximise the concentration of CO2 at the active site of Rubisco in response to the low CO2 concentrations in the external aquatic medium. In these organisms, the modes of inorganic carbon (Ci) uptake are diverse, ranging from diffusive CO2 uptake to the active transport of HCO3 -and CO2 and many have an external carbonic anhydrase to facilitate HCO3- use. There is unequivocal evidence for the mechanisms of Ci uptake in only about 25 species of microalgae of the chlorophyte, haptophyte, rhodophyte, diatom, and eustigmatophyte groups. Most of these species take up both CO2 and HCO3-, but the rates of uptake of each of these substrates varies with the algal species. A few species take up only one of the two forms of Ci, an adaptation that is not necessarily correlated with their ecological distribution. Evidence is presented for the active uptake of HCO3- and CO2 in two marine haptophytes,Isochrysis galbana Parke and Dicrateria inornata Parke, and for active transport of CO2 but lack of HCO3- uptake in two marine dinoflagellates, Amphidinium carteraeHulburt and Heterocapsa oceanica Stein.

Expression of Inducible Inorganic Carbon Acquisition Complexes Is Under the Control of the FtsH Protease in Synechocystis sp. PCC 6803

2008 ◽  
pp. 829-833
Author(s):  
Pengpeng Zhang ◽  
Cosmin I. Sicora ◽  
Natalia Vorontsova ◽  
Yagut Allahverdiyeva ◽  
Natalia Battchikova ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Ftsh Protease ◽  
Synechocystis Sp ◽  
Pcc 6803 ◽  
Inorganic Carbon Acquisition

scholarly journals The Entner-Doudoroff Pathway Contributes to Glycogen Breakdown During High to Low CO2 Shifts in the Cyanobacterium Synechocystis sp. PCC 6803

2021 ◽  
Vol 12 ◽  
Author(s):  
Stefan Lucius ◽  
Alexander Makowka ◽  
Klaudia Michl ◽  
Kirstin Gutekunst ◽  
Martin Hagemann
Keyword(s):  
Organic Compounds ◽  
Inorganic Carbon ◽  
Pentose Phosphate ◽  
Oxygenic Photosynthesis ◽  
Wild Type ◽  
Low Co2 ◽  
Glycogen Breakdown ◽  
Synechocystis Sp ◽  
Mutant Collection ◽  
Pcc 6803

Cyanobacteria perform plant-like oxygenic photosynthesis to convert inorganic carbon into organic compounds and can also use internal carbohydrate reserves under specific conditions. A mutant collection with defects in different routes for sugar catabolism was studied to analyze which of them is preferentially used to degrade glycogen reserves in light-exposed cells of Synechocystis sp. PCC 6803 shifted from high to low CO2 conditions. Mutants defective in the glycolytic Embden–Meyerhof–Parnas pathway or in the oxidative pentose-phosphate (OPP) pathway showed glycogen levels similar to wild type under high CO2 (HC) conditions and were able to degrade it similarly after shifts to low CO2 (LC) conditions. In contrast, the mutant Δeda, which is defective in the glycolytic Entner-Doudoroff (ED) pathway, accumulated elevated glycogen levels under HC that were more slowly consumed during the LC shift. In consequence, the mutant Δeda showed a lowered ability to respond to the inorganic carbon shifts, displayed a pronounced lack in the reactivation of growth when brought back to HC, and differed significantly in its metabolite composition. Particularly, Δeda accumulated enhanced levels of proline, which is a well-known metabolite to maintain redox balances via NADPH levels in many organisms under stress conditions. We suggest that deletion of eda might promote the utilization of the OPP shunt that dramatically enhance NADPH levels. Collectively, the results point at a major regulatory contribution of the ED pathway for the mobilization of glycogen reserves during rapid acclimation to fluctuating CO2 conditions.

Photosynthesis and inorganic carbon acquisition in the cyanobacterium Chlorogloeopsis sp. ATCC 27193

1997 ◽  
Vol 99 (1) ◽  
pp. 81-88
Author(s):  
Robert S. Skleryk ◽  
Pascal N. Tyrrell ◽  
George S. Espie
Keyword(s):  
Inorganic Carbon ◽  
Inorganic Carbon Acquisition

Sequence variants in the FcεRI alpha chain gene

2002 ◽  
Vol 93 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Toshiki Shikanai ◽  
Eric S. Silverman ◽  
Brian W. Morse ◽  
Craig M. Lilly ◽  
Hiroshi Inoue ◽  
...  
Keyword(s):  
Promoter Region ◽  
Population Substructure ◽  
Chain Gene ◽  
Polymorphism Analysis ◽  
Sequence Variants ◽  
Nucleotide Polymorphisms ◽  
Coding Region ◽  
Alpha Chain ◽  
Asthmatic Patients ◽  
Single Stranded Conformational Polymorphism

There is a relationship between IgE levels and expression of high-affinity IgE receptors (FcεRI). Because the alpha chain is the only portion of the receptor that binds directly to IgE, we reasoned that sequence variants in the FcεRI alpha gene may exist that alter these binding events. We screened all of the exons and the promoter region of the FcεRI alpha chain gene with genomic DNA from 389 asthmatic and 341 normal control subjects for mutations by using single-stranded conformational polymorphism analysis. No nonsynonomous single nucleotide polymorphisms (SNPs) were identified in the coding region. Three SNPs were found in the promoter region: an A/C transversion at −770 from the translation start site; a G/A transition at −664; and a T/C transition at −335. No differences in allele frequencies were detected between asthmatic subjects and controls. Homozygosity for the C variant at locus −335 was more common in Caucasian asthmatic patients with IgE levels in the lower quartile than in the upper quartile ( P = 0.032). An analysis of highly polymorphic SNPs indicated that this association is unlikely to be due to population substructure. We conclude that homozygosity for the C allele of FcεRI alpha chain variant is associated with lower IgE levels.

scholarly journals Impact of Inorganic Carbon Availability on Microcystin Production by Microcystis aeruginosa PCC 7806

2007 ◽  
Vol 73 (21) ◽  
pp. 6994-7002 ◽  
Author(s):  
Sabine J�hnichen ◽  
Tilo Ihle ◽  
Thomas Petzoldt ◽  
J�rgen Benndorf
Keyword(s):  
Microcystis Aeruginosa ◽  
Type Strain ◽  
Inorganic Carbon ◽  
Wild Type Strain ◽  
Light Cycle ◽  
Variable Fluorescence ◽  
Wild Type ◽  
Dark Light ◽  
Sodium Dependent ◽  
The Impact

ABSTRACT Batch culture experiments with the cyanobacterium Microcystis aeruginosa PCC 7806 were performed in order to test the hypothesis that microcystins (MCYSTs) are produced in response to a relative deficiency of intracellular inorganic carbon (Ci,i). In the first experiment, MCYST production was studied under increased Ci,i deficiency conditions, achieved by restricting sodium-dependent bicarbonate uptake through replacement of sodium bicarbonate in the medium with its potassium analog. The same experimental approach was used in a second experiment to compare the response of the wild-type strain M. aeruginosa PCC 7806 with its mcyB mutant, which lacks the ability to produce MCYSTs. In a third experiment, the impact of varying the Ci,i status on MCYST production was examined without suppressing the sodium-dependent bicarbonate transporter; instead, a detailed investigation of a dark-light cycle was performed. In all experiments, a relative Ci,i deficiency was indicated by an elevated variable fluorescence signal and led to enhanced phycocyanin cell quotas. Higher MCYST cell quotas (in the first and third experiments) and increased total (intracellular plus extracellular) MCYST production (in the first experiment) were detected with increased Ci,i deficiency. Furthermore, the MCYST-producing wild-type strain and its mcyB mutant showed basically the same response to restrained inorganic carbon uptake, with elevated variable fluorescence and phycocyanin cell quotas with increased Ci,i deficiency. The response of the wild type, however, was distinctly stronger and also included elevated chlorophyll a cell quotas. These differences indicate the limited ability of the mutant to adapt to low-Ci,i conditions. We concluded that MCYSTs may be involved in enhancing the efficiency of the adaptation of the photosynthetic apparatus to fluctuating inorganic carbon conditions in cyanobacterial cells.

scholarly journals Identification of Two Genes, sll0804 and slr1306, as Putative Components of the CO2-Concentrating Mechanism in the Cyanobacterium Synechocystis sp. Strain PCC 6803

2008 ◽  
Vol 190 (24) ◽  
pp. 8234-8237 ◽  
Author(s):  
Shulu Zhang ◽  
Kevin W. Spann ◽  
Laurie K. Frankel ◽  
James V. Moroney ◽  
Terry M. Bricker
Keyword(s):  
Inorganic Carbon ◽  
Carbon Concentrating Mechanism ◽  
Co2 Concentrating Mechanism ◽  
Concentrating Mechanism ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT Insertional transposon mutations in the sll0804 and slr1306 genes were found to lead to a loss of optimal photoautotrophy in the cyanobacterium Synechocystis sp. strain PCC 6803 grown under ambient CO2 concentrations (350 ppm). Mutants containing these insertions (4BA2 and 3ZA12, respectively) could grow photoheterotrophically on glucose or photoautotrophically at elevated CO2 concentrations (50,000 ppm). Both of these mutants exhibited an impaired affinity for inorganic carbon. Consequently, the Sll0804 and Slr1306 proteins appear to be putative components of the carbon-concentrating mechanism in Synechocystis sp. strain PCC 6803.

scholarly journals Optimal Mixture Design of Low-CO2 High-Volume Slag Concrete Considering Climate Change and CO2 Uptake

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Han-Seung Lee ◽  
Seung-Min Lim ◽  
Xiao-Yong Wang
Keyword(s):  
Climate Change ◽  
Design Method ◽  
High Strength ◽  
High Volume ◽  
Mixture Design ◽  
Control Factor ◽  
Co2 Uptake ◽  
Uptake Ratio ◽  
Carbonation Depth ◽  
Low Co2

Abstract High-volume slag (HVS) can reduce the CO2 emissions of concrete, but increase the carbonation depth of concrete. In particular, because of the effects of climate change, carbonation will accelerate. However, the uptake of CO2 as a result of carbonation can mitigate the harm of CO2 emissions. This study proposes an optimal mixture design method of low-CO2 HVS concrete considering climate change, carbonation, and CO2 uptake. Firstly, net CO2 emissions are calculated by subtracting the CO2 emitted by the material from the uptake of CO2 by carbonation. The strength and depth of carbonation are evaluated by a comprehensive model based on hydration. Secondly, a genetic algorithm (GA) is used to find the optimal mixture. The objective function of the GA is net CO2 emissions. The constraints of the GA include the strength, carbonation, workability, and range of concrete components. Thirdly, the results show that carbonation durability is a control factor of the mixture design of low-strength HVS concrete, while strength is a control factor of the mixture design of high-strength HVS concrete. After considering climate change, the threshold of strength control increases. With the increase of strength, the net CO2 emissions increase, while the CO2 uptake ratio decreases.

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