Identification of proteins regulated by cross-talk between drought and hormone pathways in Arabidopsis wild-type and auxin-insensitive mutants, axr1 and axr2

2002 ◽  
Vol 29 (1) ◽  
pp. 55 ◽  
Author(s):  
Michele Wolfe Bianchi ◽  
Catherine Damerval ◽  
Nicole Vartanian
Keyword(s):  
Oxidative Stress ◽  
Abscisic Acid ◽  
Drought Stress ◽  
Cross Talk ◽  
Late Embryogenesis Abundant ◽  
Adaptive Responses ◽  
Wild Type ◽  
Late Embryogenesis ◽  
Differential Behaviour ◽  
Related Proteins

Ten proteins differentially regulated by progressive drought stress in Arabidopsis Columbia wild-type, axr1-3 and axr2-1auxin-insensitive mutants, were identified from internal amino acid microsequencing. These proteins fell into two categories: (i) stress-related proteins, known to be induced by rapid water stress via abscisic acid (ABA)-dependent or -independent pathways [late embryogenesis abundant (LEA)-like and heat shock cognate (HS) 70, respectively], or in response to pathogens or oxidative stress [β-1,3 glucanase (BG), annexin] and (ii) metabolic enzymes [glutamine synthetase (GS), fructokinase (Frk), caffeoyl-CoA-3-O-methyltransferase (CCoAOMT)]. The differential behaviour of these proteins highlighted a role for AXR2 and/or AXR1 in the regulation of their abundance during drought adaptation. In particular, reduced induction of RD29B, GS and annexin, and overexpression of BG2 were observed specifically in the axr1-3 mutant, which is dramatically affected in several ABA-dependent drought adaptive responses, such as drought rhizogenesis. Altogether these results indicate cross-talk between auxin- and ABA-signalling in Arabidopsis drought responses.

Abscisic acid induction of cloned cotton late embryogenesis-abundant (Lea) mRNAs

1986 ◽  
Vol 7 (3) ◽  
pp. 155-170 ◽  
Author(s):  
Glenn A. Galau ◽  
D. Wayne Hughes ◽  
Leon Dure
Keyword(s):  
Abscisic Acid ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis

scholarly journals Drought Tolerance in Wheat

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Arash Nezhadahmadi ◽  
Zakaria Hossain Prodhan ◽  
Golam Faruq
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Review Paper ◽  
Expression Patterns ◽  
Late Embryogenesis Abundant ◽  
Glutathione S Transferase ◽  
Molecular Responses ◽  
Drought Tolerant ◽  
Late Embryogenesis ◽  
Different Types

Drought is one of the most important phenomena which limit crops’ production and yield. Crops demonstrate various morphological, physiological, biochemical, and molecular responses to tackle drought stress. Plants’ vegetative and reproductive stages are intensively influenced by drought stress. Drought tolerance is a complicated trait which is controlled by polygenes and their expressions are influenced by various environmental elements. This means that breeding for this trait is so difficult and new molecular methods such as molecular markers, quantitative trait loci (QTL) mapping strategies, and expression patterns of genes should be applied to produce drought tolerant genotypes. In wheat, there are several genes which are responsible for drought stress tolerance and produce different types of enzymes and proteins for instance, late embryogenesis abundant (lea), responsive to abscisic acid (Rab), rubisco, helicase, proline, glutathione-S-transferase (GST), and carbohydrates during drought stress. This review paper has concentrated on the study of water limitation and its effects on morphological, physiological, biochemical, and molecular responses of wheat with the possible losses caused by drought stress.

scholarly journals Isolasi dan karakterisasi gen dehydrin dari tebu (Saccharum officinarumL.) yang terlibat dalam respon toleransi cekaman kekeringan (Isolation and characterization of dehydrin gene from sugarcane (Saccharum officinarum L.) involved in drought tolerance response)

2018 ◽  
Vol 86 (2) ◽  
Author(s):  
Hayati MINARSIH ◽  
. FANIAR ◽  
Tati KRISTANTI ◽  
Dian M AMANAH ◽  
. SUSTIPRIJATNO ◽  
...  
Keyword(s):  
Drought Stress ◽  
Saccharum Officinarum ◽  
Late Embryogenesis Abundant ◽  
High Homology ◽  
Rt Pcr ◽  
Literature Study ◽  
Coding Sequence ◽  
Isolation And Characterization ◽  
Late Embryogenesis ◽  
Tolerance Response

Nowadays,the development of molecular biology techniques has enabled to engineer drought tolerant sugarcane to accelerate thebreeding program. Dehydrin(DHN)that belong to the group II late embryogenesis abundant (LEA) family is known to havean important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). Literature study and bioinformatics analysis reported that DHN1gene on sugarcane showed high homology sequences with sorghum DHN. The expression of DHN1gene on sugarcane var. PSJT 941 treated with various periodof drought stress had been conducted using semi-quantitative reverse transcriptase (RT)-PCR method. The results showed that the expressionlevel of DHN1 geneincreased along withthe increased period of the treatment. The highest expression level of DHN1 gene was resulted from plants that had been subjected to drought for 25 days. Amplification of DHN1gene  from plants withthe highest gene expression, resulted an amplicon  with a size of 465 bp which representsa full length coding sequence (CDS) of DHN1. Identification using Blast analysis showed that DHN1sequences  from sugarcane var. PSJT 941 shared high homology with DHN gene on sugarcane and sorghum. The alignment results also revealed a conserved motif that characterized DHN genes.[Key words: drought stress, dehydrin, DHN1gene, sugarcane]Abstrak Dengan berkembangnya teknik biologi molekuler saat ini, maka perakitan tanaman tebu yang toleran kekeringan lebih diarahkan melalui teknik rekayasa genetika untuk mempercepat program pemuliaan tanaman.  Protein dehydrin (DHN) yang termasuk ke dalam kelompok II  famili LEA (Late Embryogenesis Abundant)diketahui berperan penting dalam respon dan adaptasi tanaman terhadap cekaman abiotik (kekeringan, salinitas tinggi, suhu dingin, panas, dll). Studi literatur dan analisis bioinformatika menunjukkan bahwa gen DHN1pada tanaman tebu memiliki homologi yang tinggi dengan gen DHNpada sorghum. Analisis ekspresi gen DHN1pada tanaman  tebu varietasPSJT 941yang diberi cekaman kekeringan telah dilakukan menggunakan semi-kuantitatifreverse transcriptase (RT)-PCR dan terlihat bahwa ekspresi gen DHN1meningkat secara nyata sejalan dengan semakin lamanya waktu pemberian cekaman. Tingkat ekspresi gen DHN1paling tinggi diperoleh dari tanaman yang mengalami cekaman kekeringan selama 25 hari.  Amplifikasi gen DHN1pada tanaman dengan tingkat ekspresi yang paling tinggi menunjukkan pita dengan ukuran 465 bp yang merepresentasikan full coding sequence(CDS) gen DHN1. Identifikasi menggunakan analisis Blast menunjukkan bahwa sekuen gen DHN1dari tanaman tebu varietas PSJT 941yang diperoleh memiliki homologi yang tinggi dengan gen DHNpada tanaman tebu dan sorghum. Hasil penjajaran sekuen protein juga menunjukkan adanya motif lestari yang mencirikan gen DHN. [Kata kunci: cekaman kekeringan, dehydrin, gen DHN1, tebu]

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