Towards a molecular taxonomy for the bloom-forming cyanobacteria

1994 ◽  
Vol 45 (5) ◽  
pp. 869 ◽  
Author(s):  
BA Neilan ◽  
PR Hawkins ◽  
PT Cox ◽  
AE Goodman
Keyword(s):  
Dna Sequence ◽  
Ribosomal Rna ◽  
Water Samples ◽  
Molecular Taxonomy ◽  
Sequence Information ◽  
Specific Sequence ◽  
Ribosomal Rna Gene ◽  
Toxigenic Strains

Regions of the 16s subunit of the ribosomal RNA gene of the bloom-associated cyanobacterial genera Microcystis and Anabaena have been sequenced and found to provide strain-specific sequence information. Comparisons of the DNA sequence of these cyanobacteria indicated that the strains examined are related and that probes diagnostic for bloom-forming and toxigenic strains in water samples can be designed.

Hypervariation associated with a 12-nucleotide direct repeat and inferences on intergenomic homogenization of ribosomal RNA gene spacers based on the DNA sequence of a clone from the wheat Nor-D3 locus

Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 770-781 ◽  
Author(s):  
Michael Lassner ◽  
Olin Anderson ◽  
Jan Dvořák
Keyword(s):  
Dna Sequence ◽  
Ribosomal Rna ◽  
Consensus Sequence ◽  
Direct Repeat ◽  
Triticum Aestivum L ◽  
Sequence Information ◽  
D Genome ◽  
Ribosomal Rna Gene ◽  
Nontranscribed Spacer ◽  
B Genome

A ribosomal RNA gene (rDNA) unit from the Nor-D3 locus (D genome) of Triticum aestivum L. was cloned and the "nontranscribed spacer" (NTS) was sequenced. The DNA sequence was compared with previously reported Nor-B2 locus (B genome) NTS sequences to study the molecular basis of evolution of these repeated genes and to look for evidence of homogenization between B- and D-genome rDNA. The NTS has seven subrepeats with a modal repeat length of 120 nucleotides; the subrepeats are shorter than Nor-B2 subrepeats owing to loss of one element of a 12-bp duplication present in Nor-B2 subrepeats. This 12 nucleotide sequence or its permutation, whose consensus sequence is CACGTACACGGA, is found at all sites where the B- and D-genome rDNA spacers differ by insertions or deletions longer than two nucleotides. The DNA sequence information was used to identify restriction sites unique to each locus that could be used in search of conversions between the B- and D-genome rDNA loci. Despite the coexistence of rDNA of the B- and D-genomes in the same nucleus for a minimum of 8000 years, no evidence for frequent interchromosomal conversion events between chromosomes 1B or 6B and 5D was found. Key words: Triticum, rDNA, concerted evolution, spacer.

Genetic Relationship Among Indian Termites Based on DNA Sequence of Mitochondrial 12S Ribosomal RNA Gene

2015 ◽  
Vol 02 (01) ◽  
Author(s):  
Mandakini Singla Vijay Lakshmi ◽  
Sharma Ranbir Chander Sobti ◽  
Monika Sodhi Mamtesh Kumari
Keyword(s):  
Dna Sequence ◽  
Ribosomal Rna ◽  
Genetic Relationship ◽  
Ribosomal Rna Gene

scholarly journals The intergenic spacer region of the ribosomal RNA gene of Penicillium marneffei shows almost no DNA sequence diversity

2010 ◽  
Vol 54 (11) ◽  
pp. 714-716 ◽  
Author(s):  
Nanthawan Mekha ◽  
Takashi Sugita ◽  
Koichi Makimura ◽  
Natteewan Poonwan ◽  
Pathom Sawanpanyalert ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Ribosomal Rna ◽  
Intergenic Spacer ◽  
Sequence Diversity ◽  
Penicillium Marneffei ◽  
Ribosomal Rna Gene ◽  
Intergenic Spacer Region

scholarly journals Chemical Lineages of Ligularia Fischeri

2016 ◽  
Vol 11 (2) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Chiaki Kuroda ◽  
Chiemi Shibayama ◽  
Kyosuke Inoue ◽  
Yasuko Okamoto ◽  
Motoo Tori ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Ribosomal Rna ◽  
Chemical Diversity ◽  
Internal Transcribed Spacers ◽  
Ribosomal Rna Gene ◽  
Ligularia Fischeri ◽  
Root Chemicals

Six Ligularia fischeri samples, two from Sichuan (samples 1 and 2) and four from Chongqing (samples 3–6), were examined for root chemicals and the DNA sequence of the internal transcribed spacers of the ribosomal RNA gene. Samples 2 and 3 contained benzofurans. The isolation of benzofurans shows that the chemical diversity in L. fischeri is higher than previously reported. Samples 1, 4, 5, and 6 contained eremophilanes. However, the compounds were different between sample 1 and samples 4–6, indicating variation within eremophilane producers. DNA data indicated that introgression could be a mechanism of benzofuran production in sample 2 and that sample 1 and samples 4–6 were genetically separate.

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