Longevity in maternal transmission of isotopic marks in a tropical freshwater rainbowfish and the implications for offspring morphology

2014 ◽  
Vol 65 (5) ◽  
pp. 400 ◽  
Author(s):  
D. Starrs ◽  
B. C. Ebner ◽  
S. M. Eggins ◽  
C. J. Fulton
Keyword(s):  
Stable Isotopes ◽  
Single Injection ◽  
Early Life History ◽  
Unintended Effects ◽  
Maternal Transmission ◽  
Stable Isotope Labelling ◽  
Physical Size ◽  
Isotope Labelling ◽  
Daily Spawning

Transgenerational marking is increasingly being used to study the early life history, biology and ecology of fishes. However, the timeframe over which the injected enriched stable isotopes remain in the mother and are passed onto her offspring is largely unknown. Similarly, we have relatively little knowledge of the effects of isotope labelling on the morphology of offspring. In this study, we injected adult female eastern rainbowfish (Melanotaenia splendida) with two doses (20 µg g–1 and 40 µg g–1) of enriched 137Ba or 87Sr stable isotopes to mark the otoliths of their larvae and examine the effects of isotope labelling on larvae morphology. Isotope ratios in larval otoliths were significantly different from controls in larvae hatched up to 174 days post-injection, indicating that enriched isotopes can mark the larvae of this daily spawning species up to 6 months after a single injection. Isotope-marked larvae displayed variable, but generally increased physical size, indicating that enriched stable isotope labelling may have some unintended effects on larvae morphology. Consequently, transgenerational marking provides a long-term tool for marking the offspring of M. splendida to disentangle their patterns of survivorship and dispersal, with the caveat that such studies should be interpreted in light of potential isotope-related changes in offspring morphology.

scholarly journals Tree Vitality and Forest Health: Can Tree-Ring Stable Isotopes Be Used as Indicators?

2021 ◽  
Author(s):  
Paolo Cherubini ◽  
Giovanna Battipaglia ◽  
John L. Innes
Keyword(s):  
Air Pollution ◽  
Stable Isotopes ◽  
Tree Ring ◽  
Forest Decline ◽  
Forest Health ◽  
Stress Factors ◽  
Tree Vitality ◽  
The Past ◽  
Physiological Processes

Abstract Purpose of Review Society is concerned about the long-term condition of the forests. Although a clear definition of forest health is still missing, to evaluate forest health, monitoring efforts in the past 40 years have concentrated on the assessment of tree vitality, trying to estimate tree photosynthesis rates and productivity. Used in monitoring forest decline in Central Europe since the 1980s, crown foliage transparency has been commonly believed to be the best indicator of tree condition in relation to air pollution, although annual variations appear more closely related to water stress. Although crown transparency is not a good indicator of tree photosynthesis rates, defoliation is still one of the most used indicators of tree vitality. Tree rings have been often used as indicators of past productivity. However, long-term tree growth trends are difficult to interpret because of sampling bias, and ring width patterns do not provide any information about tree physiological processes. Recent Findings In the past two decades, tree-ring stable isotopes have been used not only to reconstruct the impact of past climatic events, such as drought, but also in the study of forest decline induced by air pollution episodes, and other natural disturbances and environmental stress, such as pest outbreaks and wildfires. They have proven to be useful tools for understanding physiological processes and tree response to such stress factors. Summary Tree-ring stable isotopes integrate crown transpiration rates and photosynthesis rates and may enhance our understanding of tree vitality. They are promising indicators of tree vitality. We call for the use of tree-ring stable isotopes in future monitoring programmes.

Dynamic lipidomics with stable isotope labelling

2009 ◽  
Vol 877 (26) ◽  
pp. 2716-2721 ◽  
Author(s):  
Anthony D. Postle ◽  
Alan N. Hunt
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Labelling ◽  
Isotope Labelling

scholarly journals Stable isotope labelling in vivo as an aid to protein identification in peptide mass fingerprinting

PROTEOMICS ◽  
2002 ◽  
Vol 2 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Julie M. Pratt ◽  
Duncan H. L. Robertson ◽  
Simon J. Gaskell ◽  
Isabel Riba-Garcia ◽  
Simon J. Hubbard ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Protein Identification ◽  
Peptide Mass Fingerprinting ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Peptide Mass

scholarly journals Three distinct Holocene intervals of stalagmite deposition and nondeposition revealed in NW Madagascar, and their paleoclimate implications

2017 ◽  
Vol 13 (12) ◽  
pp. 1771-1790 ◽  
Author(s):  
Ny Riavo Gilbertinie Voarintsoa ◽  
Loren Bruce Railsback ◽  
George Albert Brook ◽  
Lixin Wang ◽  
Gayatri Kathayat ◽  
...  
Keyword(s):  
Stable Isotopes ◽  
High Resolution ◽  
Late Holocene ◽  
Intertropical Convergence Zone ◽  
Convergence Zone ◽  
High Resolution Data ◽  
The Holocene ◽  
Middle Holocene ◽  
Resolution Data

Abstract. Petrographic features, mineralogy, and stable isotopes from two stalagmites, ANJB-2 and MAJ-5, respectively from Anjohibe and Anjokipoty caves, allow distinction of three intervals of the Holocene in NW Madagascar. The Malagasy early Holocene (between ca. 9.8 and 7.8 ka) and late Holocene (after ca. 1.6 ka) intervals (MEHI and MLHI, respectively) record evidence of stalagmite deposition. The Malagasy middle Holocene interval (MMHI, between ca. 7.8 and 1.6 ka) is marked by a depositional hiatus of ca. 6500 years. Deposition of these stalagmites indicates that the two caves were sufficiently supplied with water to allow stalagmite formation. This suggests that the MEHI and MLHI intervals may have been comparatively wet in NW Madagascar. In contrast, the long-term depositional hiatus during the MMHI implies it was relatively drier than the MEHI and the MLHI. The alternating wet–dry–wet conditions during the Holocene may have been linked to the long-term migrations of the Intertropical Convergence Zone (ITCZ). When the ITCZ's mean position is farther south, NW Madagascar experiences wetter conditions, such as during the MEHI and MLHI, and when it moves north, NW Madagascar climate becomes drier, such as during the MMHI. A similar wet–dry–wet succession during the Holocene has been reported in neighboring locations, such as southeastern Africa. Beyond these three subdivisions, the records also suggest wet conditions around the cold 8.2 ka event, suggesting a causal relationship. However, additional Southern Hemisphere high-resolution data will be needed to confirm this.

scholarly journals Comparative strategies to induce long-term graft acceptance in fully allogeneic renal versus cardiac allograft models by CD28-B7 T cell costimulatory blockade: role of thymus and spleen.

1998 ◽  
Vol 9 (5) ◽  
pp. 891-898
Author(s):  
M Schaub ◽  
T H Stadlbauer ◽  
A Chandraker ◽  
J P Vella ◽  
L A Turka ◽  
...  
Keyword(s):  
T Cell ◽  
Species Differences ◽  
Single Injection ◽  
Maintenance Phase ◽  
Cardiac Allograft ◽  
Costimulatory Blockade ◽  
Histocompatibility Complex ◽  
Donor Cells

Blocking CD28-B7 T cell costimulatory activation by the fusion protein CTLA4Ig prevents rejection and induces long-term graft acceptance in various experimental transplant models. There are reported differences in the efficacy of CTLA4Ig in renal and cardiac rodent allograft models, but it is not clear whether these are due to the strain or species differences investigated in the different studies reported. This study investigates the effect of blocking CD28-B7 T cell costimulation with murine CTLA4Ig in rat models of acute renal and cardiac allograft rejection models, using the same complete major histocompatibility complex-incompatible strain combination. A single injection of murine CTLA4Ig 2 d after engraftment was able to induce long-term graft acceptance (> 100 d) in 54% of Lewis rat recipients of Wistar-Furth kidneys. Transferring this protocol into the acute Wistar-Furth to Lewis heart allograft model resulted in a mean graft survival time of 24.7+/-16.9 d, and all grafts were ultimately rejected. Only concomitant injection of donor cells (4 x 10(7) splenocytes) plus a single injection of CTLA4Ig on the day of transplant could induce long-term graft acceptance in 50% of animals. In both the cardiac and renal transplant models, the thymus and spleen were required for induction of tolerance. The maintenance phase of tolerance, however, did not require an intact thymus but did require the presence of a spleen. These data have important clinical applicability because human studies with T cell costimulatory blockade are being planned.

scholarly journals The contributions of biosynthesis and acyl chain remodelling to the molecular species profile of phosphatidylcholine in yeast

2005 ◽  
Vol 33 (5) ◽  
pp. 1146-1149 ◽  
Author(s):  
H.A. Boumann ◽  
A.I.P.M. de Kroon
Keyword(s):  
Stable Isotope ◽  
Species Composition ◽  
Molecular Species ◽  
Acyl Chain ◽  
Membrane Lipid ◽  
Molecular Species Composition ◽  
Stable Isotope Labelling ◽  
Membrane Function ◽  
Isotope Labelling ◽  
Acyl Chains

Phosphatidylcholine (PC) is a very abundant membrane lipid in most eukaryotes, including yeast. The molecular species profile of PC, i.e. the ensemble of PC molecules with acyl chains differing in number of carbon atoms and double bonds, is important for membrane function. Pathways of PC synthesis and turnover maintain PC homoeostasis and determine the molecular species profile of PC. Studies addressing the processes involved in establishing the molecular species composition of PC in yeast using stable isotope labelling combined with detection by MS are reviewed.

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