scholarly journals Large-scale recombinant protein production and structure-based drug design capabilities at CSIRO

2010 ◽  
Vol 31 (2) ◽  
pp. 77
Author(s):  
G Lovrecz ◽  
G Dumsday ◽  
T Adams
Keyword(s):  
Drug Design ◽  
Recombinant Protein ◽  
Collaborative Research ◽  
Recombinant Protein Production ◽  
Large Scale ◽  
Protein Production ◽  
Research Infrastructure ◽  
Structure Based Drug Design ◽  
Production Facilities

CSIRO has opened its large-scale Recombinant Protein Production Facilities (RPPF) as part of the National Collaborative Research Infrastructure Scheme (NCRIS).

Debottlenecking recombinant protein production in Bacillus megaterium under large-scale conditions-targeted precursor feeding designed from metabolomics

2012 ◽  
Vol 109 (6) ◽  
pp. 1538-1550 ◽  
Author(s):  
Claudia Korneli ◽  
Christoph Josef Bolten ◽  
Thibault Godard ◽  
Ezequiel Franco-Lara ◽  
Christoph Wittmann
Keyword(s):  
Recombinant Protein ◽  
Bacillus Megaterium ◽  
Recombinant Protein Production ◽  
Large Scale ◽  
Protein Production ◽  
Precursor Feeding

Large-Scale Insect Cell-Culture for Recombinant Protein Production

1988 ◽  
Vol 6 (12) ◽  
pp. 1406-1410 ◽  
Author(s):  
Brian Maiorella ◽  
Duane Inlow ◽  
Andrea Shauger ◽  
David Harano
Keyword(s):  
Cell Culture ◽  
Recombinant Protein ◽  
Insect Cell ◽  
Recombinant Protein Production ◽  
Large Scale ◽  
Protein Production ◽  
Scale Insect ◽  
Insect Cell Culture

scholarly journals Gene Delivery into Plant Cells for Recombinant Protein Production

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Qiang Chen ◽  
Huafang Lai
Keyword(s):  
Gene Delivery ◽  
Recombinant Protein ◽  
Recombinant Proteins ◽  
Recombinant Protein Production ◽  
Large Scale ◽  
Protein Production ◽  
Plant Cells ◽  
High Volume ◽  
Extensive Discussion ◽  
Transgene Delivery

Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms. In this paper, we review the recent progress in the methodology of agroinfiltration, a solution to overcome the challenge of transgene delivery into plant cells for large-scale manufacturing of recombinant proteins. General gene delivery methodologies in plants are first summarized, followed by extensive discussion on the application and scalability of each agroinfiltration method. New development of a spray-based agroinfiltration and its application on field-grown plants is highlighted. The discussion of agroinfiltration vectors focuses on their applications for producing complex and heteromultimeric proteins and is updated with the development of bridge vectors. Progress on agroinfiltration inNicotianaand non-Nicotianaplant hosts is subsequently showcased in context of their applications for producing high-value human biologics and low-cost and high-volume industrial enzymes. These new advancements in agroinfiltration greatly enhance the robustness and scalability of transgene delivery in plants, facilitating the adoption of plant transient expression systems for manufacturing recombinant proteins with a broad range of applications.

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