An integrated morphological–molecular approach reveals new insights on the systematics of the octocoral Telestula humilis (Thomson, 1927) (Octocorallia:Alcyonacea:Clavulariidae)

2021 ◽  
Author(s):  
Angelo Poliseno ◽  
Alvaro Altuna ◽  
Lara C. Puetz ◽  
Sarah S. T. Mak ◽  
Pilar Ríos ◽  
...  
Keyword(s):  
Deep Sea ◽  
Phylogenetic Analyses ◽  
Basal Part ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Approach ◽  
Gastric Cavity ◽  
28S Rrna Gene

Telestula humilis (Thomson, 1927) is a rare deep-sea stoloniferan octocoral distributed in the eastern Atlantic. Here we compared seven putative colonies of this species collected off Spain with the lectotype from the Oceanographic Museum of Monaco and found them to be identical morphologically. Phylogenetic analyses on both full mitogenomes and a concatenated alignment containing two mtDNA genes (mtMutS and Cox1) and nuclear 28S rRNA gene recovered Telestula humilis sister to Incrustatus and Inconstantia rather than to other species of Telestula. This therefore supports its taxonomic reassignment to Pseudotelestula gen. nov. as Pseudotelestula humilis comb. nov. The taxonomic reassignment is also supported by subtle differences observed between the morphology of the colony and the sclerome of Pseudotelestula humilis comb. nov. and the two sister genera. The occurrence of an intrusion tissue with sclerites in the basal part of the gastric cavity of the adult polyps is shared among Telestula and Pseudotelestula gen. nov. However, Pseudotelestula gen. nov. has sclerites arranged in a collaret and points below the tentacles, the sclerites of the calyx wall and the stolon are plump warty spindles, and the intrusion tissue has long sticks and spindles with cone-like spines.

Characterisation of populations of Heterodera trifolii Goffart, 1932 (Nematoda: Heteroderidae) in Japan and their phylogenetic relationships with closely related species

Nematology ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. 543-558
Author(s):  
Shigeyuki Sekimoto ◽  
Taketo Uehara ◽  
Takayuki Mizukubo
Keyword(s):  
Related Species ◽  
Phylogenetic Relationships ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Closely Related Species ◽  
28S Rrna Gene ◽  
Intraspecific Variations ◽  
Low Levels

The Japanese populations of the clover cyst nematode,Heterodera trifolii, were characterised morphologically, morphometrically and molecularly. The morphology and morphometrics of six Japanese populations ofH. trifoliiwere in congruence with those of previous descriptions of this species from Germany, Italy, Japan, New Zealand, Russia, The Netherlands, UK and USA. The results of the phylogenetic analyses of the D2-D3 expansion segments of 28S rRNA gene and theCOImtDNA gene sequences discriminatedH. trifoliifrom most of the other closely related species of theSchachtiigroup. The intraspecific sequence differences in theSchachtiigroup were much higher inCOIthan in D2-D3. TheH. trifoliipopulations displayed very low levels of intraspecific variations (up to 0.3%) in theCOImtDNA gene sequences. The phylogenetic relationships ofH. trifoliiwith otherHeteroderaspecies, as inferred from the analyses of the ITS rRNA gene, D2-D3 of 28S rRNA gene andCOImtDNA gene sequences, were determined.

scholarly journals Intra-species sequence variability in 28s rRNA gene of Oesophagostomum venulosum isolated from goats of West Bengal, India

2010 ◽  
Vol 3 (7) ◽  
pp. 515-518 ◽  
Author(s):  
Subhasish Bandyopadhyay ◽  
Asit Kumar Bera ◽  
Sourav Sikdar ◽  
Sumanta De ◽  
Subhashree Ghosh ◽  
...  
Keyword(s):  
West Bengal ◽  
Rrna Gene ◽  
28S Rrna ◽  
Sequence Variability ◽  
28S Rrna Gene

scholarly journals Phylogenetic Analysis of 28S rRNA Gene of Indigenous Xiphinema pachydermum

2019 ◽  
Vol 8 (07) ◽  
pp. 1960-1968
Author(s):  
Anju Sharma ◽  
Satish K. Sharma ◽  
Kiran Rana ◽  
Anil Kumar Verma
Keyword(s):  
Phylogenetic Analysis ◽  
Rrna Gene ◽  
28S Rrna ◽  
28S Rrna Gene

Morphological and molecular characterisation of Heterodera koreana (Vovlas, Lamberti & Choo, 1992) Mundo-Ocampo, Troccoli, Subbotin, Del Cid, Baldwin & Inserra, 2008 (Nematoda: Heteroderidae) from bamboo in Japan

Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 333-350 ◽  
Author(s):  
Shigeyuki Sekimoto ◽  
Taketo Uehara ◽  
Takayuki Mizukubo
Keyword(s):  
Phylogenetic Analyses ◽  
Original Description ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Phyllostachys Edulis ◽  
Relationship Of ◽  
First Time

The Korean cyst nematode, Heterodera koreana, was recorded for the first time from Japan and characterised morphologically, morphometrically and molecularly. In total, 41 populations were detected from soil samples collected from the rhizosphere of four bamboo species in Japan: 31 populations from moso bamboo (Phyllostachys edulis), seven from madake (P. bambusoides), two from henon bamboo (P. nigra var. henonis) and one from fish pole bamboo (P. aurea). The morphology and morphometrics of the Japanese population were in agreement with those of the original description of H. koreana from South Korea and other subsequent descriptions from China and Iran, with the exception of some minor differences. The results of the phylogenetic analyses of the D2-D3 expansion segments of 28S rRNA gene and ITS rRNA gene sequences confirmed the species identification and phylogenetic relationship of H. koreana with other Heterodera species. The COI mtDNA gene sequences were obtained for the first time for H. koreana. Three COI haplotypes found in Japanese H. koreana populations showed a characteristic geographical distribution in Japan.

scholarly journals Molecular and morphological characterisation of Xiphinema granatum n. sp. and Longidorus pisi Edward, Misra & Singh, 1964 (Dorylaimida: Longidoridae) from Iran

Nematology ◽  
2012 ◽  
Vol 14 (8) ◽  
pp. 949-960 ◽  
Author(s):  
Majid Pedram ◽  
Ebrahim Pourjam ◽  
Juan E. Palomares-Rius ◽  
Razieh Ghaemi ◽  
Carolina Cantalapiedra-Navarrete ◽  
...  
Keyword(s):  
New Species ◽  
Species Group ◽  
Rrna Gene ◽  
28S Rrna ◽  
Morphometric Data ◽  
Molecular Analyses ◽  
Morphological Characterisation ◽  
28S Rrna Gene ◽  
Close Relationship ◽  
Female Genital

Xiphinema granatum n. sp. from pomegranate in Saveh, Markazi province, central Iran, is described and illustrated. The new species belongs to species group 8 of the genus Xiphinema sensu Loof & Luc, 1990. The new species is characterised by a body 3.5-4.1 mm long, 37-49 μm diam., odontostyle and odontophore 118-132 and 65-74 μm long, respectively, presence of sperm in the female genital tracts, uterus devoid of any Z-differentiation or spines, and abundant males with spicules 45-58 μm long. The polytomous identification codes of the new species are: A4, B4, C5a, D5, E56, F4, G23, H2, I3, J4, K2, L2. Beside morphological and morphometric data, molecular analyses of D2-D3 fragments of 28S rRNA gene placed the new species in close relationship with X. vuittenezi and X. italiae and some species belonging to Xiphinema morphospecies group 6, but clearly separated from X. index. In addition, an Iranian population of Longidorus pisi from the same locality is morphologically and molecularly characterised.

scholarly journals Thermovibrio ammonificans sp. nov., a thermophilic, chemolithotrophic, nitrate-ammonifying bacterium from deep-sea hydrothermal vents

2004 ◽  
Vol 54 (1) ◽  
pp. 175-181 ◽  
Author(s):  
Costantino Vetriani ◽  
Mark D. Speck ◽  
Susan V. Ellor ◽  
Richard A. Lutz ◽  
Valentin Starovoytov
Keyword(s):  
Deep Sea ◽  
Hydrothermal Vents ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
East Pacific ◽  
Casamino Acids ◽  
The 16S Rrna Gene

A thermophilic, anaerobic, chemolithoautotrophic bacterium was isolated from the walls of an active deep-sea hydrothermal vent chimney on the East Pacific Rise at 9° 50′ N. Cells of the organism were Gram-negative, motile rods that were about 1·0 μm in length and 0·6 μm in width. Growth occurred between 60 and 80 °C (optimum at 75 °C), 0·5 and 4·5 % (w/v) NaCl (optimum at 2 %) and pH 5 and 7 (optimum at 5·5). Generation time under optimal conditions was 1·57 h. Growth occurred under chemolithoautotrophic conditions in the presence of H2 and CO2, with nitrate or sulfur as the electron acceptor and with concomitant formation of ammonium or hydrogen sulfide, respectively. Thiosulfate, sulfite and oxygen were not used as electron acceptors. Acetate, formate, lactate and yeast extract inhibited growth. No chemoorganoheterotrophic growth was observed on peptone, tryptone or Casamino acids. The genomic DNA G+C content was 54·6 mol%. Phylogenetic analyses of the 16S rRNA gene sequence indicated that the organism was a member of the domain Bacteria and formed a deep branch within the phylum Aquificae, with Thermovibrio ruber as its closest relative (94·4 % sequence similarity). On the basis of phylogenetic, physiological and genetic considerations, it is proposed that the organism represents a novel species within the newly described genus Thermovibrio. The type strain is Thermovibrio ammonificans HB-1T (=DSM 15698T=JCM 12110T).

Redescription of Cerebratulus marginatus auct. (Nemertea: Pilidiophora) from Hokkaido, Japan, as a new species

Zootaxa ◽  
2020 ◽  
Vol 4819 (2) ◽  
pp. 295-315
Author(s):  
HIROSHI KAJIHARA
Keyword(s):  
16S Rrna ◽  
Vascular System ◽  
Phylogenetic Analyses ◽  
Far East ◽  
Muscle Layer ◽  
The Body ◽  
Rrna Gene ◽  
28S Rrna ◽  
Anterior Portion ◽  
Longitudinal Muscle Layer

The heteronemertean Cerebratulus orochi sp. nov. is described based on material collected intertidally at a muddy beach in Akkeshi, northern Japan. For the last 80 years, the species has been confused with Cerebratulus marginatus Renier, 1804; the latter was originally described from the Adriatic and once believed to occur in many places in the northern hemisphere including Japan. Cerebratulus orochi sp. nov. is morphologically different from all the congeners including C. marginatus by the following combination of characters: several layers of diagonal-muscle meshwork coated with connective tissue, proximo-distally distributed in cross section from the distal portion of the body-wall outer longitudinal muscle layer to the cutis-gland zone throughout the anterior portion of the body from the precerebral to the foregut regions; the cephalic vascular system consisting of lateral and mid-dorsal vessels; and the sub-rhynchocoelic vessel possessing a pair of antero-lateral diverticula before the former forks posteriorly into a pair of lower lateral vessels in the post-cerebral, pre-oral region. Previous records of C. marginatus from Japanese waters are no longer considered to be substantiated. Multi-locus phylogenetic analyses based on the mitochondrial 16S rRNA and cytochrome c oxidase subunit I (COI), as well as the nuclear 18S rRNA, 28S rRNA, and histone H3 genes among heteronemerteans comprising the “Cerebratulus clade” indicated that C. orochi sp. nov. was closely related to C. cf. marginatus from the US Pacific coast. A MegaBLAST search at the NCBI website with the 16S rRNA gene sequence from C. orochi sp. nov. followed by a couple of species delimitation analyses suggests that larvae of the species are also distributed in Vostok Bay, Far East Russia.

Placement of Cacoceria and phylogenetic relationships of the xylotine genera of the tribe Milesiini (Diptera, Syrphidae: Eristalinae) based on molecular characters

Zootaxa ◽  
2006 ◽  
Vol 1171 (1) ◽  
pp. 17 ◽  
Author(s):  
GUNILLA STÅHLS
Keyword(s):  
Intraspecific Variation ◽  
Phylogenetic Relationships ◽  
Rrna Gene ◽  
28S Rrna ◽  
Large Fragment ◽  
Mitochondrial Coi ◽  
Phylogenetic Placement ◽  
28S Rrna Gene ◽  
Molecular Characters

The phylogenetic relationships of traditional xylotine genera of tribe Milesiini (Diptera, Syrphidae: Eristalinae) were explored using molecular character of a large fragment of the mitochondrial COI and the D2-3 region of the nuclear ribosomal 28S rRNA gene. Of particular interest was the phylogenetic placement of the enigmatic Neotropical genus Cacoceria. The ingroup included 48 taxa, and Cheilosia illustrata (Rhingiini, Eristalinae) was used as outgroup. Multiple specimens of more common taxa were sequenced for surveying levels of intraspecific variation. The combined dataset was analysed using parsimony and optimisation alignment, using the program POY. Cacoceria was resolved within the Chalcosyrphus clade. Earlier hypotheses placed the taxon in the tribe Myoleptini or Chrysogasterini, or in Xylotini without stating a closer relationship to any particular xylotine taxon. The representatives of traditional xylotine taxa were resolved as ((Hadromyia + (Brachypalpoides + Blera + Lejota) + (Xylota sg. Hovaxylota + Xylota sg. Sterphoides) + (Xylota (including Sterphus)) while Neplas and Brachypalpus were resolved in the Myoleptini albeit with very low support.

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