A revision of Peronina Plate, 1893 (Gastropoda : Euthyneura : Onchidiidae) based on mitochondrial and nuclear DNA sequences, morphology and natural history

2018 ◽  
Vol 32 (4) ◽  
pp. 803 ◽  
Author(s):  
Tricia C. Goulding ◽  
Shau Hwai Tan ◽  
Siong Kiat Tan ◽  
Deepak Apte ◽  
Vishal Bhave ◽  
...  
Keyword(s):  
Natural History ◽  
Dna Sequences ◽  
Nuclear Dna ◽  
Comparative Anatomy ◽  
Integrative Approach ◽  
Mangrove Forests ◽  
Test Species ◽  
Mitochondrial Coi ◽  
Male Copulatory Apparatus ◽  
Strait Of Malacca

Peronina Plate, 1893 is a genus of onchidiids that live on the mud in mangrove forests. Peronina can be identified in the field by the lung opening at the margin between the ventral hyponotum and the dorsal notum, and by the distinctive scalloped notum edge. This genus was previously known only from the holotype of the type species, Peronina alta Plate, 1893, from eastern India. Onchidium tenerum Stoliczka, 1869 is moved to Peronina and applies to the same species as Peronina alta. Peronina species are described using an integrative approach (natural history, comparative anatomy and DNA sequences). Mitochondrial COI and 16S sequences and nuclear ITS2 and 28S sequences are used to independently test species boundaries. Mitochondrial sequences yielded three units separated by a large barcode gap, but nuclear sequences yielded two units. Because these two units are congruent with differences in the male copulatory apparatus, they are accepted as species. Explanations for highly divergent COI haplotypes within one species are discussed. Peronina tenera (Stoliczka, 1869) is distributed in the Bay of Bengal and the Strait of Malacca, while P. zulfigari Goulding & Dayrat, sp. nov. is endemic to the Strait of Malacca. The two species differ internally but are cryptic externally.

scholarly journals Systematic revision of Platevindex Baker, 1938 (Gastropoda: Euthyneura: Onchidiidae)

2021 ◽  
Vol 737 ◽  
pp. 1-133
Author(s):  
Tricia C. Goulding ◽  
Adam J. Bourke ◽  
Joseph Comendador ◽  
Munawar Khalil ◽  
Ngo Xuan Quang ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Nuclear Dna ◽  
New Caledonia ◽  
Comparative Anatomy ◽  
Integrative Approach ◽  
Mangrove Forests ◽  
Type Specimens ◽  
Systematic Revision ◽  
West Pacific ◽  
Nomina Dubia

In the Indo-West Pacific, intertidal slugs of the genus Platevindex Baker, 1938 are common in mangrove forests, where they typically live on the roots and trunks of mangrove trees. These slugs are easily distinguished from most onchidiids by their hard notum and narrow foot, but despite their large size and abundance, species diversity and geographic distributions have remained a mystery. With the aid of new collections from across the entire Indo-West Pacific, the taxonomy of Platevindex is revised using an integrative approach (natural history field observations, re-examination of type specimens, mitochondrial and nuclear DNA sequences, and comparative anatomy). In this monograph, nine species of Platevindex are recognized, including one new to science: P. amboinae (Plate, 1893), P. applanatus (Simroth, 1920) comb. nov., P. aptei Goulding & Dayrat sp. nov., P. burnupi (Collinge, 1902) comb. nov., P. coriaceus (Semper, 1880), P. latus (Plate, 1893), P. luteus (Semper, 1880), P. martensi (Plate, 1893) and P. tigrinus (Stoliczka, 1869) comb. nov. Five species names are recognized as junior synonyms, four of which are new, and two Platevindex names are regarded as nomina dubia. One new subspecies is also recognized: P. coriaceus darwinensis Goulding & Dayrat subsp. nov. Most species were previously known only from the type material and many new geographic records are provided across the Indo-West Pacific, from South Africa to the West Pacific (Japan, New Ireland and New Caledonia).

scholarly journals A new species and new records of Onchidium slugs (Gastropoda, Euthyneura, Pulmonata, Onchidiidae) in South-East Asia

ZooKeys ◽  
2019 ◽  
Vol 892 ◽  
pp. 27-57 ◽  
Author(s):  
Benoît Dayrat ◽  
Tricia C. Goulding ◽  
Munawar Khalil ◽  
Deepak Apte ◽  
Shau Hwai Tan
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
New Records ◽  
Comparative Anatomy ◽  
Peninsular Malaysia ◽  
Mitochondrial Coi ◽  
Distinct Color ◽  
First Time ◽  
A New Species ◽  
Strait Of Malacca

A new species, Onchidium melakense Dayrat & Goulding, sp. nov., is described, bringing the total to four known species in the genus Onchidium Buchannan, 1800. Onchidium melakense is a rare species with only nine individuals found at three mangrove sites in the Andaman Islands and the Strait of Malacca (western Peninsular Malaysia and eastern Sumatra). The new species is delineated based on mitochondrial (COI and 16S) and nuclear (ITS2 and 28S) DNA sequences as well as comparative anatomy. Each Onchidium species is characterized by a distinct color and can easily be identified in the field, even in the Strait of Malacca where there are three sympatric Onchidium species. An identification key is provided. In addition, Onchidium stuxbergi (Westerlund, 1883) is recorded for the first time from eastern Sumatra, and Onchidium pallidipes Tapparone-Canefri, 1889, of which the type material is described and illustrated here, is regarded as a new junior synonym of O. stuxbergi.

scholarly journals Integrative taxonomy of a new and highly-diverse genus of onchidiid slugs from the Coral Triangle (Gastropoda, Pulmonata, Onchidiidae)

ZooKeys ◽  
2018 ◽  
Vol 763 ◽  
pp. 1-111 ◽  
Author(s):  
Tricia C. Goulding ◽  
Munawar Khalil ◽  
Shau Hwai Tan ◽  
Benoît Dayrat
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Comparative Anatomy ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
The Philippines ◽  
Coral Triangle ◽  
Yellow Orange

A new genus of onchidiid slugs,WallaconchisGoulding & Dayrat,gen. n., is described, including ten species. Five species were previously described but known only from the type material:Wallaconchisater(Lesson, 1830),W.graniferum(Semper, 1880),W.nangkauriense(Plate, 1893),W.buetschlii(Stantschinsky, 1907), andW.gracile(Stantschinsky, 1907), all of which were originally classified inOnchidiumBuchannan, 1800. Many new records are provided for these five species, which greatly expand their known geographic distributions. Five species are new:WallaconchisachleitneriGoulding,sp. n.,W.comendadoriGoulding & Dayrat,sp. n.,W.melanesiensisGoulding & Dayrat,sp. n.,W.sinanuiGoulding & Dayrat,sp. n., andW.uncinusGoulding & Dayrat,sp. n.Nine of the tenWallaconchisspecies are found in the Coral Triangle (eastern Indonesia and the Philippines). Sympatry is high, with up to six species found on the island of Bohol (Philippines) and eight species overlapping in northern Sulawesi (Indonesia).Wallaconchisis distinguished from other onchidiids by its bright dorsal colors (red, yellow, orange) but those are extremely variable and not useful for specific identification. Internally, the reproductive system can be used to identify allWallaconchisspecies. The copulatory organs ofWallaconchisspecies are especially diverse compared to other onchidiid genera, and the possible role of reproductive incompatibility in species diversification is discussed. All specimens examined were freshly collected for the purpose of a worldwide revision of the Onchidiidae Rafinesque, 1815. The species are well delineated using DNA sequences and comparative anatomy. Mitochondrial DNA analysis yields thirteen molecular units separated by a large barcode gap, while nuclear DNA yields nine units. By integrating nuclear DNA and mitochondrial DNA with morphology, ten species are recognized. The natural history of each species (e.g., the microhabitat where they are found) is also documented. Nomenclature is addressed thoroughly (the types of all onchidiid species were examined, lectotypes were designated when needed,nomina dubiaare discussed). Morphological characters, transitions to new microhabitats, and diversification processes are discussed in the context of a robust molecular phylogeny.

Taxonomy of rock-wallabies, Petrogale (Marsupialia: Macropodidae). IV. Multifaceted study of the brachyotis group identifies additional taxa

2014 ◽  
Vol 62 (5) ◽  
pp. 401 ◽  
Author(s):  
Sally Potter ◽  
Robert L. Close ◽  
David A. Taggart ◽  
Steven J. B. Cooper ◽  
Mark D. B. Eldridge
Keyword(s):  
Dna Sequences ◽  
Nuclear Dna ◽  
Sensu Stricto ◽  
Northern Territory ◽  
Morphological Data ◽  
Integrative Approach ◽  
Morphological Evidence ◽  
Separate Species ◽  
Phylogenetic Structure ◽  
True Diversity

Defining taxonomic units is an important component of understanding how biodiversity has formed, and in guiding efforts to sustain it. Understanding patterns of biodiversity across the monsoonal tropics of northern Australia is limited, with molecular technology revealing deep phylogenetic structure and complex evolutionary histories. The brachyotis group of rock-wallabies (Petrogale spp.), which currently consists of three species (Petrogale brachyotis, P. burbidgei and P. concinna) distributed across north-western Australia, provides an example where current taxonomy does not reflect the true diversity or phylogenetic relationships within the group. We have used an integrative approach, combining morphological data, together with DNA sequences (~1000 bp mitochondrial DNA; ~3000 bp nuclear DNA) to resolve relationships within P. brachyotis. Phylogenetic and morphological analyses indicated that P. brachyotis (sensu lato) represents at least two separate species: P. brachyotis (sensu stricto) from the Kimberley and western Northern Territory, and P. wilkinsi from the northern and eastern Northern Territory. Petrogale brachyotis (sensu stricto) can be separated on genetic and morphological evidence into two subspecies: P. b. brachyotis and P. b. victoriae (subsp. nov.). Distinct genetic lineages have also been identified within both P. brachyotis and P. wilkinsi, as well as within P. burbidgei and P. concinna.

scholarly journals A new genus of air-breathing marine slugs from South-East Asia (Gastropoda, Pulmonata, Onchidiidae)

ZooKeys ◽  
2019 ◽  
Vol 877 ◽  
pp. 31-80
Author(s):  
Benoît Dayrat ◽  
Tricia C. Goulding ◽  
Munawar Khalil ◽  
Joseph Comendador ◽  
Quảng Ngô Xuân ◽  
...  
Keyword(s):  
New Species ◽  
East Asia ◽  
Dna Sequences ◽  
New Genus ◽  
Nuclear Dna ◽  
Comparative Anatomy ◽  
The Philippines ◽  
South East Asia ◽  
Air Breathing ◽  
Two New Species

As part of an ongoing effort to revise the taxonomy of air-breathing, marine, onchidiid slugs, a new genus, Laspionchis Dayrat & Goulding, gen. nov., is described from the mangroves of South-East Asia. It includes two new species, Laspionchis boucheti Dayrat & Goulding, sp. nov., and Laspionchis bourkei Dayrat & Goulding, sp. nov., both distributed from the Malacca Strait to the Philippines and Australia. This study is based on extensive field work in South-East Asia, comparative anatomy, and both mitochondrial (COI and 16S) and nuclear (ITS2 and 28S) DNA sequences. The two new species are found in the same habitat (mud surface in mangrove forests) and are externally cryptic but are distinct anatomically. Both species are also strongly supported by DNA sequences. Three cryptic, least-inclusive, reciprocally-monophyletic units within Laspionchis bourkei are regarded as subspecies: L. bourkei bourkei Dayrat & Goulding, ssp. nov., L. bourkei lateriensis Dayrat & Goulding, ssp. nov., and L. bourkei matangensis Dayrat & Goulding, ssp. nov. The present contribution shows again that species delineation is greatly enhanced by considering comparative anatomy and nuclear DNA sequences in addition to mitochondrial DNA sequences, and that thorough taxonomic revisions are the best and most efficient path to accurate biodiversity knowledge.

A new species of Brevipalpus (Acari: Tenuipalpidae) from the Azores Islands, with remarks on the B. cuneatus species group

2019 ◽  
Vol 24 (11) ◽  
pp. 2184-2208
Author(s):  
Jorge Laerson Dos Santos Alves ◽  
Francisco Ferragut ◽  
Renata Santos Mendonça ◽  
Aline Daniele Tassi ◽  
Denise Navia
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Nuclear Dna ◽  
Phylogenetic Analyses ◽  
Species Group ◽  
Wild Plants ◽  
Integrative Approach ◽  
Electron And Light Microscopy ◽  
Definition Of ◽  
A New Species

Brevipalpus contains about 290 species, some of which are considered of economic importance. In spite of their agricultural importance, the species diversity is scarcely known in several regions around the world, notably on wild plants. In this work a new species collected on an endemic ivy of the Azores Archipelago, Hedera azorica Carrière (Araliaceae), is described based on an integrative approach using morphological traits of the adults, obtained through electron and light microscopy, and molecular characters based on mitochondrial and nuclear DNA sequences. Phylogenetic analyses were conducted in order to produce reliable phylogenetic placement of the new species, which is tentatively classified in the B. portalis species group. Morphological similarities between the new species and B. cuneatus Canestrini and Fanzago point out to some inconsistencies in the current morphological classification of Brevipalpus species, especially in the definition of the B. cuneatus group, herein discussed.

scholarly journals Phylogenetic relationships of Cranichidinae and Prescottiinae (Orchidaceae, Cranichideae) inferred from plastid and nuclear DNA sequences

2009 ◽  
Vol 104 (3) ◽  
pp. 403-416 ◽  
Author(s):  
Gerardo A. Salazar ◽  
Lidia I. Cabrera ◽  
Santiago Madriñán ◽  
Mark W. Chase
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Nuclear Dna
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