Integrative systematics of clathrinid sponges: morphological, reproductive and phylogenetic characterisation of a new species of Leucetta from Antarctica (Porifera, Calcarea, Calcinea) with notes on the occurrence of flagellated sperm

2018 ◽  
Vol 32 (4) ◽  
pp. 827 ◽  
Author(s):  
Ana Riesgo ◽  
Fernanda F. Cavalcanti ◽  
Nathan J. Kenny ◽  
Pilar Ríos ◽  
Javier Cristobo ◽  
...  

Our study reports on the occurrence of a new species of Leucetta (Calcinea, Calcarea) from the Southern Ocean, Leucetta giribeti, sp. nov., collected in the shallow waters (15m depth) of Deception Island, South Shetland Islands. This new taxon is described based on a combination of morphological and molecular data, including the description of oocytes, embryos, larvae and sperm found in the choanosome. While female reproductive elements showed great similarities with those of other calcineans, sperm is reported here for the first time in the whole Calcinea subclass. Sperm cells are flagellated and possess a typical spermatic mid-piece, which is usually observed in cnidarians. In our phylogenetic analyses, we recovered Leucetta giribeti, sp. nov. as sister species of a clade formed by species of the genera Leucetta, Pericharax and Leucettusa. Although the clade in which Leucetta giribeti, sp. nov. is placed is supported by molecular and morphological features, we cannot propose a new genus due to uncertainties regarding the type species of the genus, Leucetta primigenia Haeckel, 1872. Our study reinforces the relevance of integrative approaches in the description of new taxa and contributes to resolving the poorly known reproductive patterns of Antarctic sponge species.

Phytotaxa ◽  
2018 ◽  
Vol 348 (2) ◽  
pp. 99 ◽  
Author(s):  
SHIKE HUANG ◽  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
RAJESH JEEWON ◽  
D JAYARAMA BHAT ◽  
RUNGTIWA PHOOKAMSAK ◽  
...  

In this paper, we report a new asexual-morph taxon belonging to the family Cordycipitaceae (Hypocreales), which is associated with a sexual morph of an ophioceras-like taxon on submerged wood collected from Baoshan, Yunnan, China. Morphologically, this new taxon is similar to known asexual morphs of Lecanicillium and characterized by conidiophores arising from hyaline hyphae, with gregarious, ellipsoid to ovoid, aseptate conidia. Phylogenetic analyses of a combined LSU, SSU, tef1-α and ITS sequence dataset positions our taxon in Cordycipitaceae and close to Lecanicillium sp. (CBS 639.85) and L. primulinum. Lecanicillium subprimulinum is introduced as a new species with support from molecular data.


Phytotaxa ◽  
2021 ◽  
Vol 484 (3) ◽  
pp. 291-297
Author(s):  
XINYU XU ◽  
CHANG-CHUN DING ◽  
WENQI HU ◽  
XIA YU ◽  
YU ZHENG ◽  
...  

A new species of Cymbidium (Orchidaceae), Cymbidium xichouense, from Yunnan Province, China, is described and illustrated based on morphological evidence and molecular analyses. The new orchid is morphologically similar to C. qinbeiense, but it has several morphological features that distinguish it from C. qiubeinense and all other recognized species in Cymbidium. Phylogenetic analyses based on nuclear (ITS) and plastid DNA (matK) were conducted, and the results also supported the status of C. xichouense as a new species, which is sister to C. qiubeiense.


Phytotaxa ◽  
2016 ◽  
Vol 283 (1) ◽  
pp. 43 ◽  
Author(s):  
BING LIU ◽  
YUN-QING HAO ◽  
YONG YANG ◽  
TIAN-TIAN SUN ◽  
YANG-JUN LAI ◽  
...  

Based on field investigations, morphological and molecular systematic studies, a new species, Caltha dysosmoides (Ranunculaceae) from southwestern China is described. It differs from all other known congeneric species by the densely dentate leaf margin, the pendulous pedicels, the scarlet flowers, the compressed filaments, and the triangular connective. To better understand taxonomy of this new species, phylogenetic analyses were conducted using a combined dataset from nrITS, plastid trnL-trnF region, and atpB-rbcL spacer. The results indicate that Caltha is monophyletic and C. dysosmoides is sister to C. sinogracilis. The conservation status of the new species is categorized as CR based on IUCN criteria.


2006 ◽  
Vol 37 (3) ◽  
pp. 281-300 ◽  
Author(s):  
Gerasimos Cassis ◽  
Jose Isidro Martinez-Cascales ◽  
Juan Antonio Sanchez

AbstractA new species of the plantbug genus Dicyphus (Insecta: Heteroptera: Miridae), D. umbertae Sanchez & Cassis sp. n., from Portugal is described and the closely related species, Dicyphus cerastii Wagner is redescribed. Several body measurements were taken. Male and female genitalia of both species are illustrated. D. umbertae was found in several localities in Central and South of Portugal on tomato (Lycopersicon esculentum Mill.) and Hyosciamus albus L. D. cerastii was found in southeast Spain on squash (Cucurbita maxima Duchesne), L. esculentum, Ononis natrix L., Withania frutescens (L.) Pauquy and H. albus. The external morphology of the two species is very alike and measurements overlap. In the two species the aedeagus has a similar pair of prominent slightly arcuate sclerites, but the shape and setae on the left paramere are different. Phylogenetic analyses were performed using 381 bp fragments of Cytochrome b. Other dicyphine species were used as outgroups in the analyses: Dicyphus tamaninii Wagner, D. rubicundus Blöte, D. escalerae Lindberg, Nesidiocoris tenuis (Reuter), Cyrtopeltis geniculata Fieber and Macrolophus sp. Several evolutionary models were explored under a maximum likelihood framework. The molecular analyses provided strong support for the species identity of the two sister species D. umbertae and D. cerastii.


Phytotaxa ◽  
2021 ◽  
Vol 509 (1) ◽  
Author(s):  
WENQI HU ◽  
QINGHAI ZHANG ◽  
GUIZHEN CHEN ◽  
MENGJIA ZHU ◽  
XIA YU ◽  
...  

This study describes a new orchid species, Cymbidium motuoense, from Xizang, China based on morphological and molecular analyses. Several unique morphological features distinguish this new orchid from all other species of Cymbidium. Based on its morphology, this orchid is similar to C. tracyanum but differs in its yellow-green flower with dark purple-red spots on the veins, oblong-elliptic sepals, side lobes of the lip with reddish brown hairs along the veins and disc with three purple-red lamellae. Phylogenetic analyses based on plastid DNA (matK and rbcL) supported C. motuoense as a new species. However, the low support in nuclear ribosomal ITS tree provide the possibility that the new orchid may be a natural hybrid of C. tracyanum and C. iridioides or another closely related species.


Zootaxa ◽  
2019 ◽  
Vol 4551 (5) ◽  
pp. 556 ◽  
Author(s):  
TATIANA KORSHUNOVA ◽  
RAHUL MEHROTRA ◽  
SPENCER ARNOLD ◽  
KENNET LUNDIN ◽  
BERNARD PICTON ◽  
...  

An integrative molecular and morphological study is presented for the family Unidentiidae. Molecular phylogenetic analyses were conducted with the inclusion of all previous and newly obtained molecular data for the family Unidentiidae Millen & Hermosillo 2012. A new species of the genus Unidentia Millen & Hermosillo 2012, U. aliciae sp. nov., is described from Thailand as part of an inventory of sea slugs at Koh Tao. All up-to-date available morphological data for the species of the genus Unidentia is for the first time summarized. Morphological differences among the different species of Unidentia are clarified showing that every species has its own distinguishable morphological traits. According to the new molecular and morphological data, the family Unidentiidae is re-confirmed as a well-supported taxon of the aeolidacean nudibranchs. The taxonomy and phylogeny of the Aeolidacea in the light of the family Unidentiidae is briefly discussed and necessity of a fine-scale and narrowly-defined taxa approach instead of a ‘‘superlumping’’ one is highlighted. 


PhytoKeys ◽  
2019 ◽  
Vol 130 ◽  
pp. 75-84
Author(s):  
Yun-Feng Huang ◽  
Li-Na Dong ◽  
Wei‑Bin Xu

Lysimachia fanii, a new species of Lysimachia (Subgen. Idiophyton, Primulaceae), is described and illustrated from Guangxi, China based on morphological and molecular data. Lysimachia fanii differs from L. verbascifolia, L. rupestris and L. alpestris mainly by the habit being nearly rosulate, leaves congested at the apex of the rhizome, leaf blades spatulate to narrowly oblanceolate and flowers solitary. Phylogenetic analyses supported L. verbascifolia as sister to L. fanii. This new species is endemic to limestone areas in Liucheng county of Guangxi, China.


Zootaxa ◽  
2021 ◽  
Vol 5005 (3) ◽  
pp. 291-303
Author(s):  
INGRID COSTA MARÇAL ◽  
FERNANDA POLLI PÁEZ ◽  
LENICE SOUZA-SHIBATTA ◽  
SILVIA HELENA SOFIA ◽  
GUSTAVO MONTEIRO TEIXEIRA

A new species of freshwater anomuran crab, Aegla buenoi n. sp., is described. The new taxon was collected from two streams within the Cinzas River basin, Paraná state, Brazil. We used morphological and molecular data (COI mtDNA) to distinguish the new species from its congeners. Aegla buenoi n. sp. is differentiated by morphological diagnostic features of the cephalothorax, chelipeds, second abdominal epimeron, and uropods. Molecular results confirm the separation of A. buenoi n. sp. from closely related species (A. castro Schmitt, 1942, A. lata Bond-Buckup & Buckup, 1994, and A. jacutinga Marçal & Teixeira, 2020). Hence, our study increases the known diversity of aeglids and reports the first species of Aegla from the Cinzas River basin.


2019 ◽  
Vol 95 (2) ◽  
pp. 507-516
Author(s):  
Pâmella Silva de Brito ◽  
Erick Cristofore Guimarães ◽  
Luis Fernando Carvalho-Costa ◽  
Felipe Polivanov Ottoni

A new species of Aphyocharax is described from the Maracaçumé river basin, eastern Amazon, based on morphological and molecular data. The new species differs from all its congeners, mainly by possessing the upper caudal-fin lobe longer than the lower one in mature males, and other characters related to teeth counts, colour pattern, and body depth at dorsal-fin origin. In addition, the new species is corroborated by a haplotype phylogenetic analyses based on the Cytochrome B (Cytb) mitochondrial gene, where its haplotypes are grouped into an exclusive lineage, supported by maximum posterior probability value, a species delimitation method termed the Wiens and Penkrot analysis (WP).


Zootaxa ◽  
2020 ◽  
Vol 4722 (5) ◽  
pp. 401-422 ◽  
Author(s):  
TRUONG QUANG NGUYEN ◽  
CUONG THE PHAM ◽  
TAO THIEN NGUYEN ◽  
ANH MAI LUONG ◽  
THOMAS ZIEGLER

A new species of Megophrys is described from Cao Bang Province, northeastern Vietnam based on morphological and molecular data. Morphologically, the new species is distinguishable from its congeners by a combination of the following characters: Size small (SVL 34.9–38.9 mm in males); tympanum visible; vomerine teeth absent; tongue not notched posteriorly; male with a single vocal sac; toes with rudimentary webbing; subarticular tubercles and lateral fringes absent on all digits; nuptial pads present on fingers I and II in males, with spicules; dorsal skin with scattered granules and tubercles; flank with tubercles; dorsum with a X-shaped dorsal ridge; dorsolateral folds prominent; a small horn-like tubercle present at the outer edge of the eyelid; dorsal surface yellowish brown with a dark brown triangle between the eyes, and a dark brown marking along the X-shaped ridge on the back. In the phylogenetic analyses, the new species is unambiguously nested within the subgenus Panophrys with interspecific uncorrected genetic p-distances (16S rRNA gene) varying from 4.26% (compared with M. rubrimera) to 10.80% (compared with M. acuta).


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