New insights into the effect of NdhO levels on cyanobacterial cell death triggered by high temperature

2021 ◽  
Author(s):  
Jiaohong Zhao ◽  
Yuanyuan Jiang ◽  
Yuhao Tian ◽  
Jun Mao ◽  
Lanzhen Wei ◽  
...  
Keyword(s):  
Cell Death ◽  
High Temperature ◽  
Cyanobacterial Cell

scholarly journals Yeast Nap1-Binding Protein Nbp2p Is Required for Mitotic Growth at High Temperatures and for Cell Wall Integrity

Genetics ◽  
2003 ◽  
Vol 165 (2) ◽  
pp. 517-529
Author(s):  
Kentaro Ohkuni ◽  
Asuko Okuda ◽  
Akihiko Kikuchi
Keyword(s):  
Cell Death ◽  
Cell Wall ◽  
High Temperature ◽  
High Temperatures ◽  
Hybrid System ◽  
Mapk Pathway ◽  
Binding Protein ◽  
Cell Wall Integrity ◽  
Calcofluor White ◽  
Two Hybrid

AbstractNbp2p is a Nap1-binding protein in Saccharomyces cerevisiae identified by its interaction with Nap1 by a two-hybrid system. NBP2 encodes a novel protein consisting of 236 amino acids with a Src homology 3 (SH3) domain. We showed that NBP2 functions to promote mitotic cell growth at high temperatures and cell wall integrity. Loss of Nbp2 results in cell death at high temperatures and in sensitivity to calcofluor white. Cell death at high temperature is thought not to be due to a weakened cell wall. Additionally, we have isolated several type-2C serine threonine protein phosphatases (PTCs) as multicopy suppressors and MAP kinase-kinase (MAPKK), related to the yeast PKC MAPK pathway, as deletion suppressors of the nbp2Δ mutant. Screening for deletion suppressors is a new genetic approach to identify and characterize additional proteins in the Nbp2-dependent pathway. Genetic analyses suggested that Ptc1, which interacts with Nbp2 by the two-hybrid system, acts downstream of Nbp2 and that cells lacking the function of Nbp2 prefer to lose Mkk1, but the PKC MAPK pathway itself is indispensable when Nbp2 is deleted at high temperature.

scholarly journals Rapidly Improving High Light and High Temperature Tolerances of Cyanobacterial Cell Factories Through the Convenient Introduction of an AtpA-C252F Mutation

2021 ◽  
Vol 12 ◽  
Author(s):  
Shanshan Zhang ◽  
Sini Zheng ◽  
Jiahui Sun ◽  
Xuexia Zeng ◽  
Yangkai Duan ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
High Temperature ◽  
Solar Energy ◽  
High Light ◽  
Cell Factory ◽  
Cyanobacterial Cell ◽  
Cell Factories ◽  
Green Production ◽  
Free Mode ◽  
Pcc 7942

Photosynthetic biomanufacturing is a promising route for green production of biofuels and biochemicals utilizing carbon dioxide and solar energy. Cyanobacteria are important microbial platforms for constructing photosynthetic cell factories. Toward scaled outdoor cultivations in the future, high light and high temperature tolerances of cyanobacterial chassis strains and cell factories would be determinant properties to be optimized. We proposed a convenient strategy for rapidly improving high light and high temperature tolerances of an important cyanobacterial chassis Synechococcus elongatus PCC 7942 and the derived cell factories. Through introduction and isolation of an AtpA-C252F mutation, PCC 7942 mutants with improved high light and high temperature tolerances could be obtained in only 4 days with an antibiotics-free mode. Adopting this strategy, cellular robustness and sucrose synthesizing capacities of a PCC 7942 cell factory were successfully improved.

Cortisol signaling and stress-induced gene expressions in response to copper toxicity through heat stress during zebrafish embryogenesis

2021 ◽  
Author(s):  
Kiyun Park ◽  
Ihn-Sil Kwak
Keyword(s):  
Cell Death ◽  
Heat Stress ◽  
High Temperature ◽  
Copper Toxicity ◽  
B Cell Lymphoma ◽  
Immune Defense ◽  
Gene Expressions ◽  
Transcriptional Responses ◽  
Stress Related Genes ◽  
Zebrafish Embryogenesis

Abstract Climate change is leading to an increase in temperatures, which has a stressful impact on the aquatic environment. Cortisol signaling is involved in enhancing metabolic processes such as anti-oxidation, immune defense, and osmoregulation, under stress conditions in fish. The present study aimed at evaluating the effects of copper (Cu) toxicity along with an increase in temperature during zebrafish embryogenesis, based on the transcriptional responses of cortisol and stress-related genes. A decreased survival rate was observed following combined exposure to high temperature and Cu. Heart rates of zebrafish embryos significantly increased only during heat stress. An abnormal morphology was induced by exposure to a combination of Cu and heat stress. Furthermore, heat stress also triggered Cu-induced intracellular reactive oxygen species production with upregulation of superoxide dismutase (SOD) and glutathione s-transferase (GST) and cell death with modified expressions of p53 and B-cell lymphoma-2 (Bcl-2) in the zebrafish embryo. Finally, increased cortisol level and altered expressions of cortisol-signaling genes were observed following exposure to Cu and high temperature. These results highlight that the realistic exposure to combined stressors disturbs cortisol-related defense pathways as well as the stress-induced processes of anti-oxidation and cell death in fish.

scholarly journals DNA damage and reactive oxygen species cause cell death in the rice local lesions 1 mutant under high light and high temperature

2019 ◽  
Vol 222 (1) ◽  
pp. 349-365 ◽  
Author(s):  
Zhennan Qiu ◽  
Li Zhu ◽  
Lei He ◽  
Dongdong Chen ◽  
Dali Zeng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Cell Death ◽  
High Temperature ◽  
Reactive Oxygen ◽  
High Light ◽  
Oxygen Species ◽  
Local Lesions

scholarly journals Integrin Alpha 7 Interacts with High Temperature Requirement A2 (HtrA2) to Induce Prostate Cancer Cell Death

2010 ◽  
Vol 177 (3) ◽  
pp. 1176-1186 ◽  
Author(s):  
Ze-Hua Zhu ◽  
Yan P. Yu ◽  
Zhong-Liang Zheng ◽  
Yang Song ◽  
Guo-Sheng Xiang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
High Temperature ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Death ◽  
Temperature Requirement ◽  
Alpha 7

scholarly journals Autophagy Mitigates High-Temperature Injury in Pollen Development of Arabidopsis thaliana

2016 ◽  
Author(s):  
Gonul Dundar ◽  
Zhenhua Shao ◽  
Nahoko Higashitani ◽  
Mami Kikuta ◽  
Masanori Izumi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
High Temperature ◽  
Pollen Development ◽  
Anther Wall ◽  
List Type ◽  
Male Sterile ◽  
Cellular Processes ◽  
Cellular Components ◽  
Plant Pollen

AbstractAutophagy is one of the cellular processes that break down cellular components during senescence, starvation, and stress. The susceptibility of plant pollen development to high-temperature (HT) stress is well known, but the involvement of autophagy in HT injury is yet to be clarified. Here, we found that following transfer to 30 °C, all autophagy-deficient (atg) mutants (atg2-1, 5-1, 7-2, and 10-1) of Arabidopsis thaliana tested displayed visibly impaired pollen development and anther dehiscence. HT-induced male sterility significantly increased in the atg mutants, but the degree of HT-induced obstacles did not change between the wild type (WT) and mutants from the seedling stage to the bolting stage. Cytological analyses showed that 30 °C promoted autophagy and autolysosome formation in both anther wall cells and microspores in developing anthers of WT, but the atg5-1 mutant did not show completion of tapetum degeneration and microspore maturation. HT upregulated hydrogen peroxide and dehydroascorbate reductase 1 production in both WT and atg5-1 anthers, but the basal levels were already higher in the mutant. HT repressed expression of UNDEAD and its regulator MYB80, which are required for tapetal programmed cell death (PCD) for proper pollen development. Taken together, our results suggest that autophagy functions in tapetum degeneration and pollen development during HT-caused tapetal PCD abortion.HighlightsIn Arabidopsis, autophagy is not essential for completion of the life cycle under normal temperatures.High temperature (HT) stress induces autophagy in developing anther wall cells and microspores.Autophagy deficient atg mutants become almost completely male-sterile at moderate HT.Autophagy plays a role in tapetum degeneration and pollen development during HT-caused abortion of tapetal program cell death.

scholarly journals High-Temperature Requirement A 1 Causes Photoreceptor Cell Death in Zebrafish Disease Models

2018 ◽  
Vol 188 (12) ◽  
pp. 2729-2744 ◽  
Author(s):  
Yoshihito Oura ◽  
Machiko Nakamura ◽  
Tohru Takigawa ◽  
Yoko Fukushima ◽  
Taku Wakabayashi ◽  
...  
Keyword(s):  
Cell Death ◽  
High Temperature ◽  
Photoreceptor Cell ◽  
Disease Models ◽  
Temperature Requirement

scholarly journals Modulation Effect in Oncothermia

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Oliver Szasz ◽  
Gabor Andocs ◽  
Nora Meggyeshazi
Keyword(s):  
Cell Death ◽  
High Temperature ◽  
Cell Membrane ◽  
Malignant Cell ◽  
High Energy ◽  
Modulation Effect ◽  
Periodic Patterns ◽  
Special Effects ◽  
Tumor Pathology ◽  
High Energy Absorption

Conventional hyperthermia is based on the local or systemic heating, which is measured by the realized temperature in the process. Oncothermia applies nanoheating, which means high energy absorption in the nanoscopic range of the malignant cell membrane selectively. This high temperature and its consequent stress create special effects: it evolves the possibility for chaperone proteins to be expressed on the outer membrane by softening the membrane and starts various excitations for programmed cell death of the targeted malignant cell. The process needs special delivery of the energy which selects as desired. A strict 13.56 MHz sinusoidal carrier frequency is amplitude modulated by time-fractal signals. The modulation is far from any sinus or other periodic patterns; it is a 1/f spectrum having definite templates for its construction. In some personalized cases, a definite template is used for the fractal pattern, which is copied from the actual character of the tumor pathology or any other specialty of the target.

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