Integrated genomics, physiology and breeding approaches for improving nitrogen use efficiency in potato: translating knowledge from other crops

2018 ◽  
Vol 45 (6) ◽  
pp. 587 ◽  
Author(s):  
Jagesh K. Tiwari ◽  
Darren Plett ◽  
Trevor Garnett ◽  
Swarup K. Chakrabarti ◽  
Rajesh K. Singh
Keyword(s):  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Production Costs ◽  
Environmental Damage ◽  
Nitrogen Use ◽  
N Metabolism ◽  
Use Efficiency ◽  
High Production ◽  
Food And Nutritional Security ◽  
Integrated Genomics

Potato plays a key role in global food and nutritional security. Potato is an N fertiliser-responsive crop, producing high tuber yields. However, excessive use of N can result in environmental damage and high production costs, hence improving nitrogen use efficiency (NUE) of potato plants is one of the sustainable options to address these issues and increase yield. Advanced efforts have been undertaken to improve NUE in other plants like Arabidopsis, rice, wheat and maize through molecular and physiological approaches. Conversely, in potato, NUE studies have predominantly focussed on agronomy or soil management, except for a few researchers who have measured gene expression and proteins relevant to N uptake or metabolism. The focus of this review is to adapt knowledge gained from other plants to inform investigation of N metabolism and associated traits in potato with the aim of improving potato NUE using integrated genomics, physiology and breeding methods.

scholarly journals Advances in Understanding the Molecular Mechanisms and Potential Genetic Improvement for Nitrogen Use Efficiency in Barley

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 662 ◽  
Author(s):  
Sakura D. Karunarathne ◽  
Yong Han ◽  
Xiao-Qi Zhang ◽  
Chengdao Li
Keyword(s):  
Nitrogen Use Efficiency ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Management Practices ◽  
N Uptake ◽  
Production Costs ◽  
Negative Consequences ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
The Impact

Nitrogen (N) fertilization plays an important role in crop production; however, excessive and inefficient use of N fertilizer is a global issue that incurs high production costs, pollutes the environment and increases the emission of greenhouse gases. To overcome these negative consequences, improving nitrogen use efficiency (NUE) would be a key factor for profitable crop production either by increasing yield or reducing fertilizer cost. In contrast to soil and crop management practices, understanding the molecular mechanisms in NUE and developing new varieties with improved NUE is more environmentally and economically friendly. In this review, we highlight the recent progress in understanding and improving nitrogen use efficiency in barley, with perspectives on the impact of N on plant morphology and agronomic performance, NUE and its components such as N uptake and utilization, QTLs and candidate genes controlling NUE, and new strategies for NUE improvement.

338 Nitrogen Use Efficiency Is Proportionally More Improved When Young Bulls Are Ranked on Residual Body Gain Rather Than on Residual Feed Intake

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 187-188
Author(s):  
Pablo Guarnido Lopez ◽  
Isabelle Ortigues Marty ◽  
Cantalapiedra-Hijar Gonzalo
Keyword(s):  
Nitrogen Use Efficiency ◽  
Feed Intake ◽  
Linear Models ◽  
Residual Feed Intake ◽  
Residual Body ◽  
Nitrogen Use ◽  
N Metabolism ◽  
Use Efficiency ◽  
Δ 15N ◽  
Positive Correlations

Abstract Animals with superior feed efficiency (FE) may also have an improved nitrogen use efficiency (NUE), which would be beneficial to economic profitability while reducing environmental impacts. When genetically selecting animals on FE, it is preferable to use residual traits [e.g. residual feed intake (RFI) or residual body gain (RG)] rather than ratios because of their predictable genetic outcomes. We studied the relationship of RFI and RG with NUE, estimated from the validated 15N abundance in plasma, across two contrasted diets based on corn or grass silages. We evaluated FE of 588 (half by diet) Charolais bulls (545 ± 57 kg BW) from 12 experimental cohorts (different farms and periods) over 200 days. Before the end of the FE test, plasma was sampled and analyzed for δ 15N. NUE was related to FE through simple-linear models with variables previously corrected for the cohort and diet effects. The models’ slopes were standardized according to FE deviation in order to compare the response of NUE to FE between indices. Higher NUE was related to higher FE (P < 0.001), showing positive correlations with RG (r=-0.40) and negative with RFI (r=0.29). However, the standardized slope of NUE to RG was significantly higher (+28%; P < 0.05) than that of NUE to RFI. This stronger NUE relation to RG compared to RFI could reflect a higher potential of RG animals to deposit N as compared to a more conservational N metabolism in RFI individuals. Regarding diets, and despite the correction of NUE and FE for this effect, the slopes of NUE to FE were numerically (P > 0.05) higher (-16% and +36%; for RG and RFI) in corn-based diets, which agrees with superior NUE observed in corn-vs-grass diets. Results suggested that superior RG animals may present proportionally higher NUE than superior RFI animals, with even better results in corn-vs-grass diets.

Identification of regulatory genes to improve nitrogen use efficiency

2014 ◽  
Vol 94 (6) ◽  
pp. 1009-1012 ◽  
Author(s):  
David R. Guevara ◽  
Yong-Mei Bi ◽  
Steven J. Rothstein
Keyword(s):  
Amino Acids ◽  
Transgenic Plants ◽  
Nitrogen Use Efficiency ◽  
Complex Traits ◽  
Regulatory Genes ◽  
Yield Potential ◽  
Environmental Damage ◽  
Nitrogen Use ◽  
Rice Plants ◽  
Use Efficiency

Guevara, D. R., Bi, Y.-M. and Rothstein, S. J. 2014. Identification of regulatory genes to improve nitrogen use efficiency. Can. J. Plant Sci. 94: 1009–1012. Crop production on soils containing sub-optimal levels of nitrogen (N) severely compromises yield potential. The development of crop varieties displaying high N use efficiency (NUE) is necessary in order to optimize N fertilizer use, and reduce the environmental damage caused by the current excessive application of N in agricultural areas. Genome-wide microarray analysis of rice plants grown under N-limiting environments was performed to identify NUE candidate genes. An early nodulin gene, OsENOD93-1, was strongly up-regulated during plant growth under low N. A constitutive Ubiquitin promoter was used to drive the expression of the OsENOD93-1 gene in transgenic plants to determine the importance of OsENOD93-1 for rice NUE. Transgenic rice plants over-expressing the OsENOD93-1 gene achieved ∼23% and 16% more yield and biomass, respectively, compared with wild-type plants when grown under N-limitation conditions. OsENOD93-1-OX transgenic plants accumulated a higher amount of total amino acids in the roots and xylem sap under N stress, suggesting that OsENOD93-1 plays a role in the transportation of amino acids. Taken together, we demonstrate that an effective way to identify NUE gene candidates involves both transcriptional profiling coupled with a transgenic validation approach to improve complex traits such as NUE in important crops.

scholarly journals Nitrogen Use Efficiency of Local and National Aromatic Rice Varieties in Indonesia

2018 ◽  
Vol 5 (3) ◽  
pp. 79-88
Author(s):  
Chairunnisak Chairunnisak ◽  
Sugiyanta Sugiyanta ◽  
Edi Santosa
Keyword(s):  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Aromatic Rice ◽  
Rice Varieties ◽  
Nitrogen Use ◽  
N Content ◽  
Low Input ◽  
Plot Yield ◽  
Use Efficiency ◽  
Sampling Plot

Nitrogen use efficiency (NUE) is a necessitate in order to enhance sustainable rice farming in Indonesia. Thus, objective of present research was to evaluate NUE of local and national Indonesian superior aromatic rice treated with different levels of nitrogen fertilizer (N). Planting plot was arranged using five levels of N as the main plot, i.e; 0, 45, 90, 135 and 180 kg ha-1; and two rice varieties as subplot, i.e: Sigupai Abdya (local) and Inpari 23 Bantul (national). The results showed application 180 kg N ha-1 to Sigupai  Abdya significantly increased the plant height. However, it also postponed the flowering time. Inpari 23 Bantul treated with 180 kg N ha-1 produced the highest number of tillers. Combination of 90 kg N ha-1 with Sigupai Abdya variety significantly reduced the number of empty grains. Sigupai Abdya variety has a higher number of grains per panicle and sampling plot yield than Inpari 23 Bantul, and  dosage 90 kg N ha-1 increases grain yield per clump also sampling plot yield significantly. Nitrogen at 180 kg N ha-1 made Sigupai Abdya variety has high N content and absorption N in primordia phase, and the Inpari 23 Bantul variety had grain with high N content. Nitrogen at 90 kg ha-1 caused Sigupai Abdya variety at primordia phase had NUE higher than Inpari 23 Bantul. This study showed that local variety Sigupai Abdya is suitable for development as rice with low input NKeywords: Aceh aromatic, low input, nitrogen dose, N uptake, Oryza sativa L. 

scholarly journals Penggunaan Azotobacter dan Kompos Kulit Buah Kakao Terhadap Pertumbuhan dan Efisiensi Penggunaan Nitrogen Pada Pembibitan Utama Kelapa Sawit

2021 ◽  
Vol 23 (1) ◽  
pp. 50
Author(s):  
Wilda Lumban Tobing ◽  
Mariani Sembiring
Keyword(s):  
Nitrogen Use Efficiency ◽  
Oil Palm ◽  
N Uptake ◽  
Dry Weight ◽  
Research Area ◽  
College Of Agriculture ◽  
Nitrogen Use ◽  
Fruit Skin ◽  
Use Efficiency ◽  
The University

<p>The expansion of oil palm plantations continues to increase so that it requires quality seed. Nurseries need to be done before moving to the field. The use of Azotobacter and cocoa fruit skin compost is one way to increase the  growth and nitrogen use efficiency in oil palm in main nursery. This research was aimed to know the growth and nitrogen use efficiency of palm oil in main nursery. This research was conducted on the research area of the Agricultural Agribusiness College of Agriculture Practices (STIPAP) Medan and the Laboratory of Balai Pengkajian Teknologi Pertanian (BPTP) of North Sumatra and the Agricultural Laboratory of the University of North Sumatera from February until Juli 2013. The method used was Randomized Group Design factorial with 3 replications and followed by Duncan test at α=5%. The first factor is Azotobacter including without giving Azotobacter (A0), 20 ml/polybag (A1) and 40 ml/polybag (A2). The second factor is cocoa fruit skin compost, which consists of 3 of them, namely without compost (K0), 125 g/polybag (K1), and 250 g/polybag (K2). The research parameters were plant dry weight (g), nitrogen uptake (mg) and N use efficiency (EPN). The results showed that the use of Azotobacter and cocoa fruit skin compost were able to significantly increasing dry weight of plant and N uptake of oil palm seeds and gave the highest EPN value of 12.93.  </p>

scholarly journals Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Muhammad Anas ◽  
Fen Liao ◽  
Krishan K. Verma ◽  
Muhammad Aqeel Sarwar ◽  
Aamir Mahmood ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrogen Uptake ◽  
Nitrogen Use Efficiency ◽  
Molecular Form ◽  
Plant Population ◽  
Production Costs ◽  
Utilization Efficiency ◽  
Optimal Dosage ◽  
Nitrogen Use ◽  
Use Efficiency

Abstract Nitrogen is the main limiting nutrient after carbon, hydrogen and oxygen for photosynthetic process, phyto-hormonal, proteomic changes and growth-development of plants to complete its lifecycle. Excessive and inefficient use of N fertilizer results in enhanced crop production costs and atmospheric pollution. Atmospheric nitrogen (71%) in the molecular form is not available for the plants. For world’s sustainable food production and atmospheric benefits, there is an urgent need to up-grade nitrogen use efficiency in agricultural farming system. The nitrogen use efficiency is the product of nitrogen uptake efficiency and nitrogen utilization efficiency, it varies from 30.2 to 53.2%. Nitrogen losses are too high, due to excess amount, low plant population, poor application methods etc., which can go up to 70% of total available nitrogen. These losses can be minimized up to 15–30% by adopting improved agronomic approaches such as optimal dosage of nitrogen, application of N by using canopy sensors, maintaining plant population, drip fertigation and legume based intercropping. A few transgenic studies have shown improvement in nitrogen uptake and even increase in biomass. Nitrate reductase, nitrite reductase, glutamine synthetase, glutamine oxoglutarate aminotransferase and asparagine synthetase enzyme have a great role in nitrogen metabolism. However, further studies on carbon–nitrogen metabolism and molecular changes at omic levels are required by using “whole genome sequencing technology” to improve nitrogen use efficiency. This review focus on nitrogen use efficiency that is the major concern of modern days to save economic resources without sacrificing farm yield as well as safety of global environment, i.e. greenhouse gas emissions, ammonium volatilization and nitrate leaching.

scholarly journals N-(n-Butyl) Thiophosphoric Triamide (NBPT)-Coated Urea (NCU) Improved Maize Growth and Nitrogen Use Efficiency (NUE) in Highly Weathered Tropical Soil

2020 ◽  
Vol 12 (21) ◽  
pp. 8780
Author(s):  
Muhammad Muhaymin Mohd Zuki ◽  
Noraini Md. Jaafar ◽  
Siti Zaharah Sakimin ◽  
Mohd Khanif Yusop
Keyword(s):  
Nitrogen Use Efficiency ◽  
Plant Uptake ◽  
N Uptake ◽  
Growth Yield ◽  
Dry Weight ◽  
Tropical Soil ◽  
Nitrogen Use ◽  
Weight Yield ◽  
Coated Urea ◽  
Use Efficiency

Nitrogen (N) fertilizer is commonly used to supply sufficient N for plant uptake, for which urea is one of the highly preferred synthetic N fertilizers due to its high N content. Unfortunately, N provided by urea is rapidly lost upon urea application to soils through ammonia volatilization, leaching, and denitrification. Thus, treatment of urea with urease inhibitor (N-(n-Butyl) Thiophosphoric Triamide (NBPT)) is among the solutions to slow down urea hydrolysis, therefore reducing loss of NH3 and saving N available for plant uptake and growth. A field study was carried out to evaluate the effects of NBPT-coated urea (NCU) at varying rates on growth, yield, and nitrogen use efficiency (NUE) of maize in tropical soil. The experiment was conducted at Field 15, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, and maize (Zea mays var. Thai Super Sweet) was used as the test crop. The results showed that all maize grown in soils applied with urea coated with NBPT (NCU) (T2, T3, T4, and T5) had significantly (P ≤ 0.05) higher chlorophyll content compared to the control (T0 and T1). The surface leaf area of maize grown in NCU-treated soils at 120 kg N h−1 (T3) was recorded as the highest. NCU at and 96 kg N ha−1 (T3 and T4) were relatively effective in increasing maize plant dry weight, yield, and N uptake. Improvement of NUE by 45% over urea was recorded in the treatment of NCU at 96 kg N ha−1. NBPT-coated urea (NCU) at 96 kg N ha−1 had potential to increase the growth, yield, nitrogen uptake, and NUE of maize by increasing the availability of N for plant growth and development.

Nitrogen use efficiency, economic return and yield performance of Pigeonpea [Cajanus cajan (L.) Millsp.] as influenced by nipping and fertility levels

2019 ◽  
Author(s):  
A.K. Dhaka ◽  
Satish Kumar ◽  
Bhagat Singh ◽  
Karmal Singh ◽  
Amit Kumar ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Utilization Efficiency ◽  
N Balance ◽  
Flower Initiation ◽  
Recovery Efficiency ◽  
Nitrogen Use ◽  
Total N ◽  
Yield Attributes ◽  
Use Efficiency

An experiment was conducted to study nitrogen use efficiency in pigeonpea at Research farm, CCS Haryana Agricultural University, Hisar, India having three nipping treatments (no nipping, nipping at just start of branching and nipping at flower initiation) and five fertility levels (control, 20 kg N + 40 kg P2O5/ha, 30 kg N + 40 kg P2O5 /ha, 40 kg N + 40 kg P2O5/ha and 20 kg N + 40 kg P2O5/ha + foliar spray of 2% N immediately after nipping) replicated thrice in split plot design during growing seasons of 2016 and 2017. Nipping at start of branching reduced the plant height, while increased primary and secondary branches, pods/plant and yield over no nipping. Significantly higher total N uptake, protein content, net return, B: C, agronomical NUE, physiologic NUE, agro-physiologic NUE, apparent recovery efficiency, utilization efficiency of N and partial N balance were improved with nipping at start of branching. Among fertility levels, 40 kg N + 40 kg P2O5 / ha recorded significantly higher yield attributes with 39.7 per cent higher seed yield over control. Significantly higher agronomic NUE, physiologic NUE, agro-physiological NUE, apparent recovery efficiency, utilization efficiency of N, partial N balance and NER were recorded with 20 kg/ha as compared to higher nitrogen doses.

scholarly journals Genetic Engineering and Genome Editing for Improving Nitrogen Use Efficiency in Plants

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3303
Author(s):  
Vadim G. Lebedev ◽  
Anna A. Popova ◽  
Konstantin A. Shestibratov
Keyword(s):  
Genome Editing ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Availability ◽  
Global Climate ◽  
Biomass Accumulation ◽  
Limiting Factors ◽  
Unintended Effects ◽  
Nitrogen Use ◽  
N Metabolism ◽  
Use Efficiency

Low nitrogen availability is one of the main limiting factors for plant growth and development, and high doses of N fertilizers are necessary to achieve high yields in agriculture. However, most N is not used by plants and pollutes the environment. This situation can be improved by enhancing the nitrogen use efficiency (NUE) in plants. NUE is a complex trait driven by multiple interactions between genetic and environmental factors, and its improvement requires a fundamental understanding of the key steps in plant N metabolism—uptake, assimilation, and remobilization. This review summarizes two decades of research into bioengineering modification of N metabolism to increase the biomass accumulation and yield in crops. The expression of structural and regulatory genes was most often altered using overexpression strategies, although RNAi and genome editing techniques were also used. Particular attention was paid to woody plants, which have great economic importance, play a crucial role in the ecosystems and have fundamental differences from herbaceous species. The review also considers the issue of unintended effects of transgenic plants with modified N metabolism, e.g., early flowering—a research topic which is currently receiving little attention. The future prospects of improving NUE in crops, essential for the development of sustainable agriculture, using various approaches and in the context of global climate change, are discussed.

scholarly journals Review of Active Optical Sensors for Improving Winter Wheat Nitrogen Use Efficiency

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1157
Author(s):  
Lawrence Aula ◽  
Peter Omara ◽  
Eva Nambi ◽  
Fikayo B. Oyebiyi ◽  
William R. Raun
Keyword(s):  
Winter Wheat ◽  
Grain Yield ◽  
Optical Sensors ◽  
Nitrogen Use Efficiency ◽  
Optical Sensor ◽  
Management Practices ◽  
N Uptake ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Management

Improvement of nitrogen use efficiency (NUE) via active optical sensors has gained attention in recent decades, with the focus of optimizing nitrogen (N) input while simultaneously sustaining crop yields. To the authors’ knowledge, a comprehensive review of the literature on how optical sensors have impacted winter wheat (Triticum aestivum L.) NUE and grain yield has not yet been performed. This work reviewed and documented the extent to which the use of optical sensors has impacted winter wheat NUE and yield. Two N management approaches were evaluated; optical sensor and conventional methods. The study included 26 peer-reviewed articles with data on NUE and grain yield. In articles without NUE values but in which grain N was included, the difference method was employed to compute NUE based on grain N uptake. Using optical sensors resulted in an average NUE of 42% (±2.8% standard error). This approach improved NUE by approximately 10.4% (±2.3%) when compared to the conventional method. Grain yield was similar for both approaches of N management. Optical sensors could save as much as 53 (±16) kg N ha−1. This gain alone may not be adequate for increased adoption, and further refinement of the optical sensor robustness, possibly by including weather variables alongside sound agronomic management practices, may be necessary.

Sign in / Sign up

Export Citation Format

Share Document