The half-life of the cytochrome bf complex in leaves of pea plants after transfer from moderately-high growth light to low light

2017 ◽  
Vol 44 (3) ◽  
pp. 351 ◽  
Author(s):  
Hui Zhu ◽  
Ling-Da Zeng ◽  
Xiao-Ping Yi ◽  
Chang-Lian Peng ◽  
Wang-Feng Zhang ◽  
...  
Keyword(s):  
Half Life ◽  
Time Course ◽  
Photosynthetic Capacity ◽  
High Growth ◽  
Limiting Factor ◽  
Low Light ◽  
High Photosynthetic Capacity ◽  
Growth Irradiance ◽  
The Difference ◽  
Time Courses

The content of cytochrome (cyt) bf complex is the main rate-limiting factor that determines light- and CO2-saturated photosynthetic capacity. A study of the half-life of the cyt f content in leaves was conducted whereby Pisum sativum L. plants, grown in moderately high light (HL), were transferred to low light (LL). The cyt f content in fully-expanded leaves decreased steadily over the 2 weeks after the HL-to-LL transfer, whereas control leaves in HL retained their high contents. The difference between the time courses of HL-to-LL plants and control HL plants represents the time course of loss of cyt f content, with a half-life of 1.7 days, which is >3-fold shorter than that reported for tobacco leaves at constant growth irradiance using an RNA interference approach (Hojka et al. 2014). After transfer to LL (16 h photoperiod), pea plants were re-exposed to HL for 0, 1.5 h or 5 h during the otherwise LL photoperiod, but the cyt f content of fully-expanded leaves declined practically at the same rate regardless of whether HL was re-introduced for 0, 1.5 h or 5 h during each 16 h LL photoperiod. It appears that fully-expanded leaves, having matured under HL, were unable to increase their cyt f content when re-introduced to HL. These findings are relevant to any attempts to maintain a high photosynthetic capacity when the growth irradiance is temporarily decreased by shading or overcast weather.

scholarly journals Time Course of Perceived Visual Distortion and Axial Length Growth in Myopic Children Undergoing Orthokeratology

2021 ◽  
Vol 15 ◽  
Author(s):  
Guihua Liu ◽  
Yiyuan Wu ◽  
Hua Bi ◽  
Biying Wang ◽  
Tianpu Gu ◽  
...  
Keyword(s):  
Axial Length ◽  
Visual Function ◽  
Time Course ◽  
Optical Quality ◽  
Orientation Discrimination ◽  
Length Growth ◽  
The Difference ◽  
Time Courses ◽  
Transient Elevation ◽  
Lens Wear

Purpose: To establish the time course of the subjective visual function changes during the first month of orthokeratology treatment in myopic children, and to investigate how the time course variations are associated with the objective optical quality changes and the axial length growth (ALG) after 1 year of treatment.Methods: A total of 58 myopic children aged from 8 to 16 years participated in this self-controlled prospective study. All subjects were fitted with designed spherical four-zone orthokeratology lenses. Subjective visual function was evaluated with orientation discrimination threshold (ODT), and objective optical quality was quantified with the high-order aberration root-mean-square (HOA-RMS) and the changing speed of HOA. The measurements were done before the lens fitting and 1 day, 1-, 2-, and 4-weeks after lens wear. Axial length was obtained at baseline and 1-year follow-up, and ALG was defined as the difference. One-way ANOVA was conducted to compare the difference for statistical analysis.Results: After lens fitting, the ODT time courses peaked on day 1 in 28 children, 1 week in 15 children, 2 weeks in 11 children, and 4 weeks in 4 children. In contrast, the HOA-RMS steadily rose during the first month, and the changing speed of HOA was only transiently elevated on day 1 after the initial lens wear. The ALG was 0.12 ± 0.20 mm in subjects whose ODT peaked at day 1, 0.08 ± 0.09 mm in subjects whose ODT peaked on 1-week, and 0.12 ± 0.15 mm in subjects whose ODT peaked on 2-week or later. There was no difference in axial growth among the subjects whose ODT peaked at different days (P = 0.734).Conclusion: While half ODT time course resembled the changing speed of HOA with a transient elevation on day 1, about a quarter of the ODT time course resemble the steadily rising of HOA-RMS, and the rest was located in the middle. The ALGs in children with different types of ODT time courses were similar.

scholarly journals A phenomenological model of the time course of maximal voluntary isometric contraction force for optimization of complex loading schemes

2018 ◽  
Author(s):  
Johannes L. Herold ◽  
Christian Kirches ◽  
Johannes P. Schlöder
Keyword(s):  
Isometric Contraction ◽  
Time Course ◽  
Complex Loading ◽  
Equation Model ◽  
Limiting Factor ◽  
Published Data ◽  
Ordinary Differential Equation Model ◽  
Differential Equation Model ◽  
Maximal Voluntary Isometric Contraction ◽  
Time Courses

AbstractWe construct a simple and predictive ordinary differential equation model to describe the time course of maximal voluntary isometric contraction (MVIC) force during voluntary isometric contractions and at rest. These time courses are of particular interest whenever force capacities are a limiting factor, e.g. during heavy manual work or resistance training (RT) sessions. Our model is able to describe MVIC force under complex loading schemes and is validated with a comprehensive set of published data from the elbow flexors. We use the calibrated model to analyze fatigue and recovery patterns observed in the literature. Due to the model’s structure, it can be efficiently employed to optimize complex loading schemes. We demonstrate this by computing a work-rest schedule that minimizes fatigue and an optimal isometric RT session as examples.

scholarly journals Effect of Nitrogen and Potassium Fertilization Doses on Elephant-Grass Genotypes for Energy Purposes

2018 ◽  
Vol 10 (10) ◽  
pp. 446
Author(s):  
Wanessa Francesconi Stida ◽  
Brunno de Oliveira Almeida ◽  
Rogério Figueiredo Daher ◽  
Cássia Roberta de Oliveira Moraes ◽  
Ana Kesia Faria Vidal ◽  
...  
Keyword(s):  
Alternative Energy ◽  
Photosynthetic Capacity ◽  
Agronomic Traits ◽  
High Growth ◽  
Elephant Grass ◽  
Alternative Source ◽  
Potassium Fertilization ◽  
High Photosynthetic Capacity ◽  
High Production ◽  
Positive Effect

In view of the current global energy landscape, to develop alternative energy mechanisms to the oil has become essential. For that, biomass as well as the use of elephant-grass present themselves as attractive choices for energy purposes. That culture has a high growth prospect, as it contains characteristics such as high production, biomass quality, and high photosynthetic capacity. The purpose of this work was to assess the response of eight elephant-grass genotypes to nitrogen and potassium fertilization from the evaluation of morpho-agronomic traits. It was used a randomized block experimental design with three replications in the factorial scheme within a subdivided plot composed of principal factor (plots): genotypes and secondary components (subplots): potassium (2 levels) × nitrogen (3 levels)—200 × 400, 200 × 1000, 200 × 1600, 500 × 400, 500 × 1000, and 500 × 1600 kg ha-1. There was an adjustment of first degree linear model of the regression for all traits in at least one genotype. For DMP, the Capim Cana D’África, CPAC, and IJ 7139 genotypes indicated an inversely proportional response to the increasing of N in the fertilization. The response according to the N increasing in the fertilization was directly proportional for the CPAC genotype in relation to the NP, and for the Cana D’África, CPAC, and IJ 7139 genotypes in relation to the ALT. For SD, the CPAC and Vruckwona genotypes showed a positive effect on the increasing doses of N, and the IJ 7139 genotype, a negative correlation. The results are quite promising and ensure the use of the eight elephant-grass genotypes as an alternative source for biomass production.

Destruction of Actin Filaments by Osmium Tetroxide

1977 ◽  
Vol 35 ◽  
pp. 466-467
Author(s):  
P. Maupin-Szamier ◽  
T. D. Pollard
Keyword(s):  
Actin Filaments ◽  
Time Course ◽  
Osmium Tetroxide ◽  
Rabbit Muscle ◽  
Muscle Actin ◽  
Sodium Phosphate Buffer ◽  
Transmission Electron ◽  
The Difference ◽  
Rates Of Decay ◽  
Short Time

We have studied the destruction of rabbit muscle actin filaments by osmium tetroxide (OSO4) to develop methods which will preserve the structure of actin filaments during preparation for transmission electron microscopy.Negatively stained F-actin, which appears as smooth, gently curved filaments in control samples (Fig. 1a), acquire an angular, distorted profile and break into progressively shorter pieces after exposure to OSO4 (Fig. 1b,c). We followed the time course of the reaction with viscometry since it is a simple, quantitative method to assess filament integrity. The difference in rates of decay in viscosity of polymerized actin solutions after the addition of four concentrations of OSO4 is illustrated in Fig. 2. Viscometry indicated that the rate of actin filament destruction is also dependent upon temperature, buffer type, buffer concentration, and pH, and requires the continued presence of OSO4. The conditions most favorable to filament preservation are fixation in a low concentration of OSO4 for a short time at 0°C in 100mM sodium phosphate buffer, pH 6.0.

scholarly journals Pseudo-streaming potentials in Necturus gallbladder epithelium. II. The mechanism is a junctional diffusion potential.

1992 ◽  
Vol 99 (3) ◽  
pp. 317-338 ◽  
Author(s):  
L Reuss ◽  
B Simon ◽  
C U Cotton
Keyword(s):  
Time Course ◽  
Bathing Solution ◽  
Intercellular Spaces ◽  
Similar Time ◽  
Streaming Potentials ◽  
Osmotic Water ◽  
Lateral Intercellular Spaces ◽  
Transepithelial Voltage ◽  
Time Courses ◽  
Good Agreement

The mechanisms of apparent streaming potentials elicited across Necturus gallbladder epithelium by addition or removal of sucrose from the apical bathing solution were studied by assessing the time courses of: (a) the change in transepithelial voltage (Vms). (b) the change in osmolality at the cell surface (estimated with a tetrabutylammonium [TBA+]-selective microelectrode, using TBA+ as a tracer for sucrose), and (c) the change in cell impermeant solute concentration ([TMA+]i, measured with an intracellular double-barrel TMA(+)-selective microelectrode after loading the cells with TMA+ by transient permeabilization with nystatin). For both sucrose addition and removal, the time courses of Vms were the same as the time courses of the voltage signals produced by [TMA+]i, while the time courses of the voltage signals produced by [TBA+]o were much faster. These results suggest that the apparent streaming potentials are caused by changes of [NaCl] in the lateral intercellular spaces, whose time course reflects the changes in cell water volume (and osmolality) elicited by the alterations in apical solution osmolality. Changes in cell osmolality are slow relative to those of the apical solution osmolality, whereas lateral space osmolality follows cell osmolality rapidly, due to the large surface area of lateral membranes and the small volume of the spaces. Analysis of a simple mathematical model of the epithelium yields an apical membrane Lp in good agreement with previous measurements and suggests that elevations of the apical solution osmolality elicit rapid reductions in junctional ionic selectivity, also in good agreement with experimental determinations. Elevations in apical solution [NaCl] cause biphasic transepithelial voltage changes: a rapid negative Vms change of similar time course to that of a Na+/TBA+ bi-ionic potential and a slow positive Vms change of similar time course to that of the sucrose-induced apparent streaming potential. We conclude that the Vms changes elicited by addition of impermeant solute to the apical bathing solution are pseudo-streaming potentials, i.e., junctional diffusion potentials caused by salt concentration changes in the lateral intercellular spaces secondary to osmotic water flow from the cells to the apical bathing solution and from the lateral intercellular spaces to the cells. Our results do not support the notion of junctional solute-solvent coupling during transepithelial osmotic water flow.

A laboratory study of vertical migration

1955 ◽  
Vol 144 (916) ◽  
pp. 329-354 ◽  
Keyword(s):  
Light Intensity ◽  
Vertical Migration ◽  
Time Course ◽  
Tap Water ◽  
Low Light ◽  
Overhead Light ◽  
Light Intensities ◽  
Orientation Response ◽  
Reduced Light ◽  
Characteristic Maximum

In a tank filled with a suspension of indian ink in tap water, a population of Daphnia magna will undergo a complete cycle of vertical migration when an overhead light source is cycli­cally varied in intensity. A ‘dawn rise’ to the surface at low intensity is followed by the descent of the animals to a characteristic maximum depth. The animals rise to the surface again as the light decreases, and finally show a typical midnight sinking. The light intensities at the level of the animals in this experiment are of the same order as those which have been reported in field observations; the time course of the movement also repeats the natural conditions in the field. The process is independent of the duration of the cycle and is related only to the variation in overhead light intensity. At low light intensity the movement of the animal is determined solely by positive photo-kinesis; the dawn rise is a manifestation of this, and is independent of the direction of the light. At high light intensities there is an orientation response which is superimposed upon an alternating positive (photokinetic) phase and a negative phase during which movement is inhibited. The fully oriented animal shows a special type of positive and negative phototaxis, moving towards the light at reduced light intensities and away from it when the light intensity is increased. In this condition it follows a zone of optimum light intensity with some exactness. Experiments show that an animal in this fully oriented condition will respond to the slow changes of intensity characteristic of the diurnal cycle, while being little affected by tran­sient changes of considerable magnitude.

scholarly journals Sodium and calcium currents in dispersed mammalian septal neurons.

1991 ◽  
Vol 97 (2) ◽  
pp. 303-320 ◽  
Author(s):  
A Castellano ◽  
J López-Barneo
Keyword(s):  
Time Constant ◽  
Time Course ◽  
Calcium Currents ◽  
Closing Time ◽  
Patch Clamp Technique ◽  
Average Value ◽  
Time To Peak ◽  
Time Courses ◽  
Fast Activation ◽  
Septal Neurons

Voltage-gated Na+ and Ca2+ conductances of freshly dissociated septal neurons were studied in the whole-cell configuration of the patch-clamp technique. All cells exhibited a large Na+ current with characteristic fast activation and inactivation time courses. Half-time to peak current at -20 mV was 0.44 +/- 0.18 ms and maximal activation of Na+ conductance occurred at 0 mV or more positive membrane potentials. The average value was 91 +/- 32 nS (approximately 11 mS cm-2). At all membrane voltages inactivation was well fitted by a single exponential that had a time constant of 0.44 +/- 0.09 ms at 0 mV. Recovery from inactivation was complete in approximately 900 ms at -80 mV but in only 50 ms at -120 mV. The decay of Na+ tail currents had a single time constant that at -80 mV was faster than 100 microseconds. Depolarization of septal neurons also elicited a Ca2+ current that peaked in approximately 6-8 ms. Maximal peak Ca2+ current was obtained at 20 mV, and with 10 mM external Ca2+ the amplitude was 0.35 +/- 0.22 nA. During a maintained depolarization this current partially inactivated in the course of 200-300 ms. The Ca2+ current was due to the activity of two types of conductances with different deactivation kinetics. At -80 mV the closing time constants of slow (SD) and fast (FD) deactivating channels were, respectively, 1.99 +/- 0.2 and 0.11 +/- 0.03 ms (25 degrees C). The two kinds of channels also differed in their activation voltage, inactivation time course, slope of the conductance-voltage curve, and resistance to intracellular dialysis. The proportion of SD and FD channels varied from cell to cell, which may explain the differential electrophysiological responses of intracellularly recorded septal neurons.

scholarly journals Mechanisms of autoantibody production in autoimmune MRL mice.

1980 ◽  
Vol 152 (5) ◽  
pp. 1302-1310 ◽  
Author(s):  
D S Pisetsky ◽  
G A McCarty ◽  
D V Peters
Keyword(s):  
Antibody Response ◽  
Time Course ◽  
Fundamental Difference ◽  
Autoantibody Production ◽  
Quantitative Expression ◽  
Autoantibody Response ◽  
Time Courses ◽  
Antibody Levels

The quantitative expression of anti-DNA and anti-Sm antibodies has been investigated in autoimmune MRL-lpr/lpr and MRL-+/+ mice. Anti-Sm antibodies were detected in sera from 21/23 lpr/lpr and 10/16 +/+ mice, with individual animals showing striking variation in the time-course and magnitude of this autoantibody response. The peak antibody levels of the responding animals of each substrain did not differ significantly. For anti-DNA antibody, a different pattern of responsiveness was observed. Individual animals of each substrain produced very similar responses in terms of the magnitude and time-course of serum anti-DNA antibody. The differences in the peak levels of the two substrains were highly significant, with lpr/lpr mice demonstrating a much greater anti-DNA antibody response than +/+ mice. In lpr/lpr mice tested for both autoantibody systems, serum anti-DNA and anti-Sm antibodies showed distinct time-courses. These studies indicate that anti-DNA and anti-Sm antibodies are expressed independently in MRL mice, with the expression of anti-DNA, but not anti-Sm antibody markedly influenced by the presence of the 1pr gene. A fundamental difference in the mechanisms involved in the generation of anti-DNA and anti-Sm antibodies is suggested by the quantitative pattern of the two responses.

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