Copper amine oxidase-catalysed hydrogen peroxide involves production of nitric oxide in darkness-induced stomatal closure in broad bean

2015 ◽  
Vol 42 (11) ◽  
pp. 1057 ◽  
Author(s):  
Ai-Xia Huang ◽  
Yong-Shun Wang ◽  
Xiao-Ping She ◽  
Juan Mu ◽  
Jin-Liang Zhao
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Amine Oxidase ◽  
Stomatal Closure ◽  
H2o2 Production ◽  
No Production ◽  
Copper Amine Oxidase ◽  
Irreversible Inhibitors ◽  
Important Intermediate ◽  
Copper Amine

Hydrogen peroxide is an important intermediate in darkness-induced stomatal closure. In the present work, we provide evidence that copper amine oxidase (CuAO) was involved in H2O2 production in darkness-induced stomatal closure in Vicia faba L. Darkness activated CuAO in intercellular washing fluid from leaves. Aminoguanidine (AG) and 2-bromoethylamine (BEA), which were both irreversible inhibitors of CuAO, significantly suppressed darkness-induced stomatal closure and H2O2 generation. The effects of AG and BEA were reversed only by H2O2 but not by other products of CuAO. These results indicate that CuAO participates in darkness-induced stomatal closure through its reaction product, H2O2. Furthermore, darkness-induced nitric oxide (NO) production and cytosolic alkalinisation were obviously inhibited by AG and BEA, and only H2O2, among the products of CuAO, could reverse the effects, implying that the CuAO-catalysed product H2O2 is required for NO production and cytosolic alkalinisation to a large extent in darkness-induced stomatal closure. In addition, butyric acid blocked but methylamine enhanced the ability of H2O2 to reverse the effect of BEA on NO production, suggesting that cytosolic alkalinisation is involved in CuAO-mediated NO generation in darkness-induced stomatal closure.

scholarly journals The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 183 ◽  
Author(s):  
Ilaria Fraudentali ◽  
Sandip A. Ghuge ◽  
Andrea Carucci ◽  
Paraskevi Tavladoraki ◽  
Riccardo Angelini ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Amine Oxidase ◽  
Stomatal Closure ◽  
H2o2 Production ◽  
Amine Oxidases ◽  
Wild Type ◽  
Copper Amine Oxidase ◽  
Copper Amine Oxidases ◽  
Copper Amine

Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAOδ (AtCuAOδ; At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAOδ is up-regulated by ABA and that two Atcuaoδ T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAOδ over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2O2 production compared to WT plants. Our data suggest that AtCuAOδ is involved in the H2O2 production related to ABA-induced stomatal closure.

The regulator of G-protein signalling protein mediates D-glucose-induced stomatal closure via triggering hydrogen peroxide and nitric oxide production in Arabidopsis

2018 ◽  
Vol 45 (5) ◽  
pp. 509 ◽  
Author(s):  
Shumei Hei ◽  
Zhifeng Liu ◽  
Aixia Huang ◽  
Xiaoping She
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
G Protein ◽  
Stomatal Closure ◽  
H2o2 Production ◽  
No Production ◽  
Α Subunit ◽  
G Protein Signalling ◽  
Guanine Nucleotide Binding ◽  
Nucleotide Binding Proteins

2-Deoxy-D-glucose, 3-O-methyl-D-glucose and D-mannose are all non-metabolisable D-glucose analogues. Among these, 2-deoxy-D-glucose and D-mannose are substrates for hexokinase (HXK). D-sorbitol and D-mannitol are reduced forms of D-glucose and are typically used as comparable osmotic solutes. Similar to 2-deoxy-D-glucose and D-mannose, D-glucose induced stomatal closure in Arabidopsis, whereas 3-O-methyl-D-glucose, D-sorbitol and D-mannitol did not. The data show that the effect of D-glucose on stomata is metabolism-independent, HXK-dependent and irrelevant to osmotic stress. Additionally, the D-glucose induced closure of stomata in wild-type Arabidopsis, but did not in rgs1-1 and rgs1-2 or gpa1-3 and gpa1-4 mutants, indicating that the regulator of G-protein signalling protein (RGS1) and heterotrimeric guanine nucleotide-binding proteins (G proteins)-α subunit (Gα) also mediate the stomatal closure triggered by D-glucose. Furthermore, the effects of D-glucose on hydrogen peroxide (H2O2) or nitric oxide (NO) production and stomatal closure were more significant in AtrbohD or Nia2-1 mutants than in AtrbohF and AtrbohD/F or Nia1-2 and Nia2-5/Nia1-2. The data indicate that H2O2 sourced from AtrbohF and NO generated by Nia1 are essential for D-glucose-mediated stomatal closure. D-glucose-induced H2O2 and NO production in guard cells were completely abolished in rgs1-1 and rgs1-2, which suggests that RGS1 stimulates H2O2 and NO production in D-glucose-induced stomatal closure. Collectively, our data reveal that both HXK and RGS1 are required for D-glucose-mediated stomatal closure. In this context, D-glucose can be sensed by its receptor RGS1, thereby inducing AtrbohF-dependent H2O2 production and Nia1-catalysed NO accumulation, which in turn stimulates stomatal closure.

scholarly journals Nitric Oxide and Hydrogen Peroxide Signaling in Extractive Shiraia Fermentation by Triton X-100 for Hypocrellin A Production

2020 ◽  
Vol 21 (3) ◽  
pp. 882
Author(s):  
Xin Ping Li ◽  
Yue Wang ◽  
Yan Jun Ma ◽  
Jian Wen Wang ◽  
Li Ping Zheng
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
H2o2 Production ◽  
No Production ◽  
Extractive Fermentation ◽  
Hypocrellin A ◽  
Cell Membrane Permeabilization ◽  
Rapid Generation ◽  
Triton X

Shiraia mycelial culture is a promising biotechnological alternative for the production of hypocrellin A (HA), a new photosensitizer for anticancer photodynamic therapy (PDT). The extractive fermentation of intracellular HA in the nonionic surfactant Triton X-100 (TX100) aqueous solution was studied in the present work. The addition of 25 g/L TX100 at 36 h of the fermentation not only enhanced HA exudation to the broth by 15.6-fold, but stimulated HA content in mycelia by 5.1-fold, leading to the higher production 206.2 mg/L, a 5.4-fold of the control on day 9. After the induced cell membrane permeabilization by TX100 addition, a rapid generation of nitric oxide (NO) and hydrogen peroxide (H2O2) was observed. The increase of NO level was suppressed by the scavenger vitamin C (VC) of reactive oxygen species (ROS), whereas the induced H2O2 production could not be prevented by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), suggesting that NO production may occur downstream of ROS in the extractive fermentation. Both NO and H2O2 were proved to be involved in the expressions of HA biosynthetic genes (Mono, PKS and Omef) and HA production. NO was found to be able to up-regulate the expression of transporter genes (MFS and ABC) for HA exudation. Our results indicated the integrated role of NO and ROS in the extractive fermentation and provided a practical biotechnological process for HA production.

scholarly journals A New Player in Jasmonate-Mediated Stomatal Closure: The Arabidopsis thaliana Copper Amine Oxidase β

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3399
Author(s):  
Ilaria Fraudentali ◽  
Chiara Pedalino ◽  
Paraskevi Tavladoraki ◽  
Riccardo Angelini ◽  
Alessandra Cona
Keyword(s):  
Arabidopsis Thaliana ◽  
Amine Oxidase ◽  
Stomatal Closure ◽  
Plant Defence ◽  
Specific Inhibitor ◽  
Guard Cells ◽  
H2o2 Production ◽  
Specific Expression ◽  
Adverse Environmental Conditions ◽  
Copper Amine

Plant defence responses to adverse environmental conditions include different stress signalling, allowing plant acclimation and survival. Among these responses one of the most common, immediate, and effective is the modulation of the stomatal aperture, which integrates different transduction pathways involving hydrogen peroxide (H2O2), calcium (Ca2+), nitric oxide (NO), phytohormones and other signalling components. The Arabidopsis thaliana copper amine oxidases β (AtCuAOβ) encodes an apoplastic CuAO expressed in guard cells and root protoxylem tissues which oxidizes polyamines to aminoaldehydes with the production of H2O2 and ammonia. Here, its role in stomatal closure, signalled by the wound-associated phytohormone methyl-jasmonate (MeJA) was explored by pharmacological and genetic approaches. Obtained data show that AtCuAOβ tissue-specific expression is induced by MeJA, especially in stomata guard cells. Interestingly, two Atcuaoβ T-DNA insertional mutants are unresponsive to this hormone, showing a compromised MeJA-mediated stomatal closure compared to the wild-type (WT) plants. Coherently, Atcuaoβ mutants also show compromised H2O2-production in guard cells upon MeJA treatment. Furthermore, the H2O2 scavenger N,N1-dimethylthiourea (DMTU) and the CuAO-specific inhibitor 2-bromoethylamine (2-BrEtA) both reversed the MeJA-induced stomatal closure and the H2O2 production in WT plants. Our data suggest that AtCuAOβ is involved in the H2O2 production implicated in MeJA-induced stomatal closure.

Copper amine oxidase and phospholipase D act independently in abscisic acid (ABA)-induced stomatal closure in Vicia faba and Arabidopsis

2014 ◽  
Vol 127 (4) ◽  
pp. 533-544 ◽  
Author(s):  
Yana Qu ◽  
Zhenfeng An ◽  
Baocheng Zhuang ◽  
Wen Jing ◽  
Qun Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Vicia Faba ◽  
Phospholipase D ◽  
Amine Oxidase ◽  
Stomatal Closure ◽  
Copper Amine Oxidase ◽  
Copper Amine
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