Zm401p10, encoded by an anther-specific gene with short open reading frames, is essential for tapetum degeneration and anther development in maize

2009 ◽  
Vol 36 (1) ◽  
pp. 73 ◽  
Author(s):  
Dongxue Wang ◽  
Chengxia Li ◽  
Qian Zhao ◽  
Linna Zhao ◽  
Meizhen Wang ◽  
...  
Keyword(s):  
Zea Mays ◽  
Male Fertility ◽  
Zea Mays L ◽  
Vital Role ◽  
Anther Development ◽  
Flowering Plants ◽  
Open Reading Frames ◽  
Specific Gene ◽  
Upregulated Genes ◽  
Reading Frames

In flowering plants, the tapetum is proposed to play a vital role in the early stages of pollen development. Disruptions to tapetum development and degeneration typically result in male sterility. The present study characterised a maize (Zea mays L.) anther-specific gene, Zm401, which only contains short open reading frames (sORFs). The longest ORF of the Zm401 gene encodes a small protein designated Zm401p10 that accumulates in the nucleus. Overexpression of Zm401p10 in maize retarded tapetal degeneration and caused microspore abnormalities. A microarray analysis identified 278 downregulated and 150 upregulated genes in anthers overexpressing Zm401p10. These results indicate that the Zm401 gene is one of the major components of the molecular network regulating maize anther development and male fertility, and that Zm401p10 is expressed from the longest ORF of the gene.

scholarly journals Genetic dissection of haploid male fertility in maize ( Zea mays L.)

2019 ◽  
Vol 138 (3) ◽  
pp. 259-265 ◽  
Author(s):  
Jiwei Yang ◽  
Yanzhi Qu ◽  
Qiong Chen ◽  
Jihua Tang ◽  
Thomas Lübberstedt ◽  
...  
Keyword(s):  
Zea Mays ◽  
Male Fertility ◽  
Zea Mays L ◽  
Genetic Dissection ◽  
Haploid Male

scholarly journals Genome-Wide Association Study of Haploid Male Fertility in Maize (Zea Mays L.)

2018 ◽  
Vol 9 ◽  
Author(s):  
Hailin Ma ◽  
Guoliang Li ◽  
Tobias Würschum ◽  
Yao Zhang ◽  
Debo Zheng ◽  
...  
Keyword(s):  
Zea Mays ◽  
Association Study ◽  
Male Fertility ◽  
Genome Wide Association Study ◽  
Zea Mays L ◽  
Genome Wide Association ◽  
Genome Wide ◽  
Haploid Male

scholarly journals Structural Characterization of a Specific Glycopeptidolipid Containing a Novel N-Acyl-Deoxy Sugar from Mycobacterium intracellulare Serotype 7 and Genetic Analysis of Its Glycosylation Pathway

2006 ◽  
Vol 189 (3) ◽  
pp. 1099-1108 ◽  
Author(s):  
Nagatoshi Fujiwara ◽  
Noboru Nakata ◽  
Shinji Maeda ◽  
Takashi Naka ◽  
Matsumi Doe ◽  
...  
Keyword(s):  
Amino Alcohol ◽  
Open Reading Frames ◽  
Specific Gene ◽  
Bacterial Physiology ◽  
Mycobacterium Intracellulare ◽  
Host Immune Responses ◽  
Deoxy Sugar ◽  
Glycosylation Pathway ◽  
Reading Frames

ABSTRACT The nontuberculous Mycobacterium avium-Mycobacterium intracellulare complex (MAC) is distributed ubiquitously in the environment and is an important cause of respiratory and lymphatic disease in humans and animals. These species produce polar glycopeptidolipids (GPLs), and of particular interest is their serotype-specific antigenicity. Structurally, GPLs contain an N-acylated tetrapeptide-amino alcohol core that is glycosylated at the C terminal with 3,4-di-O-methyl rhamnose and at the d-allo-threonine with a 6-deoxy-talose. This serotype nonspecific GPL is found in all MAC species. The serotype-specific GPLs are further glycosylated with a variable haptenic oligosaccharide at 6-deoxy-talose. At present, 31 distinct serotype-specific GPLs have been identified on the basis of oligosaccharide composition, and the complete structures of 14 serotype-specific GPLs have been defined. It is considered that the modification of the GPL structure plays an important role in bacterial physiology, pathogenesis, and host immune responses. In this study, we defined the complete structure of a novel serotype 7 GPL that has a unique terminal amido sugar. The main molecular mass is 1,874, and attached to the tetrapeptide-amino alcohol core is the serotype 7-specific oligosaccharide unit of 4-2′-hydroxypropanoyl-amido-4,6-dideoxy-2-O-methyl-β-hexose-(1→3)-α-l-rhamnose-(1→3)-α-l-rhamnose-(1→3)-α-l-rhamnose-(1→2)-α-l-6-deoxy-talose. Moreover, we isolated and characterized the serotype 7-specific gene cluster involved in glycosylation of the oligosaccharide. Nine open reading frames (ORFs) were observed in the cluster. Based on the sequence homology, the ORFs are thought to participate in the biosynthesis of the serotype 7 GPL.

scholarly journals Identification and Characterization of Two Novel Methyltransferase Genes That Determine the Serotype 12-Specific Structure of Glycopeptidolipids of Mycobacterium intracellulare

2007 ◽  
Vol 190 (3) ◽  
pp. 1064-1071 ◽  
Author(s):  
Noboru Nakata ◽  
Nagatoshi Fujiwara ◽  
Takashi Naka ◽  
Ikuya Yano ◽  
Kazuo Kobayashi ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Structural Difference ◽  
Open Reading Frames ◽  
Specific Gene ◽  
Bacterial Physiology ◽  
Mycobacterium Intracellulare ◽  
Functional Analyses ◽  
Identification And Characterization ◽  
Reading Frames

ABSTRACT The Mycobacterium avium complex is distributed ubiquitously in the environment. It is an important cause of pulmonary and extrapulmonary diseases in humans and animals. The species in this complex produce polar glycopeptidolipids (GPLs); of particular interest is their serotype-specific antigenicity. Several reports have described that GPL structure may play an important role in bacterial physiology and pathogenesis and in the host immune response. Recently, we determined the complete structure of the GPL derived from Mycobacterium intracellulare serotype 7 and characterized the serotype 7 GPL-specific gene cluster. The structure of serotype 7 GPL closely resembles that of serotype 12 GPL, except for O methylation. In the present study, we isolated and characterized the serotype 12-specific gene cluster involved in glycosylation of the GPL. Ten open reading frames (ORFs) and one pseudogene were observed in the cluster. The genetic organization of the serotype 12-specific gene cluster resembles that of the serotype 7-specific gene cluster, but two novel ORFs (orfA and orfB) encoding putative methyltransferases are present in the cluster. Functional analyses revealed that orfA and orfB encode methyltransferases that synthesize O-methyl groups at the C-4 position in the rhamnose residue next to the terminal hexose and at the C-3 position in the terminal hexose, respectively. Our results show that these two methyltransferase genes determine the structural difference of serotype 12-specific GPL from serotype 7-specific GPL.

scholarly journals Bacillus safensisFO-36b andBacillus pumilusSAFR-032: A Whole Genome Comparison of Two Spacecraft Assembly Facility Isolates

2018 ◽  
Author(s):  
Madhan R Tirumalai ◽  
Victor G. Stepanov ◽  
Andrea Wünsche ◽  
Saied Montazari ◽  
Racquel O. Gonzalez ◽  
...  
Keyword(s):  
Complete Genome ◽  
Open Reading Frames ◽  
Health Concern ◽  
Genome Comparison ◽  
Specific Gene ◽  
Planetary Protection ◽  
Genome Variability ◽  
Whole Genome Comparison ◽  
Multiple Strains ◽  
Reading Frames

AbstractBackgroundBacillusstrains producing highly resistant spores have been isolated from cleanrooms and space craft assembly facilities. Organisms that can survive such conditions merit planetary protection concern and if that resistance can be transferred to other organisms, a health concern too. To further efforts to understand these resistances, the complete genome ofBacillus safensisstrain FO-36b, which produces spore resistant to peroxide and radiation was determined. The genome was compared to the complete genome ofB. pumilus SAFR-032, as well as draft genomes ofB. safensisJPL-MERTA-8-2 and the type strainB. pumilusATCC7061T. In addition, comparisons were made to 61 draft genomes that have been mostly identified as strains ofB. pumilusorB. safensis.ResultsThe FO-36b gene order is essentially the same as that in SAFR-032 and otherB. pumilusstrains [1]. The annotated genome has 3850 open reading frames and 40 noncoding RNAs and riboswitches. Of these, 307 are not shared by SAFR-032, and 65 are also not shared by either MERTA or ATCC7061T. The FO-36b genome was found to have ten unique reading frames and two phage-like regions, which have homology with theBacillusbacteriophage SPP1 (NC_004166) andBrevibacillusphage Jimmer1 (NC_029104). Differing remnants of the Jimmer1 phage are found in essentially allsafensis/pumilusstrains. Seven unique genes are part of these phage elements. Comparison ofgyrAsequences from FO-36b, SAFR-032, ATCC7061T, and 61 other draft genomes separate the various strains into three distinct clusters. Two of these are subgroups ofB. pumiluswhile the other houses all theB. safensisstrains.ConclusionsIt is not immediately obvious that the presence or absence of any specific gene or combination of genes is responsible for the variations in resistance seen. It is quite possible that distinctions in gene regulation can change the level of expression of key proteins thereby changing the organism’s resistance properties without gain or loss of a particular gene. What is clear is that phage elements contribute significantly to genome variability. The larger comparison of multiple strains indicates that many strains named asB. pumilusactually belong to theB. safensisgroup.

Influence de l'âge sur les caractéristiques photosynthétiques de la feuille de maïs, Zea mays L

Agronomie ◽  
1982 ◽  
Vol 2 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Olivier BETHENOD ◽  
Christine JACOB ◽  
Jean-Claude RODE ◽  
Jean-François MOROT-GAUDRY
Keyword(s):  
Zea Mays ◽  
Zea Mays L

PRODUKTIVITAS PUPUK ORGANIK TERHADAP HASIL TANAMAN JAGUNG (Zea mays L) DI TANAH MINERAL

1970 ◽  
Vol 3 (2) ◽  
pp. 318-326
Author(s):  
Yoyon Riono.
Keyword(s):  
Zea Mays ◽  
Zea Mays L

Penelitian tentang pengaruh pemberian produktivitas pupuk organik terhadap hasil Tanaman Jagung (Zea mays L) di tanah mineral penelitian ini di laksanakan pada bulan Februari sampai Mei, yang bertempat di Sungai Salak Kecsmstsn Tempuling Kabupaten Indragiri Hilir Provinsi Riau. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) yang disusun secara faktorial yang terdiri dari 2 faktor dan 3 ulangan. Faktor B adalah bokashi pupuk kandang yang terdiri dari 4 taraf yaitu B0 (tanpa pemberianpupuk kandang), B1 (5 ton/ha) dan B2 (10 tom/ ha), serta B3 (15 ton/ha) Parameter yang di amati adalah tinggi tanaman, panjang daun ke tujuh, berat brangkasan basah, berat berangkasan kering, berat tongkol pertanaman sampel, diameter tongkol , produksi per plot, dan berat 100 biji. Selanjutnya data yang di peroleh di olah secara statistik, apabila F hitung lebih besar dari F tabel di lanjutkan dengan uji lanjut Tukey HSD pada taraf 5%. Hasil penelitian menunjukan bahwa interaksi bokashi pupuk kandang dan varietas berpengaruh nyata terhadap berat tongkol dan produksi dan produksi per plot, akan tetapi tidak berpengaruh nyata terhadap tinggi tanaman, panjang daun ke tujuh, berat brangkasan basah, berat brangkasan kering , diameter tongkol dan berat 1000 biji. Untuk perlakuan bokashi pupuk kandang secara tunggal berpengaruh nyata terhadap terhadap diameter tongkol , akan tetapi tidak berpengaruh nyata terhadap tinggi tanaman, panjang daun ke tujuh, berat brangkasan basah, berat brangkasan kering, berat tongkol, produksi per plot, dan berat 1000 biji, perlakuan bokashi terbaik terdapat pada pemberian 15 ton/ha. Sedangkan perlakuan varietas secara tunggal berpengaruh nyata terhadap berat brangkasan basah, berat tongkol, dan produksi per plot seta berat 1000 biji, akan tetapi tidak berbeda nyata dengan tinggi tanaman, panjang daun ke tujuh, berat brangkasan kering, dan diameter tongkol. Varietass terbaik adalah NT 10

Relaciones genéticas en muestras de razas mexicanas de maíz (Zea mays L.)

2020 ◽  
Vol 13 (3) ◽  
Author(s):  
Luis Angel Barrera Guzman ◽  
Juan Porfirio Legaria Solano
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Palabras Clave

Objetivo: Caracterizar muestras representativas de razas mexicanas de maíz con marcadores moleculares ISSR, que ayuden a inferir relaciones genéticas vinculadas a su origen, morfología, aspectos ecogeográficos, distribución y usos. Diseño/metodología/aproximación: Se emplearon 54 muestras representativas de razas mexicanas de maíz caracterizadas con diez marcadores moleculares ISSR. Las distancias genéticas se calcularon con el coeficiente Dice y se generó un dendrograma con el método de agrupamiento jerárquico de varianza mínima de Ward. Para visualizar las muestras en dos dimensiones se efectúo un Análisis de Coordenadas Principales con el método de varianza mínima estandarizada. Resultados: En 76 loci detectados, el análisis de agrupamiento con una R2 semiparcial de 0.04 formó cinco grupos que compartieron características filogenéticas, ecogeográficas, morfoagronómicas, de distribución y usos especiales. El análisis de coordenadas principales mostró 21.2 % de la variación total para las dos primeras coordenadas. La primera coordenada principal explicó el 12.43 % de la variación total y separó las muestras por ubicación geográfica y usos especiales; la segunda coordenada principal explicó el 8.77 % de la variación total y separó las muestras por rangos altitudinales y ciclo biológico. Limitaciones del estudio/implicaciones: Se empleó únicamente una muestra representativa por cada raza de maíz, considerando la variabilidad genética de este cultivo se deben incluir más muestras de la misma raza. Hallazgos/conclusiones: Las relaciones genéticas entre las muestras de razas de maíz obedecen a patrones altitudinales y geográficos; aunque algunos agrupamientos compartieron aspectos filogenéticos, morfoagronómicos, de distribución y usos. Palabras clave: Variabilidad genética, recursos fitogenéticos, caracterización molecular, clasificación integral.

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