scholarly journals The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots

2008 ◽  
Vol 35 (12) ◽  
pp. 1224 ◽  
Author(s):  
Matthew O. Jones ◽  
Kenneth Manning ◽  
John Andrews ◽  
Carole Wright ◽  
Ian B. Taylor ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Promoter Sequence ◽  
Specific Gene ◽  
The Novel ◽  
General Applicability ◽  
Root Cortex ◽  
Tomato Root ◽  
Solanum Lycopersicum L ◽  
Gus Reporter ◽  
Root Specificity

Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability. We present the expression characteristics of SlREO, a novel gene isolated from tomato (Solanum lycopersicum L.). This gene was highly expressed in roots but had a very low level of expression in aerial plant organs. A 2.4-kb region representing the SlREO promoter sequence was cloned upstream of the uidA GUS reporter gene and shown to direct expression in the root cortex. In mature, glasshouse-grown plants this strict root specificity was maintained. Furthermore, promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid. The predicted protein sequence of SlREO contains a domain found in enzymes of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily. The novel SlREO promoter has properties ideal for applications requiring strong and specific gene expression in the bulk of tomato root tissue growing in soil, and is also likely to be useful in other Solanaceous crops.

scholarly journals Artificial microRNA-Based RNA Interference and Specific Gene Silencing for Developing Insect Resistance in Solanum lycopersicum

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 136
Author(s):  
Mohammad Faisal ◽  
Eslam M. Abdel-Salam ◽  
Abdulrahman A. Alatar
Keyword(s):  
Rna Interference ◽  
Transgenic Plants ◽  
Gene Silencing ◽  
Solanum Lycopersicum ◽  
Plasmid Vector ◽  
Viral Disease ◽  
Specific Gene ◽  
Artificial Microrna ◽  
Rna Molecules ◽  
Solanum Lycopersicum L

RNA Interference (RNAi), which works against invading nucleic acids or modulates the expression of endogenous genes, is a natural eukaryotic regulating system, and it works by noncoding smaller RNA molecules. Plant-mediated gene silencing through RNAi can be used to develop plants with insect tolerance at transcriptional or post-transcriptional levels. In this study, we selected Myzus persicae’s acetylcholinesterase 1 gene (Ace 1) as a silencing target to develop transgenic Solanum lycopersicum L. plants’ resistance to aphids. An RNAi plasmid vector containing an artificial microRNA (amiRNA) sequence was engineered and successfully transformed into Jamila and Tomaland, two elite tomato cultivars. A northern blot analysis and PCR were carried out to check the efficacy of Agrobacterium-mediated transformation in T0 transgenic plants. The quantitative PCR data showed a substantial downregulation of the Ace 1 gene in aphids fed in clip cages on T1 transgenic plants. Furthermore, there was a substantial drop in aphid colonies that were fed on T1 transgenic plants of both the cultivars. These findings strongly suggest that transgenic plants that express amiRNA could be an important tool for engineering plants resistant to aphids and possibly for the prevention of viral disease in other plant-infested pests.

Evaluación de tres enraizadores comerciales en la producción de plántulas de tomate indeterminado (Solanum lycopersicum (L.) Lam)

2019 ◽  
Vol 12 (12) ◽  
Author(s):  
M. Arébalo-Madrigal ◽  
J.L. Escalante-González ◽  
J.B. Yáñez-Coutiño ◽  
M.E. Gallegos-Castro
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

Objetivo: Evaluar el desarrollo de plántula de tomate indeterminado bajo condiciones protegidas, aplicando  tres enraizadores  y un testigo para aumentar la producción del cultivo en la región. Diseño/metodología/aproximación: se utilizó bajo un diseño experimental en bloques completamente al azar, el cual consistió de cuatro tratamientos correspondiendo a cada uno de los bloques, donde cada bloque pertenecía a cuatro charolas de unicel de 200 cavidades con sustrato de BM2, con cuatro repeticiones cada uno, teniendo 15 unidades experimentales por tratamiento, sumando un total de 60 unidades experimentales, teniendo un total de 240 plántulas de tomate por todo el experimento. Resultados: Como resultado se obtuvo que el enraizador de Phyto Root  tuvo un gran efecto en cuanto al desarrollo de altura, grosor de tallo, numero de hojas, biomasa aérea y peso del cepellón, parámetros importantes que debe tener una plántula para su desarrollo y crecimiento al momento de trasplante a campo. Limitaciones del estudio/implicaciones: El manejo agronómico desde la siembra en charolas, es necesario que sea uniforme en todos los tratamientos y las repeticiones para tener mejores resultados en cuanto el efecto de los enraizadores. Hallazgos/conclusiones: Para obtener plántulas de buena calidad en el momento de trasplante a campo se le recomienda a la empresa y a los productores de plántulas utilizar el tratamiento de Phyto Root, ya que fue la que mejor respuesta tuvo.

Expression of antioxidant genes can increase cold resistance in tomato (Solanum lycopersicum L.)

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Javaria Chand ◽  
Rafiq Ahmad ◽  
Muhammad Shahzad ◽  
Muhammad Sohail Khan ◽  
Noorullah Khan ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Cold Resistance ◽  
Antioxidant Genes ◽  
Solanum Lycopersicum L

Морфо-биологическая и цитологическая характеристика регенерирующих корней томата ( Solanum lycopersicum L.) сорта “Рекордсмен” в условиях NaCl-засоления in vitro

Tsitologiya ◽  
2019 ◽  
Vol 61 (12) ◽  
pp. 998-1013
Author(s):  
Л. Р. Богоутдинова ◽  
Е. Н. Баранова ◽  
Г. Б. Баранова ◽  
Н. В. Кононенко ◽  
Е. М. Лазарева ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

scholarly journals Oligonucleotide array discovery of polymorphisms in cultivated tomato (Solanum lycopersicum L.) reveals patterns of SNP variation associated with breeding

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 466 ◽  
Author(s):  
Sung-Chur Sim ◽  
Matthew D Robbins ◽  
Charles Chilcott ◽  
Tong Zhu ◽  
David M Francis
Keyword(s):  
Solanum Lycopersicum ◽  
Oligonucleotide Array ◽  
Solanum Lycopersicum L

Morpho-physiological investigations in transgenic tomato (Solanum lycopersicum L.) over expressing OsRGLP1 gene

2021 ◽  
Author(s):  
Sumera Perveen ◽  
Tehmina Ahsan Khan ◽  
Humaira Shaheen ◽  
Rabia Naz ◽  
Muhammad Zeeshan Hyder ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Transgenic Tomato ◽  
Solanum Lycopersicum L

scholarly journals Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Antt Htet Wai ◽  
Muhammad Waseem ◽  
A B M Mahbub Morshed Khan ◽  
Ujjal Kumar Nath ◽  
Do Jin Lee ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Solanum Lycopersicum ◽  
Expression Profiling ◽  
Abiotic Stress Tolerance ◽  
Dependent Manner ◽  
Solanum Lycopersicum L ◽  
Genome Wide ◽  
Protein Disulfide

Protein disulfide isomerases (PDI) and PDI-like proteins catalyze the formation and isomerization of protein disulfide bonds in the endoplasmic reticulum and prevent the buildup of misfolded proteins under abiotic stress conditions. In the present study, we conducted the first comprehensive genome-wide exploration of the PDI gene family in tomato (Solanum lycopersicum L.). We identified 19 tomato PDI genes that were unevenly distributed on 8 of the 12 tomato chromosomes, with segmental duplications detected for 3 paralogous gene pairs. Expression profiling of the PDI genes revealed that most of them were differentially expressed across different organs and developmental stages of the fruit. Furthermore, most of the PDI genes were highly induced by heat, salt, and abscisic acid (ABA) treatments, while relatively few of the genes were induced by cold and nutrient and water deficit (NWD) stresses. The predominant expression of SlPDI1-1, SlPDI1-3, SlPDI1-4, SlPDI2-1, SlPDI4-1, and SlPDI5-1 in response to abiotic stress and ABA treatment suggested they play regulatory roles in abiotic stress tolerance in tomato in an ABA-dependent manner. Our results provide new insight into the structure and function of PDI genes and will be helpful for the selection of candidate genes involved in fruit development and abiotic stress tolerance in tomato.

Sign in / Sign up

Export Citation Format

Share Document