Enhanced sensitivity of Arabidopsis anthocyanin mutants to photooxidation: a study with fluorescence imaging

2008 ◽  
Vol 35 (8) ◽  
pp. 714 ◽  
Author(s):  
Ling Shao ◽  
Zhan Shu ◽  
Chang-Lian Peng ◽  
Zhi-Fang Lin ◽  
Cheng-Wei Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Photosynthetic Apparatus ◽  
Chlorophyll Fluorescence Imaging ◽  
Prolonged Treatment ◽  
Partial Recovery ◽  
Wild Type ◽  
Short Term Treatment ◽  
Chlorophyll Fluorescence Parameters

Chlorophyll fluorescence imaging and antioxidative capability in detached leaves of the wild-type Arabidopsis thaliana ecotype Landsberg erecta (Ler) and in three mutants deficient in anthocyanin biosynthesis (tt3, tt4 and tt3tt4) were investigated under photooxidation stress induced by methyl viologen (5 μm) in the light. In comparison with the wild-type (WT) plant, photooxidation resulted in significant decreases in the contents of total phenolics and flavonoid, total antioxidative capability and chlorophyll fluorescence parameters (Fv/Fm, qP, ΦPSII, NPQ and ETR) as determined by chlorophyll fluorescence imaging, and in an increase in cell-membrane leakiness in the three anthocyanin mutants. The sequence of sensitivity to photooxidation in the leaves of the four phenotypes were tt3tt4 (deficient in both chalcone synthase locus (CHS) and dihydroflavonol 4-reductase locus (DFR)) > tt4 (deficient in CHS) > tt3 (deficient in DFR) > WT. The results demonstrate that anthocyanins might, along with other antioxidants, protect the photosynthetic apparatus against photooxidative damage. An interesting phenomenon was observed over the 270 min of the photooxidative treatment, that is, fluorescence imaging revealed that qP, ΦPSII and ETR appeared in three phases (fall → partial recovery → rapid fall). This was considered to be a modulation of reversible deactivation in PSII to cope with the moderate oxidative stress in the first two stages of short-term treatment (<150 min), followed finally by damage to PSII under severe oxidative stress with prolonged treatment.

scholarly journals Misurare la vitalità delle piante per mezzo della fluorescenza della clorofilla

2012 ◽  
Author(s):  
Filippo Bussotti ◽  
Mohamed Hazem Kalaji ◽  
Rosanna Desotgiu ◽  
Martina Pollastrini ◽  
Tadeusz Loboda ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Low Temperatures ◽  
Photosynthetic Apparatus ◽  
Delayed Fluorescence ◽  
Chlorophyll Fluorescence Imaging ◽  
Strong Light ◽  
Fluorescence Techniques ◽  
Modulated Fluorescence ◽  
High And Low Temperatures

The book begins with a detailed description of the characteristics of the photosynthetic apparatus and the processes that take place there, to then present the general principles of fluorescence. After that, it gives a description of the characteristics of direct and modulated fluorescence and a presentation of the shared and distinctive parameters of these two techniques. Then a brief presentation is made of other innovative approaches to the analysis of fluorescence (Chlorophyll Fluorescence Imaging - CFI, P700 absorbance, delayed fluorescence) and the relative tools. An important part of the book comprises a description of the possible applications of fluorescence techniques for the analysis of various types of stress (aridity, strong light radiations, UV, high and low temperatures, salinity, weedkillers, pollution, etc.) and, consequently, their possible use in agriculture, forestry and to protect the environment.

scholarly journals Chlorophyll Fluorescence Imaging-Based Duckweed Phenotyping to Assess Acute Phytotoxic Effects

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2763
Author(s):  
Viktor Oláh ◽  
Anna Hepp ◽  
Muhammad Irfan ◽  
Ilona Mészáros
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Photochemical Efficiency ◽  
Chlorophyll Fluorescence Imaging ◽  
Imaging Method ◽  
Growth Responses ◽  
Chlorophyll Fluorescence Parameters ◽  
Vast Number ◽  
Fluorescence Parameters ◽  
Phytotoxic Effects

Duckweeds (Lemnaceae species) are extensively used models in ecotoxicology, and chlorophyll fluorescence imaging offers a sensitive and high throughput platform for phytotoxicity assays with these tiny plants. However, the vast number of potentially applicable chlorophyll fluorescence-based test endpoints makes comparison and generalization of results hard among different studies. The present study aimed to jointly measure and compare the sensitivity of various chlorophyll fluorescence parameters in Spirodela polyrhiza (giant duckweed) plants exposed to nickel, chromate (hexavalent chromium) and sodium chloride for 72 h, respectively. The photochemistry of Photosystem II in both dark- and light-adapted states of plants was assessed via in vivo chlorophyll fluorescence imaging method. Our results indicated that the studied parameters responded with very divergent sensitivity, highlighting the importance of parallelly assessing several chlorophyll fluorescence parameters. Generally, the light-adapted parameters were more sensitive than the dark-adapted ones. Thus, the former ones might be the preferred endpoints in phytotoxicity assays. Fv/Fm, i.e., the most extensively reported parameter literature-wise, proved to be the least sensitive endpoint; therefore, future studies might also consider reporting Fv/Fo, as its more responsive analogue. The tested toxicants induced different trends in the basic chlorophyll fluorescence parameters and, at least partly, in relative proportions of different quenching processes, suggesting that a basic distinction of water pollutants with different modes of action might be achievable by this method. We found definite hormetic patterns in responses to several endpoints. Hormesis occurred in the concentration ranges where the applied toxicants resulted in strong growth inhibition in longer-term exposures of the same duckweed clone in previous studies. These findings indicate that changes in the photochemical efficiency of plants do not necessarily go hand in hand with growth responses, and care should be taken when one exclusively interprets chlorophyll fluorescence-based endpoints as general proxies for phytotoxic effects.

Screening of growth phases of Antarctic algae and cyanobacteria cultivated on agar plates by chlorophyll fluorescence imaging

2019 ◽  
Vol 9 (2) ◽  
pp. 170-181
Author(s):  
Kristýna Dufková ◽  
Miloš Barták ◽  
Jana Morkusová ◽  
Josef Elster ◽  
Josef Hájek
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Basal Medium ◽  
Photosynthetic Performance ◽  
Chlorophyll Fluorescence Imaging ◽  
Nostoc Commune ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Cultivation Time ◽  
Antarctic Algae

Recently, chlorophyll fluorescence imaging is frequently used non-invasive method to monitor the metabolic state and photosynthetic activities of vascular plants and other autotrophic organisms. In our study, we used the measurements of chlorophyll fluorescence kinetics to follow the development of culture of Antarctic algae (Macrochloris rubrioleum, Zygnema sp.) and cyanobacteria (Hassalia antarctica, Nostoc commune). On the cultures grown on agar plates, Bold´s Basal Medium (BBM), slow Kautsky kinetics supplemented with saturation pulses were measured repeatedly in a week interval. On the kinetics, typical points (OPSMT) were distinguished and species-specific and time of cultivation-dependent differences in shape of the OPSMT kinetics evaluated. We tested sensitivity of various chlorophyll fluorescence parameters to cultivation time on agar plates. In the algae, the most pronounced changes were the decrease in maximum quantum yield of photosystem II (FV/FM) and quenching of basal chlorophyll fluorescence qF0 (M. rubrioleum, Zygnema sp.). In cyanobacteria, chlorophyll fluorescence parameters did not show clear trends with the time of cultivation. F0 quenching (qF0) reached positive values in H. antarctica, while it was negative in N. commune. In both cases, however, qF0 showed an increase with cultivation time. The differences are discussed as well as the potential of the emerging area of the application of chlorophyll fluorescence imaging for evaluation of photosynthetic performance of algal/cyanobacterial cultures on agar plates.

scholarly journals Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2782
Author(s):  
Alyona Grishina ◽  
Oksana Sherstneva ◽  
Marina Grinberg ◽  
Tatiana Zdobnova ◽  
Maria Ageyeva ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Steady State ◽  
Viral Infection ◽  
Fluorescence Imaging ◽  
Potato Virus X ◽  
Chlorophyll Fluorescence Imaging ◽  
Dark Light ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Infected Area

Chlorophyll fluorescence imaging was used to study potato virus X (PVX) infection of Nicotiana benthamiana. Infection-induced changes in chlorophyll fluorescence parameters (quantum yield of photosystem II photochemistry (ΦPSII) and non-photochemical fluorescence quenching (NPQ)) in the non-inoculated leaf were recorded and compared with the spatial distribution of the virus detected by the fluorescence of GFP associated with the virus. We determined infection-related changes at different points of the light-induced chlorophyll fluorescence kinetics and at different days after inoculation. A slight change in the light-adapted steady-state values of ΦPSII and NPQ was observed in the infected area of the non-inoculated leaf. In contrast to the steady-state parameters, the dynamics of ΦPSII and NPQ caused by the dark–light transition in healthy and infected areas differed significantly starting from the second day after the detection of the virus in a non-inoculated leaf. The coefficients of correlation between chlorophyll fluorescence parameters and virus localization were 0.67 for ΦPSII and 0.76 for NPQ. In general, the results demonstrate the possibility of reliable pre-symptomatic detection of the spread of a viral infection using chlorophyll fluorescence imaging.

Detecting crop population growth using chlorophyll fluorescence imaging

2017 ◽  
Vol 56 (35) ◽  
pp. 9762 ◽  
Author(s):  
Heng Wang ◽  
Xiangjie Qian ◽  
Lan Zhang ◽  
Sailong Xu ◽  
Haifeng Li ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Population Growth ◽  
Fluorescence Imaging ◽  
Chlorophyll Fluorescence Imaging
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