N-protein mobilisation associated with the leaf senescence process in oilseed rape is concomitant with the disappearance of trypsin inhibitor activity

2007 ◽  
Vol 34 (10) ◽  
pp. 895 ◽  
Author(s):  
Philippe Etienne ◽  
Marie Desclos ◽  
Lucie Le Gou ◽  
Julie Gombert ◽  
Josette Bonnefoy ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Oilseed Rape ◽  
Leaf Senescence ◽  
Trypsin Inhibitor ◽  
Protein Concentration ◽  
Trypsin Inhibitor Activity ◽  
Brassica Napus L ◽  
Mature Leaves ◽  
Cab Gene ◽  
Young Leaves

Brassica napus L. (oilseed rape) is an important crop plant characterised by low nitrogen (N) use efficiency. This is mainly due to a weak N recycling from leaves that is related to incomplete protein degradation. Assuming that protease inhibitors are involved throughout protein mobilisation, the goal of this study was to determine their role in the control of N mobilisation associated with leaf senescence. Results showed that a 19-kDa polypeptide exhibiting trypsin inhibitor (TI) activity presented an increased gradient from the older to the younger leaves. According to the SAG12/Cab gene expression profile, which is an indicator of leaf senescence, mature leaves of nitrate-deprived plants presented an earlier initiation of senescence and a decrease in protein concentration when compared with nitrate-replete plants. This coincided with disappearance of both TI activity and a reduction in the transcript level of the BnD22 gene (encoding a protein sharing homology with Künitz protease inhibitor). In young leaves of N-deprived plants, initiation of senescence was delayed; soluble protein concentration was maintained while both TI activity and BnD22 transcripts were high. This indicates that in oilseed rape growing under nitrate deprivation, the more efficient N recycling from mature leaves contributes to the maintenance of growth in young leaves. The data suggest a significant role for protease inhibitors in the regulation of proteolytic processes associated with N mobilisation during leaf senescence.

THE INHIBITION OF BRUSSELS SPROUT LEAF SENESCENCE BY KININS

1967 ◽  
Vol 45 (7) ◽  
pp. 1019-1024 ◽  
Author(s):  
D. T. Dennis ◽  
M. Stubbs ◽  
T. P. Coultate
Keyword(s):  
Electron Microscopy ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Leaf Senescence ◽  
Growth Regulators ◽  
Brussels Sprout ◽  
Cell Organelles ◽  
Leaf Discs ◽  
Mature Leaves ◽  
Young Leaves

The senescence of leaf discs cut from mature Brussels sprout leaves is retarded by a number of kinins. Other plant growth regulators are without effect. When young expanding leaves are treated with kinins the senescence processes appear to be accelerated. The effect of N-6-benzyladenine on the structure of the cell organelles in young and old leaves has been studied by electron microscopy. It has been found that in mature leaves the chloroplast is the organelle which senesces first and the breakdown is retarded by N-6-benzyladenine. The mitochondria are very stable. In young leaves N-6-benzyladenine appears to over-stimulate the chloroplast, causing enlargement and excessive membrane synthesis.

Cartier BX summer oilseed rape

2001 ◽  
Vol 81 (1) ◽  
pp. 105-106 ◽  
Author(s):  
S. R. Rimmer ◽  
R. Scarth ◽  
P. B. E. McVetty
Keyword(s):  
Brassica Napus ◽  
Key Words ◽  
Oilseed Rape ◽  
Protein Concentration ◽  
Western Canada ◽  
Blackleg Disease ◽  
Brassica Napus L ◽  
Oil Concentration ◽  
The Mean ◽  
Moderately Resistant

Cartier BX is the fourth in the Navigator® series of summer oilseed rape (Brassica napus L.) canola cultivars, which are resistant to Compas® herbicide, a mix of herbicides including the broadleaf herbicide bromoxynil. It is 32 kg ha–1 (1.3%) higher yielding than the mean of the check cultivars Defender, AC Excel and Legacy, is 5 g kg–1 lower in oil concentration and is 11 g kg–1 higher in protein concentration than the means of the check cultivars, respectively. Cartier BX is moderately resistant to blackleg disease. Cartier BX is adapted to the long- and mid-season B. napus canola-growing areas of western Canada. Key words: Oilseed rape (summer), bromoxynil resistance, Navigator® , Compas®

Light Restriction Delays Leaf Senescence in Winter Oilseed Rape (Brassica napus L.)

2013 ◽  
Vol 32 (3) ◽  
pp. 506-518 ◽  
Author(s):  
Sophie Brunel-Muguet ◽  
Patrick Beauclair ◽  
Marie-Paule Bataillé ◽  
Jean-Christophe Avice ◽  
Jacques Trouverie ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Leaf Senescence ◽  
Winter Oilseed Rape ◽  
Brassica Napus L

The superior nitrogen efficiency of winter oilseed rape (Brassica napus L.) hybrids is not related to delayed nitrogen starvation-induced leaf senescence

2014 ◽  
Vol 384 (1-2) ◽  
pp. 347-362 ◽  
Author(s):  
Fabian Koeslin-Findeklee ◽  
Andreas Meyer ◽  
Andreas Girke ◽  
Katrin Beckmann ◽  
Walter J. Horst
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Leaf Senescence ◽  
Nitrogen Starvation ◽  
Nitrogen Efficiency ◽  
Winter Oilseed Rape ◽  
Brassica Napus L

Transcriptomic analysis of nitrogen starvation- and cultivar-specific leaf senescence in winter oilseed rape ( Brassica napus L.)

Plant Science ◽  
2015 ◽  
Vol 233 ◽  
pp. 174-185 ◽  
Author(s):  
Fabian Koeslin-Findeklee ◽  
Vajiheh Safavi Rizi ◽  
Martin A. Becker ◽  
Sebastian Parra-Londono ◽  
Muhammad Arif ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Leaf Senescence ◽  
Nitrogen Starvation ◽  
Transcriptomic Analysis ◽  
Winter Oilseed Rape ◽  
Brassica Napus L

Renegade BX summer oilseed rape

2002 ◽  
Vol 82 (2) ◽  
pp. 435-436 ◽  
Author(s):  
P. B. E. McVetty ◽  
S. R. Rimmer ◽  
R. Scarth
Keyword(s):  
Brassica Napus ◽  
Key Words ◽  
Oilseed Rape ◽  
Protein Concentration ◽  
Western Canada ◽  
Blackleg Disease ◽  
Brassica Napus L ◽  
Oil Concentration ◽  
Moderately Resistant

Renegade BX is the fifth cultivar in the Navigator® series of summer oilseed rape (Brassica napus L.) canola cultivars. These cultivars are resistant to Compas® herbicide, a mix of herbicides including the broadleaf herbicide bromoxynil. Renegade BX is 380 kg ha-1 (13.6%) higher yielding, 8 g kg-1 higher in oil concentration and equal in protein concentration to the means of the check cultivars Defender, AC Excel and Legacy. Renegade BX is moderately resistant to blackleg disease. Renegade BX is adapted to the long- and mid-season B. napus canola growing areas of western Canada. Key words: Oilseed rape (summer), bromoxynil resistance, Navigator®, Compas®

scholarly journals Effect of cooking in whey on the biological value of beans

2021 ◽  
Vol 37 ◽  
pp. 00051
Author(s):  
Natalia Shamkova ◽  
Asmaa Abdulhamid ◽  
Natalia Bugaets
Keyword(s):  
Protease Inhibitors ◽  
Trypsin Inhibitor ◽  
Control Sample ◽  
Test Organism ◽  
Tetrahymena Pyriformis ◽  
Water Soluble ◽  
Trypsin Inhibitor Activity ◽  
Inhibitor Activity ◽  
Biological Value ◽  
Essential Food

Beans are an essential food and the primary protein source for many people worldwide, and there is a need to develop affordable and nutritious bean-based food products, particularly for individuals who are unable to consume animal protein due to financial or health constraints. This study aimed to investigate how cooking beans in whey can affect the biological value and the activity of protease inhibitors (TIA) of the bean puree obtained. Bean seeds were soaked in water for (5–6) hours, then cooked in the whey after discarding water. Boiled beans were then coarsely ground and followed by fine grinding after removing cooking liquid; the obtained bean puree was cooled to 20 °C. Following the same method, the control sample was prepared using water as a cooking liquid. Bean puree obtained from whey-cooking (BPCW) was compared to control (bean puree obtained from water-cooking (BPW)) in terms of trypsin inhibitor activity (TIA) and the relative biological value (RBV%) using the test organism (Tetrahymena Pyriformis). TIA in BPCW decreased by (6,7 %; 3,8 % – for water soluble and salt soluble TIA respectively). RBV % showed a significant increase (25 %) after treating samples with whey compared to control, demonstrating the efficacy of incorporating bean puree obtained by this method to develop highly nutritious bean-based foodstuffs for specialized and functional purposes.

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