Contribution of anthocyanins to the antioxidant capacity of juvenile and senescing sugar maple (Acer saccharum) leaves

2007 ◽  
Vol 34 (8) ◽  
pp. 714 ◽  
Author(s):  
Abby K. van den Berg ◽  
Timothy D. Perkins
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Acer Saccharum ◽  
Sugar Maple ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Additional Source ◽  
Stable Free Radical ◽  
Maple Leaves

Foliar anthocyanins are hypothesised to provide an additional source of photoprotection from photooxidative stress to the leaves in which they occur through their ability to scavenge excess free radical species. Although demonstrated to significantly enhance the antioxidant status of red morphs of fully expanded leaves of some species, the contribution of anthocyanins to the antioxidant capacity of the juvenile and senescing leaves in which they frequently occur has not been examined. Antioxidant activity of extracts from anthocyanic and non-anthocyanic juvenile and senescing sugar maple (Acer saccharum Marsh.) leaves from similar light environments was assessed using the stable free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH). Anthocyanin content was significantly correlated with antioxidant activity in extracts of anthocyanic juvenile leaves but only weakly correlated in extracts of anthocyanic senescing leaves. In addition, the antioxidant activity of anthocyanic and non-anthocyanic leaves was equal in both juvenile and senescing leaves. Thus, although anthocyanins may contribute to the antioxidant capacity of anthocyanic juvenile and senescing sugar maple leaves, these results are not consistent with the hypothesis that anthocyanins provide an enhancement to the photoprotection available in either leaf type through free radical scavenging. The results suggest anthocyanins may be part of alternative strategies employed by anthocyanic juvenile and senescing maple leaves to achieve similar levels of antioxidant capacity as their non-anthocyanic counterparts to cope with the same set of environmental challenges.

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

scholarly journals Assessment of Physical Stability and Antioxidant Activity of Polysiloxane Polyalkyl Polyether Copolymer-Based Creams

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Atif Ali ◽  
Naveed Akhtar ◽  
Haji Muhammad Shoaib Khan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Power Law ◽  
Plant Extract ◽  
Physical Stability ◽  
Radical Scavenging ◽  
Rheological Parameters ◽  
Ionic Surfactant ◽  
Stable Free Radical ◽  
Free Radical Scavenging Activities

The purpose of the present work was to investigate the changes on physical stability (color, creaming, liquefaction, pH, conductivity, centrifugation, viscosity and rheological parameters) by non-ionic surfactant polysiloxane polyalkyl polyether copolymer based creams following inclusion of plant extract containing phenolic compounds. The antioxidant activity of the plant extract alone and after addition in the cream was assessed using the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Physical stability was assessed by submitting the creams to storage at 8°C, 25°C, 40°C, and at 40°C with 70% RH (relative humidity) for a period of two months. Physical characteristics of polysiloxane polyalkyl polyether copolymer based creams, that is, color, creaming, liquefaction, centrifugation and pH were noted at various intervals for 2 months. The viscosities and rheological behavior of creams were determined using a rotational rheometer. Data were analyzed by using Brookfield Software Rheocalc version (2.6) with IPC Paste and Power Law (PL) math models. Cream with plant extract showed pseudo plastic behaviour with decreasing on viscosity. TheAcacia nilotica(AN) extract alone and the cream containing this extract showed great antioxidant and free radical scavenging activities. Power Law and IPC analysis were found to fit all the rheograms.

scholarly journals Cassava starch edible coating incorporated with propolis on bioactive compounds in strawberries

2016 ◽  
Vol 40 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Ariela Betsy Thomas ◽  
Rita de Cássia Mirela Resende Nassur ◽  
Ana Carolina Vilas Boas ◽  
Luiz Carlos de Oliveira Lima
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Phenolic Compound ◽  
Radical Scavenging ◽  
Biological Properties ◽  
Cassava Starch ◽  
Anthocyanin Content ◽  
Phytochemical Content ◽  
Propolis Extract

ABSTRACT Strawberry is a fruit appreciated throughout the world due to its attractive quality attributes and stands out due to its high phenolic compound content, which positively contribute to biological properties of nutritional interest. The objective of this study was to evaluate the effect of cassava starch coatings incorporated with propolis combinations on the phytochemical content and the maintenance and increase of the strawberry antioxidant activity. The treatments were 3% cassava starch (CS), 3% cassava starch + 33% ethanolic propolis extract (CS + P33%), 3% cassava starch + 66% ethanolic propolis extract (CS + P66%) and control (C). The fruits were stored at 4 °C ± 0.5 ºC and 90%RH for 16 days, making up a completely randomized design with 4 treatments and 5 time evaluations. Vitamin C, phenolic compound, anthocyanin, and antioxidant activity levels were evaluated through two methods. The coating with 66% of propolis promoted higher Vitamin C content than fruits submitted to the other treatments at 8 and 12 days of storage. For antioxidant activity, fruits treated with CS maintained a higher FRS percentage (free radical scavenging) at all time evaluations. Control fruits presented higher anthocyanin content at the last evaluation time when the highest antioxidant capacity, by the ABTS method (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)), was observed in fruits with CS and CS + P66% treatments. There was an increase tendency of the phenolic content during storage in all evaluated fruits. The propolis concentrations used, however, were not sufficient to increase or maintain the antioxidant capacity and phenolic contents of strawberries.

scholarly journals Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

2017 ◽  
Author(s):  
Charalampos Proestos ◽  
Theo Varzakas
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Aromatic Plants ◽  
Polyphenol Content ◽  
Stable Free Radical ◽  
Rancimat Test ◽  
Phenolic Substances

The antioxidant properties and polyphenol content of some selected aromatic plants grown in Greece were studied. Plants were refluxed with 60% methanol after acid hydrolysis. The phenolic substances were quantified by HPLC-DAD. The antioxidant capacity of the extracts was determined with the Rancimat test using sunflower oil as substrate. Free radical scavenging activity was measured using the stable free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Results were compared with standard BHT and ascorbic acid. Total phenol concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard.

scholarly journals Nondestructive Estimation of Anthocyanin Content in Autumn Sugar Maple Leaves

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 685-686 ◽  
Author(s):  
Abby K. van den Berg ◽  
Timothy D. Perkins
Keyword(s):  
Linear Relationship ◽  
Acer Saccharum ◽  
Sugar Maple ◽  
Plant Science ◽  
Anthocyanin Content ◽  
Large Samples ◽  
Maple Leaves ◽  
Content Index ◽  
Color Categories ◽  
Nondestructive Estimation

A field-portable tool for nondestructive foliar anthocyanin content estimation would be beneficial to researchers in many areas of plant science. An existing commercial chlorophyll content meter was modified to measure an index of anthocyanin content. The ability of the experimental anthocyanin meter (ACM) to estimate total extractable anthocyanin content was tested in sugar maple (Acer saccharum Marsh.) leaves representing several subjective color categories collected from a variety of field sites in northwestern Vermont on several dates in Autumn 2003. Overall, there was a significant linear relationship between anthocyanin content index (ACI) and total extractable anthocyanin content (r2 = 0.872, P < 0.001). Therefore, the ACM appears to be an effective tool for estimation of relative anthocyanin content in large samples of autumn sugar maple leaves.

scholarly journals The Nutritional Composition, Antioxidant Activity and Common Phytochemicals of Selected BARI Mango Varieties and Commercial Cultivar Langra

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
Mohammad Mainuddin Molla ◽  
Ashfak Ahmed Sabuz ◽  
Md. Golam Ferdous Chowdhury ◽  
Md. Hafizul Haque Khan ◽  
Mahfujul Alam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Nutritional Composition ◽  
Free Radical Scavenging ◽  
Anthocyanin Content ◽  
Scavenging Activity

The present study sought to explore the nutritional composition, bioactive phytochemicals and antioxidant activity of BARI mango-4, BARI mango-6 and Langra cultivar. The total phenolic (TPH), vitamin C, total carotene, ß-carotene content and antioxidant activity of the mangos were determined by 1,1- diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays (RPA). Phenolic compounds were assessed using high-performance liquid chromatography coupled with a photodiode array detector and auto sampler. Results revealed that moisture, TSS, pH, total acidity, reducing, total sugar and energy of the BARI mango-4 and BARI mango-6 were 76.54 and 75.24 %, 17.10°B and 21.20°B, 4.90 and 5.01, 0.49 and 0.50 %, 3.90 and 4.54 %, 11.20 and 13.46 % and 4028.06 and 3950.27 cal/g respectively whereas the Langra cultivar remained 76.33 %, 17.63°B, 4.25, 0.63 %, 2.79 %, 9.79 % % and 3871.28 cal/g respectively. Phytochemicals especially TPH, ascorbic acid, total flavonoid (TF), total carotenoid (TC), ß-carotene and total anthocyanin content (TAC) of the BARI mango-4 and BARI mango-6 were 20.53 and 20.67 mg GAE/g, 39.98 and 26.26 mg/100 g, 3.14 mg and 2.87 QE/g, 76.38 and 81.33 mg/100 g, 28.17 and 65.84 µg/100 g and 1.67 and 11.69 mg/100 respectively whereas the Langra contained 19.90 mg GAE/g, 25.53 mg/100g, 1.38 mg QE/g, 4.21 mg/100 g, 31.00 µg/100 g and18.22 mg/100 g respectively. In case of antioxidant activities total antioxidant capacity, DPPH radical scavenging activity, reducing power capacity (RPC), metal chelating capacity (MCC), Nitric oxide (NO) free radical scavenging activity and IC50 of the BARI mango-4 and BARI mango-6 were 229.00 and 309.00 µg of ascorbic acid/mg of extract, 96.84 and 94.73 %, 12.20 and 9.71 µg/mL, 157.36 and 132.89 %, 61.74 and 72.65 µg/mL and 0.59 and 0.71 µg/mL respectively whereas the Langra cultivar contained 194.25 µg of ascorbic acid/mg of extract, 87.94 %, 2.54 µg/mL, 177.80 %, 53.74 µg/mL and 25.11 µg/mL respectively. The results indicate that BARI mango-4 and BARI mango-6 exhibited rich source of TPH, TC, ß-carotene, ascorbic acid, TA, TAC and NO free radical scavenging activity whereas the Langra is the rich source of MCC and anthocyanin content. Phenolic acids were leading agent in BARI mango-4 and BARI mango-6. Moreover, BARI mango-4 and BARI mango-6 extract had a great potential to fight free radical chain reactions and for usage in therapeutic applications.

scholarly journals Efektifitas Frekuensi Ekstraksi Serta Pengaruh Suhu dan Cahaya Terhadap Antosianin dan Daya Antioksidan Ekstrak Kelopak Rosella (Hibiscus sabdariffa L.)

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Suharyani Amperawati ◽  
Pudji Hastuti ◽  
Yudi Pranoto ◽  
Umar Santoso
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Light Exposure ◽  
Radical Scavenging ◽  
Effect Of Temperature ◽  
Anthocyanin Content ◽  
Extraction Temperature ◽  
Hibiscus Sabdariffa ◽  
Total Anthocyanin ◽  
Dpph Method

Penelitian ini bertujuan untuk mengetahui kualitas dan kadar antosianin ekstrak kelopak rosela dari perlakuan ekstraksi dengan maserasi pada beberapa kali ekstraksi, serta mempelajari pengaruh suhu dan pemaparan cahaya selama penyimpanan terhadap kadar antosianin dan daya antioksidan. Ekstraksi kelopak rosela dilakukan sebanyak 4 kali dengan metode maserasi. Pengaruh penyimpanan dengan suhu 30, 40, 50, 60 dan 70oC selama 7, 14, 21, 28, dan 35 hari. Pemaparan cahaya dilakukan dengan 1478, 2835, dan 3940 lux dan sebagai pembanding digunakan perlakuan tanpa pencahayaan selama 1 sampai dengan 10 hari. Parameter untuk ektraksi meliputi kadar antosianin, warna (L*, a*, b*, ΔE), dan total padatan terlarut. Parameter penyimpanan meliputi kadar antosianin dan daya antioksidan metode radical scavenging (DPPH). Hasil peneltian menunjukkan bahwa ekstraksi ke 1, 2, 3, dan 4 kali menunjukkan kadar antosianin masing-masing 608, 218, 64, dan 32 mg/l; nilai L* sebesar 29,07; 32,27; 36,19; dan 45,27; nilai a* sebesar 10,42; 16,33; 21,90 dan 15,63; dan b* adalah 1,36; 4,56; 8,33; dan 5,86; ΔE adalah 1,31; 7,53; 15,29; dan 18,06.  Ekstraksi lebih baik dilakukan 2 kali, sedangkan ekstraksi yang ke-3 dan 4 menghasilkan kadar antosianin yang relatif kecil. Hasil penelitian juga menunjukkan bahwa makin tinggi suhu dan lama penyimpanan menyebabkan kandungan antosianin dan kapasitas antioksidan makin menurun, demikian pula makin tinggi intensitas cahaya menyebabkan makin menurunnya kadar antosianin dan kemampuan antioksidannya. Kesimpulannya, berdasarkan semua parameter yang diukur maka ekstraksi dapat dilakukan sampai dengan 2 kali dan guna menjaga antosianin dan antioksidannya, maka sebaiknya disimpan dalam ruang dingin dan terhindar dari cahaya matahari.Kata kunci : rosela, ekstraksi, suhu, cahaya, antioksidan Extraction Frequency Effectiveness and Effect of Temperature and Light on Anthocyanin and Antioxidant Capacity of Rosella Petal Extract (Hibiscus sabdariffa L.)Abstract         This study aims to determine the quality and levels of anthocyanin roselle petal extract from extraction treatment with maceration frequency and to determine the effect of temperature and light exposure during storage on anthocyanin levels and antioxidant activity. Roselle petal extraction was carried out 4 times with maceration method. Storage condition were set up at 30, 40, 50, 60, and 70oC for 7, 14, 21, 28, and 35 days. Light exposure was conducted using 1478, 2835, and 3940 lux and as a comparison, the extract was kept without light from 1 to 10 days. The parameters for extraction were anthocyanin, color levels (L*, a*, b*, ΔE), and total soluble solids. Storage parameters were anthocyanin content and antioxidant capacity of the radical scavenging (DPPH) method. The results of anthocyanin levels of extraction 1, 2, 3, and 4 times were 608, 218, 64, and 32 mg/L; 29.07, 32.27, 36.19, and 45.27 for L*, 10.42, 16.33, 21.90 and 15.63 for a* value, 1.36, 4.56, 8.33, and 5.86 for b* value, 1,31; 7,53; 15,29; and 18,06 for ΔE, respectively. Twice extraction was provide much better result for total anthocyanin, while 3rd and 4th extractions produced relatively small level of anthocyanin. The results also showed that the higher temperature and storage time, the decrease in anthocyanin content and antioxidant capacity. The higher intensity of the light caused reduction of anthocyanin content and its antioxidant activity. As conclusion, roselle extract might be conducted for two times. The storage was also suggested at low temperatures and low exposure light to keep its anthocyanin and antioxidant.Keywords: rosella, extraction, temperature, light, antioxidant

scholarly journals Relationships between Structure and Antioxidant Capacity and Activity of Glycosylated Flavonols

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 849
Author(s):  
Zhengcao Xiao ◽  
Liangliang He ◽  
Xiaohui Hou ◽  
Jianping Wei ◽  
Xiaoyu Ma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Sulfonic Acid ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hydroxyl Group ◽  
Dpph Assay ◽  
Dimer Formation ◽  
Abts Assay

The antioxidant capacity (AC) and antioxidant activity (AA) of three flavonols (FLV), aglycones and their glycosylated derivatives were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays in various solvents. Findings confirmed that the glycosylation at the 3-position (3-glycosylation) always decreased the AC under most conditions due to substitution of the 3-position hydroxyl group and glycoside disruption in the molecular planarity. The 7-glycosylated derivatives did not have the above effects, thus generally exhibited ACs similar to their aglycones. Glycosylation decreased the AA of kaempferol and isorhamnetin for both assays in methanol, 3-glycosylation inhibited quercetin AA in the ABTS assay. In the DPPH assay, the AA of 3-glycosylated quercetin was significantly higher than quercetin. Using LC–MS/MS analysis, we found that quercetin and quercetin-7-glucoside underwent dimerization during the antioxidant reaction, potentially leading to a decline in AAs. However, 3-glycoside substitution may have hindered dimer formation, thereby allowing the FLVs to retain strong free radical scavenging abilities.

scholarly journals Characterization of Phytoconstituents from Alcoholic Extracts of Four Woody Species and Their Potential Uses for Management of Six Fusarium oxysporum Isolates Identified from Some Plant Hosts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1325
Author(s):  
Mohamed Z. M. Salem ◽  
Abeer A. Mohamed ◽  
Hayssam M. Ali ◽  
Dunia A. Al Al Farraj
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Leaf Extracts ◽  
Conium Maculatum

Background: Trees are good sources of bioactive compounds as antifungal and antioxidant activities. Methods: Management of six molecularly identified Fusarium oxysporum isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from Conium maculatum leaves, Acacia saligna bark, Schinus terebinthifolius wood and Ficus eriobotryoides leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene-linoleic acid (BCB) bleaching assays. Results: In mg/kg extract, the highest amounts of polyphenolic compounds p-hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in C. maculatum leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in A. saligna bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in S. terebinthifolius wood extract; and rutin, o-coumaric acid, p-hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in F. eriobotryoides leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of F. oxysporum strains showed that A. saligna bark followed by C. maculatum leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from C. maculatum leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching. Conclusions: Extracts from A. saligna bark and C. maculatum leaves are considered potential candidates against the growth of F. oxysporum isolates—a wilt pathogen—and C. maculatum leaf as a potent antioxidant agent.

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