Research Note: The five families of sucrose-phosphate synthase genes in Saccharum spp. are differentially expressed in leaves and stem

2006 ◽  
Vol 33 (6) ◽  
pp. 605 ◽  
Author(s):  
C. P. L. Grof ◽  
C. T. E. So ◽  
J. M. Perroux ◽  
G. D. Bonnett ◽  
R. I. Forrester
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Gene Families ◽  
Type B ◽  
Saccharum Spp ◽  
Mature Leaves ◽  
Type D ◽  
Key Enzyme ◽  
And Function ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose-phosphate synthase (SPS) is a key enzyme in the pathway of sucrose synthesis. Five different gene families encoding SPS have been reported in the Poaceae [Castleden CK, Aoki N, Gillespie VJ, MacRae EA, Quick WP, Buchner P, Foyer CH, Furbank RT, Lunn JE (2004) Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Plant Physiology 135, 1753–1764]. Expression of the five families in leaf and stem tissues of Saccharum spp. at different stages of development was determined by quantitative real-time PCR. The type B and C families of SPS genes were predominantly expressed in both immature and mature leaves, whereas the two subfamilies making up the type D family were expressed at similar levels in all tissues examined. In the type A family, expression was lowest in leaves and increased from the meristem region down to internode 7 of the stem.

scholarly journals Evolution and Function of the Sucrose-Phosphate Synthase Gene Families in Wheat and Other Grasses

2004 ◽  
Vol 135 (3) ◽  
pp. 1753-1764 ◽  
Author(s):  
C. Kate Castleden ◽  
Naohiro Aoki ◽  
Vanessa J. Gillespie ◽  
Elspeth A. MacRae ◽  
W. Paul Quick ◽  
...  
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Gene Families ◽  
And Function ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

scholarly journals TRANSFORMASI GENETIK DAN EKSPRESI MUTAN SUCROSE PHOSPHATE SYNTHASE PADA TANAMAN TOMAT

2019 ◽  
Vol 6 (1) ◽  
pp. 130
Author(s):  
Suwinda Fibriani ◽  
Inyana Dwi Agustien ◽  
Widhi Dyah Sawitri ◽  
Bambang Sugiharto
Keyword(s):  
Genetic Transformation ◽  
Tomato Plant ◽  
Sucrose Phosphate Synthase ◽  
Transgenic Tomato ◽  
Pcr Analysis ◽  
N Terminus ◽  
Key Enzyme ◽  
Sucrose Biosynthesis ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman.  Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik 

A modified assay method shows leaf sucrose-phosphate synthase activity is correlated with leaf sucrose content across a range of sugarcane varieties

1998 ◽  
Vol 25 (4) ◽  
pp. 499 ◽  
Author(s):  
Christopher P.L. Grof ◽  
Deon P. Knight ◽  
Scott D. McNeil ◽  
John E. Lunn ◽  
James A. Campbell
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Assay Method ◽  
Sucrose Content ◽  
Saccharum Spp ◽  
Sugarcane Varieties ◽  
Controlled Conditions ◽  
The Cross ◽  
First Time ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Eight different commercial and breeding varieties of sugarcane (Saccharum spp.) grown in controlled conditions were assayed for leaf sucrose-phosphate synthase (SPS) (EC 2.1.4.14) activity and leaf sucrose content. Leaf SPS activity measured at 25˚C ranged between 0.06 and 0.14 nmol sucrose formed mg protein -1 min-1. The cross-varietal average for leaf SPS activity was 0.10 nmol µg protein-1 min-1 (equivalent to 63.4 µmol h-1 g FW-1 or 17.6 nkat g FW-1) which is consistent with previously published leaf SPS activities for sugarcane; however, previous studies have assayed leaf SPS activity at either 30 or 37˚C. The range of leaf sucrose content across varieties (5.5–18.0 mg sucrose g FW-1, average 11.3 mg g FW-1) was consistent with all but one of four previously published reports. Leaf SPS activity and leaf sucrose content were significantly correlated across the eight varieties examined (r2 =0.877, d.f. =7,P<0.001). Whilst previous reports have indicated a co-relationship between leaf SPS activity and leaf sucrose content in single sugarcane varieties both diurnally and with different nutrient regimes, this study shows, for the first time, that this co-relationship also holds true across a range of sugarcane varieties.

scholarly journals Structure and Expression Analysis of Sucrose Phosphate Synthase, Sucrose Synthase and Invertase Gene Families in Solanum lycopersicum

2021 ◽  
Vol 22 (9) ◽  
pp. 4698
Author(s):  
Yaoke Duan ◽  
Lan Yang ◽  
Haijia Zhu ◽  
Jie Zhou ◽  
Hao Sun ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Sucrose Synthase ◽  
Sucrose Phosphate Synthase ◽  
Gene Families ◽  
Tissue Expression ◽  
Tissue Expression Pattern ◽  
Structure Characteristics ◽  
Invertase Gene ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and invertase (INV) are all encoded by multigene families. In tomato (Solanum lycopersicum), a comprehensive analysis of structure characteristics of these family genes is still lacking, and the functions of individual isoforms of these families are mostly unclear under stress. Here, the structure characteristics of the three families in tomato were analyzed; moreover, as a first step toward understanding the functions of isoforms of these proteins under stress, the tissue expression pattern and stress response of these genes were also investigated. The results showed that four SPS genes, six SUS genes and nineteen INV genes were identified in tomato. The subfamily differentiation of SlSPS and SlSUS might have completed before the split of monocotyledons and dicotyledons. The conserved motifs were mostly consistent within each protein family/subfamily. These genes demonstrated differential expressions among family members and tissues, and in response to polyethylene glycerol, NaCl, H2O2, abscisic acid or salicylic acid treatment. Our results suggest that each isoform of these families may have different functions in different tissues and under environmental stimuli. SlSPS1, SlSPS3, SlSUS1, SlSUS3, SlSUS4, SlINVAN5 and SlINVAN7 demonstrated consistent expression responses and may be the major genes responding to exogenous stimuli.

scholarly journals Sucrose Phosphate Synthase, a Key Enzyme for Sucrose Biosynthesis in Plants

1991 ◽  
Vol 96 (2) ◽  
pp. 473-478 ◽  
Author(s):  
Jean-Michel Bruneau ◽  
Ann C. Worrell ◽  
Bernard Cambou ◽  
Danielle Lando ◽  
Toni A. Voelker
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Key Enzyme ◽  
Sucrose Biosynthesis ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

scholarly journals Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 200 ◽  
Author(s):  
Risky Mulana Anur ◽  
Nurul Mufithah ◽  
Widhi Dyah Sawitri ◽  
Hitoshi Sakakibara ◽  
Bambang Sugiharto
Keyword(s):  
Sugar Content ◽  
Sucrose Phosphate Synthase ◽  
Sucrose Content ◽  
Transgenic Sugarcane ◽  
Soluble Acid Invertase ◽  
Sugarcane Yield ◽  
Transgenic Lines ◽  
Key Enzyme ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose phosphate synthase (SPS) is a key enzyme in sucrose synthesis, which controls sucrose content in plants. This study was designed to examine the efficacy of the overexpression of SoSPS1 gene on sucrose accumulation and carbon partitioning in transgenic sugarcane. The overexpression of SoSPS1 gene increased SPS activity and sucrose content in transgenic sugarcane leaves. More importantly, the overexpression enhanced soluble acid invertase (SAI) activity concomitant with the increase of glucose and fructose levels in the leaves, whereas sucrose synthase activity exhibited almost no change. In the stalk, a similar correlation was observed, but a higher correlation was noted between SPS activity and sugar content. These results suggest that SPS overexpression has both direct and indirect effects on sugar concentration and SAI activity in sugarcane. In addition, SPS overexpression resulted in a significant increase in plant height and stalk number in some transgenic lines compared to those in non-transgenic control. Taken together, these results strongly suggest that enhancing SPS activity is a useful strategy for improving sugarcane yield.

scholarly journals Identification and expression profile analysis of the sucrose phosphate synthase gene family in Litchi chinensis Sonn.

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4379 ◽  
Author(s):  
Dan Wang ◽  
Jietang Zhao ◽  
Bing Hu ◽  
Jiaqi Li ◽  
Yaqi Qin ◽  
...  
Keyword(s):  
Gene Family ◽  
Profile Analysis ◽  
Functional Divergence ◽  
Expression Patterns ◽  
Sucrose Phosphate Synthase ◽  
Gene Families ◽  
Specific Expression ◽  
Litchi Chinensis ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose phosphate synthase (SPS, EC 2.4.1.14) is a key enzyme that regulates sucrose biosynthesis in plants. SPS is encoded by different gene families which display differential expression patterns and functional divergence. Genome-wide identification and expression analyses of SPS gene families have been performed in Arabidopsis, rice, and sugarcane, but a comprehensive analysis of the SPS gene family in Litchi chinensis Sonn. has not yet been reported. In the current study, four SPS gene (LcSPS1, LcSPS2, LcSPS3, and LcSPS4) were isolated from litchi. The genomic organization analysis indicated the four litchi SPS genes have very similar exon-intron structures. Phylogenetic tree showed LcSPS1-4 were grouped into different SPS families (LcSPS1 and LcSPS2 in A family, LcSPS3 in B family, and LcSPS4 in C family). LcSPS1 and LcSPS4 were strongly expressed in the flowers, while LcSPS3 most expressed in mature leaves. RT-qPCR results showed that LcSPS genes expressed differentially during aril development between cultivars with different hexose/sucrose ratios. A higher level of expression of LcSPS genes was detected in Wuheli, which accumulates higher sucrose in the aril at mature. The tissue- and developmental stage-specific expression of LcSPS1-4 genes uncovered in this study increase our understanding of the important roles played by these genes in litchi fruits.

scholarly journals Cloning, Expression and Genetic Transformation of Sucrose Phosphate Synthase (Sps) Gene in Saccharum Spontaneum L.

2017 ◽  
Vol 59 (2) ◽  
pp. 7-15
Author(s):  
Yun-Wei Zhang ◽  
Yun-Zhuan Zhou ◽  
Hai-Bo Lu ◽  
Deng-Yu Zheng ◽  
Yan-Hua Huang
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Saccharum Spontaneum ◽  
Reading Frame ◽  
Acid Protein ◽  
Significant Difference ◽  
Key Enzyme ◽  
Young Leaves ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

AbstractSucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.

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