The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light

2006 ◽  
Vol 33 (1) ◽  
pp. 9 ◽  
Author(s):  
Dušan Lazár
Keyword(s):  
Electron Transport ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
High Intensity ◽  
High Temperature Stress ◽  
Electron Acceptors ◽  
Exciting Light ◽  
Electric Voltage ◽  
Light Gradient ◽  
Fluorescence Rise

Chlorophyll a fluorescence rise caused by illumination of photosynthetic samples by high intensity of exciting light, the O–J–I–P (O–I1–I2–P) transient, is reviewed here. First, basic information about chlorophyll a fluorescence is given, followed by a description of instrumental set-ups, nomenclature of the transient, and samples used for the measurements. The review mainly focuses on the explanation of particular steps of the transient based on experimental and theoretical results, published since a last review on chlorophyll a fluorescence induction [Lazár D (1999) Biochimica et Biophysica Acta 1412, 1–28]. In addition to ‘old’ concepts (e.g. changes in redox states of electron acceptors of photosystem II (PSII), effect of the donor side of PSII, fluorescence quenching by oxidised plastoquinone pool), ‘new’ approaches (e.g. electric voltage across thylakoid membranes, electron transport through the inactive branch in PSII, recombinations between PSII electron acceptors and donors, electron transport reactions after PSII, light gradient within the sample) are reviewed. The K-step, usually detected after a high-temperature stress, and other steps appearing in the transient (the H and G steps) are also discussed. Finally, some applications of the transient are also mentioned.

Research note: On the intermediate steps between the O and P steps in chlorophyll a fluorescence rise measured at different intensities of exciting light

2001 ◽  
Vol 28 (11) ◽  
pp. 1151 ◽  
Author(s):  
Pavel Tomek ◽  
Dusan Lazár ◽  
Petr Ilík ◽  
Jan Naus
Keyword(s):  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
High Intensity ◽  
Electron Acceptors ◽  
Exciting Light ◽  
Low Intensity ◽  
Fluorescence Rise ◽  
Fluorescence Level ◽  
Wheat Leaves ◽  
The Mathematical Model

It is known that the characteristic O–I–P transient of the fast chlorophyll a fluorescence rise (FR) detected under low intensity of exciting light, changes to the O–J–I–P (O–I 1 –I 2 –P) transient under higher intensity of exciting light. In this work, we extend an application of the mathematical model of FR [Lazár et al. (1997)l Pesticide Biochemistry and Physiology 57, 200–210], involving photosystem II (PSII) heterogeneity and simulating the O–J–I–P transient, for modeling of FR under different intensities of exciting light. Our simulations qualitatively agree well with experimental FR curves obtained with wheat leaves. The simulations demonstrate that the first step after the O fluorescence level (the I step and the J step under lower and higher intensities of exciting light, respectively) is caused mainly by the accumulation of reduced QA electron acceptors without reduction of subsequent electron acceptors. The first step appears at shorter times with increasing intensity of exciting light as reported previously [Strasser et al. (1995) Photochemistry and Photobiology 61, 32–42]. Our simulations also demonstrate, in accordance with previous publications, that the O–I phase in FR detected under low intensity of exciting light, reflects mainly the accumulation of Q A – in the Q B-non-reducing PSII centers and partly also the accumulation of Q A – in the Q B -reducing PSII centers. In our model, the accumulation of Q A – in the Q B -non-reducing PSII centers gradually shifts with increasing intensity of exciting light to shorter times and participates in the formation of the O–J phase; this is also supported by measurements of FR with wheat leaves treated with 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU). Under high intensity of exciting light, the O–J phase in FR also markedly reflects the accumulation of Q A – in the Q B -reducing PSII centers. Our simulations also support the previously reported suggestion of Strasser’s group that the I step in FR obtained under high intensity of exciting light (in the position of the I step detected under lower intensity of exciting light) appears mainly owing to the accumulation of the Q A –Q B 2– and Q A–Q BH 2f orms.

scholarly journals Effects of Different Planting Densities on Photosynthesis in Maize Determined via Prompt Fluorescence, Delayed Fluorescence and P700 Signals

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 276
Author(s):  
Wanying Chen ◽  
Bo Jia ◽  
Junyu Chen ◽  
Yujiao Feng ◽  
Yue Li ◽  
...  
Keyword(s):  
Electron Transport ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Electron Transport Chain ◽  
Plant Density ◽  
Photosynthetic Electron Transport ◽  
Planting Density ◽  
Strong Impact ◽  
Photosynthetic Electron Transport Chain ◽  
Transport Chain

The mutual shading among individual field-grown maize plants resulting from high planting density inevitably reduces leaf photosynthesis, while regulating the photosynthetic transport chain has a strong impact on photosynthesis. However, the effect of high planting density on the photosynthetic electron transport chain in maize currently remains unclear. In this study, we simultaneously measured prompt chlorophyll a fluorescence (PF), modulated 820 nm reflection (MR) and delayed chlorophyll a fluorescence (DF) in order to investigate the effect of high planting density on the photosynthetic electron transport chain in two maize hybrids widely grown in China. PF transients demonstrated a gradual reduction in their signal amplitude with increasing planting density. In addition, high planting density induced positive J-step and G-bands of the PF transients, reduced the values of PF parameters PIABS, RC/CSO, TRO/ABS, ETO/TRO and REO/ETO, and enhanced ABS/RC and N. MR kinetics showed an increase of their lowest point with increasing high planting density, and thus the values of MR parameters VPSI and VPSII-PSI were reduced. The shapes of DF induction and decay curves were changed by high planting density. In addition, high planting density reduced the values of DF parameters I1, I2, L1 and L2, and enhanced I2/I1. These results suggested that high planting density caused harm on multiple components of maize photosynthetic electron transport chain, including an inactivation of PSII RCs, a blocked electron transfer between QA and QB, a reduction in PSI oxidation and re-reduction activities, and an impaired PSI acceptor side. Moreover, a comparison between PSII and PSI activities demonstrated the greater effect of plant density on the former.

Acclimation of shade-tolerant and light-resistant Tradescantia species to growth light: chlorophyll a fluorescence, electron transport, and xanthophyll content

2017 ◽  
Vol 133 (1-3) ◽  
pp. 87-102 ◽  
Author(s):  
Vladimir I. Mishanin ◽  
Boris V. Trubitsin ◽  
Svetlana V. Patsaeva ◽  
Vasily V. Ptushenko ◽  
Alexei E. Solovchenko ◽  
...  
Keyword(s):  
Electron Transport ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence

Oxygen-quenched chlorophyll a fluorescence and electron transport in barley during greening

1984 ◽  
Vol 62 (3) ◽  
pp. 344-348 ◽  
Author(s):  
R. Popovic ◽  
D. Fraser ◽  
W. Vidaver ◽  
K. Colbow
Keyword(s):  
Electron Transport ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence
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