Eucalyptus grandis has at least two functional SOC1-like floral activator genes

2004 ◽  
Vol 31 (3) ◽  
pp. 225 ◽  
Author(s):  
John M. Watson ◽  
Elizabeth M. Brill
Keyword(s):  
Flowering Time ◽  
Sequence Data ◽  
Ectopic Expression ◽  
Eucalyptus Grandis ◽  
Mads Box ◽  
Wild Type ◽  
Mads Box Genes ◽  
Nucleotide Sequence Data ◽  
Transgenic Lines ◽  
Young Leaves

In a search for Eucalyptus grandis Hill ex Maiden MADS-box genes involved in floral initiation and development, we isolated two cDNAs (EgrMADS 3 and EgrMADS 4), which are functional orthologues of the Arabidopsis thaliana (L.) Heynh. floral activator gene SOC1. These two E. grandis genes are equally and most-actively expressed in vegetative tissues such as apical shoots and young leaves. The two genes are less actively, but differentially expressed in roots and unopened flowers. Ectopic expression of EgrMADS 3 or EgrMADS 4 in the late-flowering A. thaliana Ler soc1 (agl20) derivative complemented the phenotype of this mutant, and some of these transgenic lines flowered significantly earlier than the wild-type Ler ecotype. Overexpression of EgrMADS 3 or EgrMADS 4 in A. thaliana ecotype Columbia accelerated flowering time under short-day conditions. However, under the same conditions the flowering time of A. thaliana ecotype C24 was altered by the ectopic expression of EgrMADS 3, but not that of EgrMADS 4.The nucleotide sequence data reported will appear in the EMBL and GenBank Nucleotide Databases under the accession numbers AY263807 (EgrMADS 3) and AY263808 (EgrMADS 4).

Ectopic expression of a Eucalyptus grandis SVP orthologue alters the flowering time of Arabidopsis thaliana

2004 ◽  
Vol 31 (3) ◽  
pp. 217 ◽  
Author(s):  
Elizabeth M. Brill ◽  
John M. Watson
Keyword(s):  
Arabidopsis Thaliana ◽  
Sequence Data ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Eucalyptus Grandis ◽  
Floral Organ ◽  
Sequence Alignments ◽  
Nucleotide Sequence Data ◽  
Mads Box Gene ◽  
Nucleotide Database

A new MADS-box gene, EgrSVP was isolated from vegetative tips of Eucalyptus grandis Hill ex Maiden saplings. This gene was expressed in vegetative tissues such as shoots, leaves and roots, as well as in unopened floral buds. DNA sequence alignments indicate that EgrSVP shares the highest level of sequence identity with PkMADS1, JOINTLESS, IbMADS3 and SVP. Phylogenetically, it is grouped in the JOINTLESS clade, the members of which share similar expression patterns. Transgenic Arabidopsis thaliana (L.) Heynh. plants overexpressing EgrSVP, exhibited a variety of altered phenotypes, including homeotic floral organ transformation, indeterminate floral development, multiple inflorescences and coflorescences, and some degree of late flowering.The nucleotide sequence data reported will appear in the GenBank Nucleotide Database under the accession number AY263809.

scholarly journals EjFRI, FRIGIDA (FRI) Ortholog from Eriobotrya japonica, Delays Flowering in Arabidopsis

2020 ◽  
Vol 21 (3) ◽  
pp. 1087
Author(s):  
Weiwei Chen ◽  
Peng Wang ◽  
Dan Wang ◽  
Min Shi ◽  
Yan Xia ◽  
...  
Keyword(s):  
Flowering Time ◽  
Ectopic Expression ◽  
Eriobotrya Japonica ◽  
Flower Initiation ◽  
Wild Type ◽  
Expression Levels ◽  
Functional Roles ◽  
Significant Difference ◽  
Transgenic Lines ◽  
Delayed Flowering

In the model species Arabidopsis thaliana, FRIGIDA (FRI) is a key regulator of flowering time and can inhibit flowering without vernalization. However, little information is available on the function in the Rosaceae family. Loquat (Eriobotrya japonica) belongs to the family Rosaceae and is a distinctive species, in which flowering can be induced without vernalization, followed by blooming in late-autumn or winter. To investigate the functional roles of FRI orthologs in this non-vernalization species, we isolated an FRI ortholog, dubbed as EjFRI, from loquat. Analyses of the phylogenetic tree and protein sequence alignment showed that EjFRI is assigned to eurosids I FRI lineage. Expression analysis revealed that the highest expression level of EjFRI was after flower initiation. Meanwhile, EjFRI was widely expressed in different tissues. Subcellular localization of EjFRI was only detected to be in the nucleus. Ectopic expression of EjFRI in wild-type Arabidopsis delayed flowering time. The expression levels of EjFRI in transgenic wild-type Arabidopsis were significantly higher than those of nontransgenic wild-type lines. However, the expression levels of AtFRI showed no significant difference between transgenic and nontransgenic wild-type lines. Furthermore, the upregulated AtFLC expression in the transgenic lines indicated that EjFRI functioned similarly to the AtFRI of the model plant Arabidopsis. Our study provides a foundation to further explore the characterization of EjFRI, and also contributes to illuminating the molecular mechanism about flowering in loquat.

Influence of ectopic expression of Asteraceae MADS box genes on plant ontogeny in tobacco

2011 ◽  
Vol 109 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Elena N. Goloveshkina ◽  
Anna V. Shchennikova ◽  
Anastasia M. Kamionskaya ◽  
Konstantin G. Skryabin ◽  
Olga A. Shulga
Keyword(s):  
Ectopic Expression ◽  
Mads Box ◽  
Mads Box Genes ◽  
Plant Ontogeny

Transgenic lines of Begonia maculata generated by ectopic expression of PttKN1

Biologia ◽  
2011 ◽  
Vol 66 (2) ◽  
Author(s):  
Quan-le Xu ◽  
Jiang-ling Dong ◽  
Nan Gao ◽  
Mei-yu Ruan ◽  
Hai-yan Jia ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Ectopic Expression ◽  
Class I ◽  
Wild Type Plant ◽  
Wild Type ◽  
Knox Gene ◽  
Knox Genes ◽  
Pttkn1 Gene ◽  
Transgenic Lines ◽  
First Time

AbstractKNOX (KNOTTED1-like homeobox) genes encode homeodomain-containing transcription factors which play crucial roles in meristem maintenance and proper patterning of organ initiation. PttKN1 gene, isolated from the vascular cambium of hybrid aspen (Populus tremula × P. tremuloides), is a member of class I KNOX gene family. In order to understand the roles of PttKN1 gene in meristem activity and morphogenesis as well as to explore the possibility to generate novel ornamental lines via its ectopic expression, it was introduced into the genome of Begonia maculata Raddi by Agrobacterium tumefasciens-mediated gene transformation here. Four types of transgenic plants were observed, namely coral-like (CL) type, ectopic foliole (EF) type, phyllotaxy-irregular (IP) type and cup-shaped (CS) type, which were remarkably different from corresponding wild type and were not also observed in the regenerated plantlets of wild type plant. Among these four types of transgenic plants, the phenotype of coral-like was observed for the first time in the transformants ectopically expressed KNOX genes. The observation of scanning electron microscope (SEM) showed ectopic meristems on the adaxial leaf surface of the transformants. Interestingly, the plantlets with ectopic foliole could generate new ectopic folioles from the original ectopic folioles again, and the plants regenerated from the EF-type transformants could also maintain the original morphology. The same specific RT-PCR band of the four types of transgenic plantlets showed that PttKN1 was ectopically expressed. All these data demonstrated that the ectopic expression of PttKN1 caused a series of alterations in morphology which provided possibilities producing novel ornamental lines and that PttKN1 played important roles in meristem initiation, maintenance and organogenesis events as other class I KNOX genes.

scholarly journals Extending the Toolkit for Beauty: Differential Co-Expression of DROOPING LEAF-Like and Class B MADS-Box Genes during Phalaenopsis Flower Development

2021 ◽  
Vol 22 (13) ◽  
pp. 7025
Author(s):  
Francesca Lucibelli ◽  
Maria Carmen Valoroso ◽  
Günter Theißen ◽  
Susanne Nolden ◽  
Mariana Mondragon-Palomino ◽  
...  
Keyword(s):  
Differential Expression ◽  
Flower Development ◽  
Differential Expression Analysis ◽  
Mads Box ◽  
Wild Type ◽  
Mads Box Genes ◽  
Regulatory Interactions ◽  
Class B ◽  
Gene Regulatory ◽  
Orchid Flower

The molecular basis of orchid flower development is accomplished through a specific regulatory program in which the class B MADS-box AP3/DEF genes play a central role. In particular, the differential expression of four class B AP3/DEF genes is responsible for specification of organ identities in the orchid perianth. Other MADS-box genes (AGL6 and SEP-like) enrich the molecular program underpinning the orchid perianth development, resulting in the expansion of the original “orchid code” in an even more complex gene regulatory network. To identify candidates that could interact with the AP3/DEF genes in orchids, we conducted an in silico differential expression analysis in wild-type and peloric Phalaenopsis. The results suggest that a YABBY DL-like gene could be involved in the molecular program leading to the development of the orchid perianth, particularly the labellum. Two YABBY DL/CRC homologs are present in the genome of Phalaenopsis equestris, PeDL1 and PeDL2, and both express two alternative isoforms. Quantitative real-time PCR analyses revealed that both genes are expressed in column and ovary. In addition, PeDL2 is more strongly expressed the labellum than in the other tepals of wild-type flowers. This pattern is similar to that of the AP3/DEF genes PeMADS3/4 and opposite to that of PeMADS2/5. In peloric mutant Phalaenopsis, where labellum-like structures substitute the lateral inner tepals, PeDL2 is expressed at similar levels of the PeMADS2-5 genes, suggesting the involvement of PeDL2 in the development of the labellum, together with the PeMADS2-PeMADS5 genes. Although the yeast two-hybrid analysis did not reveal the ability of PeDL2 to bind the PeMADS2-PeMADS5 proteins directly, the existence of regulatory interactions is suggested by the presence of CArG-boxes and other MADS-box transcription factor binding sites within the putative promoter of the orchid DL2 gene.

The study of the E-class SEPALLATA3-like MADS-box genes in wild-type and mutant flowers of cultivated saffron crocus (Crocus sativus L.) and its putative progenitors

2011 ◽  
Vol 168 (14) ◽  
pp. 1675-1684 ◽  
Author(s):  
Athanasios Tsaftaris ◽  
Konstantinos Pasentsis ◽  
Antonios Makris ◽  
Nikos Darzentas ◽  
Alexios Polidoros ◽  
...  
Keyword(s):  
Crocus Sativus ◽  
Mads Box ◽  
Wild Type ◽  
Mads Box Genes ◽  
Crocus Sativus L

MPF2-Like MADS-Box Genes Affecting Expression of SOC1 and MAF1 are Recruited to Control Flowering Time

2012 ◽  
Vol 54 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Muhammad Ramzan Khan ◽  
Irfan Ullah Khan ◽  
Ghulam Muhammad Ali
Keyword(s):  
Flowering Time ◽  
Mads Box ◽  
Mads Box Genes ◽  
Control Flowering Time
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