Artificial infection by endophytes affects growth and mycorrhizal colonisation of Lolium perenne

2003 ◽  
Vol 30 (4) ◽  
pp. 419 ◽  
Author(s):  
Joachim Müller
Keyword(s):  
Lolium Perenne ◽  
Mycorrhizal Fungi ◽  
Root Biomass ◽  
Arbuscular Mycorrhizal Fungus ◽  
Fungal Endophytes ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Artificial Infection ◽  
Neotyphodium Lolii ◽  
Mycorrhizal Colonisation

Fungal endophytes of the Clavicipitaceae live in aboveground parts of many grasses of temperate regions. Seedlings of two different cultivars of perennial ryegrass, Lolium perenne L., were artificially infected with one of two endophytes, Epichloë typhina and Neotyphodium lolii, or mock infected. The seedlings were then grown in a mixture of vermiculite and sand in the presence or absence of an inoculum of an arbuscular mycorrhizal fungus (Sclerocystis sp.). Ten weeks later, the plants were harvested and analysed with respect to mycorrhizal colonisation and shoot and root biomass. Endophyte-infected plants showed a significant decrease of mycorrhizal colonisation. This decrease was in some cases correlated with alterations of growth. Depending on the endophyte strains and on the mycorrhizal status, shoot–root biomass ratios were significantly affected. For both L. perenne cultivars, arbuscular mycorrhiza formation led to a higher shoot–root biomass ratio in N. lolii-infected plants and, conversely, to a lower shoot–root ratio in E. typhina-infected plants. These results indicate that effects of endophytes may be enhanced or counterbalanced in the presence of arbuscular mycorrhizal fungi.

scholarly journals The methylome of the model arbuscular mycorrhizal fungus, Rhizophagus irregularis, shares characteristics with early diverging fungi and Dikarya

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anurag Chaturvedi ◽  
Joaquim Cruz Corella ◽  
Chanz Robbins ◽  
Anita Loha ◽  
Laure Menin ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Plant Species ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Rhizophagus Irregularis ◽  
Functional Differences ◽  
Phosphate Regulation ◽  
Very High

AbstractEarly-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glomeromycotina) evolved a symbiotic lifestyle with 80% of plant species worldwide. Here we show that these fungi exhibit 5mC and 6mA methylation characteristics that jointly set them apart from other fungi. The model AMF, R. irregularis, evolved very high levels of 5mC and greatly reduced levels of 6mA. However, unlike the Dikarya, 6mA in AMF occurs at symmetrical ApT motifs in genes and is associated with their transcription. 6mA is heterogeneously distributed among nuclei in these coenocytic fungi suggesting functional differences among nuclei. While far fewer genes are regulated by 6mA in the AMF genome than in EDF, most strikingly, 6mA methylation has been specifically retained in genes implicated in components of phosphate regulation; the quintessential hallmark defining this globally important symbiosis.

In vitro culture of arbuscular mycorrhizal fungus and Frankia for inoculation of micropropagated Casuarina equisetifolia L.

1999 ◽  
Vol 77 (9) ◽  
pp. 1391-1397
Author(s):  
Genevieve Louise Mark ◽  
John E Hooker ◽  
Alexander Hahn ◽  
Chris T Wheeler
Keyword(s):  
Spore Germination ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Dichlorophenoxyacetic Acid ◽  
Casuarina Equisetifolia ◽  
Half Strength ◽  
2,4 Dichlorophenoxyacetic Acid

Micropropagated, rooted, and calli explants of Casuarina equisetifolia L. were inoculated with Frankia UGL 020605S and the arbuscular mycorrhizal fungus (AMF) Glomus mosseae, in single and dual co-culture, in vitro. Different micropropagation media formulations were evaluated for their capacity to stimulate germination of G. mosseae spores and growth of Frankia. Murashige and Skoog basal nutrient (half strength) medium, supplemented with 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), and pyruvate was selected for the in vitro co-culture of C. equisetifolia callus explants, G. mosseae, and Frankia. This medium (M4) supported 70% AMF spore germination with 44 and 34% of the germinating spores producing single and branched hyphal strands, respectively. Hoaglands (quarter strength, modified by Hoaglands and Arnon (1950)) nutrient medium (M5) with no supplements was selected for the in vitro co-culture of rooted C. equisetifolia explants, G. mosseae, and Frankia and supported 57% AMF spore germination with 29 and 40% of the germinating spores producing single and branched hyphal strands, respectively. Both media supported significant growth of Frankia. In both cases agar was substituted with Terragreen(r). AMF appressoria and intercellular hyphae were observed in rooted C. equisetifolia at 28 days; arbuscule formation occurred at 56 days postinoculation. Frankia infection was evident after 28 days. This was observed in both dual and single in vitro co-cultures. No specific immunofluorescent or immunogold reactions to monoclonal antibodies (mABs) anti-Frankia < 8C5 > and anti-G. mosseae < F5G5 > were evident in C. equisetifolia callus explants.Key words: arbuscular mycorrhizal fungi (AMF), Frankia, Casuarina, micropropagation, immunofluorescent labelling.

scholarly journals Arbuscular mycorrhizal fungi and auxin associated with microelements in the development of cuttings of Varronia leucocephala

2019 ◽  
Vol 23 (3) ◽  
pp. 167-174 ◽  
Author(s):  
Mônica D. S. da S. Fernandes ◽  
Marciana B. de Morais ◽  
Francisco F. Mesquita-Oliveira ◽  
Cláudia Ulisses ◽  
José F. de Medeiros ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Aerial Part ◽  
Root Formation ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Spore Density ◽  
Number Of Leaves ◽  
Therapeutic Properties

ABSTRACT The plant Varronia leucocephala is widely used in Brazil for its therapeutic properties. However, a major problem for the seedlings is the low percentage of root formation. The objective of this study was to establish a rooting protocol for V. leucocephala cuttings, using phytoregulators and microelements associated with arbuscular mycorrhizal fungi. The auxin indole-3-butyric acid (IBA) concentration of 1500 mg L-1 showed the best rooting percentage, and it is proposed associating the microelements zinc and boron with the highest IBA doses. Although an increase in the rooting percentage was observed in the presence of zinc, it was not the most suitable for improving the percentage of propagation. Consequently, association of arbuscular mycorrhizal fungi with 1500 mg L-1 IBA plus zinc was selected to evaluate the rooting percentage and sprouting of the aerial part, dry biomass of roots and aerial part, number of leaves, height, mycorrhizal colonization and dependency, spore density, and nutrients of branches and roots. These results show that using zinc with the highest doses of IBA (1500 mg L-1) in plants inoculated with the arbuscular mycorrhizal fungus (Gigaspora albida) was the most effective at promoting the vegetative propagation of V. leucocephala.

Effects of vesicular-arbuscular mycorrhizal infection in first, second and third cereal crops

1985 ◽  
Vol 105 (3) ◽  
pp. 631-647 ◽  
Author(s):  
J. G. Buwalda ◽  
D. P. Stribley ◽  
P. B. Tinker
Keyword(s):  
Spring Wheat ◽  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Residual Effects ◽  
Cereal Crops ◽  
Mycorrhizal Infection ◽  
Vesicular Arbuscular

SUMMARYThe effects of inoculation with the vesicular-arbuscular mycorrhizal fungus Olomics mosseae(Nicolson & Gerdemann) Gerdemann and Trappe, fumigation of soil with methyl bromide, and addition of superphosphate (up to 60 kg P/ha) on growth and phosphorus nutrition of spring wheat (Triticum aestivum L. cv. Highbury) were investigated in two experiments (in 1980 and 1981 respectively) on plots that had been fallowed and recently limed.Fumigation severely reduced natural levels of infection, and slightly reduced yield of above-ground dry matter in both years. In 1981 a decrease in grain yield of about 25% was accompanied by an increase in growth of straw. Plants on fumigated plots contained appreciable amounts of bromine in shoot tissue.Inoculation increased and added P decreased infection in all treatments. In 1980 inoculation had little effect on above-ground dry matter, but it increased concentration of P in shoots especially on plots without added P. In 1981 added inoculum increased yield of grain on fumigated plots by about 0·75 t/ha at all levels of added P, but had little effect on non-fumigated plots, though responses in grain production to added P were similar with and without fumigation. Increases in yield resulting from inoculation were generally accompanied by increases in concentration of P in plant tissue.Winter barley was sown on the plots after their use for spring wheat, without further application of the fumigation, inoculation or phosphorus treatments used in those experiments, to determine any residual effects on mycorrhizal infection and on growth. The levels of mycorrhizal infection on non-fumigated, inoculated plots were relatively constant in successive crops, although numbers of propagules of mycorrhizal fungi increased significantly with time for all treatments. Infection levels on fumigated and non-inoculated plots increased in successive crops, so that the relative effects of fumigation and of inoculation declined with time.The effects of inoculation on infection levels persisted for longer than those on yields, suggesting that maximum effects of mycorrhizal infection on growth did not require the maximum levels of infection found in the roots. Harvest yields continued to respond to applied phosphorus even when uniformly high levels of infection had been established, suggesting that the ability of the root system to absorb phosphate was not greatly increased by mycorrhizal infection.

scholarly journals Impact of AMF Claroideoglomus etunicatum on the structure of fungal communities in the tomato rhizosphere

2019 ◽  
Vol 54 (1) ◽  
Author(s):  
Agnieszka Jamiołkowska ◽  
Ali Hamood Thanoon ◽  
Elżbieta Patkowska ◽  
Jarosław Grządziel
Keyword(s):  
Mycorrhizal Fungi ◽  
Soil Microorganisms ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Fungal Communities ◽  
Saprotrophic Fungi ◽  
Tomato Seedlings ◽  
Tomato Cultivars ◽  
Object Of Study

Mycorrhizal fungi influence the development and activity of communities of soil microorganisms. The purpose of this study was to estimate the effect of arbuscular mycorrhizal fungus <em>Claroideoglomus etunicatum</em> (W. N. Becker &amp; Gerd.) C. Walker &amp; Schüβler on the population structure of fungal colonies in the rhizosphere of tomatoes grown in a plastic tunnel. The field experiment was conducted from 2015 to 2017 at an ecological farm in Grądy, central eastern Poland. The object of study were the three tomato cultivars: ‘Antalya F<sub>1</sub>’, ‘Esmira F<sub>1</sub>’, and ‘Pelikan F<sub>1</sub>’. Tomato seedlings were inoculated with <em>C. etunicatum</em>; spores were introduced about 5 cm deep in the rhizosphere of the studied plants (25–30 spores of <em>C. etunicatum</em> for each plant). Each year, mycological analysis of the tomato rhizosphere was conducted using Warcup’s method; structure of fungal communities of the tomato rhizosphere varied depending on the AMF applied. Saprotrophic fungi such as <em>Trichoderma</em> ssp., <em>Mucor</em> spp., and <em>Penicillium</em> spp. were often more isolated from the rhizosphere of plants inoculated with <em>C. etunicatum</em> than that of the control samples. It can be concluded that AMF directly impacted the development of fungal biodiversity in the tomato rhizosphere, particularly regarding the number of saprotrophs in the soil.

scholarly journals Suppression of Pratylenchus brachyurus and soybean growth inoculated with arbuscular mycorrhizal fungus

2021 ◽  
Vol 43 ◽  
pp. e3
Author(s):  
Edicarla Trentin ◽  
Valéria Ortaça Portela ◽  
Juliane Schmitt ◽  
Reyllis Kiefer Unfer ◽  
Zaida Inês Antoniolli ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Mycorrhizal Colonization ◽  
Dry Matter Accumulation ◽  
Pratylenchus Brachyurus ◽  
Presence And Absence ◽  
Soybean Plants

Arbuscular mycorrhizal fungi perform a variety of plant-beneficial processes. including increased resistance to disease. The objective of this work was to study arbuscular mycorrhizal fungus Rhizoglomus clarum effect on phytonematode Pratylenchus brachyurus suppression and on soybean plants growth. Two experiments were performed under greenhouse conditions. First. soybean plants growth was evaluated in mycorrhizal fungi presence and absence. In the second experiment. phytonematode damage in soybean cultivated in mycorrhizal fungi presence and absence was evaluated. During soybean flowering was evaluated mycorrhizal colonization, dry matter, nodulation, chlorophyll and nutrient content in plant tissue, nematodes number in soil and root penetration, and nematode reproduction factor was obtained, R. clarum mycorrhizal colonization reduced by 64% the number of nematodes penetrated in roots and increased soybean plants nodulation, nutrient absorption and dry matter accumulation. The stimulation to mycorrhization is a strategy to reduce damage caused by Pratylenchus brachyurus to soybean plants.

scholarly journals The Vicia faba Leghemoglobin Gene VfLb29 Is Induced in Root Nodules and in Roots Colonized by the Arbuscular Mycorrhizal Fungus Glomus fasciculatum

1997 ◽  
Vol 10 (1) ◽  
pp. 124-131 ◽  
Author(s):  
Martin Frühling ◽  
Hélène Roussel ◽  
Vivienne Gianinazzi-Pearson ◽  
Alfred Pühler ◽  
Andreas M. Perlick
Keyword(s):  
Gene Expression ◽  
Vicia Faba ◽  
Mycorrhizal Fungi ◽  
Broad Bean ◽  
Arbuscular Mycorrhizal Fungus ◽  
Root Nodules ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Glomus Fasciculatum ◽  
Symbiotic Interactions

To investigate similarities between symbiotic interactions of broad bean (Vicia faba) with rhizobia and mycorrhizal fungi, plant gene expression induced by both microsymbionts was compared. We demonstrated the exclusive expression of 19 broad bean genes, including VfENOD2, VfENOD5, VfENOD12 and three different leghemoglobin genes, in root nodules. In contrast, the leghemoglobin gene VfLb29 was found to be induced not only in root nodules, but also in broad bean roots colonized by the mycorrhizal fungus Glomus fasciculatum. In uninfected roots, none of the 20 nodulin transcripts investigated was detectable. VfLb29 has an unusually low sequence homology with all other broad bean leghemoglobins as well as with leghemoglobins from other legumes. It can be regarded as a novel kind of leghemoglobin gene not described until now and the induction of which is common to symbiotic interactions of broad bean with both Rhizobium and a mycorrhizal fungus.

scholarly journals Seed exudates of Sesbania virgata (Cav.) Pers. stimulate the asymbiotic phase of the arbuscular mycorrhizal fungus Gigaspora albida Becker & Hall

Hoehnea ◽  
2019 ◽  
Vol 46 (1) ◽  
Author(s):  
Leilyane C.S. Coelho ◽  
Daiane S.B. Mignoni ◽  
Fábio S.B. Silva ◽  
Márcia R. Braga
Keyword(s):  
Secondary Metabolites ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Mycelial Growth ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Sesbania Virgata ◽  
Positive Effect ◽  
Gigaspora Albida

ABSTRACT Sesbania virgata is a legume used in the restoration of degraded areas and forms a symbiosis with arbuscular mycorrhizal fungi (AMF). Its seeds exude secondary metabolites that may influence the colonization by AMF. In this work, we studied the effects of seed (SE) and root exudates (RE) of S. virgata on the asymbiotic phase of Gigaspora albida. Spores of G. albida were germinated in medium supplemented with different concentrations of SE or RE. After seven days, spore germination was stimulated (46.6%) in the medium supplemented with the highest concentration of SE, while the mycelial growth was stimulated with the lowest SE concentration. In turn, RE had no effect on the fungal asymbiotic phase. We concluded that SE exert a positive effect on the asymbiotic phase of G. albida and that the different effects between SE and RE of S. virgata can be explained by their distinct content of secondary metabolites.

scholarly journals Enhanced Rooting of Kinnikinnick Cuttings using Mycorrhizal Fungi in Rooting Substrate

2004 ◽  
Vol 14 (3) ◽  
pp. 355-363 ◽  
Author(s):  
C.F. Scagel
Keyword(s):  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Root Initiation ◽  
Cutting Propagation ◽  
Different Types ◽  
Rooting Of Cuttings ◽  
Bottom Heat

Hardwood cuttings of kinnikinnick (Arctostaphylos uva-ursi `Massachusetts') were inoculated with three different types of inoculum of mycorrhizal fungi to determine whether addition of mycorrhizal inoculum into the rooting substrate during cutting propagation increases rooting or root growth, or alters the time for rooting. Cuttings, treated or untreated with rooting hormone prior to sticking into the rooting substrate, were inoculated with either inoculum of an arbuscular mycorrhizal fungus (AMF), hyphal inoculum of an arbutoid mycorrhizal fungus (E), or inoculum consisting of colonized root fragments of kinnikinnick (R). Cuttings were placed under mist in a greenhouse with no bottom heat and harvested 35, 56, and 84 days after sticking. Using AMF inoculum in the rooting substrate did not enhance rooting of cuttings, while adding the R or E inoculum to the rooting substrate increased root initiation compared to non-inoculated cuttings. Cuttings inoculated with either the R or E inoculum had greater root initiation than non-inoculated cuttings 56 and 84 days after sticking. When treated with rooting hormone, cuttings inoculated with the E or R inoculum had longer roots and a greater root biomass than non-inoculated cuttings. Mycorrhizal colonization of roots was similar or greater when cuttings were inoculated with the E inoculum than with the R inoculum and application of rooting hormone generally increased root colonization. The use of inoculum composed of root fragments from kinnikinnick during cutting propagation does not appear to be more beneficial than use of hyphal inoculum from a known arbutoid mycorrhizal fungus.

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