Assessment of soybean seed vigour tests for eastern Australia

1996 ◽  
Vol 36 (1) ◽  
pp. 99
Author(s):  
M Boersma ◽  
M Law ◽  
SW Adkins

Vigour testing was undertaken on seed from 4 soybean [Glycine max (L.) Merr.] cultivars taken from field trials in eastern Australia. The seed from each cultivar exhibited moderate to good germination and a range in vigour. Vigour tests consisted of the controlled deterioration and modified versions of the accelerated aging test. Accelerated aging was carried out in 4 ways using 2 periods of incubation (76 and 96 h), with or without the seed protectant Thiram to control saprophytic fungi. Results were compared with seedling emergence from the same seed samples when planted in a controlled environment glasshouse. A combination of 2 day/night temperatures, cold (18/13�C), warm (33/28�C), and 3 soil moisture regimes, dry (-0.3 MPa), field capacity (-0.01 MPa) and saturated soil, produced a total of 6 different environments. Emergence was also recorded under a moderate daylnight temperature of 23/18�C in soil at field capacity. The standard germination test and modified accelerated aging test with a 72-h incubation period with Thiram were significantly (P<0.01) correlated with emergence from all 7 soil environments. A 96-h period of accelerated aging (with or without Thiram) tended to suppress germination and there was no correlation with emergence from most soil environments. The controlled deterioration test correlated with seedling emergence from most soil environments except for dry soil. The vigour test which gave the best indicator of performance under a range of different seedbed moisture and temperature conditions was the modified accelerated aging test using a 72-h incubation period with Thiram.

1996 ◽  
Vol 36 (1) ◽  
pp. 93 ◽  
Author(s):  
SW Adkins ◽  
M Boersma ◽  
M Law

Laboratory vigour tests were performed on seed from 4 soybean [Glycine max (L.) Merr.] cultivars coming from commercial crops grown in eastern Australia. The seed from each cultivar had greater than 80% germination and a range in vigour. Vigour tests consisted of the accelerated aging (with or without a seed protectant, Thiram), cold, saturation and hypocotyl length tests. Results were compared with seedling emergence from the same cultivar when planted under growth room conditions (25/20 � 1�C, 14/10 h day/night) at 3 seedbed moisture levels (field capacity, -0.01 MPa; dry, -0.30 MPa; and saturated soil, soil saturated for 48 h, planted then allowed to dry to field capacity). The modified accelerated aging test with Thiram was significantly (P<0.01) correlated with emergence in all 3 seedbed moisture conditions. High vigour seed samples with high accelerated aging germinations emerged well. Overall, the vigour test which ranked the seed samples to give the best indicator of performance under a range of seedbed moisture conditions was the modified accelerated aging test with thiram.


2014 ◽  
Vol 36 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Juliana Tieme Yagushi ◽  
Denis Santiago Costa ◽  
José de Barros França-Neto

Seed deterioration causes losses to seed companies during the storage. For researches, the challenge has been to develop techniques which improve the seed longevity as well as laboratory analysis methods required for evaluating the evolution of deterioration in storage. This study had the objective of determining the effectiveness of the accelerated aging test with a saturated NaCl solution and Seed Vigor Imaging System (SVIS) analysis in checking soybean seed physiological in comparison with the recommended vigor tests. Soybean seeds from the cultivars BRS 184 and M-Soy 7908 RR were used, each of them were represented by six lots and stored for six months under two environment conditions. Seed physiological quality was checked every two months in storage through germination evaluations, accelerated aging (different methods), tetrazolium test (vigor and viability) seedling emergence, and SVIS analysis. The tetrazolium test is the most advisable method for evaluating soybean seed quality during storage. The results of the saturated salt accelerated aging test with NaCl at 41 ºC for 72 or 96 hours and the SVIS evaluation are also both efficient for estimating the physiological potential of seeds.


2013 ◽  
Vol 35 (3) ◽  
pp. 284-291
Author(s):  
Maria Angélica Lodo ◽  
Magnólia de Mendonça Lopes ◽  
Bruna Resende Ribeiro Soares ◽  
Roberval Daiton Vieira

The efficiency of vigor tests in assessing the physiological potential of seeds depends on their standardization for different species. In this context, the research aimed to study specific methodologies to evaluate the vigor of spinach seeds (Tetragonia tetragonoides). For this purpose, five lots of spinach seed cv. New Zealand were submitted to germination, first count of germination, seedling emergence in the field, accelerated aging (with and without use of saturated solution of NaCl at 41and 45 ºC for 24,48 and 72 hours), controlled deterioration (18,21 and 24% seed water content adjustments for 24 hours at 45 ºC) and electrical conductivity tests with variations inwater volume (25, 50 and 75 mL), seed quantity (25 and 50) and soaking period (1, 2, 4, 8,12, 16 and 24 hours) at 25 ºC. The first count test allowed obtaining preliminary information about seed vigor, and other tests in general showed similarity with seedling emergence in the field. Thus, it was concluded that traditional accelerated aging test 24 h /41 ºC and accelerated aging test with saturated salt solution for 24 h at 41 ºC and 45 ºC, and controlled deterioration test with 21% seed water content / 24 h / 45 ºC were all efficient for evaluating the physiological potential of spinach seeds.


2003 ◽  
Vol 60 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Angelica Brod Rodo ◽  
Julio Marcos Filho

International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L.) seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41ºC / 48 and 72 h), controlled deterioration (24% of water / 45ºC / 24 h) and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41ºC / 48 and 72 h) and controlled deterioration (moisture content adjusted to 24% / 45ºC / 24 h) tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.


2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Clíssia Barboza da Silva ◽  
Rafael Marani Barbosa ◽  
Roberval Daiton Vieira

ABSTRACT: This study aimed to determine the most appropriate method to assess the sunn hemp ( Crotalaria juncea L.) seed vigor in the accelerated aging test. Five seed lots from harvest 2007/2008 were evaluated for germination, vigor and seedling emergence in the field. Accelerated aging test was performed at 41°C during 48, 72 and 96 hours, with and without sodium chloride saturated solution. Then, the promising procedure was also performed for 2008/2009 and 2009/2010 harvests. In the traditional accelerated aging test, the no-uniform water acquisition by the seeds adversely affected results. Accelerated aging test with sodium chloride saturated solution is the most appropriate method to evaluate the physiological potential of sunn hemp seeds, and the combination of 41°C and 96 hours is efficient for separating of seed lots in different vigor levels.


2021 ◽  
Vol 42 (2) ◽  
pp. 455-470
Author(s):  
Vanderléia Mathias ◽  
◽  
Cileide Maria Medeiros Coelho ◽  

Although the accelerated aging test is widely used to determine seed vigor, there are methodological variations in seed exposure time at 41 °C. Therefore, the objective was to indicate the most appropriate seed exposure time to segregate lots that also exhibits a correlation with seedling emergence in the field, and establish vigor levels using the aforementioned test. Soybean seed samples were collected at pre-sowing, representing 100 lots in the 2016/2017 growing season and 125 lots in the 2017/2018 season from different warehouses in 4 regions of Santa Catarina state (SC). Physiological quality was evaluated by germination, accelerated aging (24 and 48 h at 41 °C) and field emergence tests. The results were submitted to descriptive statistical analysis and Pearson’s correlation analysis (p < 0.05). The rank sum index, associated with the Scott-Knott clustering method, was used to classify physiological quality. The average germination percentage was 90 and 91% in the 2016/2017 and 2017/2018 growing seasons, respectively. Vigor obtained an average of 86 (2016/2017) and 85% (2017/2018), when seeds were submitted to accelerated aging for 48 h, and 89% (both seasons) for 24 h. In regard to field emergence, the average was 85 and 80% in the 2016/2017 and 2017/2018 growing seasons, respectively. Considering the 225 lots, the greatest correlation with field emergence was observed in vigor - 48 h (r = 0.71 p < 0.05). Rank sum classification made it possible to establish vigor-based categories, whereby batches with over 85% vigor by 48-h accelerated aging were classified as high vigor, 70 to 84% medium vigor, and under 70% low vigor.


2021 ◽  
Author(s):  
A. A. C. B. Alahakoon ◽  
D. S. de Z. Abeysiriwardena ◽  
J. W. Damunupola ◽  
F. R. Hay ◽  
N. S. Gama-Arachchige

2015 ◽  
Vol 37 (3) ◽  
pp. 234-240 ◽  
Author(s):  
Carla Regina Baptista Gordin ◽  
Silvana de Paula Quintão Scalon ◽  
Tathiana Elisa Masetto

Abstract:Niger is a promising oilseed species for biodiesel production but there is no much information about the physiological potential of its seeds. Thus, the aim was to adapt the methodologies of accelerated aging test on six lots of niger seeds. The test was carried out by traditional and with saturated salt solution (20 and 40 g NaCl.100 mL-1) methods at 41 and 45 °C for 24, 48, 72 and 96 hours. After the decay period, the seeds were submitted to the germination test, proceeding to an evaluation on the seventh day after sowing, counting the normal seedlings percentage. A completely randomized design with four replications of 50 seeds was used and the means were compared by Tukey's test. The accelerated aging test was correlated with seedling emergence and provided lots classification in at least two levels of vigor. For the accelerated aging test, the method with 20 g NaCl.100 mL-1 at 41 °C for 24 hours is recommend. The traditional method is not suitable because it provides water content variation between samples above what is tolerable.


HortScience ◽  
2008 ◽  
Vol 43 (5) ◽  
pp. 1544-1548 ◽  
Author(s):  
Ibrahim Demir ◽  
Kazim Mavi

The laboratory germination (radicle emergence) percentages of 9 watermelon, 12 melon, and 7 cucumber seed lots were tested after storing in relatively adverse storage conditions of 25 °C and 12% mc for 6, 12, and 18 months in sealed aluminum foil packets. The laboratory germination (radicle emergence) of lots was determined after controlled deterioration (CD) at 45 °C with 20% or 24% moisture content (mc) for 48, 72, 96, 120, and 144 h. The accelerated aging test (AA) was conducted at 45 °C for the same aging periods. A number of seed lots was dead by 12 and 18 months in watermelon and cucumber, respectively. Various combinations of test regimens were found to be correlated with laboratory germination after 6 months storage, but the most consistent regimens for AA tests was 96 h at 45 °C in all species (r = 0.71 to 0.98). In the CD tests, 72 h with 20% mc at 45 °C gave the best correlation (r = 0.86 to 0.96). These conditions of highest correlation were observed after laboratory germination after 6 months storage and are suggested as good predictors of storage life in cucurbit seed lots. The initial standard germination before storage was also significantly correlated with seed longevity, but the correlation coefficient was generally lower (r = 0.60 to 0.83) than the AA and CD test results and separation of lots less clear.


Sign in / Sign up

Export Citation Format

Share Document