Response of Australian barley cultivars to leaf rust (Puccinia hordei)

1994 ◽  
Vol 34 (6) ◽  
pp. 783 ◽  
Author(s):  
PJ Cotterill ◽  
RG Rees ◽  
GJ Platz
Keyword(s):  
Leaf Rust ◽  
Puccinia Hordei ◽  
Barley Cultivars ◽  
Commercial Cultivars ◽  
Rust Pathogen ◽  
Infection Types ◽  
Moderately Resistant ◽  
Adult Plants

Thirty-eight Australian commercial cultivars of barley were screened as seedlings against 11 pathotypes of the leaf rust pathogen (Puccinia hordei Otth.) detected in Australia, and as adult plants in the field against the 2 predominant pathotypes (210 P+ and 200 P+) present in the eastern mainland states. Most cultivars were susceptible as seedlings to 10 of the pathotypes of P hordei (with infection types of 3 or 4 on a 0-4 scale) but only 17 were susceptible to pathotype 253 P-. Abyssinian, Franklin, Tallon and Triumph were susceptible to none, Skiff only to 210 P+, Grimmett and Shannon only to 210 P+ and 253 P- and Ulandra to pathotypes 202 P+, 243 P-, 243 P+, 253 and 262 P+. Cutter, Ketch, Prior, Schooner and Weeah were susceptible to all except 200 P-, 201 P-, 243 P-, and 253 P-, and Yerong to all except 200 P-, 201 P-, 202 Pt, 220 P+ and 262 P+. Some cultivars, notably Malebo, but also Corvette, Galleon, Schooner and, to a lesser extent, Yerong were not as susceptible (infection types of 3 to 3+/4-) to most pathotypes of P. hordei. As adult plants in the field, Abyssinian, Franklin, Tallon and Triumph were resistant, and Malebo and Ulandra moderately resistant/moderately susceptible to both pathotypes tested.

Tolerance of spring barley cultivars to leaf rust, Puccinia hordei

Euphytica ◽  
1980 ◽  
Vol 29 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Th. Kramer ◽  
B. H. Gildemacher ◽  
M. Van der Ster ◽  
J. E. Parlevliet
Keyword(s):  
Leaf Rust ◽  
Spring Barley ◽  
Puccinia Hordei ◽  
Barley Cultivars

Origin of leaf rust adult plant resistance gene Rph20 in barley

Genome ◽  
2012 ◽  
Vol 55 (5) ◽  
pp. 396-399 ◽  
Author(s):  
Lee T. Hickey ◽  
Wendy Lawson ◽  
Greg J. Platz ◽  
Mark Dieters ◽  
Jerome Franckowiak
Keyword(s):  
Hordeum Vulgare ◽  
Leaf Rust ◽  
Plant Resistance ◽  
Dna Analysis ◽  
Adult Plant Resistance ◽  
Puccinia Hordei ◽  
Adult Plant ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Alternative Sources

Rph20 is the only reported, simply inherited gene conferring moderate to high levels of adult plant resistance (APR) to leaf rust ( Puccinia hordei Otth) in barley ( Hordeum vulgare L.). Key parental genotypes were examined to determine the origin of Rph20 in two-rowed barley. The Dutch cultivar ‘Vada’ (released in the 1950s) and parents, ‘Hordeum laevigatum’ and ‘Gull’ (‘Gold’), along with the related cultivar ‘Emir’ (a derivative of ‘Delta’), were assessed for APR to P. hordei in a disease screening nursery. The marker bPb-0837-PCR, co-located with Rph20 on the short arm of chromosome 5H (5HS), was used to screen genotypes for the resistance allele, Rph20.ai. Results from phenotypic assessment and DNA analysis confirmed that Rph20 originated from the landrace ‘H. laevigatum’ (i.e., Hordeum vulgare subsp. vulgare). Tracing back this gene through the pedigrees of two-rowed barley cultivars, indicated that Rph20 has contributed APR to P. hordei for more than 60 years. Although there have been no reports of an Rph20-virulent pathotype, the search for alternative sources of APR should continue to avoid widespread reliance upon a single resistance factor.

scholarly journals First Detection of Puccinia hordei Virulence to Barley Leaf Rust Resistance Gene Rph3 and Combination with Virulence to Rph7 in North America

Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 838-838 ◽  
Author(s):  
M. N. Rouse ◽  
C. A. Griffey ◽  
W. S. Brooks
Keyword(s):  
North America ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Infection Types ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Barley leaf rust, caused by Puccinia hordei Otth., has been problematic in United States barley (Hordeum vulgare L.) production in the Mid-Atlantic coast region and California. During the early 1990s, P. hordei pathotypes with virulence to resistance gene Rph7 caused average yield losses from 6 to 16% (3). ‘Doyce’ barley was released in 2003 and was described as being resistant to leaf rust (2). Initially in April 2010 and subsequently in spring 2011 and 2012, high severities and infection responses were observed on experimental plots of ‘Doyce’ in Warsaw and Blacksburg, Virginia. Three single uredinial isolates of P. hordei were derived from collections made from ‘Doyce’ barley. The isolates were characterized for virulence to barley leaf rust resistance genes by inoculating at least two replicates of a barley leaf rust differential set including 12 Rph genes (1). Previous methods used for inoculation, incubation, and pathotyping were followed (1). Infection types were scored on a 0 to 4 scale where 2 and below indicated resistance and 3 and above indicated susceptibility (4). The three isolates collected from Doyce barley displayed large pustules with infection types 3,3+ to cultivars Estate (Rph3) and Cebada Capa (Rph7). Avirulent isolates of P. hordei displayed infection types 0; to 0;1c to Estate and ;n to 0;1n to Cebada Capa (1). The data indicated that all three isolates were virulent to both barley leaf rust resistance genes Rph3 and Rph7. Though combined Rph3 and Rph7 virulence has been reported in the Mediterranean region, this is the first report of Rph3 virulence in North America. These isolates of P. hordei are virulent to important sources of resistance to barley leaf rust and threaten barley production in environments conducive for disease development in North America. References: (1) W. S. Brooks et al. Phytopathology 90:1131, 2000. (2) W. S. Brooks et al. Crop Sci. 45:792, 2005. (3) C. A. Griffey et al. Plant Dis. 78:256, 1994. (4) M. N. Levine and W. J. Cherewick. U.S. Dept. Agric. Tech. Bull. 1056, 1952.

scholarly journals Virulence in Puccinia recondita f. sp. tritici Isolates from Canada to Genes for Adult-Plant Resistance to Wheat Leaf Rust

Plant Disease ◽  
1997 ◽  
Vol 81 (3) ◽  
pp. 267-271 ◽  
Author(s):  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Infection Type ◽  
Leaf Rust Resistance ◽  
Seedling Resistance ◽  
High Infection ◽  
Rust Severity ◽  
Lower Infection ◽  
Infection Types ◽  
Adult Plants

Sixty-seven isolates of Puccinia recondita f. sp. tritici collected in Canada were tested for virulence to adult plants of Thatcher near-isogenic wheat lines with leaf rust resistance genes Lr13, Lr22a, Lr34, Lr35, and Lr13 and Lr34 combined. All of the isolates had low infection type to the Thatcher lines with Lr22a and Lr35. All isolates had lower infection type and lower rust severity on the Thatcher line with Lr34 compared with Thatcher. The isolates were polymorphic for virulence to the Thatcher line with Lr13; many isolates were completely virulent to this line, and other isolates produced very low or intermediate avirulent infection type. On the Thatcher line with Lr13 and Lr34 combined, many isolates had infection type and rust severity similar to the Thatcher line with Lr34, while other isolates had lower infection type and rust severity compared with the single-gene lines with Lr13 or Lr34. Fifteen isolates with low, intermediate, and high infection type to adult plants with Lr13 were tested for infection type on seedling plants of the Thatcher lines with resistance genes Lr13, Lr22a, Lr34, Lr35, and Lr37, and on Thatcher lines with Lr13 paired with seedling resistance genes. Most isolates were completely virulent to seedling plants with Lr13 and Lr22a. Plants with Lr37 expressed seedling resistance to all isolates tested. Seedling plants with Lr34 had lower infection types to all isolates compared with Thatcher. Seedlings with Lr35 had high infection types to most isolates, with varying amounts of chlorosis. The Thatcher lines with Lr13 plus seedling resistance genes were most resistant to the isolates that had very low infection types on adult plants with Lr13 and intermediate infection types on plants with seedling resistance genes. Genes Lr22a, Lr35, and Lr37 offer additional sources of highly effective leaf rust resistance in wheat.

scholarly journals Characterization of Rph24: A Gene Conferring Adult Plant Resistance to Puccinia hordei in Barley

2017 ◽  
Vol 107 (7) ◽  
pp. 834-841 ◽  
Author(s):  
Laura A. Ziems ◽  
Lee T. Hickey ◽  
Gregory J. Platz ◽  
Jerome D. Franckowiak ◽  
Peter M. Dracatos ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Genotyping By Sequencing ◽  
Puccinia Hordei ◽  
Adult Plant ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Trait Locus ◽  
Marker Frequency

We identified Rph24 as a locus in barley (Hordeum vulgare L.) controlling adult plant resistance (APR) to leaf rust, caused by Puccinia hordei. The locus was previously reported as a quantitative trait locus in barley line ND24260-1 and named qRphND. We crossed ND24260-1 to the leaf-rust-susceptible standard Gus and determined inheritance patterns in the progeny. For the comparative marker frequency analysis (MFA), resistant and susceptible tails of the F2 were genotyped with Diversity Arrays Technology genotyping-by-sequencing (DArT-Seq) markers. The Rph24 locus was positioned at 55.5 centimorgans on chromosome 6H on the DArT-Seq consensus map. Evaluation of F2:3 families confirmed that a single locus from ND24260-1 conferred partial resistance. The haploblock strongly associated with the Rph24 locus was used to estimate the allele frequency in a collection of 282 international barley cultivars. Rph24 was frequently paired with APR locus Rph20 in cultivars displaying high levels of APR to leaf rust. The markers identified in this study for Rph24 should be useful for marker-assisted selection.

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