Successful stem cutting propagation of chickpea, its wild relatives and their interspecific hybrids

2006 ◽  
Vol 46 (10) ◽  
pp. 1349 ◽  
Author(s):  
N. Danehloueipour ◽  
G. Yan ◽  
H. J. Clarke ◽  
K. H. M. Siddique

A successful stem cutting method was developed to propagate chickpea (Cicer arietinum L.), its crossable wild annual relatives (C. reticulatum Ladiz. and C. echinospermum P.H. Davis) and their interspecific hybrids. The effect of plant growth regulator powder (0.5 mg/g indole butyric acid and 0.5 mg/g naphthalene acetic acid), honey, combined honey + plant growth regulator powder, different growth stages of the donor plant, and rooting substrates on rooting rate, root number, root length, and survival rate were investigated. The highest propagation success rate was achieved when cuttings were taken at the pre-flowering stage, treated with plant growth regulator powder and grown in a sand + potting mix substrate. The rooting rate ranged from 87.5 to 100% for chickpea, C. reticulatum and C. echinospermum, and interspecific hybrids. All of the accessions examined in the study were successfully propagated with the new method. This study provides a simple and efficient technique for vegetative propagation of Cicer species which will be useful for the multiplication of seed, production of clones for disease screening or for the development of mapping populations.

1996 ◽  
Vol 44 (3) ◽  
pp. 343 ◽  
Author(s):  
PM Magdalita ◽  
SW Adkins ◽  
ID Godwin ◽  
RA Drew

An improved embryo-rescue protocol was developed for embryos (90 days old) of Carica papaya L. (Clone 2001), and subsequently was utilised for efficient production of interspecific hybrids of C. papaya × C. cauliflora Jacq. from 90- to 120-day-old embryos. Pre-incubation of C. papaya embryos for 7 days on a germination medium containing half-strength De Fossard nutrients supplemented with gibberellic acid (10 μM), 6-benzylamino-purine (0.25 μM), alpha-naphthalene-acetic acid (0.25 μM). sucrose (58 mM) and agar (8 g L-1) supported 100% germination. Subsequent transfer of germinated embryos to a nutrient medium that was identical, except that it was free of plant growth regulator, allowed good growth but induced shoot etiolation and callus production. Reducing the pre-incubation of C. papaya embryos on this medium to 5 days before transfer to the medium free of plant growth regulator produced similarly high germination (96%), but allowed for the production of good quality seedlings that were unetiolated and free of unwanted callus. For interspecific hybrids, a 5-day pre-incubation of the embryos on a liquid formulation was better than the solid formulation as it promoted better growth and vigour of the normally abortive interspecific hybrid embryos. Using the improved protocol, 1981 of 2100 (94%) interspecific hybrid embryos consisting of single and multiple forms were germinated. In all cases, the germinating multiple embryos underwent further embryogenesis that allowed for the production of 485 (25%) morphologically normal hybrid plants grown in soil in the glasshouse.


2004 ◽  
Vol 31 (2) ◽  
pp. 70-73 ◽  
Author(s):  
J. P. Beasley ◽  
W. J. Grichar ◽  
D. L. Jordan ◽  
R. G. Lemon ◽  
B. A. Besler ◽  
...  

Abstract Experiments were conducted from 1996 through 2001 in Georgia, North Carolina, and Texas to determine peanut response to the commercial plant growth regulator Early Harvest® (a commercial mixture of cytokinin, gibberellic acid, and indole butyric acid). Early Harvest (applied in the seed furrow) or Early Harvest TST (applied as a dry seed treatment) followed by four foliar applications (four-leaf peanut, initial pegging, 14 d after initial pegging, and pod fill), was compared to non-treated peanut in five, 13, and three experiments in these respective states. Early Harvest did not affect pod yield or gross economic value of peanut regardless of location, cultivar, or edaphic and environmental conditions. These data suggest that a spray program consisting of Early Harvest most likely will not improve pod yield or gross economic value of peanut.


1988 ◽  
Vol 2 (4) ◽  
pp. 473-480 ◽  
Author(s):  
Richard H. Hodgson ◽  
Lee A. Wymore ◽  
Alan K. Watson ◽  
Robert H. Snyder ◽  
Anne Collette

The plant pathogenic fungusColletotrichum coccodes(Cc) and the plant growth regulator thidiazuron (TDZ) were evaluated in Maryland and Quebec for velvetleaf control in ‘Williams' and ‘Maple Arrow’ soybean. TDZ was applied at 0, 0.1, 0.2, 0.3, and 0.4 kg ai/ha alone or was combined with Cc at 109spores/m2when velvetleaf was at the 1- to 2-leaf (Trial 1) or 4- to 6-leaf (Trial 2) growth stages. Velvetleaf control increased with TDZ rate, and TDZ combined with Cc further increased control. TDZ reduced velvetleaf biomass and height, and Cc increased velvetleaf mortality. In Quebec, Cc also reduced the biomass of velvetleaf treated in Trial 1 and interacted positively with TDZ at this growth stage. Cc nearly halved the rates of TDZ required for 90 and 75% mortality of velvetleaf treated at the 1- to 2-leaf and 4- to 6-leaf stages to 0.09 and 0.12 kg/ha, respectively, in Quebec. Cc similarly lowered the rate of TDZ required for 75% stand reduction of velvetleaf in Trial 1 to 0.17 kg/ha in Maryland. Cool wet weather in Quebec contrasted with warm, dry weather in Maryland. Soybean biomass and yield were increased significantly by treatment with TDZ plus Cc in Trial 1 at both locations.


Weed Science ◽  
2019 ◽  
Vol 67 (3) ◽  
pp. 346-353
Author(s):  
Brian Van de Stroet ◽  
Graig Reicks ◽  
Deepak Joshi ◽  
Sen Subramanian ◽  
David Clay ◽  
...  

AbstractThe success of dicamba-tolerant soybean [Glycine max(L.) Merr.] has revived concerns about plant growth regulator (PGR) herbicide exposure to conventional soybean. In laboratory studies, soybean root nodulation is inhibited by excess auxin, which is the mechanism of action of PGR herbicides. Soybean exposed to PGRs in a field environment may have a similar response, and if nodulation is compromised, nitrogen (N) fixation may be reduced, with subsequent seed yield or protein content decreases. Many soybean–N studies report minimal impact to soybean yield. However, if soybeans show foliar PGR injury symptoms, could N application compensate for a potential nodulation inhibition response? This study examined the response of non–PGR tolerant soybean to N after exposure to low doses of 2,4-D and dicamba applied once (at soybean growth stages V1, V3, and early reproduction [R1 or R2]) or twice (V1 + V3 or V3 + R). N was either foliar or soil applied at early (∼5 d after PGR application) or late (10 d after PGR application) timings. Nodulation and plant growth were evaluated at R3, and grain yield and seed protein and oil content were quantified at maturity. Plant biomass and nodulation were reduced by 10% with some PGR treatments, and early foliar N application after PGR injury resulted in reduction up to 25%. N applications to non–PGR treated soybean did not increase yield. Soybean treated with PGR at V1 or V3, with or without N, had yields similar to control treatments. However, yield reductions of up to 20% were observed when PGRs were applied at V5 or R stages or when double PGR applications were followed by early foliar N application. Seed protein and oil content were not affected by PGR or N treatment.


2007 ◽  
Vol 35 (2) ◽  
pp. 993-996 ◽  
Author(s):  
Sulejman Redžepović ◽  
Sanja Sikora ◽  
Josip Čolo ◽  
Mihaela Blažinkov ◽  
Marija Pecina

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