Identification of genes related to the regulation of anther and pollen development in Mu-type cytoplasmic male sterile wheat (Triticum aestivum) by transcriptome analysis

2019 ◽  
Vol 70 (4) ◽  
pp. 306 ◽  
Author(s):  
Wei Li ◽  
Zihan Liu ◽  
Chang Meng ◽  
Yulin Jia ◽  
Lingli Zhang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Wheat Breeding ◽  
Abnormal Development ◽  
Nutrient Deficiencies ◽  
Hybrid Wheat ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Pectin Degradation ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Cytoplasmic male sterile (CMS) lines are important tools for hybrid production but they cannot produce viable pollen. Breeding new CMS lines and studying their sterility mechanism in wheat (Triticum aestivum L.) greatly facilitates the process of hybrid wheat breeding. We conducted transcriptome sequencing for a recently identified Mu-CMS line with Aegilops uniaristata Vis. cytoplasm, named U706A, and its isonuclear maintainer line (706B) at the binucleate stage, which was a critical period when abortion occurred. We found that most of the genes involved in phosphatidylinositol metabolism and pectin degradation were downregulated, as well as genes encoding the MYB21 and MYC2 transcription factors, in U706A compared with 706B. In addition, pectin contents indicated that the production of pectin has been enhanced from the binucleate stage to the trinucleate stage, owing to the downregulation of pectin-degradation-related genes in U706A at the binucleate stage, which confirmed the reliability of the sequencing results. We also discovered that the accumulation period of pectin content in U706A is abnormal compared with 706B, which may be an important reason for abortion. Some differentially expressed genes that might be related to the sterile phenotype were verified by quantitative RT-PCR. Therefore, we suggest that the downregulation of these genes possibly leads to the anther not to crack; the tapetum and microspore membrane system is less metabolised, and the abnormal pectin accumulation results in microspore nutrient deficiencies and abnormal development. These findings provide novel insights into the mechanism responsible for pollen abortion in CMS, which may facilitate hybrid wheat breeding.

scholarly journals Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinya Toriyama ◽  
Tomohiko Kazama ◽  
Tadashi Sato ◽  
Yoshimichi Fukuta ◽  
Masaaki Oka
Keyword(s):  
Nuclear Gene ◽  
Oryza Rufipogon ◽  
Rice Cultivars ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Abstract Background A cytoplasm of CW-type cytoplasmic male sterile (CMS) line is derived from Oryza rufipogon strain W1 and fertility is restored by a single nuclear gene, Rf17. We have previously reported that CW-CMS were effective for breeding CMS lines of Indica Group rice cultivars, IR 24 and IR 64. The applicability of this CW-CMS/Rf17 system to produce other elite Indica Group rice cultivars with CMS was explored. Findings Out of seven elite Indica Group rice cultivars, complete CMS lines were obtained for six cultivars: NSIC Rc 160, NSIC Rc 240, Ciherang, BRRI dhan 29, NERICA-L-19, and Pusa Basmati. The fertility of these six lines was restored when Rf17 was present. A CMS line was not obtained for the cultivar Samba Mahsuri. Conclusions The CW-CMS/Rf17 system will be useful to produce CMS lines and restorer lines of various elite Indica Group rice cultivars.

Male fertility restoration of wheat in Hordeum chilense cytoplasm is associated with 6HchS chromosome addition

2008 ◽  
Vol 59 (3) ◽  
pp. 206 ◽  
Author(s):  
A. C. Martín ◽  
S. G. Atienza ◽  
M. C. Ramírez ◽  
F. Barro ◽  
A. Martín
Keyword(s):  
Bread Wheat ◽  
Fertility Restoration ◽  
Triticum Aestivum L ◽  
Abnormal Development ◽  
Hordeum Chilense ◽  
Alloplasmic Line ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Reduced Height ◽  
Pollen Stage

We report a new cytoplasmic male sterility (CMS) source in bread wheat (Triticum aestivum L.) designated as msH1. CMS has been identified during the process of obtaining alloplasmic bread wheat in different Hordeum chilense Roem. Schultz. cytoplasms. It was observed that when using the H. chilense H1 accession, the corresponding alloplasmic line was male sterile. This alloplasmic wheat is stable under different environmental conditions and it does not exhibit developmental or floral abnormalities, showing only slightly reduced height and some delay in heading. On examining microsporogenesis in the alloplasmic line, it was found that different stages of meiosis were completed normally, but abnormal development occurred at the uninucleate-pollen stage at the first mitosis, resulting in failure of anther exertion and pollen abortion. Fertility restoration of the CMS phenotype caused by the H. chilense cytoplasm was associated with the addition of chromosome 6HchS from H. chilense accession H1. Thus, some fertility restoration genes appear to be located in this chromosome arm. Considering the features displayed by the msH1 system, we consider that it has a great potential for the development of viable technology for hybrid wheat production.

THE CYTOLOGY OF POLLEN ABORTION IN S CYTOPLASMIC MALE-STERILE CORN ANTHERS

1980 ◽  
Vol 67 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Sheu-Ling Janet Lee ◽  
E. D. Earle ◽  
V. E. Gracen
Keyword(s):  
Male Sterile ◽  
Pollen Abortion ◽  
Cytoplasmic Male Sterile

scholarly journals The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

2019 ◽  
Vol 20 (24) ◽  
pp. 6252 ◽  
Author(s):  
Junchang Li ◽  
Jing Zhang ◽  
Huijuan Li ◽  
Hao Niu ◽  
Qiaoqiao Xu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Male Sterility ◽  
Genetic Research ◽  
Triticum Aestivum L ◽  
Anther Development ◽  
Sucrose Metabolism ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Microspore Stage ◽  
Regulation Model

Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.

scholarly journals Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding

2020 ◽  
Author(s):  
Hongsheng Li ◽  
Shaoxiang Li ◽  
Sedhom Abdelkhalik ◽  
Armaghan Shahzad ◽  
Jian Gu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Doubled Haploid ◽  
Yellow Rust ◽  
Wheat Breeding ◽  
Yield Potential ◽  
Limiting Factor ◽  
Hybrid Wheat ◽  
Male Sterile ◽  
Dh Lines ◽  
F2 Generation

Abstract Background: Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past twenty years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids. Application of doubled haploid (DH) breeding provides a useful strategy to efficiently develop practically usable TPSGMS lines. Results: F1s and selected F2 and F3 sterile plants of eight crosses made from two commercial TPSGMS lines were used to produce DH lines. We developed a total of 24 elite DH sterile lines with stable sterility, good outcrossing and yield potential, resistance to yellow rust and powdery mildew, as well as desirable plant height (50-60 cm). These DH lines were developed within 4 years through at least one year of evaluation. The stability of male sterility was confirmed for most (20/24) of these elite DH sterile lines by multiple tests in two or three years. These lines are expected to be used in hybrid wheat breeding. The percentage of elite lines developed from the tested DH lines produced from filial generations was in the order of F2 > F3 > F1. Conclusions: We demonstrate that coupling DH techniques with conventional breeding is an efficient strategy for accelerating the development of more practical wheat TPSGMS lines. Generation of DHs from F2 generation appeared to be the better choice considering the balance of shortening breeding time and overall breeding efficiency.

THE CYTOLOGY OF POLLEN ABORTION IN C-CYTOPLASMIC MALE-STERILE CORN ANTHERS

1979 ◽  
Vol 66 (6) ◽  
pp. 656-667 ◽  
Author(s):  
Sheu-Ling Janet Lee ◽  
V. E. Gracen ◽  
E. D. Earle
Keyword(s):  
Male Sterile ◽  
Pollen Abortion ◽  
Cytoplasmic Male Sterile
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