DNA methylation of the TAA1 gene regulates formation of the pollen wall in chemically induced male sterility in wheat (Triticum aestivum)

2017 ◽  
Vol 68 (9) ◽  
pp. 817
Author(s):  
Guiping Li ◽  
Qingsong Ba ◽  
Gaisheng Zhang ◽  
Lanlan Zhang ◽  
Chu Chen ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Triticum Aestivum ◽  
Male Sterility ◽  
Epigenetic Modification ◽  
Core Promoter ◽  
Pollen Wall ◽  
Pcr Analysis ◽  
Tetradecanoic Acid ◽  
Expression Levels ◽  
Chemically Induced

DNA methylation is an important epigenetic modification that may contribute to environmentally induced phenotypic variations by regulating gene expression. Chemically induced male sterility (CIMS) lines in wheat (Triticum aestivum L.) can transform from sterile to fertile, induced by a chemical hybridising agent during anther development. So far, little is known about the DNA methylation variation of CIMS in wheat. TAA1 regulates pollen wall development, probably through converting fatty acids to fatty alcohol in wheat. We investigated the DNA methylation pattern of the TAA1 gene in the core promoter region by using the bisulfite genomic sequencing method, and higher methylation was observed in CIMS. The expression levels of the TAA1 gene were also evaluated by real time quantitative reverse transcriptase PCR analysis, which revealed that the expression levels of the TAA1 gene were downregulated in CIMS. The aliphatic composition of the anther underwent accumulation in line 1376-CIMS, revealed by gas chromatography–mass spectrometry, including increments of tetradecanoic acid, hexadecanoic acid and octadecanoic acid. Scanning electron microscopy revealed that anther and pollen wall formation was significantly altered in 1376-CIMS.These results suggested that DNA methylation of the TAA1 gene may be involved in the sterility–fertility transition of CIMS.

Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.)

2013 ◽  
Vol 36 (2) ◽  
pp. 503-512 ◽  
Author(s):  
Qingsong Ba ◽  
Gaisheng Zhang ◽  
Junsheng Wang ◽  
Na Niu ◽  
Shoucai Ma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Triticum Aestivum ◽  
Male Sterility ◽  
Triticum Aestivum L ◽  
Chemically Induced

Phenotype characterisation and analysis of expression patterns of genes related mainly to carbohydrate metabolism and sporopollenin in male-sterile anthers induced by high temperature in wheat (Triticum aestivum)

2018 ◽  
Vol 69 (5) ◽  
pp. 469 ◽  
Author(s):  
Hongzhan Liu ◽  
Junsheng Wang ◽  
Chaoqiong Li ◽  
Lin Qiao ◽  
Xueqin Wang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
High Temperature ◽  
Male Sterility ◽  
Carbohydrate Metabolism ◽  
Higher Plants ◽  
Expression Patterns ◽  
Triticum Aestivum L ◽  
Male Sterile ◽  
Tetrad Stage ◽  
Expression Levels

Male reproductive development in higher plants is highly sensitive to various stressors, including high temperature (HT). In this study, physiological male-sterile plants of wheat (Triticum aestivum L.) were established using HT induction. The physiological changes and expression levels of genes mainly related to carbohydrate metabolism and sporopollenin in male-sterile processes were studied by using biological techniques, including iodine–potassium iodide staining, paraffin sectioning, scanning electron microscopy (SEM) and fluorescent quantitative analysis. Results of paraffin sectioning and SEM revealed that parts of HT male-sterile anthers, including the epidermis and tapetum, were remarkably different from those of normal anthers. The expression levels of TaSUT1, TaSUT2, IVR1 and IVR5 were significantly lower than of normal anthers at the early microspore and trinucleate stages. The RAFTIN1 and TaMS26 genes may contribute to biosynthesis and proper ‘fixation’ of sporopollenin in the development of pollen wall; however, their expression levels were significantly higher at the early tetrad stage and early microspore stage in HT sterile anthers. The recently cloned MS1 gene was expressed at the early tetrad and early microspore stages but not at the trinucleate stage. Moreover, this gene showed extremely significant, high expression in HT sterile anthers compared with normal anthers. These results demonstrate that HT induction of wheat male sterility is probably related to the expression of genes related to carbohydrate metabolism and sporopollenin metabolism. This provides a theoretical basis and technological approach for further studies on the mechanisms of HT induction of male sterility.

scholarly journals Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

2018 ◽  
Vol 19 (9) ◽  
pp. 2689 ◽  
Author(s):  
Zhixin Wang ◽  
Xiangping Wu ◽  
Zengxiang Wu ◽  
Hong An ◽  
Bin Yi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Male Sterility ◽  
Bisulfite Sequencing ◽  
Epigenetic Modification ◽  
Differentially Methylated Region ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Floral Buds ◽  
Global Comparison ◽  
Genome Wide

DNA methylation is an essential epigenetic modification that dynamically regulates gene expression during plant development. However, few studies have determined the DNA methylation profiles of male-sterile rapeseed. Here, we conducted a global comparison of DNA methylation patterns between the rapeseed genic male sterile line 7365A and its near-isogenic fertile line 7365B by whole-genome bisulfite sequencing (WGBS). Profiling of the genome-wide DNA methylation showed that the methylation level in floral buds was lower than that in leaves and roots. Besides, a total of 410 differentially methylated region-associated genes (DMGs) were identified in 7365A relative to 7365B. Traditional bisulfite sequencing polymerase chain reaction (PCR) was performed to validate the WGBS data. Eleven DMGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. In particular, Bnams4 was hypo-methylated in 7365A, and its expression was up-regulated, which might affect other DMGs and thus control the male sterility. This study provided genome-wide DNA methylation profiles of floral buds and important clues for revealing the molecular mechanism of genic male sterility in rapeseed.

scholarly journals Effects of cytochalasin B on DNA methylation and histone modification in parthenogenetically activated porcine embryos

Reproduction ◽  
2016 ◽  
Vol 152 (5) ◽  
pp. 519-527 ◽  
Author(s):  
Xiaoxiao Hou ◽  
Jun Liu ◽  
Zhiren Zhang ◽  
Yanhui Zhai ◽  
Yutian Wang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Embryonic Development ◽  
Histone Modification ◽  
Actin Polymerization ◽  
Cytochalasin B ◽  
Epigenetic Modification ◽  
Cell Activity ◽  
Cleavage Rate ◽  
Expression Levels ◽  
Mammalian Embryos

DNA methylation and histone modification play important roles in the development of mammalian embryos. Cytochalasin B (CB) is an actin polymerization inhibitor that can significantly affect cell activity and is often used in studies concerning cytology. In recent years, CB is also commonly being used inin vitroexperiments on mammalian embryos, but few studies have addressed the effect of CB on the epigenetic modification of embryonic development, and the mechanism underlying this process is also unknown. This study was conducted to investigate the effects of CB on DNA methylation and histone modification in the development of parthenogenetically activated porcine embryos. Treatment with 5 μg/mL CB for 4 h significantly increased the cleavage rate, blastocyst rate and total cell number of blastocysts. However, the percentage of apoptotic cells and the expression levels of the apoptosis-related genesBCL-XL,BAXandCASP3were significantly decreased. Treatment with CB significantly decreased the expression levels ofDNMT1,DNMT3a,DNMT3b,HAT1andHDAC1at the pronuclear stage and promoted the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). After CB treatment, the level of AcH3K9 was upregulated and the level of H3K9me3 was downregulated. When combined with Scriptaid and 5-Aza-Cdr, CB further improved the embryonic development competence and decreased the expression ofBCL-XL,BAXandCASP3. In conclusion, these results suggest that CB could improve embryonic development and the quality of the blastocyst by improving the epigenetic modification during the development of parthenogenetically activated embryos.

Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.)

2013 ◽  
Vol 93 (4) ◽  
pp. 675-681 ◽  
Author(s):  
Qing Song Ba ◽  
Gai Sheng Zhang ◽  
Jun Sheng Wang ◽  
Hui Xue Che ◽  
Hong Zhan Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Triticum Aestivum ◽  
Male Sterility ◽  
Reactive Oxygen ◽  
Triticum Aestivum L ◽  
Oxygen Species ◽  
Ros Scavenging ◽  
Male Sterile ◽  
Chemically Induced

Ba, Q. S., Zhang, G. S., Wang, J. S., Che, H. X., Liu, H. Z., Niu, N., Ma, S. C. and Wang, J. W. 2013. Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.). Can. J. Plant Sci. 93: 675–681. Chemically induced male sterility (CIMS) systems in wheat are among the male sterility types used for hybrid wheat (Triticum aestivum L.) production in China. Some studies suggested that male sterile line Xi'nong 1376-CIMS induced by chemical hybridizing agents (CHA) may suffer from oxidative stress as its cyanide-resistant respiration is lower than that of Xi'nong1376. To elucidate the metabolic mechanism of reactive oxygen species (ROS) in the CIMS anthers, the metabolism changes in the production and scavenging of ROS and gene expression related to ROS-scavenging enzymes were investigated in the anther of Xi'nong 1376-CIMS and Xi'nong1376.Anthers of Xi'nong 1376-CIMS had higher contents of [Formula: see text] and H2O2 than those of 1376, which corresponds to expression level of the NADPH oxidase (NOX) gene, and has higher contents of malondialdehyde compared with 1376. Simultaneously, there were lower activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascrodate peroxidase (APX) in scavenging ROS in the anthers of the Xi'nong 1376-CIMS line than in Xi'nong1376. Meanwhile, the expressions of SOD, POD, CAT and APX genes in 1376 were always higher at different levels than those in the Xi'nong 1376-CIMS line except for POD in stage 1. Therefore, it is possible that the sterility in Xi'nong 1376-CIMS is related to the abortion of microspores induced by chronic oxidative stress caused by an abnormal increase in ROS.

scholarly journals Changes in the Expression of DNA Methylation Related Genes in Leukocytes of Persons with Alcohol and Drug Dependence

2020 ◽  
Vol 47 (4) ◽  
pp. 11-17
Author(s):  
M. Krasteva ◽  
Y. Koycheva ◽  
T. Taseva ◽  
S. Simeonova
Keyword(s):  
Dna Methylation ◽  
Mrna Expression ◽  
Drug Addiction ◽  
Drug Dependence ◽  
Transcriptional Activity ◽  
Pcr Analysis ◽  
Disease Manifestation ◽  
Altered Expression ◽  
Expression Levels ◽  
Relative Mrna Expression

AbstractBackground and objectives. Though numerous studies have shown that the dysregulation of the epigenetic control is involved in disease manifestation, limited data is available on the transcriptional activity of DNA methylation related genes in alcohol and drug addiction. With regard to this, in this study we analyzed the expression levels of genes involved in DNA methylation, including DNMT1, DNMT3a, MeCP2, MBD1, MBD2, MBD3 and MBD4, in blood samples of alcohol and drug dependent persons in comparison to healthy abstainers.Methods. The study included 51 participants: 16 persons with alcohol dependence, 17 persons with drug dependence and 18 clinically healthy controls. To detect the relative mRNA expression levels of the studied genes, Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis was applied.Results. Of the seven studied genes, four showed altered expression. MeCP2 and MBD1 were downregulated in the alcohol dependent group (FC = 0.805, p = 0.015 and FC = 0.846, p = 0.034, respectively), while DNMT1 and MBD4 were upregulated in the group with drug dependence (FC = 1.262, p = 0.001 and FC = 1.249, p = 0.005, respectively). No statistically significant changes in the relative mRNA expression were found for DNMT3a, MBD2 and MBD3 genes.Conclusions. Our results are indicative for a role of DNA methylation related genes in alcohol and drug addiction mediated through changes in their transcriptional activity. Studies in this direction will enable better understanding of the underlying mechanisms of addictions supporting the development of more effective therapeutic strategies.

scholarly journals Integrated Methylome and Transcriptome Analysis between the CMS-D2 Line ZBA and Its Maintainer Line ZB in Upland Cotton

2019 ◽  
Vol 20 (23) ◽  
pp. 6070 ◽  
Author(s):  
Meng Zhang ◽  
Liping Guo ◽  
Tingxiang Qi ◽  
Xuexian Zhang ◽  
Huini Tang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Male Sterility ◽  
Upland Cotton ◽  
Epigenetic Modification ◽  
Atp Synthesis ◽  
H2o2 Production ◽  
Maintainer Line ◽  
Differentially Methylated Genes ◽  
Genes Encoding ◽  
Oxidative Phosphorylation Pathway

DNA methylation is an important epigenetic modification involved in multiple biological processes. Altered methylation patterns have been reported to be associated with male sterility in some plants, but their role in cotton cytoplasmic male sterility (CMS) remains unclear. Here, integrated methylome and transcriptome analyses were conducted between the CMS-D2 line ZBA and its near-isogenic maintainer line ZB in upland cotton. More methylated cytosine sites (mCs) and higher methylation levels (MLs) were found among the three sequence contexts in ZB compared to ZBA. A total of 4568 differentially methylated regions (DMRs) and 2096 differentially methylated genes (DMGs) were identified. Among the differentially expressed genes (DEGs) associated with DMRs (DMEGs), 396 genes were upregulated and 281 genes were downregulated. A bioinformatics analysis of these DMEGs showed that hyper-DEGs were significantly enriched in the “oxidative phosphorylation” pathway. Further qRT-PCR validation indicated that these hypermethylated genes (encoding the subunits of mitochondrial electron transport chain (ETC) complexes I and V) were all significantly upregulated in ZB. Our biochemical data revealed a higher extent of H2O2 production but a lower level of adenosine triphosphate (ATP) synthesis in CMS-D2 line ZBA. On the basis of the above results, we propose that disrupted DNA methylation in ZBA may disrupt the homeostasis of reactive oxygen species (ROS) production and ATP synthesis in mitochondria, triggering a burst of ROS that is transferred to the nucleus to initiate programmed cell death (PCD) prematurely, ultimately leading to microspore abortion. This study illustrates the important role of DNA methylation in cotton CMS.

scholarly journals COL4A3 expression in asthmatic epithelium depends on intronic methylation and ZNF263 binding

2021 ◽  
pp. 00802-2020
Author(s):  
Sai Sneha Priya Nemani ◽  
Cornelis Joseph Vermeulen ◽  
Martin Pech ◽  
Alen Faiz ◽  
Brian George G. Oliver ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Rna Sequencing ◽  
Airway Epithelium ◽  
Epigenetic Modification ◽  
Healthy Controls ◽  
Expression Levels ◽  
Asthmatic Airway ◽  
Bronchial Biopsies ◽  
Bead Arrays

BackgroundReduction of COL4A3 in asthmatic airways, one of the six isoforms of collagen 4 results in increased inflammation and angiogenesis implicating it as a central part of asthma pathogenesis. However, the path underlying these diminished COL4A3 levels has been elusive to date. This study investigated a possible mechanism underlying the reduction of COL4A3 expression.MethodsBronchial biopsies of n=76 asthmatics and n=83 controls were subjected to RNA-sequencing and DNA methylation bead arrays to identify expression and methylation changes. The binding of ZNF263 was analysed by ChiP-Seq coupled with qPCR. Effects of ZNF263 silencing, using siRNA, on the COL4A3 expression were studied by qPCR.ResultsCOL4A3 expression was significantly reduced in bronchial biopsies compared to healthy controls whereas DNA methylation levels at cg11797365 were increased. COL4A3 expression levels were significantly low in asthmatics without ICS use whereas the expression was not statistically different between asthmatics using ICS and controls. Methylation levels at cg11797365 in vitro were increased upon consecutive rhinovirus infections.ConclusionOur data indicates an epigenetic modification as a contributing factor for the loss of COL4A3 expression in asthmatic airway epithelium.

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