Identification of drought-inducible regulatory factors in Lablab purpureus by a comparative genomic approach

2018 ◽  
Vol 69 (6) ◽  
pp. 632 ◽  
Author(s):  
Biao Wang ◽  
Mingzhe Zhao ◽  
Luming Yao ◽  
Maldonado dos Santos Joao V ◽  
Valliyodan Babu ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Detailed Examination ◽  
Wrky Transcription Factor ◽  
Mitogen Activated Protein Kinase ◽  
Comparative Genomic ◽  
Functional Domain ◽  
Lablab Purpureus ◽  
Ppi Networks ◽  
Subtractive Hybridisation ◽  
Hyacinth Bean

Physiological indices of two lines of hyacinth bean (Lablab purpureus (L.) Sweet), MEIDOU 2012 and NANHUI 23, were compared during a progressive course of water-withholding treatments. MEIDOU 2012 was shown to be a superior drought-tolerant genotype. A suppressive subtractive hybridisation library was then constructed by using drought-stressed MEIDOU 2012 roots and sequenced to screen for transcripts with differential abundance variations. In total, 2792 unigenes were assembled from 4064 drought-induced expressed sequence tags. Comparative analysis with other legume genomic sequences, including soybean (Glycine max (L.) Merr.), common bean (Phaseolus vulgaris L.) and lucerne (Medicago sativa L.), was performed, from which 338 unigenes associated with root growth and drought response were identified. Among these unigenes, 27 transcription factors were deduced by functional-domain prediction. The transcription levels of a GRAS and a WRKY transcription factor were confirmed to be responding sensitively to water stresses. Additionally, protein–protein interaction (PPI) networks were predicted for all drought-induced root-related unigenes with different stringency levels. A mitogen-activated protein kinase (MAPK4) and an EF-hand calcium-binding protein (CML24) module were pinpointed to be putative ‘master’ signalling hubs in these PPI networks. Detailed examination of these genomic resources would further reveal key regulators of Lablab drought tolerance.

scholarly journals Variations in Growth and Yield of Indigenous Hyacinth Bean (Lablab purpureus (L.) Sweet) Genotypes

2015 ◽  
Vol 12 (2) ◽  
pp. 01-05
Author(s):  
H Barua ◽  
M H Rahman ◽  
M M Alam Patwary ◽  
M Zahirul Alam ◽  
S Nahar
Keyword(s):  
Agricultural Research ◽  
Minimum Weight ◽  
Growth And Yield ◽  
Research Station ◽  
Lablab Purpureus ◽  
Maximum Weight ◽  
Agricultural Research Institute ◽  
Green Seed ◽  
Minimum Number ◽  
Hyacinth Bean

Variations in growth and yield of three hyacinth bean genotypes collected from Sitakundu of Chittagong, were investigated at the Agricultural Research Station (ARS) of Bangladesh Agricultural Research Institute (BARI), Pahartali, Chittagong during November 2011 to March 2012, where BARI Seem-4 was used as standard control. The minimum number of days for pod formation (54 days) was recorded in BARI Seem-4, while the maximum was in DLP 002 (62 days). The highest number of pods (324) per plant was obtained from DLP 001 followed by DLP 003 (212.33) and the lowest (148.33) was obtained from BARI Seem-4. The maximum weight (12.61 g) of single pod was observed in DLP 003, which is close to that of DLP 002 (12.23 g). The minimum weight of single pod (7.08g) was however observed in DLP 001. Weight of 100-green seed (117.17 g) was maximum in DLP 002, while that of DLP 001 was the minimum (85.90 g).The maximum green seed (61.86 %) was found in DLP 003 and the minimum was in DLP 001 (47.89 %). DLP 003 produced the highest pod yield (26.77 t/ha) which was followed by DLP 001 (22.94 t/ha), while BARI Seem-4 produced the lowest (17.43 t/ha), which is close to that of DLP 002 (19.57 t/ha).The Agriculturists 2014; 12(2) 01-05

scholarly journals Physico-morphological variation in hyacinth bean [Lablab purpureus (L.) Sweet]

1970 ◽  
Vol 35 (3) ◽  
pp. 431-438 ◽  
Author(s):  
MS Islam ◽  
MM Rahman ◽  
T Hossain
Keyword(s):  
Seed Weight ◽  
Lablab Purpureus ◽  
Early Variety ◽  
Leaf Petiole ◽  
Qualitative And Quantitative ◽  
Green Seed ◽  
Pod Yield ◽  
Quantitative Characters ◽  
Physical Traits ◽  
Hyacinth Bean

Forty-four hyacinth bean genotypes were evaluated for different qualitative and quantitative characters during July 2005 to February 2006 at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur. The genotypes showed considerable variations for most of the morpho-physical traits. Shape, size and colour of vein, leaf, petiole, stem, flower, pod and seed varied among the genotypes. Days to first flower ranged from 47.6 to 136.3 days indicating the presence of early variety. Individual pod weight varied from 1.47 (HB042) to 12.3g (HB009). The genotype HB027 produced the maximum number of pods/ plant (425) closely followed by HB001 (385). Similar trend was observed for pod yield/plant. The genotype HB027 produced the highest pod yield/plant (3.45kg) followed by HB001 (3.35kg). 100-green seed weight ranged from 4.0g to 73.33g, which indicated the presence of bold seeded genotypes. Among the genotypes, HB027 and HB007 produced very bold green seed and higher green pod yield/plant, therefore, they can be selected for both pod and green seed production purpose. Keywords: Physico-morphological; hyacinth bean DOI: 10.3329/bjar.v35i3.6450Bangladesh J. Agril. Res. 35(3) : 431-438

scholarly journals In-silico genome wide analysis of Mitogen activated protein kinase kinase kinase gene family in C. sinensis

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0258657
Author(s):  
Abhirup Paul ◽  
Anurag P. Srivastava ◽  
Shreya Subrahmanya ◽  
Guoxin Shen ◽  
Neelam Mishra
Keyword(s):  
Protein Kinase ◽  
Regulatory Elements ◽  
Stress Factors ◽  
Mitogen Activated Protein Kinase ◽  
Functional Study ◽  
Functional Domain ◽  
Genome Wide ◽  
A Genome ◽  
Mitogen Activated Protein ◽  
Protein Kinase Kinase

Mitogen activated protein kinase kinase kinase (MAPKKK) form the upstream component of MAPK cascade. It is well characterized in several plants such as Arabidopsis and rice however the knowledge about MAPKKKs in tea plant is largely unknown. In the present study, MAPKKK genes of tea were obtained through a genome wide search using Arabidopsis thaliana as the reference genome. Among 59 candidate MAPKKK genes in tea, 17 genes were MEKK-like, 31 genes were Raf-like and 11 genes were ZIK- like. Additionally, phylogenetic relationships were established along with structural analysis, which includes gene structure, its location as well as conserved motifs, cis-acting regulatory elements and functional domain signatures that were systematically examined. Also, on the basis of one orthologous gene found between tea and Arabidopsis, functional interaction was carried out in C. sinensis based on an Arabidopsis association model. The expressional profiles indicated major involvement of MAPKKK genes from tea in response to various abiotic stress factors. Taken together, this study provides the targets for additional inclusive identification, functional study, and provides comprehensive knowledge for a better understanding of the MAPKKK cascade regulatory network in C. sinensis.

scholarly journals Callosobruchus phaseoli (Gyllenhal, 1833) (Coleoptera, Chrysomelidae, Bruchinae): a new invasive species in Kazakhstan

2020 ◽  
Vol 6 ◽  
pp. 87-92
Author(s):  
Izbasar I. Temreshev ◽  
Vladimir L. Kazenas
Keyword(s):  
Far East ◽  
Lima Bean ◽  
Pigeon Pea ◽  
Phaseolus Lunatus ◽  
Lablab Purpureus ◽  
The South ◽  
Cowpea Weevil ◽  
Hyacinth Bean ◽  
Beetle Damage ◽  
Callosobruchus Phaseoli

An invasive seed-beetle species cowpea weevil Callosobruchus phaseoli (Gyllenhal, 1833), was found in the south-eastern Kazakhstan (Almaty city) for the first time. Its areal includes India (species origin), South and Central America, Europe, Middle East (Israel), North Africa, Arabian Peninsula, Far East, China, Japan, Sri Lanka, Indonesia, Burma, Philippines, Hawaiian Islands, Australia, and Oceania. Damaged plants are adzuki bean Vigna angularis (Willd.) Ohwi & H. Ohashi (1969), mung bean Vigna radiata (L.) R. Wilczek, broad bean Vicia faba Linnaeus, 1753, pea Pisum sativum Linnaeus, 1753, pigeon pea Cajanus cajan (L.) Huth, 1893, hyacinth bean Lablab purpureus (L.) Sweet, 1826, Wisteria sp., lima bean Phaseolus lunatus Linnaeus, 1753, common bean Phaseolus vulgaris Linnaeus, 1753 and other species of beans, chickpea Cicer arietinum Linnaeus, 1753, Sesbania sp., rattlepod Crotalaria spectabilis Roth., lupine Lupinus sp. Emerged beetles immediately mate and begin to lay eggs on the same day. Beetle damage both in field and in storage. We were not able to find the species during the monitoring of agriculture lands and natural landscapes near the city. We assumed that the invasion occurred recently and the species did not have time to spread outside Almaty. Since C. phaseoli was discovered in Almaty, which is a transit crossroad for many trade routes, further species distribution should be predicted. The most probable corridor for further invasion of cowpea weevil in Kazakhstan is the south and southeast parts of the country, namely Almaty, Zhambyl, Turkestan, and Kyzylorda oblast's.

scholarly journals (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates MPTP-Induced Dopaminergic Neurodegeneration by Inhibiting the STAT3 Pathway

2019 ◽  
Vol 20 (11) ◽  
pp. 2632 ◽  
Author(s):  
Ji Yeon Choi ◽  
Jaesuk Yun ◽  
Chul Ju Hwang ◽  
Hee Pom Lee ◽  
Hae Deun Kim ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Cultured Cells ◽  
Mapk Pathway ◽  
Inflammatory Marker ◽  
Mitogen Activated Protein Kinase ◽  
Dopamine Depletion ◽  
Behavioral Impairment ◽  
Mao B ◽  
Dopaminergic Neurodegeneration ◽  
Anti Inflammatory

Neuroinflammation is implicated in dopaminergic neurodegeneration. We have previously demonstrated that (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol (MMPP), a selective signal transducer and activator of transcription 3 (STAT3) inhibitor, has anti-inflammatory properties in several inflammatory disease models. We investigated whether MMPP could protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic cell loss and behavioral impairment. Imprinting control region (ICR) mice (8 weeks old, n = 10 per group) were administered MMPP (5 mg/kg) in drinking water for 1 month, and injected with MPTP (15 mg/kg, four times with 2 h intervals) during the last 7 days of treatment. MMPP decreased MPTP-induced behavioral impairments in rotarod, pole, and gait tests. We also showed that MMPP ameliorated dopamine depletion in the striatum and inflammatory marker elevation in primary cultured neurons by high-performance liquid chromatography and immunohistochemical analysis. Increased activation of STAT3, p38, and monoamine oxidase B (MAO-B) were observed in the substantia nigra and striatum after MPTP injection, effects that were attenuated by MMPP treatment. Furthermore, MMPP inhibited STAT3 activity and expression of neuroinflammatory proteins, including ionized calcium binding adaptor molecule 1 (Iba1), inducible nitric oxide synthase (iNOS), and glial fibrillary acidic protein (GFAP) in 1-methyl-4-phenylpyridinium (MPP+; 0.5 mM)-treated primary cultured cells. However, mitogen-activated protein kinase (MAPK) inhibitors augmented the activity of MMPP. Collectively, our results suggest that MMPP may be an anti-inflammatory agent that attenuates dopaminergic neurodegeneration and neuroinflammation through MAO-B and MAPK pathway-dependent inhibition of STAT3 activation.

MK2 Is a Therapeutic Target for High-Risk Multiple Myeloma

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5612-5612
Author(s):  
Chunyan Gu ◽  
Hongbao Yang ◽  
Hailin Peng ◽  
Zhiye Xu ◽  
Ruini Chen ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
High Risk ◽  
Therapeutic Target ◽  
Conflicts Of Interest ◽  
Mitogen Activated Protein Kinase ◽  
Comparative Genomic ◽  
Mechanism Study ◽  
Akt Phosphorylation ◽  
Protein Levels

Abstract p38 mitogen-activated protein kinase (MAPK) is constitutively activated in human multiple myeloma (MM). However, a major substrate of p38, MAPKAPK2 (MK2) has received little attention in MM. In this study, we disclosed that elevation of MK2 on DNA, mRNA and protein levels in MM cells compared to normal plasma control cells by array-based comparative genomic hybridization (aCGH), Gene expression profiling (GEP) and immunohistochemistry (IHC) respectively. High MK2 expression prognosticated inferior outcome of newly diagnosed myelomas, particularly in patients with high-risk disease based on GEP. Follow-up study demonstrated that enforced overexpressing MK2 promoted MM cell proliferation, drug-resistance and xenograft tumorigenesis in vivo, while knockdown MK2 expression by shRNA abrogated these characteristics. Further mechanism study showed MK2 directly bound and activated AKT signaling in MM, while specific MK2 inhibitor inhibited MM cell growth and clonogenicity through suppressing AKT phosphorylation. Our findings not only indicate that MK2 is a potential therapeutic target in high-risk MM but also suggest the clinical relevance of MK2 inhibition in MM. Disclosures No relevant conflicts of interest to declare.

scholarly journals Asexual Propagation of a Virulent Clone Complex in a Human and Feline Outbreak of Sporotrichosis

2014 ◽  
Vol 14 (2) ◽  
pp. 158-169 ◽  
Author(s):  
Marcus de Melo Teixeira ◽  
Anderson Messias Rodrigues ◽  
Clement K. M. Tsui ◽  
Luiz Gonzaga Paulo de Almeida ◽  
Anne D. Van Diepeningen ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Mating Type ◽  
Sequence Data ◽  
Fungal Infections ◽  
Haplotype Diversity ◽  
Sporothrix Schenckii ◽  
Mitogen Activated Protein Kinase ◽  
Comparative Genomic ◽  
Asexual Propagation ◽  
Content Type

ABSTRACT Sporotrichosis is one of the most frequent subcutaneous fungal infections in humans and animals caused by members of the plant-associated, dimorphic genus Sporothrix . Three of the four medically important Sporothrix species found in Brazil have been considered asexual as no sexual stage has ever been reported in Sporothrix schenckii , Sporothrix brasiliensis , or Sporothrix globosa . We have identified the mating type ( MAT ) loci in the S. schenckii (strain 1099-18/ATCC MYA-4821) and S. brasiliensis (strain 5110/ATCC MYA-4823) genomes by using comparative genomic approaches to determine the mating type ratio in these pathogen populations. Our analysis revealed the presence of a MAT1-1 locus in S. schenckii while a MAT1-2 locus was found in S. brasiliensis representing genomic synteny to other Sordariomycetes . Furthermore, the components of the mitogen-activated protein kinase (MAPK)-pheromone pathway, pheromone processing enzymes, and meiotic regulators have also been identified in the two pathogens, suggesting the potential for sexual reproduction. The ratio of MAT1-1 to MAT1-2 was not significantly different from 1:1 for all three Sporothrix species, but the population of S. brasiliensis in the outbreaks originated from a single mating type. We also explored the population genetic structure of these pathogens using sequence data of two loci to improve our knowledge of the pattern of geographic distribution, genetic variation, and virulence phenotypes. Population genetics data showed significant population differentiation and clonality with a low level of haplotype diversity in S. brasiliensis isolates from different regions of sporotrichosis outbreaks in Brazil. In contrast, S. schenckii isolates demonstrated a high degree of genetic variability without significant geographic differentiation, indicating the presence of recombination. This study demonstrated that two species causing the same disease have contrasting reproductive strategies and genetic variability patterns.

Regulation of S100A2 expression by TGF-β-induced MEK/ERK signalling and its role in cell migration/invasion

2012 ◽  
Vol 447 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Sarwat Naz ◽  
Prathibha Ranganathan ◽  
Priyanka Bodapati ◽  
Arun H. Shastry ◽  
Laxmi N. Mishra ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cancer Progression ◽  
Calcium Binding ◽  
Transforming Growth Factor ◽  
P53 Protein ◽  
Dominant Negative ◽  
Mitogen Activated Protein Kinase ◽  
Migration And Invasion ◽  
Potential Biomarker ◽  
Tgf Β1

S100A2, an EF hand calcium-binding protein, is a potential biomarker in several cancers and is also a TGF-β (transforming growth factor-β)-regulated gene in melanoma and lung cancer cells. However, the mechanism of S100A2 regulation by TGF-β and its significance in cancer progression remains largely unknown. In the present study we report the mechanism of S100A2 regulation by TGF-β and its possible role in TGF-β-mediated tumour promotion. Characterization of the S100A2 promoter revealed an AP-1 (activator protein-1) element at positions −1161 to −1151 as being the most critical factor for the TGF-β1 response. Chromatin immunoprecipitation and electrophoretic mobility-shift assays confirmed the functional binding of the AP-1 complex, predominantly JunB, to the S100A2 promoter in response to TGF-β1 in HaCaT keratinocytes. JunB overexpression markedly stimulated the S100A2 promoter which was blocked by the dominant–negative JunB and MEK1 [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase 1] inhibitor, PD98059. Intriguingly, despite the presence of a putative SMAD-binding element, S100A2 regulation by TGF-β1 was found to be SMAD3 independent. Interestingly, p53 protein and TGF-β1 show synergistic regulation of the S100A2 promoter. Finally, knockdown of S100A2 expression compromised TGF-β1-induced cell migration and invasion of Hep3B cells. Together our findings highlight an important link between the TGF-β1-induced MAPK and p53 signalling pathways in the regulation of S100A2 expression and pro-tumorigenic actions.

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