Microorganism profile, fermentation quality and rumen digestibility in vitro of maize-stalk silages produced at different maturity stages

2017 ◽  
Vol 68 (3) ◽  
pp. 225 ◽  
Author(s):  
Miao Zhang ◽  
Yanping Wang ◽  
Zhongfang Tan ◽  
Zongwei Li ◽  
Ya Li ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Raw Materials ◽  
Maturity Stages ◽  
Dry Matter Digestibility ◽  
Lactobacillus Paraplantarum ◽  
Significant Difference ◽  
Acid Detergent Fibre ◽  
Fermentation Quality

This study aimed to investigate the microorganism profile, fermentation quality and rumen digestibility in vitro of maize-stalk silage at different maturity stages. Maize-stalk samples were harvested at the stages milk-ripe, dough, fully ripe, and fully ripe exposed to air for 3 or 10 days. Silage pH, ammonia-N and chemical composition were measured. Thirteen representative lactic acid bacteria (LAB) strains isolated from the raw materials were categorised into five profile clusters: Leuconostoc citreum (23.1%), Weissella paramesenteroides (15.4%), Lactococcus garvieae (23.1%), Enterococcus faecalis (7.7%), and Lactobacillus paraplantarum (30.8%). The total LAB numbers in silages with raw materials reaped at the stages milk-ripe, dough, fully ripe, and fully ripe exposed to air for 3 or 10 days, respectively, were approximately 8, 6, 10, 3.5 and 10 log CFU g–1. The dominant LAB types of maize-stalk silage at different stages were all different. The epiphytic pathogens Escherichia coli, aerobic bacteria, filamentous fungi and Saccharomycetes were found in silages of all stages. There were significant differences (P < 0.001) in crude protein, ether extract, dry matter, acid detergent fibre and organic matter of silage at different stages; however, no significant difference (P > 0.05) was observed in dry matter digestibility after 24 h of fermentation in vitro, with NH3-N varying from 0.7 ± 0.1 to 1.7 ± 0.3 mg L–1.

scholarly journals Bromatological composition and dry matter digestibility of millet cultivars subjected to nitrogen doses

2014 ◽  
Vol 66 (3) ◽  
pp. 887-893 ◽  
Author(s):  
W.H.D. Buso ◽  
A.F.S. França ◽  
E.S. Miyagi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Block Design ◽  
Control Treatment ◽  
Average Height ◽  
Dry Matter Digestibility ◽  
Acid Detergent Fibre ◽  
N Dose ◽  
Sowing Season

The bromatological composition and in vitro dry matter digestibility of millet cultivars were assessed for different nitrogen doses and two sowing seasons in the Ceres municipality of Goiás state, Brazil. The treatments consisted of three millet cultivars (ADR-7010, ADR-500 and BRS-1501), four nitrogen (N) doses (0, 50, 100 and 200kg ha-1 of N) and two sowing seasons. Three replicates and a randomised block design with a 3 x 4 x 2 factorial scheme were used. Two cuttings were performed in each season when plants reached an average height of 0.70 cm. No significant interactions were observed between or among cultivars for N doses and dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents. The DM, CP, NDF and ADF contents were significantly different between N doses. The DM and CP contents increased as the N dose increased to 100kg ha-1. The maximum DM and CP contents were 11.14 and 22.53%, respectively. The NDF and ADF contents were higher in the control treatment (60.11 and 30.01%, respectively). In addition, the lowest ADF and NDF concentrations occurred at an N dose of 50kg ha-1(56.33 and 30.23%, respectively). The DM contents were higher for the February sowing, with an average of 10.59%. The highest CP and ADF contents were found for the December sowing (22.46 and 31.58%, respectively). No significant differences were found for millet cultivars, N doses or sowing seasons. A significant interaction was found between sowing season and millet cultivar. The BRS-1501 cultivar had a higher in vitro dry matter digestibility in the December/2010 sowing (73.88%).

scholarly journals The Effect of Nutmeg Leaves Tannin (Myristica fragrans Houtt) as Protein Protecting Agents on In Vitro Nutrient Digestibility

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Monica Canadianti ◽  
Lies Mira Yusiati ◽  
Chusnul Hanim ◽  
Budi Prasetyo Widyobroto ◽  
Andriyani Astuti
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
In Vitro Technique ◽  
Significant Difference ◽  
Organic Matter Digestibility

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.

EFFECTS OF EARLY SUMMER AND FALL CUTTING MANAGEMENT ON DRY MATTER YIELDS, DIGESTIBILITY, CRUDE PROTEIN, AND SURVIVAL OF SARANAC AND NARRAGANSETT ALFALFAS

1972 ◽  
Vol 52 (6) ◽  
pp. 941-948 ◽  
Author(s):  
L. B. MacLEOD ◽  
H. T. KUNELIUS ◽  
F. W. CALDER
Keyword(s):  
Medicago Sativa ◽  
Crude Protein ◽  
Dry Matter ◽  
Early Summer ◽  
Good Survival ◽  
Considerable Decrease ◽  
Dry Matter Digestibility ◽  
Medicago Sativa L ◽  
Cutting Management

The effects of early summer and fall cutting managements on two alfalfa (Medicago sativa L.) cultivars were studied over a 3-year period. Removal of the first crop of Saranac and Narrangansett alfalfas at prebud stage, followed by two cuts at the early bloom stage before early September, and a late fall harvest on October 25, produced seasonal dry matter (DM) yields of 7.5–8.6 metric T/ha in the 1st year. Stand deterioration, however, was severe and by the 3rd year, practically all alfalfa had disappeared. Average percent crude protein (CP) in cut 1 was 17.7–21.9 and percent in vitro dry matter digestibility (IVD) 67.4–73.8. Removal of the first cut at bud stage with two subsequent harvests at the early bloom stage also allowed a total of three harvests before early September and resulted in seasonal DM yields of 8.6–8.8 in the 1st year. Although the survival of alfalfa was increased over the managements where cut 1 was at prebud stage, a considerable decrease of alfalfa was still observed in the 3rd year. Under this management, average CP in cut 1 was 15.8–16.3 and IVD 64.1–68.4. Harvesting the first crop at early to midbloom stage and the second crop at early bloom in early to mid-August, followed by third harvest in late October, resulted in good survival with seasonal DM yields of 6.1–10.6. Lower CP and IVD were recorded in cut 1 than in the more intensive managements. Under all managements, average CP in cuts 2 and 3 was 14.9–17.3 and 13.3–19.4, respectively. IVD was 54.5–60.7 in cut 2 and 56.0–64.4 in cut 3.

scholarly journals KECERNAAN IN VITRO SILASE SAMPAH SAYUR DAN DAUN GAMAL MENGGUNAKAN MIKROORGANISME RUMEN KAMBING

2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Dry Matter ◽  
Vegetable Waste ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.

scholarly journals Effect of seedbed methods and time of harvest on the yield and nutritive value of some forage crops grown on Vertisol at Debre Zeit, Ethiopia

1992 ◽  
Vol 1 (5) ◽  
pp. 491-497
Author(s):  
Abate Tedla ◽  
Helena Airaksinen ◽  
M. A. Mohamed-Saleem
Keyword(s):  
Avena Sativa ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Dry Matter Yield ◽  
Lablab Purpureus ◽  
Forage Crops ◽  
Dry Matter Digestibility ◽  
Time Of Harvest

The influence of the improved drainage broadbed and furrow (BBF), as opposed to the traditional flat seedbed over the growing season on the dry matter yield and nutritive value of Avena sativa, Vigna unguiculata, Lablab purpureus, Vida dasycarpa, Trifolium steudneri and Sesbania sesban were studied on Vertisol. Up to 7 t/ha dry matter yield was recorded for Avena sativa and Lablab purpureus when planted on Vertisols with improved drainage. Dry matter yield of forage crops also increased with advance in stage of maturity or subsequent harvests. On both improved and traditional flat seedbed methods, the chemical analysis of forage crops showed similar declines in crude protein content and in vitro dry matter digestibility (IVDMD) levels as the maturity of forage crops progressed.

scholarly journals Effect of Sulphate ion on the in Vitro Rumen Digestion of Polysaccharide Constituents of Bagasse

1972 ◽  
Vol 25 (6) ◽  
pp. 1377 ◽  
Author(s):  
RFH Dekker ◽  
GN Richards
Keyword(s):  
Feed Intake ◽  
Dry Matter ◽  
Poor Quality ◽  
Crude Fibre ◽  
Heteropogon Contortus ◽  
Dry Matter Digestibility ◽  
Inorganic Sulphate ◽  
Acid Detergent Fibre

The dry matter digestibility (DMD) of poor quality roughages such as spear grass (Heteropogon contortus) by sheep and cattle has been shown to increase on addition of sulphur to the diet. The sulphur is added most frequently as inorganic sulphate, either alone (Playne 1969) or supplemented with urea (Bray and Hemsley 1969; Kennedy and Siebert 1972; Siebert and Kennedy 1972) or gluten (Playne 1969). These supplements have also increased feed intake in sheep (Playne 1969; Kennedy and Siebert 1972; Siebert and Kennedy 1972) and cattle (Siebert and Kennedy 1972), and have improved the nitrogen and sulphur balance (Moir, Somers, and Bray 1967; Bray and Hemsley 1969; Playne 1969; Kennedy and Siebert 1972) within the animal. Sulphates have been shown to increase the in vitro rumen digestion of starch (Kennedy, Mitchell, and Little 1971), crude fibre (Bray and Hemsley 1969), and acid-detergent fibre and cellulose (Barton, Bull, and Hemken 1971).

Dry matter intake, nutrient selection and milk production of dairy cows grazing rainfed perennial pastures at different herbage allowances in spring

1999 ◽  
Vol 39 (8) ◽  
pp. 923 ◽  
Author(s):  
P. J. Moate ◽  
D. E. Dalley ◽  
J. R. Roche ◽  
C. Grainger
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Herbage Intake ◽  
Selection Differentials

Summary. The effect of herbage allowance (20, 30, 40, 50, 60 and 70 kg DM/cow. day) on the consumption of nutrients from herbage and milk production by cows in early lactation, was examined. The experiment was conducted on rainfed perennial ryegrass pastures in September and October 1997 in south-eastern Victoria, Australia. The herbage on offer comprised 64% perennial ryegrass, 21% other grasses, 1% white clover, 5% weeds and 9% dead material on a dry matter (DM) basis. The average pregrazing herbage height was 13 cm, at an estimated pregrazing herbage mass of 3.6 t DM/ha. The herbage on offer was of high quality containing 11.6 MJ metabolisable energy/kg DM, 202 g crude protein/kg DM and 525 g neutral detergent fibre/kg DM. Concentrations of calcium, magnesium, sodium, potassium, phosphorus, sulfur and chloride were 4.4, 2.2, 4.4, 31.2, 3.5, 2.7 and 11.4 g/kg DM, respectively. As daily herbage allowance per cow increased, dry matter intake increased curvilinearly (P<0.01) from 11.2 to 18.7 kg DM/cow. day. This was associated with a decrease in utilisation of herbage from 54 to 26% and an increase in milk production from 25.9 to 29.1 kg/cow. day. The cows on all treatments grazed for less than 8.7 h/day. The increase in intake was achieved by an increase in the rate of herbage intake from 1.5 to 2.2 kg DM/h for herbage allowances of 20 and 70 kg/cow.day, respectively. Irrespective of herbage allowance, cows selected a diet that was approximately 10% higher in in vitro dry matter digestibility and 30% higher in crude protein than that in the herbage on offer. The neutral detergent fibre content of the herbage selected was lower (P<0.05) than that on offer. The herbage consumed contained more (P<0.05) magnesium, potassium and sulfur, the same amount of calcium and phosphorus and less (P<0.05) sodium and chloride than the herbage on offer. For rainfed perennial pastures in spring, herbage allowance is an important factor in determining voluntary feed intake and production of dairy cows. To achieve 30 L from herbage, without supplementation, high herbage allowances are required. The increase in herbage intake, with increasing herbage allowance, resulted from an increase in rate of dry matter intake and not an increase in grazing time. No relationship was evident between herbage allowance and the selection differentials for in vitro dry matter digestibility, crude protein and neutral detergent fibre. Selection differentials for rainfed perennial pastures in spring are similar to those reported for irrigated perennial pastures in northern Victoria in spring and autumn. When determining nutrient requirements it is important to consider the interaction between herbage intake and nutrient concentration in the herbage.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

scholarly journals Chemical characteristics, aerobic stability, and microbiological counts in corn silage re-ensiled with bacterial inoculant

2018 ◽  
Vol 53 (9) ◽  
pp. 1045-1052
Author(s):  
Mateus Merlo Coelho ◽  
Lúcio Carlos Gonçalves ◽  
José Avelino Santos Rodrigues ◽  
Kelly Moura Keller ◽  
Gustavo Vinícius de Souza dos Anjos ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Aerobic Stability ◽  
Whole Plant

Abstract: The objective of this work was to evaluate the effects of re-ensiling and bacterial inoculation on the quality of corn silage. The experiment was carried out in a 2x2 factorial design with or without inoculant (association of Lactobacillus plantarum and Propionibacterium acidipropionici), and with re-ensiling after 36 hours of aerobic exposure or only ensiling of the whole plant of 'BRS 1055' corn. The fermentative quality, nutritional parameters, dry matter losses, aerobic stability, and microbiological counts of silages were evaluated. Re-ensiling caused an increase of pH and in acetic acid and propionic acid concentrations, as well as in the dry matter (DM), crude protein, neutral detergent fiber, and neutral detergent fiber crude protein contents. Conversely, there was a reduction in the nonfiber carbohydrates concentration and in in vitro dry matter digestibility for the re-ensiled material. All changes were explained by the higher-effluent production and DM loss of re-ensiled material that was subjected to two compactions. Microbiology was not altered by the treatments. The use of inoculant altered ash content, but it did not influence other parameters. In contrast, re-ensiling after 36 hours of aerobic exposure caused a reduction in the nutritive value of corn silage and accentuated the DM losses.

scholarly journals Methanogenic potential of forages consumed throughout the year by cattle in a Sahelian pastoral area

2016 ◽  
Vol 56 (3) ◽  
pp. 613 ◽  
Author(s):  
M. Doreau ◽  
H. Benhissi ◽  
Y. E. Thior ◽  
B. Bois ◽  
C. Leydet ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Near Infrared ◽  
Pastoral Systems ◽  
Ch4 Production ◽  
Pastoral Area ◽  
Acid Detergent Fibre ◽  
Dry Seasons ◽  
Plant Components

Methane (CH4) emission from ruminants in African pastoral systems may be affected by intake and type of plants, which vary highly between rainy and dry seasons. In each of two sites located in the semiarid Sahelian area of Senegal, three Gobra zebus were monitored throughout 1 year. A representative sample of their diet was obtained once every month. Diet was mainly composed of grasses, herbaceous legumes, tree and shrub foliage and pods, and dried forage residues. CH4 production and volatile fatty acid (VFA) concentration, which reflects VFA production, were determined in vitro. Crude protein, neutral detergent fibre (NDF) and acid detergent fibre were measured by near-infrared spectrophotometry. CH4 production varied between 24.6 and 35.2 mL/g forage dry matter (DM), being minimal in August (rainy season) and maximal in February (dry season). Seasonal difference disappeared when CH4 was expressed in mL/g NDF. The acetate : propionate ratio varied in the same way as CH4 (3.2 and 4.6 in August and February, respectively); VFA concentration was minimum in March and maximum in September (69.2 and 77.4 mmol/L, respectively). CH4 production was closely related to dietary NDF content (r = 0.82) and to acetate : propionate ratio (r = 0.96). For six successive periods (February to July), plant categories constituting the diet were incubated separately. Reconstituting the CH4 production and VFA concentration in the diet on the basis of the proportion of plant components gave values similar to those of the global diet (33.4 and 34.2 mL CH4/g DM and 75.9 and 70.9 mmol VFA/L, respectively). This result suggests the absence of interaction among plant components on rumen fermentation.

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