NB-LRR gene family required for Rsc4-mediated resistance to Soybean mosaic virus

2016 ◽  
Vol 67 (5) ◽  
pp. 541 ◽  
Author(s):  
Na Li ◽  
Jin Long Yin ◽  
Cui Li ◽  
Da Gang Wang ◽  
Yong Qing Yang ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Candidate Genes ◽  
Soybean Mosaic Virus ◽  
Polymerase Chain Reaction Analysis ◽  
Genomic Region ◽  
Chromosome 14 ◽  
Chain Reaction ◽  
Bean Pod Mottle Virus ◽  
Genes Encoding ◽  
Polymerase Chain

Soybean mosaic virus (SMV) causes one of the most destructive viral diseases in soybean (Glycine max). The soybean cultivar Dabaima carries the Rsc4 gene for SMV resistance. The genomic region containing Rsc4 was previously localised within a 100-kb region on chromosome 14. The corresponding region contains three complete nucleotide-binding site (NB) and leucine-rich repeat (LRR) type genes and one incomplete gene that is likely non-functional. Quantitative real-time polymerase chain reaction analysis revealed that three candidate genes encoding NB-LRR proteins were differentially expressed in resistant and susceptible lines when the plants were inoculated with SMV strain SC4. To test the involvement of the three candidate genes in Rsc4 mediated resistance, the three genes were silenced using a Bean pod mottle virus (BPMV)-based vector construct. Silencing of three candidate genes attenuated the Rsc4-mediated resistance and induced SMV symptoms in Dabaima plants. Moreover, Rsc4 candidate genes were 78% downregulated when compared with the empty BPMV vector-treated plants. From these results, we concluded that at least one of the three candidate genes encoding NB-LRR proteins is required for Rsc4 resistance to SMV.

scholarly journals Soybean Cytochrome b5 Is a Restriction Factor for Soybean Mosaic Virus

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 546 ◽  
Author(s):  
Hexiang Luan ◽  
Haopeng Niu ◽  
Jinyan Luo ◽  
Haijian Zhi
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Cytochrome B5 ◽  
Polymerase Chain Reaction Analysis ◽  
Bimolecular Fluorescence Complementation ◽  
Cell Periphery ◽  
Bean Pod Mottle Virus ◽  
Protein Targets ◽  
Bimolecular Fluorescence Complementation Assay ◽  
Soybean Plants

Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybeans (Glycine max). In this study, an interaction between the SMV P3 protein and cytochrome b5 was detected by yeast two-hybrid assay, and bimolecular fluorescence complementation assay showed that the interaction took place at the cell periphery. Further, the interaction was confirmed by co-immunoprecipitation analysis. Quantitative real-time polymerase chain reaction analysis revealed that GmCYB5 gene was differentially expressed in resistant and susceptible soybean plants after inoculation with SMV-SC15 strain. To test the involvement of this gene in SMV resistance, the GmCYB5 was silenced using a bean pod mottle virus (BPMV)-based vector construct. Results showed that GmCYB5-1 was 83% and 99% downregulated in susceptible (NN1138-2) and resistant (RN-9) cultivars, respectively, compared to the empty vector-treated plants. Silencing of GmCYB5 gene promotes SMV replication in soybean plants. Our results suggest that during SMV infection, the host CYB5 protein targets P3 protein to inhibit its proliferation. Taken together, these results suggest that CYB5 is an important factor in SMV infection and replication in soybeans, which could help soybean breeders develop SMV resistant soybean cultivars.

scholarly journals The Yeast Signal Sequence Trap Identifies Secreted Proteins of the Hemibiotrophic Corn Pathogen Colletotrichum graminicola

2008 ◽  
Vol 21 (10) ◽  
pp. 1325-1336 ◽  
Author(s):  
Jorrit-Jan Krijger ◽  
Ralf Horbach ◽  
Michael Behr ◽  
Patrick Schweizer ◽  
Holger B. Deising ◽  
...  
Keyword(s):  
Cell Walls ◽  
Leaf Extract ◽  
Signal Sequence ◽  
Stem Rot ◽  
Colletotrichum Graminicola ◽  
In Planta ◽  
Chain Reaction ◽  
Genes Encoding ◽  
Polymerase Chain

The hemibiotroph Colletotrichum graminicola is the causal agent of stem rot and leaf anthracnose on Zea mays. Following penetration of epidermal cells, the fungus enters a short biotrophic phase, followed by a destructive necrotrophic phase of pathogenesis. During both phases, secreted fungal proteins are supposed to determine progress and success of the infection. To identify genes encoding such proteins, we constructed a yeast signal sequence trap (YSST) cDNA-library from RNA extracted from mycelium grown in vitro on corn cell walls and leaf extract. Of the 103 identified unigenes, 50 showed significant similarities to genes with a reported function, 25 sequences were similar to genes without a known function, and 28 sequences showed no similarity to entries in the databases. Macroarray hybridization and quantitative reverse-transcriptase polymerase chain reaction confirmed that most genes identified by the YSST screen are expressed in planta. Other than some genes that were constantly expressed, a larger set showed peaks of transcript abundances at specific phases of pathogenesis. Another set exhibited biphasic expression with peaks at the biotrophic and necrotrophic phase. Transcript analyses of in vitro-grown cultures revealed that several of the genes identified by the YSST screen were induced by the addition of corn leaf components, indicating that host-derived factors may have mimicked the host milieu.

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