Large-scale density-based screening for pea weevil resistance in advanced backcross lines derived from cultivated field pea (Pisum sativum) and Pisum fulvum

2012 ◽  
Vol 63 (7) ◽  
pp. 612 ◽  
Author(s):  
N. Aryamanesh ◽  
O. Byrne ◽  
D. C. Hardie ◽  
T. Khan ◽  
K. H. M. Siddique ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Large Scale ◽  
Interspecific Cross ◽  
Field Pea ◽  
Separation Method ◽  
Density Separation ◽  
Advanced Backcross ◽  
Pisum Fulvum ◽  
Resistant Lines ◽  
Backcross Populations

The pea weevil, Bruchus pisorum, is one of the most intractable pest problems of cultivated field pea (Pisum sativum) in the world. Pesticide application, either as a contact insecticide spray to the field pea crop or fumigation of the harvested seed, is the only available method for its control. The aim of the study was to develop a quick and reliable method to screen for pea weevil resistance and increase efficiency in breeding for this important trait. Backcrossed progenies derived from an interspecific cross between cultivated field pea and its wild relative (Pisum fulvum, source of resistance for pea weevil) were subjected to natural infestation in field plots. Mature seeds were hand-harvested, stored to allow development of adult beetles, and then separated into infested and non-infested using a density separation method in 30% caesium chloride (CsCl). Susceptibility and resistance of the progenies were calculated based on this method and further confirmed by a glasshouse bioassay. Resistance in backcross populations improved considerably through selection of resistant lines using the density separation method. We found that the method using CsCl separation is a useful tool in breeding for pea weevil resistance. We were able to introgress pea weevil resistance from P. fulvum into cultivated field pea through backcrossing to produce several advanced pea weevil resistant lines following this procedure.

Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum×P. fulvum interspecific cross

2008 ◽  
Vol 59 (9) ◽  
pp. 854 ◽  
Author(s):  
O. M. Byrne ◽  
D. C. Hardie ◽  
T. N. Khan ◽  
J. Speijers ◽  
G. Yan
Keyword(s):  
Pisum Sativum ◽  
Interspecific Cross ◽  
Transgressive Segregation ◽  
Field Pea ◽  
Current Field ◽  
F2 Population ◽  
Pisum Fulvum ◽  
Inheritance Model ◽  
Large Populations ◽  
Seed Resistance

Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F2 population, with evidence of transgressive segregation. Heritability of pod resistance between F2 and F3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F2 population by testing F3 seed tissues of individual F2 plants and pooling data from seed reaction for each F2 plant (inferred F2 genotype). Segregation for seed resistance in the F2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F2 to F5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr1, pwr2, and pwr3, and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (>300 F2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.

scholarly journals Characterization of Physiological Resistance to White Mold and Search for Molecular Markers Linked to Resistance via Advanced Backcross QTL Analysis in an Interspecific Cross between Phaseolus coccineus and P. vulgaris

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 973B-973 ◽  
Author(s):  
J. Erron Haggard ◽  
James R. Myers
Keyword(s):  
Common Bean ◽  
Interspecific Cross ◽  
White Mold ◽  
Resistance Qtl ◽  
Additional Information ◽  
Advanced Backcross ◽  
Backcross Populations ◽  
Scarlet Runner Bean ◽  
Mold Resistance ◽  
High Level

White mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, causes major losses in dry and snap bean (Phaseolus vulgaris) production. With little genetic variation for white mold resistance in common bean, other potential sources for resistance must be investigated. Accessions of scarlet runner bean (P. coccineus) have been shown to have partial resistance exceeding any to be found in common bean. Resistance is quantitative with at least six QTL found in a P. coccineus intraspecific resistant × susceptible cross. Our goal is to transfer high levels of resistance from P. coccineus into commercially acceptable common bean lines. We developed interspecific advanced backcross populations for mapping and transfer of resistance QTL. 111 BC2F5 lines from a cross between OR91G and PI255956 have been tested in straw tests and oxalate tests, as well as in a field trial. The data show that the OR91G × PI255956 population carries a high level of resistance, but because of the quantitative nature of resistance, it may be necessary to intercross individuals to achieve higher levels. SSR, RAPD, and AFLP markers are being tested in the population to construct a linkage map for placement of QTL. QTL identified from each type of test (straw, oxalate, and field) may provide additional information about the genetic architecture of white mold resistance. Three other populations are from advanced backcrosses of the recurrent parents G122, OR91G, and MO162, with PI433251B as the donor parent in each. Analyses and advance of these populations will follow, the results of which should confirm QTL identified in the OR91G × PI255956 population, as well as possible additional resistance QTL from PI433251B.

scholarly journals Higher glandular trichome density in tomato leaflets and repellence to spider mites

2007 ◽  
Vol 42 (9) ◽  
pp. 1227-1235 ◽  
Author(s):  
Wilson Roberto Maluf ◽  
Irene Fumi Inoue ◽  
Raphael de Paula Duarte Ferreira ◽  
Luiz Antonio Augusto Gomes ◽  
Evaristo Mauro de Castro ◽  
...  
Keyword(s):  
Leaf Surface ◽  
Interspecific Cross ◽  
Glandular Trichomes ◽  
Glandular Trichome ◽  
Spider Mites ◽  
Tomato Leaf ◽  
Advanced Backcross ◽  
Leaf Surfaces ◽  
Backcross Populations ◽  
Selection For

The objective of this work was to evaluate the feasibility of selection for higher glandular trichome densities, as an indirect criterion of selection for increasing repellence to spider mites Tetranychus urticae, in tomato populations derived from an interspecific cross between Lycopersicon esculentum x L. hirsutum var. glabratum PI 134417. Trichome densities were evaluated in 19 genotypes, including 12 from advanced backcross populations, derived from the original cross L. esculentum x L. hirsutum var. glabratum PI 134417. Counts were made both on the adaxial and abaxial leaf surfaces, and trichomes were classified into glandular types IV and VI, other glandular types (types I+VII), and nonglandular types. Mite repellence was measured by distances walked by mites onto the tomato leaf surface after 20, 40 and 60 min. Spider mite repellence biotests indicated that higher densities of glandular trichomes (especially type VI) decreased the distances walked by the mites onto the tomato leaf surface. Selection of plants with higher densities of glandular trichomes can be an efficient criterion to obtain tomato genotypes with higher resistance (repellence) to spider mites.

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Field Pea ◽  
Pisum Sativum L

DS Admiral field pea

2002 ◽  
Vol 82 (4) ◽  
pp. 751-752 ◽  
Author(s):  
L. Andersen ◽  
T. Warkentin ◽  
O. Philipp ◽  
A. Xue ◽  
A. Sloan
Keyword(s):  
Powdery Mildew ◽  
Pisum Sativum ◽  
Powdery Mildew Resistance ◽  
Field Pea ◽  
Western Canada ◽  
Mildew Resistance ◽  
Pisum Sativum L ◽  
Cultivar Description ◽  
Good Yielding ◽  
Growing Region

DS Admiral, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2000 by Agriprogress Inc., Morden, Manitoba. DS Admiral has a semileafless leaf type, powdery mildew resistance, medium sized, round seeds, and good yielding ability. DS Admiral is adapted to the field-pea-growing region of western Canada. Key words: Field pea, Pisum sativum L., cultivar description, powdery mildew resistance

BELLEVUE FIELD PEA

1987 ◽  
Vol 67 (3) ◽  
pp. 805-806
Author(s):  
A. E. SLINKARD ◽  
R. A. A. MORRALL
Keyword(s):  
Pisum Sativum ◽  
Seed Weight ◽  
Field Pea ◽  
Pisum Sativum L ◽  
Cultivar Description

Bellevue field pea (Pisum sativum L.) was licensed in 1986. It is about 15% higher yielding than the check cultivars Century and Trapper. Bellevue has smooth seeds with yellow cotyledons. It has the le gene for shortened internodes and is about 6 d later maturing than Century and Trapper. Seed weight is about 179 g/1000 seeds, intermediate between Century and Trapper.Key words: Cultivar description, pea (field), Pisum sativum L.

Proximate and mineral composition of field peas

1997 ◽  
Vol 77 (1) ◽  
pp. 101-103 ◽  
Author(s):  
T. D. Warkentin ◽  
A. G. Sloan ◽  
S. T. Ali-Khan
Keyword(s):  
Pisum Sativum ◽  
Key Words ◽  
Mineral Composition ◽  
Seed Yield ◽  
Total Protein ◽  
Field Pea ◽  
Pisum Sativum L ◽  
Field Peas ◽  
Pea Seeds ◽  
Proximate And Mineral Composition

Field pea seeds from 10 cultivars grown at two locations in Manitoba in 1986 and 1987 were analyzed for proximate and mineral profiles. Cultivars differed significantly in their level of total protein, crude fat, ADF, and all minerals tested. However, differences were not extremely large and were comparable to European reports. Location-year also had a significant effect on the levels of total protein, ADF, and all minerals tested. In most cases, the warmest location-year produced relatively higher levels of minerals, ash, and total protein, and lower seed yield than the coolest location-year. Key words: Field pea, Pisum sativum L., mineral

Seasonal Incidence of Pulse Aphid (Aphis craccivora Koch.) and its Natural Enemies on Field Pea (Pisum sativum L.) in Relation to Some Abiotic Factors in Alluvial Zone of West Bengal

2021 ◽  
Author(s):  
S. Pal ◽  
S. Samanta ◽  
A. Banerjee
Keyword(s):  
Relative Humidity ◽  
Pisum Sativum ◽  
Natural Enemies ◽  
West Bengal ◽  
Insect Pests ◽  
Field Pea ◽  
Pest Population ◽  
Pisum Sativum L ◽  
Aphis Craccivora Koch ◽  
Weather Parameters

Background: Field pea, Pisum sativum L. is an important winter-season pulse crop. It is subjected to damage by both field and storage insect pests and approximately 10-15 per cent reduction in yield was reported due to the infestation of different insect pests. Among these, pulse aphid (Aphis craccivora Koch.) affects plant physiology directly by removal of nutrients or indirectly by dispersal of various viral diseases. The present investigation has been aimed to study the seasonal fluctuations of aphids and their natural enemies as well as their correlation. Another objective was to know the effect of various weather parameters on pulse aphids and their natural enemies which ultimately would be helpful to develop a forewarning model.Methods: The field experiment was conducted at the A-B Block Farm of Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal using two varieties of field pea (KPMR 935 and IFPD 122) during rabi seasons of two consecutive years of 2017-18 and 2018-19 following a Randomized Block Design with three replications. After recording the total population of both pests (nymphs and adults) and their natural enemies across the season, the mean population was worked out and used for correlation and regression studies along with the weather parameters. Result: Maximum aphid population was noticed during the peak pod formation stage of the crop irrespective of the varieties. The pest population was very strongly correlated with the incidence of coccinellid and ant population in both test varieties. Among the weather parameters, both maximum and minimum temperature and sunshine hour showed a positive correlation with the pest population and their natural enemies but relative humidity and rainfall showed a negative correlation. Regression studies indicated that temperature and relative humidity were the most influencing factors over the incidence of aphid in both the seasons.

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