Novel Uperin Peptides From the Dorsal Glands of the Australian Floodplain Toadlet Uperoleia inundata

1996 ◽  
Vol 49 (4) ◽  
pp. 475 ◽  
Author(s):  
AM Bradford ◽  
MJ Raftery ◽  
JH Bowie ◽  
MJ Tyler ◽  
JC Wallace ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequences ◽  
Host Defence ◽  
Edman Sequencing ◽  
Antibiotic Peptides

The dorsal glandular extract of the floodplain toadlet Uperoleia inundata contains more than 50 peptides: we report the amino acid sequences and bioactivity data of 13 of these. The peptides have been sequenced by using a combination of mass spectrometry and automated Edman sequencing. Ten of the peptides have been synthesized in order to confirm their structures, and to enable bioassays to be carried out. Ten peptides are host- defence agents, including ( i ) a powerful new neuropeptide of the tachykinin family which we name uperin 1.1 [ pGlu Ala Asp Pro Asn Ala Phe Tyr Gly Leu Met (NH2)], and (ii) nine antibiotic peptides including five uperins 2 [e.g. uperin 2.1, Gly Ile Val Asp Phe Ala Lys Lys Val Val Gly Gly Ile Arg Asn Ala Leu Gly Ile (OH)], three uperins 3 [e.g. uperin 3.1, Gly Val Leu Asp Ala Phe Arg Lys Ile Ala Thr Val Val Lys Asn Val Val (NH2)] and uperin 4.1 [ Gly Val Gly Ser Phe Ile His Lys Val Val Ser Ala Ile Lys Asn Val Ala (NH2)]. The function of the three other characterized peptides in the amphibian integument is not known, viz. ( i ) uperin 5.1 [ Phe Gln Phe Val Asn Pro Ser Asp Ile Val Phe Gly Ser (OH)] and (ii) the two uperins 6 [e.g. uperin 6.1, Gly Leu Ala Gly Ala Ile Ser Ser Ala Leu Asp Lys Leu Lys Gln Ser Gln Leu Ile Lys Asn Tyr Ala Lys Lys Leu Gly Tyr Pro Arg (OH)].

New Antibiotic Uperin Peptides From the Dorsal Glands of the Australian Toadlet Uperoleia mjobergii

1996 ◽  
Vol 49 (12) ◽  
pp. 1325 ◽  
Author(s):  
AM Bradford ◽  
JH Bowie ◽  
MJ Tyler ◽  
JC Wallace
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Related Species ◽  
Antibiotic Activity ◽  
Amino Acid Sequences ◽  
The Other ◽  
Cationic Peptides ◽  
Amino Acid Residues ◽  
Skin Glands ◽  
Edman Sequencing

The dorsal glandular extract of the toadlet Uperoleia mjobergii contains more than 20 peptides. We report the amino acid sequences of the seven major peptides: these were determined by a combination of mass spectrometry and automated Edman sequencing. Three of these peptides have 19 amino acid residues and belong to the uperin 2 group of peptides [e.g. uperin 2.6, Gly Ile Leu Asp Ile Ala Lys Lys Leu Val Gly Gly Ile Arg Asn Val Leu Gly Ile (OH)], while the other four have 17 residues and are classified as uperins 3 [e.g. Uperin 3.4, Gly Val Gly Asp Leu Ile Arg Lys Ala Val Ala Ala Ile Lys Asn Ile Val (NH2)]. Several of these cationic peptides have been synthesized in order for bioassays to be carried out: they show significant antibiotic activity against a range of Gram-positive microorganisms. A major skin peptide from the related species Uperoleia inundata is a powerful neuropeptide named uperin 1.1 ([Ala2] uperolein ): no corresponding neuropeptide is detected in the skin glands of Uperoleia mjobergii.

Resolution of a protein sequence ambiguity by X-ray crystallographic and mass spectrometric methods

1992 ◽  
Vol 25 (2) ◽  
pp. 205-210 ◽  
Author(s):  
L. J. Keefe ◽  
E. E. Lattman ◽  
C. Wolkow ◽  
A. Woods ◽  
M. Chevrier ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Electron Density ◽  
Mass Spectrometric ◽  
Amino Acid Sequences ◽  
Data Matrix ◽  
Protein Crystal ◽  
Insertion Mutant ◽  
Laser Desorption Mass Spectrometry ◽  
X Ray

Ambiguities in amino acid sequences are a potential problem in X-ray crystallographic studies of proteins. Amino acid side chains often cannot be reliably identified from the electron density. Many protein crystal structures that are now being solved are simple variants of a known wild-type structure. Thus, cloning artifacts or other untoward events can readily lead to cases in which the proposed sequence is not correct. An example is presented showing that mass spectrometry provides an excellent tool for analyzing suspected errors. The X-ray crystal structure of an insertion mutant of Staphylococcal nuclease has been solved to 1.67 Å resolution and refined to a crystallographic R value of 0.170 [Keefe & Lattman (1992). In preparation]. A single residue has been inserted in the C-terminal α helix. The inserted amino acid was believed to be an alanine residue, but the final electron density maps strongly indicated that a glycine had been inserted instead. To confirm the observations from the X-ray data, matrix-assisted laser desorption mass spectrometry was employed to verify the glycine insertion. This mass spectrometric technique has sufficient mass accuracy to detect the methyl group that distinguishes glycine from alanine and can be extended to the more common situation in which crystallographic measurements suggest a problem with the sequence, but cannot pinpoint its location or nature.

scholarly journals Study by mass spectrometry of amino acid sequences in peptides containing histidine

1970 ◽  
Vol 117 (2) ◽  
pp. 31P-32P ◽  
Author(s):  
J F G Vliegenthart ◽  
L Dorland
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequences

Mass spectrometric studies of peptides. V. Determination of amino acid sequences in peptide mixtures by mass spectrometry

1973 ◽  
Vol 95 (10) ◽  
pp. 3369-3375 ◽  
Author(s):  
Hans Kaspar. Wipf ◽  
Philip. Irving ◽  
Malcolm. McCamish ◽  
Rengachari. Venkataraghavan ◽  
F. W. McLafferty
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Mass Spectrometric ◽  
Amino Acid Sequences ◽  
Peptide Mixtures

scholarly journals Determination of amino acid sequences in peptides by mass spectrometry. Desulfurization of sulfur-containing peptides

FEBS Letters ◽  
1970 ◽  
Vol 8 (4) ◽  
pp. 207-209 ◽  
Author(s):  
R. Toubiana ◽  
J.E.G. Barnett ◽  
E. Sach ◽  
B.C. Das ◽  
E. Lederer
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequences ◽  
Sulfur Containing

Determination of amino acid sequences in oligopeptides by mass spectrometry. II. The structure of peptidolipin NA

1965 ◽  
Vol 6 (18) ◽  
pp. 1331-1336 ◽  
Author(s):  
M. Barber ◽  
W.A. Wolstenholme ◽  
M. Guinand ◽  
G. Michel ◽  
B.C. Das ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequences
Sign in / Sign up

Export Citation Format

Share Document