The structure determination of a new cembranolide diterpene from the soft coral Lobophytum cristigalli (Coelenterata, Octocorallia, Alcyonacea)

1984 ◽  
Vol 37 (3) ◽  
pp. 545 ◽  
Author(s):  
BF Bowden ◽  
JC Coll ◽  
MSL de Costa ◽  
MF Mackay ◽  
M Mahendran ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Structure Determination ◽  
Soft Coral ◽  
X Ray ◽  
X Ray Crystallography

A specimen of Lobophytum cristigalli collected in Sri Lanka afforded two cembranolide diterpenes (7E,11E,1R,2S,3R,4R,14S)-14-acetoxy-3,4-epoxycembra-7,11,15-trien-17,2-olide (6)* and the corresponding alcohol (7). Their structures were deduced spectroscopically and detailed stereochemistry of (6) was determined by X-ray crystallography.

Studies of Australian Soft Corals .XXXVII. The Structure Determination of 2 Cembranolide Diterpenes From Soft Corals of the Genus Efflatounaria (Coelenterata, Octocorallia, Alcyonacea)

1986 ◽  
Vol 39 (1) ◽  
pp. 123 ◽  
Author(s):  
BF Bowden ◽  
JC Coll ◽  
LM Engelhardt ◽  
GV Meehan ◽  
GG Pegg ◽  
...  
Keyword(s):  
Structure Determination ◽  
Soft Coral ◽  
Soft Corals ◽  
X Ray ◽  
X Ray Crystallography ◽  
Coral Genus ◽  
Relative Stereochemistry ◽  
Chemical Correlation ◽  
Absolute Stereochemistry

Two isomeric 7,8-epoxycembra-3,11,15-trien-16,2-olides have been isolated from different species of the soft coral genus Efflatounaria. The structure of each was deduced spectroscopically , and the relative stereochemistry of one was determined by X-ray crystallography. The absolute stereochemistry of each cembranolide was obtained by chemical correlation with known compounds.

Studies of Australian Soft Corals. XLI. Structure Determination of a New Sesquiterpene From Xenia novae-britanniae and an Investigation of a Xenia Species

1987 ◽  
Vol 40 (8) ◽  
pp. 1483 ◽  
Author(s):  
BF Bowden ◽  
JC Coll ◽  
LM Engelhardt ◽  
A Heaton ◽  
AH White
Keyword(s):  
Single Crystal ◽  
Structure Determination ◽  
Soft Coral ◽  
Soft Corals ◽  
X Ray ◽  
X Ray Crystallography ◽  
Relative Stereochemistry

.A novel sesquiterpene (1 Z,2R*,4R*,7S*,11S*)-3,3,7,11-tetramethyltricyclo[6.3.0.02,4]undec-1(8)-en-4-ol (3) was isolated from the soft coral Xenia novae-britanniae. Its structure was tentatively deduced by n.m.r. spectroscopy, and the structure with relative stereochemistry was confirmed by single-crystal X-ray crystallography. Investigation of a Xenia species afforded the known metabolite waixenicin -A previously isolated from Anthelia edmondsoni .

scholarly journals Structure determination of GPCRs: cryo-EM compared with X-ray crystallography

2021 ◽  
Author(s):  
Javier García-Nafría ◽  
Christopher G. Tate
Keyword(s):  
Structural Biology ◽  
Structure Determination ◽  
Drug Targets ◽  
Cellular Systems ◽  
Single Family ◽  
Structure Based Drug Design ◽  
X Ray ◽  
Surface Receptors ◽  
X Ray Crystallography

G protein-coupled receptors (GPCRs) are the largest single family of cell surface receptors encoded by the human genome and they play pivotal roles in co-ordinating cellular systems throughout the human body, making them ideal drug targets. Structural biology has played a key role in defining how receptors are activated and signal through G proteins and β-arrestins. The application of structure-based drug design (SBDD) is now yielding novel compounds targeting GPCRs. There is thus significant interest from both academia and the pharmaceutical industry in the structural biology of GPCRs as currently only about one quarter of human non-odorant receptors have had their structure determined. Initially, all the structures were determined by X-ray crystallography, but recent advances in electron cryo-microscopy (cryo-EM) now make GPCRs tractable targets for single-particle cryo-EM with comparable resolution to X-ray crystallography. So far this year, 78% of the 99 GPCR structures deposited in the PDB (Jan–Jul 2021) were determined by cryo-EM. Cryo-EM has also opened up new possibilities in GPCR structural biology, such as determining structures of GPCRs embedded in a lipid nanodisc and multiple GPCR conformations from a single preparation. However, X-ray crystallography still has a number of advantages, particularly in the speed of determining many structures of the same receptor bound to different ligands, an essential prerequisite for effective SBDD. We will discuss the relative merits of cryo-EM and X-ray crystallography for the structure determination of GPCRs and the future potential of both techniques.

scholarly journals Structure Determination of Membrane Proteins Using X-Ray Crystallography

2021 ◽  
pp. 101-136
Author(s):  
Evan Billings ◽  
Karl Lundquist ◽  
Claire Overly ◽  
Karthik Srinivasan ◽  
Nicholas Noinaj
Keyword(s):  
Membrane Proteins ◽  
Structure Determination ◽  
X Ray ◽  
X Ray Crystallography

scholarly journals Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography

2016 ◽  
Vol 2 (2-4) ◽  
pp. 55-68 ◽  
Author(s):  
Zheng Liu ◽  
Tom S. Y. Guu ◽  
Jianhao Cao ◽  
Yinyin Li ◽  
Lingpeng Cheng ◽  
...  
Keyword(s):  
Structure Determination ◽  
Human Virus ◽  
X Ray ◽  
X Ray Crystallography

Studies of Australian Soft Corals .XXXVI. The Isolation and Structure Determination of 11 Calamenene-Based Sesquiterpenes From Lemnalia-Cervicornis (Coelenterata, Octocorallia, Alcyonacea)

1986 ◽  
Vol 39 (1) ◽  
pp. 103 ◽  
Author(s):  
BF Bowden ◽  
JC Coll ◽  
LM Engelhardt ◽  
DM Tapiolas ◽  
AH White
Keyword(s):  
Structure Determination ◽  
Soft Coral ◽  
Soft Corals ◽  
X Ray ◽  
Chemical Correlation ◽  
Derivatives Of ◽  
Absolute Stereochemistry

The soft coral Lemnalia cervicornis May 1898 has afforded 13 novel sesquiterpenoid metabolites. The majority were derivatives of calamenen-7-ol and the structures were deduced by 1H and 13C n.m.r . spectroscopy. Absolute stereochemistry of compounds (3)-(13) was determined by chemical correlation with 7-hydroxycalamenen-3-one (3) for which an X-ray structure determination of its 7-p-bromobenzoyl derivative is reported. Two minor metabolites (15) and (16) were not aromatic, but were structurally related to the algal metabolite zonarene (21) for which absolute stereochemistry was available.

Sign in / Sign up

Export Citation Format

Share Document