Studies on the application of the quinhydrone electrode in formamide. I. The ionization constant of acetic acid from E.M.F. measurements at 25°

1973 ◽  
Vol 26 (1) ◽  
pp. 111 ◽  
Author(s):  
B Nayak ◽  
UN Dash
Keyword(s):  
Acetic Acid ◽  
Ionization Constant ◽  
Silver Chloride ◽  
Silver Chloride Electrode ◽  
Silver Silver ◽  
Silver Silver Chloride ◽  
Good Agreement

The quinhydrone electrode in conjunction with the silver-silver chloride electrode has been used in the cell ���������� Ag-AgCl | NaCl(m3), CH3CO2Na(m2), CH3CO2H(m1), QH2-Q | Pt to redetermine the ionization constant of acetic acid in formamide. The ionization constant is found to be 1.096 x 10-7 and is in good agreement with the value 1.230 x 10-7 obtained from the study of the cell ���������� Pt, H2|CH3CO2H(m1), CH3CO2Na(m2), NaCl(m3)|AgCl-Ag The results confirm the reversibility and reproducibility of the quinhydrone electrode in formamide.

Electrochemical investigation of hapten-antibody interactions by differential pulse polarography.

1982 ◽  
Vol 28 (9) ◽  
pp. 1968-1972 ◽  
Author(s):  
K R Wehmeyer ◽  
H B Halsall ◽  
W R Heineman
Keyword(s):  
Specific Antibody ◽  
Silver Chloride ◽  
Differential Pulse ◽  
Differential Pulse Polarography ◽  
Potential Range ◽  
Peak Current ◽  
Silver Chloride Electrode ◽  
Pulse Polarography ◽  
Silver Silver ◽  
Silver Silver Chloride

Abstract The binding of electroactively labeled estriol with estrogen-specific antibody and its subsequent displacement by unlabeled estriol have been monitored by differential pulse polarography. Estriol was found to be electro-inactive in the potential range -200 mV to -1000 mV vs a silver/silver chloride electrode. Estriol labeled in the 2 and 4 position with nitro groups was electroactive, giving two reduction waves at -422 mV and -481 mV vs a silver/silver chloride electrode. The peak current was linear with concentration over the range 60 micrograms/L to 3.7 mg/L. The addition of aliquots of estrogen-specific antibody reduced the peak current proportionately, indicating the binding of ligand to specific antibody. Subsequent addition of unlabeled estriol produced incremental increases in peak reduction current, indicating competitive and reversible binding of the two ligands for the antibody. Separation of bound from free labeled hapten was not necessary because reduction of the antibody-bound labeled estriol is attenuated at the electrode.

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