Venomic Interrogation Reveals the Complexity of Conus striolatus Venom

2020 ◽  
Vol 73 (4) ◽  
pp. 357
Author(s):  
S. W. A. Himaya ◽  
Subash K. Rai ◽  
Giulia Pamfili ◽  
Ai-Hua Jin ◽  
Paul F. Alewood ◽  
...  
Keyword(s):  
Potassium Channels ◽  
High Throughput ◽  
Proteomic Analysis ◽  
Cone Snail ◽  
Post Translational Modifications ◽  
Peptide Processing ◽  
Sodium Calcium ◽  
Venom Composition ◽  
Evolutionary Trajectories ◽  
Similar Gene

Given the complexity of cone snail venoms, high throughput venomics approaches are required to fully investigate venom composition, envenomation strategies, and evolutionary trajectories. This study describes 158 conotoxins in the venom transcriptome of the little studied C. striolatus from the fish hunting clade Pionoconus. Despite similar gene superfamily distributions along the venom duct, only 18 common transcripts were identified between distal, central, and proximal venom duct transcriptomes. Proteomic analysis of the injected predatory venom collected from the same individual revealed an ~18-fold enhanced complexity at the proteomic level, consistent with complex post-translational modifications and variable venom peptide processing occurring in the venom duct. Overall, C. striolatus venom was dominated by M, O1, O2, and A gene superfamily conotoxins and conkunitzins, which are potential modulators of sodium, calcium, and potassium channels. Conkunitzins and gene superfamily A peptides dominated the proximal over the distal duct, the M and O1 gene superfamily peptides were distributed along the full length of the duct, while the O2 gene superfamily peptides dominated the distal duct. Interestingly, the predatory injected venom of C. striolatus was dominated by peptides from gene superfamilies M, O1, O2, A, and conkunitzins, suggesting the predatory venom of C. striolatus may arise at multiple sites along the venom duct.

scholarly journals The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chao Peng ◽  
Yu Huang ◽  
Chao Bian ◽  
Jia Li ◽  
Jie Liu ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Cleavage Sites ◽  
Cone Snail ◽  
Post Translational Modifications ◽  
Peptide Processing ◽  
Central Dogma ◽  
Multiple Transcripts ◽  
Number Ratio ◽  
Addiction Therapy ◽  
Modified Peptides

AbstractAlthough there are various Conus species with publicly available transcriptome and proteome data, no genome assembly has been reported yet. Here, using Chinese tubular cone snail (C. betulinus) as a representative, we sequenced and assembled the first Conus genome with original identification of 133 genome-widely distributed conopeptide genes. After integration of our genomics, transcriptomics, and peptidomics data in the same species, we established a primary genetic central dogma of diverse conopeptides, assuming a rough number ratio of ~1:1:1:10s for the total genes: transcripts: proteins: post-translationally modified peptides. This ratio may be special for this worm-hunting Conus species, due to the high diversity of various Conus genomes and the big number ranges of conopeptide genes, transcripts, and peptides in previous reports of diverse Conus species. Only a fraction (45.9%) of the identified conotopeptide genes from our achieved genome assembly are transcribed with transcriptomic evidence, and few genes individually correspond to multiple transcripts possibly due to intraspecies or mutation-based variances. Variable peptide processing at the proteomic level, generating a big diversity of venom conopeptides with alternative cleavage sites, post-translational modifications, and N-/C-terminal truncations, may explain how the 133 genes and ~123 transcripts can generate thousands of conopeptides in the venom of individual C. betulinus. We also predicted many conopeptides with high stereostructural similarities to the putative analgesic ω-MVIIA, addiction therapy AuIB and insecticide ImI, suggesting that our current genome assembly for C. betulinus is a valuable genetic resource for high-throughput prediction and development of potential pharmaceuticals.

High-throughput proteomic analysis of human infiltrating ductal carcinoma of the breast

PROTEOMICS ◽  
2003 ◽  
Vol 3 (10) ◽  
pp. 1863-1873 ◽  
Author(s):  
Richard I. Somiari ◽  
Anthony Sullivan ◽  
Stephen Russell ◽  
Stella Somiari ◽  
Hai Hu ◽  
...  
Keyword(s):  
High Throughput ◽  
Proteomic Analysis ◽  
Ductal Carcinoma ◽  
Carcinoma Of The Breast ◽  
Infiltrating Ductal Carcinoma

scholarly journals Discovery Methodology of Novel Conotoxins from Conus Species

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 417 ◽  
Author(s):  
Ying Fu ◽  
Cheng Li ◽  
Shuai Dong ◽  
Yong Wu ◽  
Dongting Zhangsun ◽  
...  
Keyword(s):  
Drug Development ◽  
Gene Cloning ◽  
High Throughput ◽  
Disulfide Bond ◽  
Cone Snail ◽  
Crude Venom ◽  
Drug Candidates ◽  
Conus Species ◽  
New Drug Development ◽  
Natural Peptides

Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various Conus species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well.

scholarly journals Label-Free Whole Cell Biosensing for High-Throughput Discovery of Activators and Inhibitors Targeting G Protein-Activated Inwardly Rectifying Potassium Channels

ACS Omega ◽  
2018 ◽  
Vol 3 (11) ◽  
pp. 14814-14823 ◽  
Author(s):  
Katrin M. Krebs ◽  
Eva M. Pfeil ◽  
Katharina Simon ◽  
Manuel Grundmann ◽  
Felix Häberlein ◽  
...  
Keyword(s):  
Potassium Channels ◽  
G Protein ◽  
High Throughput ◽  
Label Free ◽  
Whole Cell ◽  
Inwardly Rectifying Potassium Channels ◽  
Inwardly Rectifying

scholarly journals High-Throughput LC–MS/MS Proteomic Analysis of a Mouse Model of Mesiotemporal Lobe Epilepsy Predicts Microglial Activation Underlying Disease Development

2016 ◽  
Vol 15 (5) ◽  
pp. 1546-1562 ◽  
Author(s):  
Vasiliki Bitsika ◽  
Venceslas Duveau ◽  
Julia Simon-Areces ◽  
William Mullen ◽  
Corinne Roucard ◽  
...  
Keyword(s):  
Mouse Model ◽  
High Throughput ◽  
Proteomic Analysis ◽  
Microglial Activation ◽  
Underlying Disease ◽  
Disease Development

scholarly journals Proteomic Analysis of the Ontogenetic Variability in Plasma Composition of Juvenile and Adult Bothrops jararaca Snakes

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Karen de Morais-Zani ◽  
Kathleen Fernandes Grego ◽  
Aparecida Sadae Tanaka ◽  
Anita Mitico Tanaka-Azevedo
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
2D Electrophoresis ◽  
Plasma Composition ◽  
Venom Composition ◽  
Bothrops Jararaca ◽  
Proteomic Approach ◽  
Adult Plasma ◽  
The Subject ◽  
Different Levels

The ontogenetic variability in venom composition of some snake genera, including Bothrops, as well as the biological implications of such variability and the search of new molecules that can neutralize the toxic components of these venoms have been the subject of many studies. Thus, considering the resistance of Bothrops jararaca to the toxic action of its own venom and the ontogenetic variability in venom composition described in this species, a comparative study of the plasma composition of juvenile and adult B. jararaca snakes was performed through a proteomic approach based on 2D electrophoresis and mass spectrometry, which allowed the identification of proteins that might be present at different levels during ontogenetic development. Among the proteins identified by mass spectrometry, antihemorrhagic factor Bj46a was found only in adult plasma. Moreover, two spots identified as phospholipase A2 inhibitors were significantly increased in juvenile plasma, which can be related to the higher catalytic PLA2 activity shown by juvenile venom in comparison to that of adult snakes. This work shows the ontogenetic variability of B. jararaca plasma, and that these changes can be related to the ontogenetic variability described in its venom.

scholarly journals High‐throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification

2019 ◽  
Vol 13 (11) ◽  
pp. 2305-2328 ◽  
Author(s):  
Yi Zhu ◽  
Tobias Weiss ◽  
Qiushi Zhang ◽  
Rui Sun ◽  
Bo Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Proteomic Analysis ◽  
Ffpe Tissue ◽  
Tissue Samples
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