Rapid Determination of H2S Poisoning in a Forensic Study Using a Novel Fluorescence Assay Based on Zn/Cu@BSA Nanoclusters

2018 ◽  
Vol 71 (3) ◽  
pp. 142 ◽  
Author(s):  
Lagabaiyila Zha ◽  
Weicheng Duan ◽  
Di Wen ◽  
Yadong Guo ◽  
Jie Yan ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Forensic Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Metal Nanoclusters ◽  
Fluorescence Sensing ◽  
Blood Samples ◽  
H2s Poisoning

The quantitative determination of H2S in the blood can provide valid evidence for H2S poisoning through occupational exposure. However, known traditional methods for the detection of H2S in blood are time consuming, require complicated pretreatments, and have low sensitivity. In this paper, a new fluorescence sensing assay is proposed for the rapid detection of H2S poisoning in forensic cases based on bovine serum albumin (BSA)-stabilised zinc/copper (Zn/Cu) bi-metal nanoclusters (Zn/Cu@BSA NCs). The as-prepared Zn/Cu@BSA NCs probes have been characterised by UV-vis absorption and fluorescence spectroscopy. The fluorescence of Zn/Cu@BSA NCs can be quenched through specific interactions between HS−/S2− and the Zn2+/Cu2+ bi-metal ions. Under optimised conditions, the fluorescence sensing method was linear in the concentration range of 2.5 nM to 30 mM with 0.69 nM as the limit of detection. Moreover, the practical feasibility of this fluorescence sensing method has also been demonstrated by the analysis of mice blood samples containing different levels of sulfide and human blood samples from forensic cases of H2S poisoning. Compared with gas chromatography/mass spectrometry (GC/MS), this fluorescence sensing method is quite simple, straightforward, and can be accurate for the quantitative determination of H2S poisoning in a few minutes for forensic analysis. Overall, this is the first report of a bi-metal fluorescence sensing assay for detecting H2S poisoning directly in blood. This research may provide a new approach for forensic toxicologists to monitor poisoning by H2S using a fluorescence-sensing method.

scholarly journals Optimized and Validated DBS/MAE/LC–MS Method for Rapid Determination of Date-Rape Drugs and Cocaine in Human Blood Samples—A New Tool in Forensic Analysis

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 249
Author(s):  
Paweł Stelmaszczyk ◽  
Ewa Gacek ◽  
Renata Wietecha-Posłuszny
Keyword(s):  
Date Rape ◽  
Rapid Determination ◽  
Extraction Process ◽  
Forensic Analysis ◽  
Blood Samples ◽  
Assisted Extraction ◽  
Wide Range ◽  
Human Blood Samples ◽  
Date Rape Drugs

The aim of this work was to develop a new method for the determination of selected substances from the date-rape drugs group: ketamine, benzodiazepines and cocaine. The method is based on the dried blood spot method which seems to be a suitable tool in the analysis of tested substances. The extraction process based on microwave-assisted extraction was optimized to enable optimal conditions for the isolation of a wide range of analytes from blood samples collected on DBS cards. The extraction with ethyl acetate with a buffer of pH = 9 carried out at a temperature of 50 °C for 15 min ensured high extraction efficiency of the tested analytes. The optimized method was validated. Limits of detection (LOD = 4.38–21.1 ng/mL) and quantification (LOQ = 14.6–70.4 ng/mL), inter- and intra-day precision (CV = 1.37–13.4% and 3.39–14.8%, respectively), recovery (RE = 93.0–112.4%) and matrix effect (ME = 98.4–101.6%) were determined. The validation results indicate the possibility of using the proposed method in the analysis of real blood samples collected from victims of sexual assault.

scholarly journals Rapid Determination of Ethylene Oxide and 75 VOCs in Ambient Air with Canister Sampling and Associated Growth Issues

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 35
Author(s):  
Jason Hoisington ◽  
Jason S. Herrington
Keyword(s):  
Ethylene Oxide ◽  
Rapid Determination ◽  
Complex Mixture ◽  
Ambient Air ◽  
Gas Chromatography Mass Spectrometry ◽  
Average Accuracy ◽  
Relative Standard ◽  
Wide Range ◽  
Us Epa

A canister-based sampling method along with preconcentrator-Gas chromatography-Mass Spectrometry (GC-MS) analysis was applied to ethylene oxide (EtO or EO) and 75 other volatile organic compounds (VOCs) in ambient air. Ambient air can contain a large variety of VOCs, and thorough analysis requires non-discriminatory sampling and a chromatographic method capable of resolving a complex mixture. Canister collection of whole air samples allows for the collection of a wide range of volatile compounds, while the simultaneous analysis of ethylene oxide and other VOCs allows for faster throughput than separate methods. The method presented is based on US EPA Method TO-15A and allows for the detection of EtO from 18 to 2500 pptv. The method has an average accuracy of 104% and precision of 13% relative standard deviation (RSD), with an instrument run time of 32 min. In addition, a link between canister cleanliness and ethylene oxide growth is observed, and potential mechanisms and cleaning strategies are addressed.

scholarly journals Rapid Determination of Cadmium Contamination in Lettuce Using Laser-Induced Breakdown Spectroscopy

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2930 ◽  
Author(s):  
Tingting Shen ◽  
Wenwen Kong ◽  
Fei Liu ◽  
Zhenghui Chen ◽  
Jingdong Yao ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Univariate Analysis ◽  
Laser Induced Breakdown Spectroscopy ◽  
Growth Environment ◽  
Breakdown Spectroscopy ◽  
Contamination Degree ◽  
Cd Contamination ◽  
Laser Induced Breakdown

Quick access to cadmium (Cd) contamination in lettuce is important to supervise the leafy vegetable growth environment and market. This study aims to apply laser-induced breakdown spectroscopy (LIBS) technology for fast determination of Cd content and diagnosis of the Cd contamination degree in lettuce. Emission lines Cd II 214.44 nm, Cd II 226.50 nm, and Cd I 228.80 nm were selected to establish the univariate analysis model. Multivariate analysis including partial least squares (PLS) regression, was used to establish Cd content calibration models, and PLS model based on 22 variables selected by genetic algorithm (GA) obtained the best performance with correlation coefficient in the prediction set Rp2 = 0.9716, limit of detection (LOD) = 1.7 mg/kg. K-Nearest Neighbors (KNN) and random forest (RF) were used to analyze Cd contamination degree, and RF model obtained the correct classification rate of 100% in prediction set. The preliminary results indicate LIBS coupled with chemometrics could be used as a fast, efficient and low-cost method to assess Cd contamination in the vegetable industry.

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