Comparing Self-Assembling and Covalent Fluorescent Boronolectins for the Detection of Free Sialic Acid

2011 ◽  
Vol 64 (11) ◽  
pp. 1454 ◽  
Author(s):  
Stephan M. Levonis ◽  
Milton J. Kiefel ◽  
Todd A. Houston
Keyword(s):  
Sialic Acid ◽  
Self Assembly ◽  
Free Sugars ◽  
System A ◽  
Self Assembling ◽  
Assembly Method ◽  
Aldehydes And Ketones ◽  
Aminophenylboronic Acid ◽  
Free Sialic Acid

A self-assembling fluorescence sensor with boronic acid functionalities was tested for binding selectivity to the monosaccharide, sialic acid. Working from a previously reported system, a self-assembling system could form an imine in situ that enables a conjugated fluorophore to display a measurable change in fluorescence in the presence of monosaccharide. However, further examination showed that free sugars give a similar fluorescence response to just the m-aminophenylboronic acid moiety on its own. Still, such a self-assembly method may be applicable to cell surface saccharide sensing as aldehydes and ketones are noticeably absent on most cells’ exteriors. The original covalent receptor appears best suited for the detection of free sialic acid.

Tandem Molecular Self-assembly for Selective Lung Cancer Therapy with Two Orders of Magnitude Increase in Efficiency

Nanoscale ◽  
2021 ◽  
Author(s):  
Debin Zheng ◽  
Jingfei Liu ◽  
Yinghao Ding ◽  
Limin Xie ◽  
Yingying Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Therapy ◽  
Anticancer Drugs ◽  
Self Assembly ◽  
Therapeutic Efficacy ◽  
Self Assembling ◽  
Lung Cancer Therapy ◽  
Magnitude Increase

In situ self-assembling of prodrug molecules into nanomedicine can elevate the therapeutic efficacy of anticancer medications by enhancing the targeting and enrichment of anticancer drugs at tumor sites. However, the...

scholarly journals Mitochondria-Targeted Self-Assembly of Peptide-Based Nanomaterials

2021 ◽  
Vol 9 ◽  
Author(s):  
Zhen Luo ◽  
Yujuan Gao ◽  
Zhongyu Duan ◽  
Yu Yi ◽  
Hao Wang
Keyword(s):  
Clinical Trials ◽  
Cancer Therapy ◽  
Self Assembly ◽  
Recent Progress ◽  
Assembly Systems ◽  
Targeted Cancer Therapy ◽  
Cancer Treatments ◽  
Self Assembling ◽  
Stimuli Response

Mitochondria are well known to serve as the powerhouse for cells and also the initiator for some vital signaling pathways. A variety of diseases are discovered to be associated with the abnormalities of mitochondria, including cancers. Thus, targeting mitochondria and their metabolisms are recognized to be promising for cancer therapy. In recent years, great efforts have been devoted to developing mitochondria-targeted pharmaceuticals, including small molecular drugs, peptides, proteins, and genes, with several molecular drugs and peptides enrolled in clinical trials. Along with the advances of nanotechnology, self-assembled peptide-nanomaterials that integrate the biomarker-targeting, stimuli-response, self-assembly, and therapeutic effect, have been attracted increasing interest in the fields of biotechnology and nanomedicine. Particularly, in situ mitochondria-targeted self-assembling peptides that can assemble on the surface or inside mitochondria have opened another dimension for the mitochondria-targeted cancer therapy. Here, we highlight the recent progress of mitochondria-targeted peptide-nanomaterials, especially those in situ self-assembly systems in mitochondria, and their applications in cancer treatments.

scholarly journals In situ self-assembly of zirconium metal–organic frameworks onto ultrathin carbon nitride for enhanced visible light-driven conversion of CO2 to CO

2020 ◽  
Vol 8 (12) ◽  
pp. 6034-6040 ◽  
Author(s):  
Yanan Wang ◽  
Wenlong Zhen ◽  
Yiqing Zeng ◽  
Shipeng Wan ◽  
Haiwei Guo ◽  
...  
Keyword(s):  
Visible Light ◽  
Self Assembly ◽  
Carbon Nitride ◽  
Metal Organic Framework ◽  
Metal Organic Frameworks ◽  
Assembly Method ◽  
Visible Light Driven ◽  
Metal Organic ◽  
Zirconium Metal

A series of Zr-porphyrin metal–organic framework (Zr-PMOF)/ultrathin g-C3N4 (UCN) heterostructure photocatalysts, as stable and efficient catalysts for the photoreduction of CO2, have been fabricated via a facile in situ hydrothermal self-assembly method.

Carbon Nanotubes-Assisted Fabrication of TiO2 Nanocomposite Film for Optimum Electrochromic Application

2014 ◽  
Vol 989-994 ◽  
pp. 789-792
Author(s):  
Shu Ping Liu ◽  
Wei Wang ◽  
Lin Lin Cui ◽  
Hua Nan Guan
Keyword(s):  
Carbon Nanotubes ◽  
Self Assembly ◽  
Multilayer Film ◽  
Great Promise ◽  
Layer By Layer ◽  
Electrochromic Devices ◽  
Optical Contrast ◽  
Coloration Efficiency ◽  
Assembly Method

Electrochromic composite film consisting of TiO2, chitosan (CS) and carbon nanotubes (CNTs) were fabricated on quartz and FTO substrates by the layer-by-layer self-assembly method (LbL). The multilayer film was characterized by UV-vis spectrum, scanning electron microscopy (SEM), cyclic voltammetry (CV) and chronoamperometric (CA) and in situ spectral electrochemicalmeasurements. The composite material shows high electrochromic performance, with the optical contrast of 11.5% and coloration efficiency of 21.7 cm2/C at 800 nm. The results indicate great promise for the TiO2-based film as a potential material in electrochromic devices.

Using organic solvent absorption as a self-assembly method to synthesize three-dimensional (3D) reduced graphene oxide (RGO)/poly(3,4-ethylenedioxythiophene) (PEDOT) architecture and its electromagnetic absorption properties

RSC Advances ◽  
2014 ◽  
Vol 4 (91) ◽  
pp. 49780-49782 ◽  
Author(s):  
Fan Wu ◽  
Yuan Wang ◽  
Mingyang Wang
Keyword(s):  
Organic Solvent ◽  
Self Assembly ◽  
Three Dimensional ◽  
Absorption Properties ◽  
Self Assembling ◽  
Assembly Method ◽  
Electromagnetic Absorption ◽  
Solvent Absorption ◽  
Reduced Graphene ◽  
Promising Application

A novel self-assembling 3D-RGO/PEDOT architecture has been synthesized through organic solvent absorption and gentle heating. It gives a promising application in electromagnetic absorption.

scholarly journals In situ kinetic measurements of α-synuclein aggregation reveal large population of short-lived oligomers

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245548
Author(s):  
Enrico Zurlo ◽  
Pravin Kumar ◽  
Georg Meisl ◽  
Alexander J. Dear ◽  
Dipro Mondal ◽  
...  
Keyword(s):  
Self Assembly ◽  
Large Population ◽  
Paramagnetic Resonance ◽  
Kinetic Measurements ◽  
Toxic Species ◽  
Self Assembling ◽  
Wide Range ◽  
Electron Paramagnetic

Knowledge of the mechanisms of assembly of amyloid proteins into aggregates is of central importance in building an understanding of neurodegenerative disease. Given that oligomeric intermediates formed during the aggregation reaction are believed to be the major toxic species, methods to track such intermediates are clearly needed. Here we present a method, electron paramagnetic resonance (EPR), by which the amount of intermediates can be measured over the course of the aggregation, directly in the reacting solution, without the need for separation. We use this approach to investigate the aggregation of α-synuclein (αS), a synaptic protein implicated in Parkinson’s disease and find a large population of oligomeric species. Our results show that these are primary oligomers, formed directly from monomeric species, rather than oligomers formed by secondary nucleation processes, and that they are short-lived, the majority of them dissociates rather than converts to fibrils. As demonstrated here, EPR offers the means to detect such short-lived intermediate species directly in situ. As it relies only on the change in size of the detected species, it will be applicable to a wide range of self-assembling systems, making accessible the kinetics of intermediates and thus allowing the determination of their rates of formation and conversion, key processes in the self-assembly reaction.

In Situ Self-Assembled Graphene-Polyaniline Nanocomposite Thin Films for Ammonia Gas Sensor

2014 ◽  
Vol 998-999 ◽  
pp. 63-66
Author(s):  
Ning Jie Guo ◽  
Hui Ling Tai ◽  
Zong Biao Ye ◽  
Guang Zhong Xie
Keyword(s):  
Gas Sensor ◽  
Self Assembly ◽  
Nanocomposite Film ◽  
Graphene Film ◽  
Ammonia Gas ◽  
Assembly Method ◽  
Vis Spectroscopy ◽  
Nanocomposite Thin Films ◽  
Polyaniline Nanocomposite

In this paper, the graphene-polyaniline (PANI) nanocomposite thin film was prepared by the in situ self-assembly method for the ammonia (NH3) gas sensor application, which was characterized by scanning electron microscopy (SEM) and UV-vis spectroscopy. The NH3 sensing performance and mechanism of the nanocomposite film were investigated. The results revealed that the sensor based on graphene-polyaniline nanocomposite film exhibited better sensing properties and restorability than those of single graphene film.

scholarly journals Bioinspired Photo-Crosslinkable Self-Assembling Peptide with pH-Switchable “On−Off” Luminescence.

2021 ◽  
Author(s):  
Raffaele Pugliese ◽  
Monica Montuori ◽  
Fabrizio Gelain
Keyword(s):  
Self Assembly ◽  
Cross Linking ◽  
Significant Progress ◽  
The Past ◽  
Self Assembling ◽  
Made In

Significant progress has been made in the peptides self-assembly over the past two decades, however the in situ cross-linking of self-assembling peptides yielding to better performing nanomaterials is still in...

scholarly journals Cross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System

2020 ◽  
Vol 21 (12) ◽  
pp. 4261
Author(s):  
Raffaele Pugliese ◽  
Fabrizio Gelain
Keyword(s):  
Mechanical Properties ◽  
Self Assembly ◽  
Self Healing ◽  
One Pot ◽  
Self Assembling ◽  
In Situ Gelation ◽  
Wide Range ◽  
Peptide Hydrogels ◽  
Peptide Hydrogel

Supramolecular nanostructures formed through peptide self-assembly can have a wide range of applications in the biomedical landscape. However, they often lose biomechanical properties at low mechanical stress due to the non-covalent interactions working in the self-assembling process. Herein, we report the design of cross-linked self-assembling peptide hydrogels using a one-pot in situ gelation system, based on 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide/N-hydroxysulfosuccinimide (EDC/sulfo–NHS) coupling, to tune its biomechanics. EDC/sulfo–NHS coupling led to limited changes in storage modulus (from 0.9 to 2 kPa), but it significantly increased both the strain (from 6% to 60%) and failure stress (from 19 to 35 Pa) of peptide hydrogel without impairing the spontaneous formation of β-sheet-containing nano-filaments. Furthermore, EDC/sulfo–NHS cross-linking bestowed self-healing and thixotropic properties to the peptide hydrogel. Lastly, we demonstrated that this strategy can be used to incorporate bioactive functional motifs after self-assembly on pre-formed nanostructures by functionalizing an Ac-LDLKLDLKLDLK-CONH2 (LDLK12) self-assembling peptide with the phage display-derived KLPGWSG peptide involved in the modulation of neural stem cell proliferation and differentiation. The incorporation of a functional motif did not alter the peptide’s secondary structure and its mechanical properties. The work reported here offers new tools to both fine tune the mechanical properties of and tailor the biomimetic properties of self-assembling peptide hydrogels while retaining their nanostructures, which is useful for tissue engineering and regenerative medicine applications.

FORMATION OF REVERSED MICELLE NANORING BY A DESIGNED SURFACTANT-LIKE PEPTIDE

NANO ◽  
2012 ◽  
Vol 07 (04) ◽  
pp. 1250024 ◽  
Author(s):  
FENG QIU ◽  
YONGZHU CHEN ◽  
CHENGKANG TANG ◽  
YANRONG LU ◽  
JINGQIU CHENG ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Self Assembly ◽  
Molecular Model ◽  
Intermolecular Hydrogen Bond ◽  
Reversed Micelle ◽  
System A ◽  
Self Assembling ◽  
Potential Applications ◽  
Ft Ir ◽  
Novel Strategy

Designing self-assembling peptides as nanomaterials has been an attractive strategy in recent years, however, these peptides were usually studied in aqueous solutions for their self-assembling behaviors and applications. In this study, we have designed a surfactant-like peptide AGD with a wedge-like shape and studied its self-assembling behaviors in aqueous solution or nonpolar system. By analyzing the intermolecular hydrogen bond using FT-IR and characterizing the nanostructures with DLS, AFM and TEM, it was confirmed that AGD could not undergo self-assembly in aqueous solution while could self-assemble into well-ordered nanorings in nonpolar system. A molecular model has been proposed to explain how the nanorings were formed in the manner of reversed micelle. These results suggested a novel strategy to fabricate self-assembling peptide nanomaterials in nonpolar system, which could have potential applications in many fields.

Sign in / Sign up

Export Citation Format

Share Document