Triplex Formation by an Oligonucleotide Containing the Novel Modified Nucleoside 2′-Deoxy-N4-phenylcarbamoylcytidine

1998 ◽  
Vol 51 (11) ◽  
pp. 965 ◽  
Author(s):  
Nancy Guzzo-Pernell ◽  
John M. Lawlor ◽  
Geoffrey W. Tregear ◽  
Jim Haralambidis
Keyword(s):  
Base Pair ◽  
Thermal Denaturation ◽  
The Novel ◽  
The Third ◽  
Ph Values ◽  
Triplex Formation ◽  
Guanine Base ◽  
Modified Nucleoside

2′-Deoxy-N4-phenylcarbamoylcytidine (Pc) was designed for triplex recognition of the cytosine{guanine base pair at physiological pH values to assist in the development of triplex-forming oligomers. Thermal denaturation of a triplex mixture in which the third strand incorporated Pc as a central nucleoside showed that, at pH 6·1, Pc binds selectively—though weakly—to the target cytosine—guanine and also to guanine—cytosine; but the gel mobilities of the same mixtures indicate the possibility of specificity for the target cytosine-guanine base pair at pH values higher than 6·1. The result may provide leads useful in the development of improved triplex-forming oligomers.

scholarly journals Thermal denaturation in acidic solutions of double-helical ribonucleic acid from virus-like particles found in Penicillium chrysogenum. A spectrophotometric study

1971 ◽  
Vol 125 (2) ◽  
pp. 655-665 ◽  
Author(s):  
R. A. Cox ◽  
K. Kanagalingam ◽  
Elisabeth Sutherland
Keyword(s):  
Base Pair ◽  
Penicillium Chrysogenum ◽  
Sodium Phosphate ◽  
Thermal Denaturation ◽  
Spectrophotometric Study ◽  
Base Pairs ◽  
Native Form ◽  
Acidic Solutions ◽  
Ph Values ◽  
Sodium Phosphate Buffer

1. Two species of double-helical RNA isolated from mycelium of Penicillium chrysogenum were titrated with acid at 25°C and 95°C (solvent 0.1m-sodium phosphate buffer). At 25°C denaturation occurred at about pH3. At 95°C in the denatured form cytosine residues titrated as a simple monobasic acid of pK3.9 compared with pK≃2.5 for the native form at 25°C. 2. On thermal denaturation in neutral and acidic solutions one species of RNA (38% rG·rC) ‘melted’ in three distinct stages, equivalent to a mixture of three species, namely one of about 25% rG·rC, another of about 33% rG·rC and a third of about 46% rG·rC: the relative proportions were 0.25:0.35:0.40. 3. On thermal denaturation in acidic solutions the increase in the fraction of ionized cytosine residues concomitant with the ‘melting’ of rG·rC base pair also affects the spectrum especially at 280nm and serves to enhance the contribution of rG·rC base pairs at this wavelength. The increment in ε(P) at 280nm on ‘melting’ an rG·rC base pair approaches 53501·mol−1·cm−1 depending on pH, compared with 33501·mol−1·cm−1 at pH7. In contrast ε(P) at 280nm is scarcely affected by ‘melting’ rA·rU base pairs or by the protonization of adenine residues. 4. Changes in the spectrum of Escherichia coli rRNA on denaturation in acidic solutions were studied to yield the mole fractions of rA·rU and rG·rC base pairs ‘melting’ at particular pH values.

Feelings of Figures in Novel Notes Juang Fiersa Besari (Personality Ludwig Klages)

2020 ◽  
Vol 8 (3) ◽  
pp. 13
Author(s):  
Faridatus Soleha
Keyword(s):  
Qualitative Approach ◽  
Personality Structure ◽  
Personality Theory ◽  
The Novel ◽  
Research Focus ◽  
The Third ◽  
Analyze Data

This study aims to describe the feelings of the characters in the novel Juang Notes by Fiersa Bersari and researchers analyze using personality theory originated by Ludwig Klages by focusing his study on the personality structure of feelings. Feeling is a process of someone accepting or rejecting something in life. This study uses a qualitative approach by using library techniques to obtain data that will produce a description of the words or sentences in the observed study. In the analysis of this research using the hermeutics technique, in the hermeutics technique there are several stages, namely reading the research object in this study in the form of a fighting journal, the second gives a mark on the data that has been obtained from the reading results, the third provides code or coding on the data that has been found, and the fourth is to analyze data that has been obtained from the object of research in accordance with the specified research focus. Novel Notes Juang by Fiersa Besari is a novel that can be used as an inspiration for readers in living life. Based on the results of the study it was found that in the Fighting Notes novel there is a feeling that is divided into inner activities and the level of clarity, inner activities in the novel in the form of fear and guilt while viewed from the level of clarity in the form of happiness, sadness and longing.

The Coherence of Mary Barton: Romance, Realism, and Utopia

2015 ◽  
Vol 5 (3) ◽  
pp. 185-200
Author(s):  
Robert Z. Birdwell
Keyword(s):  
Thomas Carlyle ◽  
The Novel ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Three Stages ◽  
Mode Of Production

Critics have argued that Elizabeth Gaskell's first novel, Mary Barton (1848), is split by a conflict between the modes of realism and romance. But the conflict does not render the novel incoherent, because Gaskell surpasses both modes through a utopian narrative that breaks with the conflict of form and gives coherence to the whole novel. Gaskell not only depicts what Thomas Carlyle called the ‘Condition of England’ in her work but also develops, through three stages, the utopia that will redeem this condition. The first stage is romantic nostalgia, a backward glance at Eden from the countryside surrounding Manchester. The second stage occurs in Manchester, as Gaskell mixes romance with a realistic mode, tracing a utopian drive toward death. The third stage is the utopian break with romantic and realistic accounts of the Condition of England and with the inadequate preceding conceptions of utopia. This third stage transforms narrative modes and figures a new mode of production.

Representasi Sikap Kebangsaan Anak Muda dalam Hegemoni Negara pada Novel Populer

Metahumaniora ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 100
Author(s):  
Muhamad Adji
Keyword(s):  
New Order ◽  
The Novel ◽  
The Third ◽  
Study Results ◽  
Popular Novel

AbstrakPenelitian ini bertujuan untuk memperoleh gambaran tentang sikap kebangsaananak muda Indonesia pada tiga novel populer yaitu Ali Topan Anak Jalanan karya TeguhEsha, Lupus: Makhluk Manis dalam Bis karya Hilman Hariwijaya, dan Balada Si Roy karya GolaGong. Alasan pemilihan objek penelitian di atas adalah karena ketiga novel tersebut memilikitingkat popularitas yang tinggi pada tiap zamannya sehingga membuat tokoh utamanyamenjadi representasi anak muda Indonesia pada zamannya masing-masing. Pertanyaanpertanyaanyang memandu tulisan ini adalah 1) Bagaimana keindonesiaan digambarkanpada ketiga novel tersebut dan 2) Bagaimana sikap kebangsaan direpresentasikan tokohanak muda pada ketiga novel tersebut. Dari hasil kajian didapatkan simpulan bahwasikap kebangsaan yang ditampilkan pada ketiga novel tersebut memiliki tingkatan yangberbeda-beda. Novel Ali Topan Anak Jalanan menunjukkan sikap kebangsaan dengan lebihkritis. Hegemoni orde Baru pada masa itu belum berhasil karena novel populer masihmenampilkan tokoh anak muda yang kritis dan cenderung memberontak terhadap nilainilaiideologi Orde Baru. Periode ’80-an menampilkan novel populer dengan tokoh anakmuda yang memiliki sikap kebangsaan yang terbelah. Pertama, anak muda yang dapatmenerima realitas yang dikontruksi Orde Baru, dengan menjalani sistem tersebut danmemposisikan dirinya sebagai bagian dari sistem tersebut. Hal itu direpresentasikan olehtokoh Lupus dalam novel Lupus: Makhluk Manis dalam Bis. Kedua, anak muda yang tidakdapat menerima realitas yang dikonstruksi Orde Baru, dengan cara keluar dari bagiansistem tersebut. Hal itu direpresentasikan oleh tokoh Roy dalam Balada Si Roy.Kata kunci: kebangsaan, anak muda, novel populer, Orde BaruAbstractThis study aims to obtain a picture of Indonesian nationalism in the three popularnovels that were Ali Topan Anak Jalanan by Teguh Esha, Lupus: Makhluk Manis dalam Bisby Hilman Hariwijaya, dan Balada Si Roy by Gola Gong. The reason for choosing the object ofresearch above is that all three novels are recognized to have high levels of popularity in eachera so that made the main characters became a representation of Indonesian youth at that time.The questions that guide the writing are 1) How Indonesianness described in the third novel?2) How does nationalism represented by youth in these three novels. From the study results isobtained the conclusion that nationalism is displayed on the third novel has a level different.Ali Topan Anak Jalanan show a nationalism more critically. The New Order’s hegemony atthat time has not been successful because the novel still showing a critical youth and tends torevolt against the ideological values of the New Order. The ‘80s period featured a popular novelwith a youth character who had a split nationalism. First, the youth who can accept the realitythat the New Order has constructed, with the system and position itself as part of the system.It was represented by the Lupus’s character in the Lupus: Makhluk Manis dalam Bis. Second,the youth who can not accept the facts constructed by the New Order, by going out from thatpart of the system. It was represented by Roy’s character in the Balada Si Roy.Keywords: nationalism, youth, popular novel, New Order

The Limit Situation And The Narrative Of War (On The Material Of The Novel “My Lieutenant” By Daniil Granin)

2020 ◽  
Vol 6 (1) ◽  
pp. 90-104
Author(s):  
Anatoly S. Kuprin ◽  
Galina I. Danilina
Keyword(s):  
20Th Century ◽  
Scientific Work ◽  
Point Of View ◽  
Continuous Limit ◽  
The Novel ◽  
Term Limit ◽  
Abstract Author ◽  
The Third ◽  
Definition Of

The purpose of this study is the analysis of limit situation in the narrative of war. The material of the study is the novel of Daniil Granin “My Lieutenant” and related texts. In the first part of the paper, the authors explore existing approaches to the term “limit situation” and similar concepts into scientific and philosophical traditions; limits of its applicability in literary studies and its relation to the categories of “narrative instances” and “event”. Proposed a literary-theoretical definition of the limit situation, which can be used in the analysis of fiction texts. Existing approaches to the examination of the situation of war are analyzed: philosophical-existential, psychoanalytic, sociological, literary. In the second part of the paper, the authors propose their method for analyzing limit situations in texts about war, which basis on existing approaches and preserves the text-centric principle of studying the structure of the story. Two interrelated areas of research have been identified: the study of war as a continuous limit situation in the intertextual aspect (the discourse of war); the study of limit situations (death, suffering, guilt, accident) in the narrative of war as part of a specific text. In the third part of the scientific work,the analysis of war as a continuous limit situation results in the study of the concept of “limit” (border) in a fiction text. The role of “limit” (border) concept in the texts about the war is studied, the possible types of limits in the discourse of war are examined. Limit situations in the narrative of war are analyzed on the basis of the novel “My Lieutenant” by Daniil Granin. A review of journalistic and scientific works about the novel revealed both the continuity and the differences between the novel and the “lieutenant” prose of the 20th century. An analysis of the limit situations in the novel revealed their key position in the narrative. These situations are independent of the fiction time, of the fluctuation of the point of view’; the function of the abstract author is to build the narrative as a “directive” immersion of the hero and narrator in these situations.

The Free Responsible Action of the Individual

2018 ◽  
Author(s):  
Michael P. DeJonge
Keyword(s):  
The Novel ◽  
Extreme Situation ◽  
The Third ◽  
Third Phase ◽  
Responsible Action ◽  
Action Type ◽  
Two Phases ◽  
The Individual ◽  
The Church

If, as Chapter 12 argues, much of Bonhoeffer’s resistance thinking remains stable even as he undertakes the novel conspiratorial resistance, what is new in his resistance thinking in the third phase? What receives new theological elaboration is the resistance activity of the individual, which in the first two phases was overshadowed by the resistance role played by the church. Indeed, as this chapter shows, Bonhoeffer’s conspiratorial activity is associated with what he calls free responsible action (type 6), and this is the action of the individual, not the church, in the exercise of vocation. As such, the conspiratorial activity is most closely related to the previously developed type 1 resistance, which includes individual vocational action in response to state injustice. But the conspiratorial activity differs from type 1 resistance as individual vocational action in the extreme situation.

Richardson's Pamela: An Interpretation

PMLA ◽  
1973 ◽  
Vol 88 (1) ◽  
pp. 79-91
Author(s):  
Stuart Wilson
Keyword(s):  
Moral Principle ◽  
The Novel ◽  
The Third ◽  
Narrative Process

The heroine of Richardson's first novel is neither a meretricious young hussy nor a paragon of virtue; she is a complex personality who moves from a naive adolescence to a composed maturity in the course of the narrative. The conflict between her devotion to moral principle and her growing affection for Mr. B., which develops in the first, or Bedfordshire, section of the novel, brings a near-psychic collapse in the second, or Lincolnshire, section. The imagery and symbolism show the nature of her torments, her growing awareness of a love that combines eros and agape, and her need for the reconciliation between conscience and libido which is completed after her return to the Bedfordshire estate in the third section. The formal symmetry of the novel evolves from the narrative process within which Pamela is tested and proved capable of an honest love and a tranquil marriage.

scholarly journals Design of an ultimate quencher free molecular beacon containing pyrrolocytidine-guanine base pair

2008 ◽  
Vol 52 (1) ◽  
pp. 361-362 ◽  
Author(s):  
Y. Saito ◽  
Y. Shinohara ◽  
S. S. Bag ◽  
Y. Takeuchi ◽  
K. Matsumoto ◽  
...  
Keyword(s):  
Base Pair ◽  
Molecular Beacon ◽  
Guanine Base

scholarly journals Sources of anthroponyms in P. Kulish’s novel “Chorna Rada”

2020 ◽  
pp. 53-60
Author(s):  
Oles Fedoruk
Keyword(s):  
Real Life ◽  
Close Friend ◽  
The Novel ◽  
The Third ◽  
Russian Novel ◽  
Different Sources

The paper analyzes different sources of anthroponyms in the original and final texts of P. Kulish’s novel “Chorna Rada: Khronika 1663 Roku” (“The Black Council: A Chronicle of the Year 1663”). Three types of sources have been identified: the historical prototypes, names and surnames of Kulish’s friends, and archival (documentary) records. In addition, numerous notes in the early editions of the Russian novel contain references to the works of various people (M. Markevych, D. Bantysh-Kamenskyi, V. Kokhovskyi, etc.). The last group of anthroponyms stands outside of the plot, and the paper does not focus on it. The historical and autobiographical sources of anthroponyms are generally known. Among the first are prototypes of two hetmans — Yakym Somko and Ivan Briukhovetskyi, military secretary M. Vukhaievych, regimental osaul M. Hvyntovka. The second group comprises the occasional characters Hordii Kostomara (a historian M. Kostomarov), Ivan Yusko (a teacher I. Yuskevych-Kraskovskyi), Hulak (M. Hulak, the founder of The Brotherhood of Saints Cyril and Methodius), Bilozerets (Kulish’s brother-in-law V. Bilozerskyi), Petro Serdiuk (Kulish’s close friend Petro Serdiukov), Oleksa Senchylo (teacher Oleksa Senchylo-Stefanovskyi). In the novel, Kulish drew the love line as a projection of his relationship with Oleksandra Bilozerska and her mother Motrona. The characters of Petro Shramenko, Lesia Cherevanivna and her mother Melaniia have an autobiographical basis. Accordingly, Lesia’s name was also taken from real life. The third group of sources supplying the anthroponyms is archival records. The paper analуzes Kulish’s extracts from the roster of Cossack regiments of the Hetmanate (1741). This source wasn’t used previously. It contains the anthroponyms Vasyl Nevolnyk (‘Slave’), Puhach, Petro Serdiuk, Taranukha, Chepurnyi, Cherevan, Tur, Shramko and Shramchenko, Shkoda, which the author used in various editions of the novel.

scholarly journals A glossary as an explicative translation technique in the translation of the third culture elements

2021 ◽  
Vol 145 ◽  
pp. 129-144
Author(s):  
Michał Gąska
Keyword(s):  
Literary Translation ◽  
Cultural Barriers ◽  
The Novel ◽  
Third Culture ◽  
Source Text ◽  
Translation Process ◽  
East Indies ◽  
The Third ◽  
Translation Technique ◽  
Similarities And Differences

Utilising notes or glossaries in literary translation has both its opponents and supporters. While the former conceive it as a translator’s helplessness and failure, the latter defend it as a manner of overcoming cultural barriers. The present article aims to scrutinize glossaries used as an explicative translation technique with regard to the rendering of the third culture elements. The analysis is conducted on the basis of the novel by Dutch writer Hella S. Haasse: Sleuteloog, in which the action is set in the Dutch East Indies. For this reason, Indonesian culture occurs as the third culture in the translation process. The source text is juxtaposed with its translations into German and Polish in order to examine the similarities and differences in images of the third culture elements the glossaries evoke in the addressees of the target texts.

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